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Screening of miRNAs Related to High Temperature Stress in Maize Pollen and Analysis of Their Target Genes LI Chuan, ZHANG Panpan, ZHANG Meiwei, NIU Jun, MU Weilin, GUO Hanxiao, QIAO Jiangfang Journal of Henan Agricultural Sciences    2024, 53 (2): 1-16.   DOI: 10.15933/j.cnki.1004-3268.2024.02.001 Abstract （2872）      PDF （4835KB）（635）       Knowledge map    Save By using high⁃throughput miRNA sequencing technology，differentially expressed miRNAs were indentified in pollens of high heat tolerant maize variety Zhengdan 958 and low heat tolerant maize variety Xianyu 335 under high temperature stress at flowering stage，their target genes were predicted，and the enrichment analysis of genetic characteristics and metabolic pathways of these target genes were conducted. The results showed that a total of 818 miRNA precursor sequences were identified. Nineteen significantly differentially expressed miRNA sequences were screened in the group of Zhengdan 958 pollen under high temperature stress compared with the control pollen under normal condition(HT958 vs CK958). Among them，15 miRNA sequences were up⁃regulated，4 miRNA sequences were down⁃regulated，and expression level difference of 3 miRNA sequences reached extremely significant level(P<0.01).The predicted results of target genes of 19 differentially expressed miRNA sequences showed that a total of 503 gene transcripts were obtained，their enriched GO biological process terms included regulation of transcription，DNA⁃templated，microtubule⁃based process，hosphorylation，positive regulation of transcription by RNA polymerase Ⅱ ，methylation and so on，and the most significantly enriched KEGG pathways were glutathione metabolism，carbon metabolism，arachidonic acid metabolism lycolysis/gluconeogenesis，folate biosynthesis and so on. Fiveteen significantly differentially expressed miRNA sequences were identified in the group of Xianyu 335 pollen under high temperature stress compared with the control pollen(HT335 vs CK335). Among them，7 miRNA sequences were up⁃regulated,8 were down⁃regulated，and the expression level difference of 1 miRNA sequence reached highly significant level(P<0.01).The predicted results of target genes of 15 differentially expressed miRNA sequences showed that a total of 454 gene transcripts were obtained，their enriched GO biological process terms included regulation of transcription，DNA⁃templated，phosphorylation，protein phosphorylation,proteolysis，DNA repair and so on，and the most significantly enriched KEGG pathways were other glycan degradation,linoleic acid metabolism,metabolic pathways，thiamine metabolism，protein processing in endoplasmic reticulum and so on.Eighty⁃five significantly differentially expressed miRNA sequences were identified in the group of Zhengdan 958 pollen under high temperature stress compared with Xianyu 335 pollen under high temperature stress(HT985 vs HT335). Among them，35 miRNA sequences were up⁃regulated，50 were down⁃regulated，and expression level difference of 24 miRNA sequences reached extremely significant level(P<0.01). The predicted results of target genes of 85 differentially expressed miRNA sequences showed that a total of 2 286 gene transcripts were obtained，their enriched GO biological process terms included regulation of transcription，DNA⁃templated，phosphorylation,protein phosphorylation,proteolysis,transmembrane transport and so on，the most significantly enriched KEGG pathways were sphingolipidmetabolism，starch and sucrose metabolism，other glycan degradation，metabolic pathways，cysteine and methionine metabolism and so on. Ninety⁃four significantly differentially expressed miRNA sequences were identified in the HT958 vs CK958 group compared with HT335 vs CK335 group. The predicted results of target genes of 94 differentially expressed miRNA sequences showed that a total of 4 569 gene transcripts were obtained.Among them，the expression level difference of 28 miRNA sequences showed extremely significant(P<0.01),including PC⁃3p⁃10069_1143C⁃3p⁃18335_646，zma⁃miR164f⁃5p and so on. The enriched GO biological process terms of target genes of 94 differentially expressed miRNA sequences included regulation of transcription,DNA⁃templated,phosphorylation,protein phosphorylation,protein transport,proteolysis and so on,and the most significantly enriched KEGG pathways were protein processing in endoplasmic reticulum，ribosome biogenesis in eukaryotes，spliceosome，sphingolipid metabolism，endocytosis and so on. Reference | Related Articles | Metrics

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Identification and Evaluation of Resistance to Stripe Rust of 480 Wheat Germplasms DAI Ziju, LI Wenxu, YANG Huimin, ZHU Xiuhua, WANG Yahuan, XU Fuxin, LIU Dongyang, HOU Jinna, QIN Maomao, WU Zhengqing, ZHOU Zhengfu, LEI Zhensheng Journal of Henan Agricultural Sciences    2024, 53 (9): 1-15.   DOI: 10.15933/j.cnki.1004-3268.2024.09.001 Abstract （2474）      PDF （1619KB）（715）       Knowledge map    Save The main purpose of the present study is to understand the resistance to stripe rust and the utilization of stripe rust resistant genes in current bred wheat varieties,and find the varieties containing novel stripe rust resistant loci.Totally,480 domestic and foreign wheat germplasms were collected from various planting regions.The varieties were inoculated with mixed races of CYR32,CYR33 and CYR34 for testing the adult plant resistance.Simultaneously,the resistance to leaf rust and powdery mildew was investigated in infected field.The KASP markers closely linked or co‑segregated with stripe rust,leaf rust and powdery mildew genes,Yr18/Lr34/Sr57/Pm38(7DS),Yr29/Lr46/Pm39/Sr58(1BL)and Yr30/Lr27/Sr2/Sb3(3BS),were selected for identifying the previous reported pleiotropic resistant genes.The results showed that there were 35(accounting for 7.29%)germplasms showed immunization to CYR32,CYR33 and CYR34,while the numbers of high‑resistant and medium‑resistant germplasms were 69(14.38%)and 79(16.46%),respectively.And the other 297(61.88%)germplasms demonstrated medium‑ or high‑ susceptible to the inoculated races.There were 13,10 and 7 germplasms containing the Yr18,and Yr29 and Yr30 genes,respectively;only 3 germplasms contained two of the resistant genes;no germplasm was detected with all the three resistant genes.In conclusion,183(accounting for 38.12%)germplasms showed resistant to stripe rust at adult plant stage,and 27 germplasms were detected with the above resistant genes.Yr18 or Yr30 gene showed resistant to the current races of stripe rust,however,the resistance level of Yr29 gene was partly lost at adult plant stage. Reference | Related Articles | Metrics

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Expression and Functional Analysis of Maize Stress Response Gene ZmTPR1 CAO Liru, LIANG Xiaohan, MA Chenchen, YE Feiyu, PANG Yunyun, LI Weiya, ZHANG Xin, LU Xiaomin Journal of Henan Agricultural Sciences    2024, 53 (1): 12-21.   DOI: 10.15933/j.cnki.1004-3268.2024.01.002 Abstract （2359）      PDF （2292KB）（310）       Knowledge map    Save On the basis of previous transcriptome sequencing of maize under drought‑rehydration treatment，ZmTPR1 （tetratricopeptide repeat 1）was identified in response to drought stress.Bioinformatics analysis was conducted，and expression pattern was analyzed in different tissues and under different stresses. The homologous gene AtTPR1 in Arabidopsis thaliana was knocked out by CRISPR‑Cas9 technology，the phenotype，physiological and biochemical indexes of homozygous mutant plants were analyzed under drought stress，and the function of this gene was preliminarily explored，which would provide genetic resources for breeding drought‑resistant maize varieties.The results showed that ZmTPR1 gene was located on chromosome 3 of maize，encoding 421 amino acids，containing a conserved coiled coil domain，and might be involved in the response of maize to plant hormones，drought and so on.ZmTPR1 gene expressed in all tissues of maize，with the highest expression level in young stems.Drought，high temperature，salt and nitrogen deficiency stresses could induce the expression of ZmTPR1 gene，and the expression level of ZmTPR1 gene was most up‑regulated after drought stress.The expression level of ZmTPR1 gene in drought‑resistant maize inbred line Zheng 36 was significantly higher than that in drought‑sensitive maize inbred line B73 after drought stress. Drought resistance of Arabidopsis thaliana decreased after AtTPR1 gene was knocked out，the growth of Attpr1 mutant was severely inhibited under drought stress，and the leaves were wilted or even dried out. At the same time，the relative water content，chlorophyll content，net photosynthetic rate，activities of peroxidase and superoxide dismutase significantly decreased，and the content of malondialdehyde significantly increased.In summary，ZmTPR1 gene is involved in the response of maize to various abiotic stresses，and plays an important role in drought stress. Reference | Related Articles | Metrics

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Research Progress of Porcine Getah Virus ZHANG Yuyang Journal of Henan Agricultural Sciences    2025, 54 (9): 15-22.   DOI: 10.15933/j.cnki.1004-3268.2025.09.002 Abstract （2124）      PDF （1296KB）（3981）       Knowledge map    Save Getah Virus（GETV） is an emerging insect‑borne pathogen that has caused reproductive disorders in pigs and high mortality in piglets in many countries in recent years，posing a major threat to global animal husbandry and public health. This virus is primarily transmitted by mosquito bites，which can cause widespread infection in pigs and significantly affect reproductive performance and health.In response to the spread of the epidemic，it is urgent to conduct research on genomic characterization and molecular evolution to guide vaccine design and optimize prevention and control strategies.At the same time，it is necessary to clarify the law of GETV transmission through epidemiological investigation and analyze the pathogenic mechanism in order to provide theoretical support for epidemic prevention and control.In addition，improving the accuracy and timeliness of early diagnosis has become a key link in prevention and control.In view of the critical role of early diagnosis in epidemic prevention and control，based on the latest research results，the pathogenic characteristics，epidemiological characteristics，pathogenic mechanism，diagnostic techniques and prevention and control strategies of GETV were systematically discussed，aiming to provide theoretical support for clinical prevention and control，and provide scientific basis for vaccine development，diagnostic technology innovation and prevention and control policy formulation. Reference | Related Articles | Metrics

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Comprehensive Evaluation of Stem Lodging Resistance of 145 Maize Inbred Lines ZHANG Fengqi, ZHANG Jun, MA Zhiyan, WU Xiaopan, ZHOU Qingwei, MU Xinyuan, TANG Baojun, LI Xin, XIA Laikun, DING Yong Journal of Henan Agricultural Sciences    2024, 53 (11): 34-40.   DOI: 10.15933/j.cnki.1004-3268.2024.11.004 Abstract （2097）      PDF （2064KB）（348）       Knowledge map    Save The plant height(PH),ear height(EH),stem rind penetrometer resistance(RPR),internode diameter(ID)and internode length(IL)were investigated under multiple environments with 145 maize inbred lines from home and abroad as materials.The excellent inbred lines with strong stem lodging resistance were screened out through correlation analysis,principal component analysis and cluster analysis,so as to provide excellent germplasm resources for maize lodging resistance breeding.The results indicated that PH,EH,RPR,ID and IL were extremely significantly different among different maize inbred lines.The heritability of PH with 0.99 was the highest,followed by EH and RPR all with 0.97.The correlation analysis results showed that RPR was extremely significantly positively correlated with PH.The principal component analysis results showed that the first three principal components represented 77.02% of the information of the five traits of 145 maize inbred lines,and their contribution rates were 37.08%,21.94% and 18.00%,respectively. Based on the comprehensive evaluation and cluster analysis of lodging resistance of each inbred line,145 inbred lines were divided into 6 groups,13 inbred lines with strong lodging resistance,such as S268,S112,S135,S270,etc,were screened out. Reference | Related Articles | Metrics

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Screening of Cellulose and Lignin Degrading Bacteria and Their Effect on Composting of Mushroom Residue WANG Xianxin, QI Lihua, SONG Jiangfu, ZHANG Qingge, LIU Congcong, ZHANG Qi, SONG Feifei, XU Ailing Journal of Henan Agricultural Sciences    2024, 53 (9): 66-79.   DOI: 10.15933/j.cnki.1004-3268.2024.09.007 Abstract （1962）      PDF （6107KB）（2116）       Knowledge map    Save In order to screen high‑efficiency composite bacteria that promote the degradation of cellulose and lignin in the composting process of mushroom residue,sodium carboxymethyl cellulose plate method,Congo red staining method and lignin plate method were used for preliminary screening，combined with filter paper strip disintegration test,aniline blue decolorization rate determination and cellulose and lignin enzyme activity determination test for rescreening from the mushroom residue waste,and then they were used for aerobic composting test to study the physical and chemical parameters and microbial community changes in the process,and to evaluate their degradation ability of cellulose and lignin in mushroom residue.The results showed that the enriched cellulose‑degrading bacteria could completely degrade the filter paper within 6 days,and the activities of carboxymethyl cellulase(CMCase),filter paper enzyme(FPA),exoglucanase(C1)and β‑glucosidase(β‑Gase)reached 2.50,7.30,3.43 and 2.90 U/mL,respectively.The maximum decolorization rate of aniline blue reached 92.10% within 5 days by enriched lignin degrading bacteria，and the activities of lignin peroxidase(Lac),laccase(Lip)and manganese peroxidase(Mnp)reached 0.80,2.42 and 1.35 U/mL,respectively.The results of composting test showed that compared with the natural composting treatment(CK),the highest temperature of the pile body was 58.77℃,and the high temperature(>55℃)lasted for 5 days.The relative decomposition rate of cellulose and lignin increased by 35.83% and 54.33% respectively.The addition of microbial agents increased the richness of bacterial community in the middle stage of composting,and the relative abundance of Pseudomonas,Idiomarina,lutamicibacter,Thiopseudomonas,Globicatella,Pusillimonas,Lysobacter and Brumimicrobium increased. Redundancy analysis(RDA)results showed that temperature,pH value and electrical conductivity(EC value)were the significant factors affecting the composition of bacterial community in the middle stage of composting.In summary,the composite degrading bacteria screened in this study can effectively degrade cellulose and lignin,and prolong the high temperature period of composting Reference | Related Articles | Metrics

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Establishment of Callus Induction and Regeneration System of Cyperus esculentus ZHU Yajing, ZHANG Xiangge, XU Xinran, HU Haiyan, CHENG Shan, CHEN Chen, LI Chunxin, SONG Wanxian, SONG Wenxu, WANG Huiwei Journal of Henan Agricultural Sciences    2024, 53 (10): 73-81.   DOI: 10.15933/j.cnki.1004-3268.2024.10.009 Abstract （1945）      PDF （3147KB）（762）       Knowledge map    Save In order to establish an efficient callus induction and regeneration system of Cyperus esculentus,the explants（young root,young leaf,young leaf sheath and tillering node)for callus induction were screened,and the effects of different plant growth regulator combinations on callus induction,proliferation and differentiation were explored.The results showed that the best explant for callus induction of Cyperus esculentus was tillering node;the optimal plant growth regulator combination for callus induction was 2.5 mg/L 2,4⁃D+0.6 mg/L 6⁃BA+0.6 mg/L NAA+0.9 mg/L ZT,and the induction rate could reach 65.58%;the optimal plant growth regulator combination for callus proliferation was 0.5 mg/L 2,4⁃D+0.9 mg/L 6⁃BA+1.2 mg/L NAA+1.2 mg/L ZT,and the proliferation rate could reach 68.12%;the optimal plant growth regulator combination for callus differentiation was 0.9 mg/L 6⁃BA+0.6 mg/L NAA,and the differentiation rate could reach 66.67%.In this study,the best explants suitable for callus induction of Cyperus esculentus were screened,and the conditions of callus induction,proliferation and differentiation of Cyperus esculentus were optimized,which could lay a foundation for the subsequent research and breeding application of biotechnology such as somatic hybridization and genetic transformation of Cyperus esculentus. Reference | Related Articles | Metrics

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Identification of TCP Transcription Factors in Artemisia annua and Analysis of Their Expression Patterns under MeJA Treatment MA Qiyang, SONG Ge Journal of Henan Agricultural Sciences    2024, 53 (12): 62-74.   DOI: 10.15933/j.cnki.1004-3268.2024.12.007 Abstract （1942）      PDF （13374KB）（84）       Knowledge map    Save Based on the genome data of Artemisia annua，the TCP gene family members were identified，and their protein physicochemical properties，evolutionary relationships，and cis‑acting elements were analyzed. Transcriptome data were used to analyze the expression pattern of TCP transcription factors in Artemisia annua under different light treatments，and qRT‑PCR was used to detect their expression under methyl jasmonate（MeJA）treatment.The results showed that a total of 29 TCP genes（AaTCPs）were identified. AaTCPs differed greatly in protein physicochemical properties，in which the number of amino acids and protein molecular mass ranged from 132 to 443 and 14.60 to 47.86 ku，respectively，and the isoelectric points，instability indices，and fat coefficients were in the ranges of 5.67 to 9.99，33.28 to 69.77，and 51.70 to 77.40，respectively，and all of them were nuclear proteins.Phylogenetic relationships showed that AaTCPs could be classified into class Ⅰ（11）and class Ⅱ（18），unevenly distributed on eight chromosomes.The Motif number of AaTCPs ranged from 1 to 7，all of them contained one conserved TCP2 structural domain，and a large number of light‑，hormone‑，and stress‑responsive elements were present in their promoters.Protein interaction predictions revealed tight interactions among the protein members of AaTCPs except for AaTCP12 and AaTCP8.Transcriptome data showed that the expression levels of AaTCPs varied under different light treatments，and the expression of most genes was induced by light. qRT‑PCR analysis revealed that the expression of AaTCPs all increased to different degrees under MeJA treatment，showing dynamic changes with the extension of treatment time.In summary，the AaTCP gene family plays a significant role in light regulation and hormone response processes. Reference | Related Articles | Metrics

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Lightweight YOLO v5s Blueberry Detection Algorithm Based on Attention Mechanism LIU Yongmin, ZHANG Wei, MA Haizhi, LIU Yuan, ZHANG Yi Journal of Henan Agricultural Sciences    2024, 53 (3): 151-157.   DOI: 10.15933/j.cnki.1004-3268.2024.03.016 Abstract （1931）            Knowledge map    Save To achieve precise and rapid detection of blueberries in natural environments，an improved algorithm combining lightweight networks and attention mechanisms was proposed based on YOLO v5s.Firstly，the structure of the maximum object detection layer was removed at the positions of the backbone network and detection heads，thereby reducing the number of model parameters and enhancing the model’s ability to detect small targets. Secondly，MHSA（Multi‐head self‐attention）was used to replace the C3 module before SPPF（Spatial pyramid pooling‐fast），enabling the model to learn more comprehensive feature representations and enhancing its understanding of complex spatial relationships and contextual information in blueberry images. Finally，S‐PSA（Sequential polarized self‐attention）was added to the C3 module to better capture the contextual dependencies between adjacent regions in the feature map. The experimental results showed that the improved YOLO v5s algorithm improved the detection accuracy of mature blueberries，semi mature blueberries，and immature blueberries by 1.2，4.4，2.6 percentage points，respectively，with average accuracy increase of 2.7 percentage points and 76% reduction in model parameter count. Compared with the current mainstream lightweight object detection models，the improved model has superior performance and can provide an effective solution for the visual system of blueberry picking robots in natural environments. Reference | Related Articles | Metrics

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Genome‐Wide Association Study and Candidate Gene Prediction of Arsenic Content in Wheat Grains SHI Xia, SUN Dongling, CHANG Yang, LI Wenxu, DAI Ziju, ZHOU Zhengfu, WU Zhengqing, LEI Zhensheng Journal of Henan Agricultural Sciences    2025, 54 (10): 21-32.   DOI: 10.15933/j.cnki.1004-3268.2025.10.003 Abstract （1921）      PDF （11294KB）（61）       Knowledge map    Save In order to provide a theoretical basis for the arsenic（As） biofortification breeding in wheat grains，an association population comprising 207 wheat varieties（lines）was used as material，genotyping was done by wheat 660K SNP array，combining with phenotypic data under different environments and best linear unbiased prediction（BLUP）value，a genome‐wide association study（GWAS）was conducted on As content in wheat grains，genetic loci were identified，and candidate genes associated with As content in wheat grains were predicted. The haplotype analysis and linear regression analysis methods were used to analyze the superior and inferior haplotypes of stable SNP loci associated with As content in wheat grains and their aggregated effect. The results showed that there were obvious variation in grain As content under multiple environments，and the grain As content exhibited a normal distribution.By using a mixed linear model（MLM），a total of 67 SNPs were identified to be significantly associated with grain As content at the genome‐wide level. Among these，six SNPs located on chromosomes 1A，2A，2D，3B，and 6B（AX‐110393422，AX‐110370420，AX‐109339465，AX‐109455432，AX‐109359598，AX‐111655266）were consistently detected under multiple environments，and were considered as stable quantitative trait nucleotides（QTNs）.Additionally，a SNP hotspot was identified at the end of chromosome 6B.The peak SNP AX‐111655266 was detected under all environments，which exhibited the strongest association with grain As content，explained the phenotypic variation of 15.14%—21.53% ，and was a major‐effect QTN.Based on public wheat expression profile data and differential expression analysis of candidate genes at 20 days post‐anthesis in the association population，combined with physical location and functional annotation information，candidate genes were predicted.The candidate gene TraesCS6B02G418600，tightly linked to SNP AX‐111655266，encoding a phosphate transporter PHO1‐like protein，which may be involved in transmembrane transport and signal transduction of As，was a major‐effect gene controlling grain As content in wheat. The candidate genes for the other five SNP loci were TraesCS1A02G346700，TraesCS2A02G106800，TraesCS2D02G171500，TraesCS3B02G131500，and TraesCS3B02G252500，encoding F‐box protein，thiamine pyrophosphokinase 1，GEM‐like protein 1，ubiquitin‐onjugating enzyme E2‐like protein，and protein phosphatase 2C family protein，respectively. They were candidate genes involved in regulating As accumulation in wheat grains.The grain As content in the superior haplotype group was significantly lower than that in the inferior haplotype group under all environments.Furthermore，as the number of superior haplotypes increased，the grain As content gradually decreased，demonstrating a clear additive effect. Table and Figures | Reference | Related Articles | Metrics

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Effects of Soybean and Maize Stubbles on Bacterial Community Structure and Function in Winter Wheat Rhizosphere Soil GUO Hui, LIAN Yanhao, ZHAO Zhibo, REN Yongzhe, WANG Zhiqiang, LIN Tongbao Journal of Henan Agricultural Sciences    2024, 53 (7): 79-89.   DOI: 10.15933/j.cnki.1004-3268.2024.07.009 Abstract （1914）      PDF （3686KB）（609）       Knowledge map    Save To explore the differences in the effects of maize stubble and soybean stubble in the Huang‑Huai‑Hai area，in 2023，rhizosphere soil samples at the jointing stage of winter wheat were collected under the conditions of long‑term positioning test fields for up to 4 years（2019—2023）.The responses of rhizosphere soil bacterial community structure and function to no nitrogen application（N0）and conventional nitrogen application（N240，pure nitrogen 240 kg/ha） were studied by using 16S high‑throughput sequencing technology combined with Tax4Fun2 function prediction analysis in the jointing stage of winter wheat under maize（M）stubble and soybean（S）stubble，respectively.There were four treatments（MN0，SN0，MN240，SN240）in total.The results showed that，at the phylum level，Proteobacteria，Acidobacteria，Bacteroidetes and Actinobacteriota were the dominant rhizosphere bacteria of winter wheat with a relative abundance of 66.66%—69.56%.Under N0 nitrogen application rate，the relative abundance of Bacteroidetes in S stubble was significantly increased by 19.10% compared with that in M stubble（P<0.05），and under N240 nitrogen application rate，the relative abundance of Proteobacteria in S stubble was significantly increased by 11.90% compared with that in M stubble（P<0.05）.At the order level，Vicinamibacterales and Burkholderiales were the dominant bacteria with a relative abundance of 18.42%—19.68%. Under N0 nitrogen application rate，the relative abundance of Vicinamibacterales in S stubble was significantly reduced by 13.69% compared with that in M stubble（P<0.05）；The relative abundance of Burkholderiales was increased significantly by 11.31%（P<0.05）.Through the Tax4Fun2 function prediction and the KEGG data comparison，it was found that there were significant differences in the relative abundance of seven functional pathways under the metabolism category under N0 nitrogen application rate（P<0.05），and correlation analysis further revealed that there was a significant correlation between Vicinamibacterales and Burkholderiales and amino acid metabolism pathways（P<0.01）.There were 26 functional genes involved in soil nitrogen cycling pathway in winter wheat soil bacteria，and the relative abundance of denitrification functional genes accounted for the largest proportion.Under N0 nitrogen application rate，the relative abundance of denitrification functional genes norB，norC and nirS in M stubble was significantly higher than that in S stubble（P<0.05）.In conclusion，the stubble causes the soil bacterial community structure of winter wheat to change under different amount of nitrogen. Different stubble affects the nitrogen metabolism function of bacterial community by changing the structure of rhizosphere soil bacterial community. Reference | Related Articles | Metrics

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Growth Response and Tolerance Evaluation of 32 Spinach Varieties to Cadmium Stress MIAO Mingjun, LI Jiajia, LEI Xiaokui, LIN Yang, LIAO Jichao, ZHONG Jian, LI Ju, LI Yuejian, CHANG Wei Journal of Henan Agricultural Sciences    2024, 53 (9): 108-116.   DOI: 10.15933/j.cnki.1004-3268.2024.09.011 Abstract （1903）      PDF （2257KB）（356）       Knowledge map    Save Cadmium(Cd)is a highly toxic heavy metal element.To identify Cd‑tolerant spinach varieties and screen related identification indexes,and provide a theoretical basis for the selection and breeding of Cd‑tolerant varieties,in the present study,we conducted a hydroponic experiment using 32 spinach varieties in China.The spinach was exposed to Cd stress(Cd concentration of 50 μmol/L)for 20 days,while the other group of plants was treated as a control(Cd concentration of 0 μmol/L).After the plants reached maturity,we measured the agronomic traits and Cd content.The integrated Cd tolerance of each spinach variety was evaluated by principal component analysis,affiliation function analysis,and correlation analysis.The results showed that plant height,root length,underground fresh weight,aboveground fresh weight,aboveground Cd content,underground Cd content and translocation coefficient of the participating varieties under Cd stress conditions were significantly different from the control.Under Cd stress,the growth and biomass accumulation of spinach were inhibited,and the indicators of each trait decreased significantly.The order of average reduction of each agronomic index was aboveground fresh weight>plant height>underground fresh weight>root length.The principal component analysis of Cd stress tolerance index of the seven indexes was performed,and three principal components were selected，with a cumulative variance contribution of 81.552%;The composite evaluation value(D value)showed highly significant positive correlations(P<0.01)with plant height,underground fresh weight,aboveground fresh weight,aboveground Cd content,and translocation coefficient,which can be used as the preferred indexes for the evaluation of Cd tolerance in spinach.Based on the principal component analysis,we classified the 32 spinach varieties into three categories:Cd‑tolerant,moderate,and Cd‑sensitive varieties.Among the varieties tested,Jin’ge and Micro orchid exhibited strong Cd tolerance,while Desai 176 was found to be Cd‑sensitive. Reference | Related Articles | Metrics

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A Maturity Detection Method for Hemerocallis citrina Baroni Based on Improved YOLOv5 SHENG Bin Journal of Henan Agricultural Sciences    2024, 53 (8): 145-153.   DOI: 10.15933/j.cnki.1004-3268.2024.08.016 Abstract （1883）      PDF （3862KB）（985）       Knowledge map    Save To unify identification standards and improve the detection accuracy and real‑time performance of mature Hemerocallis citrina Baroni picking，an improved GCS‑BI YOLOv5 image detection algorithm was proposed.Firstly，the Ghost lightweight neural networks were utilized to streamline the model structure and save computational resources.Secondly，in order to pay attention to the image channel information and position information simultaneously，efficient attention mechanisms，namely convolutional block attention module（CBAM）and squeeze‑and‑excitation（SE），were cross‑introduced to improve the image feature perception ability and model convergence speed.Then，a weighted bi‑directional feature pyramid network（BI FPN）was used to fuse the multi‑scale image information and improve the comprehensive detection performance of the model for different targets.The experimental results showed that compared with the original algorithm，the lightweight metrics such as the model volume，network layers，number of parameters，and floating‑point operation of the improved algorithm were reduced by 62.89%，33.12%，63.01%，68.39%，respectively.The performance metrics such as detection accuracy and recall rate were improved by 7.77，6.28 percentage points，respectively.Real‑time detection performance was improved by 33.81 f/s.It can be seen that the improved algorithm has better comprehensive performance and can meet the requirements of Hemerocallis citrina Baroni maturity detection. Related Articles | Metrics

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Analysis on Chloroplast Genome of Paederia scandens WU Minhua, WU Zijian, YE Xiaoxia, TAN Jingyi, WANG Shen, HUANG Qionglin Journal of Henan Agricultural Sciences    2024, 53 (1): 70-77.   DOI: 10.15933/j.cnki.1004-3268.2024.01.008 Abstract （1861）      PDF （6478KB）（776）       Knowledge map    Save To elucidate the structure and sequence characteristics of chloroplast genome from Paederia scandens，the chloroplast genome sequencing was performed using high‑throughput sequencing technology，and then acquired sequence was assembled，annotated and analyzed via bioinformatics procedures.The results demonstrated that the chloroplast genome of P.scandens was a circular quadripartite molecule with a length of 153 456 bp and encoded 133 genes.Altogether 54 simple sequence repeats were detected in the plastome and the A/T mononucleotide was main type.A total of 26 983 codons were searched out and those with end of A/T were frequently used.Sequence alignment demonstrated more divergence in non‑coding region among five Rubiaceae species including P.scandens.Phylogenetic tree revealed close relationship of various chloroplast genome sequences of P.scandens and clearly displayed the evolutionary position of P.scandens in Rubiaceae. Reference | Related Articles | Metrics

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Comprehensive Evaluation of Nutritional Quality of Foxtail Millet Based on Principal Component Analysis and Membership Function Method ZHANG Aiqin, GUO Bin, LIU Lilong, LI Xuhua, WANG Qing, XU Rui, ZHANG Huan Journal of Henan Agricultural Sciences    2025, 54 (1): 28-39.   DOI: 10.15933/j.cnki.1004-3268.2025.01.004 Abstract （1818）      PDF （4001KB）（199）       Knowledge map    Save In order to explore the characteristics of nutritional content of different foxtail millet varieties from Longzhong area in Gansu Province，establish a standard evaluation system of nutritional quality and screen the foxtail millet varieties with excellent nutritional quality，10 foxtail millet varieties from Longzhong area(Huining County，Baiyin City)in Gansu Province were used as materials，19 nutritional quality indexes including basic nutrients(moisture，ash，carbohydrate，protein，crude fat，starch，amylose，amylopectin contents and amylose/amylopectin)，functional nutrients(flavone，total phenol，crude polysaccharide contents)and mineral elements(Zn，Mn，Cu，Fe，Ca，Mg，Se contents)were measured，and principal component analysis and membership function methods were used to evaluate the nutritional quality of foxtail millet. The results showed that there was significant difference in the contents of 18 nutritional components excluding moisture content from 10 foxtail millet varieties，with variation coefficients ranging from 1.58% to 103.46%，which indicated that there was great difference in the individual nutritional quality index among different foxtail millet varieties. Protein，amylose，polysaccharide，Mg，Se and Zn contents were determined as core indexes for the evaluation of nutritional quality of foxtail millet by principal component analysis，correlation analysis and cluster analysis. The nutritional quality evaluation model was established by principal component analysis，and the top three foxtail millet varieties were M‑8(Longgu 23)，M‑10(Longgu 029)and M‑9(Longgu 032)based on the comprehensive score of nutritional quality，consistent with the results of the membership function evaluation，which indicated that these three varieties had better nutritional quality.Ten foxtail millet varieties could be divided into three categories by cluster analysis，and class Ⅱ included M‑8 and M‑9，whose comprehensive nutritional quality performed better. Table and Figures | Reference | Related Articles | Metrics

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Comparative Analysis of Chromosome Sets Characteristics of Sesame Cultivated Species and Wild Species Based on FISH and GISH Technologies MA Qin, ZHAO Ruihong, JU Ming, CHEN Chengbin, DUAN Yinghui, YANG Weifei, MIAO Hongmei, ZHANG Haiyang, Journal of Henan Agricultural Sciences    2024, 53 (10): 48-53.   DOI: 10.15933/j.cnki.1004-3268.2024.10.006 Abstract （1806）      PDF （2483KB）（383）       Knowledge map    Save To reveal the evolutionary characteristics of Sesamum,explore the genome structure revolution and species evolution,and promote the development and utilization of wild resources,the cultivated species S.indicum(var.Yuzhi 11)and the 2n=26 type wild species S.alatum(var.3651)were used as experimental materials,fluorescence in situ hybridization(FISH)and genome fluorescence in situ hybridization(GISH)techniques were used to analyze the chromosome sets characteristics of sesame cultivated species and wild species.The results showed that both the cultivated species Yuzhi 11 and the wild species S.alatum(var.3651)were 2n=2x=26 karyotype;the rDNA⁃FISH hybridization results indicated that in the 13 pairs of chromosomes of the cultivated species Yuzhi 11,3 pairs of chromosomes(the 7th,8th and 9th pairs)had 45S rDNA⁃specific signals at the short arm tips,which were displayed as satellite specific chromosome.At the same time,2 pairs of chromosomes(the 5th and 11th pairs)had 5S rDNA⁃specific signals on the short arms,with the 5S rDNA and 45S rDNA signals located on different chromosomes. In the wild species S.alatum(var.3651),2 pairs of chromosomes(the 4th and 7th pairs)carried 45S rDNA hybridization signals and 1 pair of chromosome(the 4th pair)carried 5S rDNA specific signals,with the 5S rDNA and 45S rDNA signals located on the same chromosome but at different positions.This indicated that there were significant differences in chromosome characteristics between the cultivated species and the wild species S.alatum(var.3651).The GISH hybridization results showed that when using the genomic DNA of the cultivated species Yuzhi 11 and the wild species S.alatum(var.3651)as probes for self⁃hybridization,each chromosome carried hybridization signals of varying strengths,while hybridization with the other’s chromosomes showed very few hybridization signals.The cultivated species and the wild species S.alatum(var.3651)have the same number of chromosomes,but there are clear differences in the quantity,distribution of rDNA,and GISH signal sites,indicating that the 2n=26 type cultivated species and wild species have distant phylogenetic relationship. Reference | Related Articles | Metrics

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Screening and Safety Evaluation of Post‑emergence Herbicides for Controlling Broadleaf Weeds in Sesame Fields SU Wangcang, NIU Yujia, YAN Zhaoling, DU Li, XUE Fei, SUN Lanlan, XU Hongle, WU Renhai, LIU Hongyan Journal of Henan Agricultural Sciences    2024, 53 (11): 101-108.   DOI: 10.15933/j.cnki.1004-3268.2024.11.011 Abstract （1783）      PDF （1267KB）（269）       Knowledge map    Save To explore safe and efficient chemical weed control technologies for sesame after emergence，the herbicides that exhibit high safety for sesame and have good control effects on broadleaf weeds were screened through indoor potting experiments and field trials. The results of the indoor trials showed that among the 17 broadleaf herbicides，only isoproturon and bentazone were discovered to be highly safe for sesame.Futher，the safety of isoproturon and bentazone to sesame and their control efficacy against broadleaf weeds were evaluated through field trials. The results showed that the treatment of isoproturon at 420—630 g/ha（active ingredient，the same below）caused phytotoxicity in sesame，such as the emergence of drug spots and growth inhibition，etc. Nevertheless，sesame growth recovered to normal within 30 d after the treatment. Sesame treated with 336—420 g/ha bentazone had significantly higher plant height than the control treatment at 30 d after the treatment，but was significantly lower than the artificial weeding treatment. The total plant control efficacy of isoproturon at 420—630 g/ha against broadleaf weeds such as Acalypha australis L.and Clinopodium polycephalum Vaniot was 84.00%—90.40%，the total fresh weight control efficacy was 87.62%—94.30%，and the sesame seed yield increased by more than 37%.The control efficacy of bentazone at a dosage of 336—504 g/ha against Acalypha australis L.was unsatisfactory，with the total plant control efficiency being lower than 40% and the total fresh weight control efficiency being lower than 57%，and sesame yield was merely increased by 11.53%—14.72%.In conclusion，the application of isoproturon at 420—630 g/ha during the 5—6 leaves stage of sesame is safe and can be used for the control of broadleaf weeds in sesame fields，presenting significant application prospects. Reference | Related Articles | Metrics

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Identification and Analysis of Amylase Genes Related to Pre⁃harvest Sprouting Resistance in Wan 1329 Based on Transcriptome Sequencing WANG Zhen, ZHANG Bin, SHI Lichao, LIU Rui, LI Jinxiu, ZHOU Ran, YANG Ling, ZHAO Qian, LI Jinbang Journal of Henan Agricultural Sciences    2025, 54 (12): 32-39.   DOI: 10.15933/j.cnki.1004-3268.2025.12.004 Abstract （1727）      PDF （6482KB）（91）       Knowledge map    Save Using Jimai 22 with high sensitivity to pre⁃harvest sprouting（PHS）as the control，the PHS resistance and α⁃amylase activities of the new white⁃grain wheat variety Wan 1329 and its parent Lianmai 2 were analyzed. During the critical period when α⁃amylase activity changed，transcriptome sequencing，KEGG and GO enrichment analysis were employed to identify differentially expressed amylase genes，and real⁃time fluorescence quantitative PCR（qRT⁃PCR）was used for verification their expression patterns.In addition，AlphaFold was employed to analyze the effects of coding sequence variations on protein structure. The results showed that both Wan 1329 and Lianmai 2 exhibited moderate resistance to PHS，while Wan 1329 had a lower relative germination index，indicating stronger resistance. At 24 h after seed imbibition，the α⁃amylase activities of Wan 1329 and Lianmai 2 were markedly lower than that of Jimai 22，and α⁃amylase activities at 20 h，24 h，and 72 h were highly positively correlated with the relative germination index. Transcriptome sequencing，KEGG and GO enrichment analysis revealed that two α⁃amylase genes located on the long arm of chromosome 6D， TraesCS6D02G313300 and TraesCS6D02G313500，were candidate α⁃amylase genes. The third exon of TraesCS6D02G313500 in Wan 1329 was elongated by 138 bp，extending into the 3´UTR region.qRT⁃PCR validation confirmed that the expression patterns of both genes were consistent with transcriptome analysis，and the cumulative relative expression level of TraesCS6D02G313500 in Wan 1329 was significantly lower than that in Lianmai 2.AlphaFold prediction suggested that this variation caused a C⁃terminal extension of α⁃amylase，resulting in decreased structural stability and increased conformational flexibility.Collectively，TraesCS6D02G313300 and TraesCS6D02G313500 are key genes related to PHS resistance in Wan 1329，among which the structural variation of TraesCS6D02G313500 may further enhance resistance to PHS. Table and Figures | Reference | Related Articles | Metrics

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Identification and Expression Analysis of HD‑ZIP Family in Salvia miltiorrhiza LI Wan, CHENG Ruixing, DANG Xin Journal of Henan Agricultural Sciences    2024, 53 (7): 66-78.   DOI: 10.15933/j.cnki.1004-3268.2024.07.008 Abstract （1718）      PDF （6842KB）（262）       Knowledge map    Save To screen HD‑ZIP genes（SmHD‑ZIPs）in Salvia miltiorrhiza in response to high temperature stress，SmHD‑ZIPs were identified and analyzed by bioinformatics，and the expression pattern of SmHD‑ZIPs under high temperature stress was detected by qPCR. The results showed that there were 44 HD‑ZIP genes in S.miltiorrhiza，most of which were unevenly distributed on 8 chromosomes. SmHD‑ZIPs could be divided into four subfamilies：HD‑ZIP Ⅰ，HD‑ZIP Ⅱ，HD‑ZIP Ⅲ，HD‑ZIP Ⅳ. The sequence analysis results showed that the amino acid residues of these proteins ranged from 180 to 982，and the relative molecular weight of these proteins ranged from 20. 947 ku to 109. 620 ku. SmHD‑ZIPs were hydrophilic proteins without transmembrane domain，and most of them did not contain signal peptides.The isoelectric points of SmHD‑ZIPs were between 4. 48 and 10. 91，and they were almost expressed in the nucleus with the poor protein stability. Among the 44 SmHD‑ZIP genes，there were 10 gene duplication events，all of which were purified selection. The results of structure and motif analysis showed that the exon number of members of the same subfamily was basically the same，and the members of HD‑ZIP Ⅲ subfamily had the largest exon number and the largest motif number. Motifs 1 and 6 were conserved motifs of SmHD‑ZIP，motifs 10，11，12，13 and 15 was unique to the HD‑ZIP Ⅲ subfamily，while motif 5 were unique to the HD‑ZIP IV subfamily. By analyzing the expression patterns of SmHD‑ZIPs under high temperature（37℃）stress，it was found that the number of genes with increased and decreased expression was basically the same. Among the genes with up‑regulated expression，the expression levels of SmHD‑ZIP1.11，SmHD‑ZIP1.13，SmHD‑ZIP2.2，SmHD‑ZIP2.5，SmHD‑ZIP3.1，SmHD‑ZIP3.4，SmHD‑ZIP4.9，SmHD‑ZIP4.10 and SmHD‑ZIP4.12 were increased by more than 10 times，which could be used as candidate gene resources to improve the heat resistance of S.miltiorrhiza. Reference | Related Articles | Metrics

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Effects of Phosphorus Fertilizer Application Depth on Root Distribution，Nitrogen Uptake and Utilization and Yield of Foxtail Millet ZHU Cancan, FU Senjie, QIN Na, WANG Chunyi, DAI Shutao, SONG Yinghui, WEI Xin, LI Junxia Journal of Henan Agricultural Sciences    2023, 52 (12): 22-30.   DOI: 10.15933/j.cnki.1004-3268.2023.12.003 Abstract （1693）      PDF （1455KB）（794）       Knowledge map    Save In order to provide a theoretical basis for the efficient utilization of phosphate fertilizer in foxtail millet production，a field experiment was conducted with four phosphate fertilizer application depths of 5 cm（P5），10 cm（P10），15 cm（P15）and 20 cm（P20）with no phosphate fertilizer application as control（CK），and the effects of phosphorus fertilizer application depth on root distribution，dry matter accumulation and transportation，nitrogen uptake and utilization，and yield of foxtail millet were studied.The results showed that suitable application depth of phosphate fertilizer promoted the growth of foxtail millet roots，and increased root length，root surface area and dry weight of root，showing a trend of P15>P10>P5>P20>CK. With the increase of phosphate fertilizer application depth，the root length，root surface area and dry weight of root of foxtail millet in deep soil increased，and the values showed P20>P15>P10>P5>CK in 20—40 cm soil layer. Suitable application depth of phosphate fertilizer could promote dry matter accumulation and transportation of foxtail millet. Compared with P5 treatment，the dry matter accumulations at maturity of P10，P15 and P20 treatments increased by 4.60%，10.29% and 0.92%，respectively，and the dry matter transfer rates increased by 6.49%，10.06% and -20.13%，respectively.Deep application of phosphate fertilizer increased the uptake and utilization of nitrogen by roots in deep soil.The available nitrogen content in deep soil，especially in the 20—40 cm soil layer，significantly decreased compared with P5 treatment. Compared with P5 treatment，the total nitrogen accumulations of P10，P15 and P20 treatments increased by 3.91%，17.85% and 5.31% at maturity，respectively；nitrogen absorption efficiencies increased by 5.88%，19.12% and 7.35%，and partial productivities of nitrogen increased by 18.95%，32.88% and -1.26%，respectively. Suitable application depth of phosphate fertilizer increased grain weight per spike，yield and harvest index，which all showed P15>P10>P5>P20>CK，the yields of P10 and P15 treatments increased by 18.98% and 32.86% compared with P5 treatment，respectively，and the yield of P20 treatment decreased by 1.27% compared with P5 treatment.In conclusion，15 cm is the optimal depth of phosphorus fertilizer application，which can promote the growth and down lapping of foxtail millet roots，improve the uptake and utilization of nitrogen by roots in soil，promote the accumulation and transportation of dry matter，and increase the yield by 32.86% compared with the shallow application of phosphorus fertilizer at 5 cm. Reference | Related Articles | Metrics

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Comprehensive Evaluation of Drought Tolerance and Physiological Regulatory Mechanism of Wheat Seedlings Liu Yuhang, Shi Yuhan, Yang Yiting, Li Xueting, Jiang Mengxin, Li Jiayi, Li Junhua, Sang Shifei, Feng Liuchun, Wang Li Journal of Henan Agricultural Sciences    2026, 55 (7): 1-10.   DOI: 10.15933/j.cnki.1004-3268.2026.07.001 Abstract （1673）      PDF （31619KB）（85）       Knowledge map    Save With 120 wheat germplasms as materials，PEG6000 solution was used to simulate drought stress at seedling stage.Plant height，root length，fresh weight of shoot，dry weight of shoot，fresh weight of root，dry weight of root，root⁃shoot ratio and SPAD value were determined，and their drought tolerance coefficients were calculated.Principal component analysis（PCA）was performed based on their drought tolerance coefficients，cluster analysis was conducted using the comprehensive drought tolerance value（D），and comprehensive evaluation of drought tolerance of wheat germplasms was carried out.Highly drought⁃tolerant and drought⁃sensitive germplasms were selected to determine H2O2，malondialdehyde（MDA），proline（Pro），soluble sugar（SS）contents and catalase（CAT）activity，so as to analyze the physiological regulatory mechanism of drought tolerance.The pre⁃experiment confirmed that 25% was the optimal PEG6000 concentration，all phenotypic traits showed extremely significant difference compared with the control under this concentration. Drought stress obviously decreased fresh weight of shoot，dry weight of shoot，and SPAD value，but increased root length and root⁃shoot ratio.The correlations among phenotypic traits changed obviously under drought stress compared with those under normal water condition. Under drought stress，the correlation between root length and dry weight of shoot disappeared，SPAD value was significantly positively correlated with plant height and fresh weight.Genotype，drought and their interaction had extremely significant effects on all eight phenotypic traits.PCA showed that the first four principal components accounted for 86.67% of the total variation.The drought tolerance coefficients of all traits were extremely significantly positively correlated with D value，among which the correlation coefficients between drought tolerance coefficients of fresh weight of shoot，dry weight of shoot，fresh weight of root，dry weight of root and D value were higher， which were core indexes for drought tolerance identification. Cluster analysis based on D value showed that the wheat germplasms were divided into five groups，and 15 highly drought⁃tolerant germplasms were screened out，including Xinong 109，Luohan 22，Shannong 116，Xinmai 26，Yu’an X208，Bainong 207，Luyuan 502，Bainong 4199，Xinmai 60，Xinong 519，Wenmai 801，Saidemai 8，Yunong 186，Xinong 20，and Fengdecun 20.Compared with highly drought⁃sensitive germplasms， highly drought⁃tolerant germplasms had significantly lower H2O2 and MDA contents，and significantly higher CAT activity，Pro and SS contents，and CAT activity，Pro and SS contents were significantly or extremely significantly positively correlated with D value，indicating that highly drought⁃tolerant germplasms alleviate drought damage by enhancing CAT activity，accumulating Pro and SS，and reducing H₂O₂ and MDA accumulation. Table and Figures | Reference | Related Articles | Metrics

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Research Progress of Mineral Modified Biochar in Soil Carbon Sequestration Application Ye Jiangnan, Chen Min, Sun Yuan, Yang Jiali, Wang Wenshuo, Han Zhengdong, Lu Shengyong Journal of Henan Agricultural Sciences    2026, 55 (9): 1-7.   DOI: 10.15933/j.cnki.1004-3268.2026.09.001 Abstract （1670）      PDF （1276KB）（71）       Knowledge map    Save The preparation methods of mineral modified biochar were reviewed，and the influence and mechanism of mineral modification on the stability of biochar were summarized.At the same time，taking the soil carbon sequestration process mediated by mineral modified biochar as the main line，the effect and mechanism of mineral modified biochar in soil carbon sequestration application were discussed，and the key scientific problems to be further explored in the application of mineral modified biochar in soil carbon sequestration were prospected，in order to provide reference for the development of efficient and lasting carbon sequestration biochar materials. Reference | Related Articles | Metrics

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Identification of HSF Gene Family in Eucommia ulmoides and Its Expression Analysis under Adverse Stresses WU Shujuan, XU Feng, WANG Guangan, WANG Chen Journal of Henan Agricultural Sciences    2024, 53 (9): 46-56.   DOI: 10.15933/j.cnki.1004-3268.2024.09.005 Abstract （1670）      PDF （6355KB）（619）       Knowledge map    Save Heat shock transcription factors（HSFs）play important roles in plant growth and development and in response to abiotic stresses.In order to understand the information of HSF gene family members in Eucommia ulmoides and reveal the structural characteristics and expression patterns of EuHSFs,this study analyzed the physicochemical properties,protein structure,phylogeny,gene structure,conserved domains,promoter cis‑acting elements,and the expression patterns of EuHSF genes in different developmental periods and abiotic stresses by bioinformatics methods and qRT‑PCR.The results showed that a total of 21 EuHSF genes were identified from Eucommia ulmoides,which differed in protein properties,with amino acid numbers,protein theoretical molecular masses,isoelectric points and instability coefficients ranging from 68—369,7.72—42.06 ku,4.31—9.22,and 7.05—67.28,respectively,and mainly consisted of acidic,hydrophilic,and unstable nuclear proteins.Phylogeneticanalysis showed that EuHSFs were divided into three subgroups,including ClassⅠ(1 EuHSF),ClassⅡ(7 EuHSFs)and ClassⅢ(13 EuHSFs),and promoter cis‑acting element analysis revealed that a large number of light‑responsive and hormone‑responsive elements in EuHSF genes.In addition,EuHSF genes were expressed at different developmental periods in Eucommia ulmoides leaves,but there were significant differences in the expression patterns. qRT‑PCR revealed that all EuHSFs responded to different abiotic stresses(high temperature,low temperature,high salinity,and drought),e.g.,most of the EuHSFs showed elevated expression under high‑temperature stress with the prolongation of the treatment time，and they were strongly responsive to both high and low temperatures.In summary,the EuHSF gene family has the function of regulating plant response to abiotic stresses. Reference | Related Articles | Metrics

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Effect of Nitrogen and Zinc Application on Filling Characteristics of Grains at Different Positions of Maize Ear ZHANG Panpan, LI Chuan, ZHANG Meiwei, YU Xiaohong, MU Weilin, GUO Hanxiao, QIAO Jiangfang, ZHANG Guitang Journal of Henan Agricultural Sciences    2024, 53 (9): 16-27.   DOI: 10.15933/j.cnki.1004-3268.2024.09.002 Abstract （1652）      PDF （1837KB）（226）       Knowledge map    Save The high Zn efficiency variety Zhengdan 958(ZD958)and Zn no‑sensitive variety Longping 638(LP638)were used as materials.The field plot experiment was conducted with three N rates[225 kg/ha(HN),180 kg/ha(MN)and 90 kg/ha(LN)]and two foliar Zn treatments［no Zn(F0),spraying Zn of 1∶1 at jointing stage and flare opening stage(F1)],and the effects of N and Zn application on the filling characteristics of grains at different positions and their relationship with grain yield of different Zn efficiency varieties were studied,so as to provide reference for N and Zn application in the maize production.The results showed that HN treatment had the highest yield,followed by MN treatment,and there was no significant difference between HN treatment and MN treatment.HN treatment increased grain weight at different positions.MN treatment delayed the time with the maximum grain‑filling rate(TGmax)at different positions,and increased the maximum grain‑filling rate(Gmax)at upper position and active grain‑filling period(D)at basal position of ZD958 ear,while HN treatment delayed TGmax,prolonged D of different positions,and enhanced the grain weight with the maximum grain‑filling rate(WGmax)at upper and basal positions of LP638 ear,thus increasing grain yield.Compared with F0 treatment,F1 treatment significantly decreased the bare tip length by 9.9%,significantly increased the yield by 2.0%,increased the grain weight,extended D and postponed TGmax at upper and basal positions of ZD958 ear,and increased WGmax.The grain yield was significantly positively correlated with TGmax at basal position of ear,and the grain weight at middle position of ear was extremely significantly positively correlated with the grain weight at basal position of ear.To sum up,applying 180 kg/ha N and spraying 4.5 kg/ha Zn of 1∶1 at jointing stage and flare opening stage can delay TGmax at upper and basal positions of ear，extend D,and increase WGmax and grain weight of high Zn efficiency maize genotype,thus promoting the increase of yield. Reference | Related Articles | Metrics

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Fine‑Grained Flower Image Classification Based on Neural Network Architecture Search ZHENG Xingkai, YANG Tiejun, HUANG Lin Journal of Henan Agricultural Sciences    2024, 53 (5): 164-171.   DOI: 10.15933/j.cnki.1004-3268.2024.05.018 Abstract （1633）      PDF （3807KB）（370）       Knowledge map    Save To enhance the automation of deep convolutional neural network(CNN)design and improve fine‑grained flower image classification accuracy，an advanced neural network search approach based on differentiable architecture search(DARTS) was proposed.This method automatically constructed fine‑grained flower image classification models.Initially,an attention‑convolution module was constructed to create a comprehensive attention‑convolution search space，thereby increasing the network’s focus on discriminative features. Subsequently,a densely connected reduction cell(DCR cell)with more shallow feature input nodes was developed to retain additional shallow feature information，reducing the loss of discriminative feature information and promoting multi‑scale feature fusion.Lastly,the positions of DCR cells were adjusted when stacking the best cells to create network models of varying parameter sizes,enabling deployment on a broader range of terminal devices.The results showed that this method took approximately 4.5 hours to find the optimal neural network model,achieving classification accuracies of 96.14% on the Oxford 102 dataset and 94.12% on the Flower 17 dataset.Compared with methods like AGNAS,it improved accuracy by 1.40 percentage points on Oxford 102 and 3.09 percentage points on Flower 17. Reference | Related Articles | Metrics

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Identification and Agronomic Trait Analysis of Pre⁃harvest Sprouting⁃Resistant Mutants of Zhoumai 22 WANG Yubo, WANG Yixing, DING Lin, XU Hao, ZHAO Wei, LI Na, JIE Yize, TIAN Zhicheng, MENG Panpan, TANG Jianwei, DONG Chunhao, LI Qiaoyun, YIN Guihong, HUANG Zhenpu Journal of Henan Agricultural Sciences    2026, 55 (6): 11-22.   DOI: 10.15933/j.cnki.1004-3268.2026.06.002 Abstract （1555）      PDF （14737KB）（46）       Knowledge map    Save Aiming at the serious shortage of pre⁃harvest sprouting（PHS）⁃resistant germplasm and cultivars in the Huang⁃Huai wheat region，a mutant library was constructed using Zhoumai 22 by ethyl methanesulfonate（EMS） mutagenesis.PHS⁃resistant mutants with excellent agronomic traits were screened through PHS resistance identification and agronomic trait analysis，so as to provide elite germplasm resources for PHS resistance breeding. The results showed that a total of 36 mutants with moderate or higher PHS resistance were identified from 1 189 mutants，which exhibited large variation coefficients and abundant variations in plant height，effective tiller number per plant，spike length，grain number per spike，1 000⁃grain weight，and grain yield per plant.Among the 36 PHS⁃resistant mutants，23 mutants showed no significant difference in plant height compared with wild type（WT），16 mutants had no significantly lower grain yield per plant than WT，and eight mutants had no significantly lower 1 000⁃grain weight than WT. Twelve mutants（SFY04，SFY05，SFY06，SFY13，SFY15，SFY18，SFY21，SFY22，SFY23，SFY24，SFY27，and SFY33）performed well in both plant height and grain yield per plant. Among them，SFY05，SFY13，SFY18，SFY22，SFY24，and SFY27 showed moderate or higher PHS resistance in two cropping years.Among the 16 mutants with no significantly lower grain yield per plant than WT，two mutants（SFY30 and SFY04）had no significant difference in 1 000⁃grain weight compared with WT，retaining the large⁃grain characteristic of WT.Among them，SFY30 was a dwarf mutant with large grains. Grain yield per plant was extremely significantly positively correlated with effective tiller number per plant.The 36 PHS⁃resistant mutants were clustered into four groups.Group Ⅰ contained 12 mutants including SFY03 and so on，with relatively balanced comprehensive traits. Group Ⅱ contained 20 mutants including Zhoumai 22 and so on，with obvious high⁃yield characteristics. Group Ⅲ contained four mutants including SFY15 and so on，belonging to the grain⁃number⁃per⁃spike superior type. Group Ⅳ had only one mutant（SFY19），which was extremely prominent in plant height and spike length（both the highest），belonging to a special ecotype or genetic extreme material. In conclusion，13 PHS⁃resistant mutants with excellent agronomic traits were developed and identified，including 12 mutants with superior performance in both plant height and grain yield per plant，and one dwarf mutant with large grains.Among them，six mutants（SFY05，SFY13，SFY18，SFY22，SFY24，and SFY27）possessed stable moderate or higher PHS resistance. Table and Figures | Reference | Related Articles | Metrics

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Comprehensive Evaluation of Photosynthetic Performance of Foxtail Millet Varieties and Screening of High Photosynthetic Efficiency Varieties YAN Feng, DONG Yang, ZHAO Fuyang, HOU Xiaomin, LI Qingquan, WANG Lida, LI Qingchao, LIU Yang, ZHANG Lei, LIU Kai Journal of Henan Agricultural Sciences    2024, 53 (5): 40-48.   DOI: 10.15933/j.cnki.1004-3268.2024.05.005 Abstract （1553）      PDF （1323KB）（560）       Knowledge map    Save A field experiment was conducted to determine the photosynthetic indexes[intercellular CO2 concentration(X1),ranspiration rate(X2),stomatal conductance(X3),net photosynthetic rate(X4),instantaneous water use efficiency(X5)]and leaf morphological indexes［leaf area(X6)，dry leaf weight(X7),specific leaf weight(X8)]of 37 foxtail millet varieties,the fuzzy membership function,principal component analysis,correlation analysis and cluster analysis were used to comprehensively evaluate the photosynthetic performance of foxtail millet varieties,the varieties with high photosynthetic efficiency were screened,and the regression equation of photosynthetic performance prediction was established by stepwise regression analysis,so as to provide a reference for the screening and utilization of high photosynthetic efficiency foxtail millet germplasm. The results showed that the photosynthetic indexes and leaf morphological indexes were significantly different among 37 foxtail millet varieties.The CV ranged from 3.41% to 33.94%.The CV of stomatal conductance was the largest(33.94%),and the CV of specific leaf weight was the smallest(3.41%).The results of correlation analysis showed that intercellular CO2 concentration was significantly negatively correlated with transpiration rate and net photosynthetic rate;Transpiration rate was significantly positively correlated with net photosynthetic rate;Instantaneous water use efficiency was significantly negatively correlated with transpiration rate and net photosynthetic rate;Leaf area was significantly positively correlated with dry leaf weight.Principal component analysis was used to transform the 8 indicators into 3 independent comprehensive indicators.Transpiration rate and net photosynthetic rate could be used as evaluation indicators for the identification of photosynthetic performance of foxtail millet.Through cluster analysis,37 foxtail millet varieties were divided into three categories(high photosynthetic efficiency，medium photosynthetic efficiency and low photosynthetic efficiency),and 8 high photosynthetic efficiency varieties,including Nenxuan 22,Datong 25,Gonggu 88,Yangu 18,Jingu 20，Mengfeng 7,Longgu 13 and Gonggu 75,were screened out,which could be used as parent materials for high photosynthetic efficiency breeding.The regression model of foxtail millet photosynthetic performance was established by stepwise regression analysis(D=-1.391 984 196+0.000 619 787 003 4X1+0.051 519 289 48X2+0.674 399 672 2X3+0.013 844 082 338X4+0.058 230 284 78X5+0.003 403 878 541X6+0.051 881 997 92X8）. Reference | Related Articles | Metrics

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Light Weight Detection Algorithm for Apple Surface Defect Based on Improved YOLOv7 LI Dahua, KONG Shu, LI Dong, YU Xiao Journal of Henan Agricultural Sciences    2024, 53 (3): 141-150.   DOI: 10.15933/j.cnki.1004-3268.2024.03.015 Abstract （1535）      PDF （4276KB）（1104）       Knowledge map    Save Aiming at how to improve the detection speed and accuracy of apple surface defects and solve the problem of large model memory ratio，a lightweight detection algorithm for apple surface defects based on improved YOLOv7 was proposed. Firstly，GhostNetV2 was introduced as the backbone of YOLOv7 network，which effectively reduced the model complexity and improved the detection speed.SimAM attention‐free mechanism was introduced to enhance the feature information of different depth.The bidirectional weighted feature pyramid BiFPN was used for weighted feature fusion to further improve the detection accuracy of apple surface defects.Finally，the ECIOU loss function was used to calculate the boundary frame loss，which further improved the convergence speed and the overall performance of the model.Experimental results showed that compared with the original YOLOv7 network，the improved model improved the apple surface defect detection mAP@0.5 by 2 percentage points，the accuracy rate and recall rate by 1.7 and 3.9 percentage points respectively. The model decreased by 20.8 MB and the speed increased by 36.43 FPS.Its comprehensive performance was also better than SSD，CenterNet and other mainstream algorithms，which can realize the rapid and accurate diagnosis of apple surface defects. Reference | Related Articles | Metrics

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Tissue Screening and Condition Optimization of Protoplast Preparation of Cyperus esculentus ZHANG Xiangge, ZHU Yajing, LU Lili, SONG Wanxian, ZHANG Xinyou, HU Haiyan, YU Meiqin, LI Juzheng, WANG Huiwei Journal of Henan Agricultural Sciences    2023, 52 (12): 49-56.   DOI: 10.15933/j.cnki.1004-3268.2023.12.006 Abstract （1524）      PDF （2734KB）（258）       Knowledge map    Save In order to obtain Cyperus esculentus protoplasts efficiently，the tissue parts including young shoots，young roots，young leaves，young leaf sheaths，tillering nodes and stolons were screened for protoplast preparation by single factor test method，and the enzymatic hydrolysis conditions such as enzyme combination dosage，enzymatic hydrolysis time，osmotic pressure stabilizer dosage and pH value of the enzyme solution were also optimized.The results showed that the best tissue part for protoplast preparation of Cyperus esculentus was young tillering node；and the optimal enzymatic hydrolysis conditions were as follows：the enzyme combination dosage was 2.0% cellulase+0.9% macerozyme，the enzymatic hydrolysis time was 6 h，the osmotic pressure stabilizer（mannitol）dosage was 11%，and the pH value of the enzyme solution was 6.0.Under these conditions，0.2 g of Cyperus esculentus tillering nodes were added to 4 mL of enzyme solution，and then the protoplast yield could reach 22.91×105 per gram and the protoplast activity could reach 90.72%.In this study，an efficient method for protoplast preparation of Cyperus esculentus was obtained，which could lay a foundation for subsequent protoplast fusion，germplasm innovation and hybrid breeding of Cyperus esculentus. Reference | Related Articles | Metrics

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The Complete Gene Sequencing of Bacilus subtilis Isolated from Rehmannia glutinosa and Study of Genes Related to Growth‑promoting Effects LA Guixiao, WANG Linlin, GUO Junqi, ZHAO Yulong, GUO Hongxia, JIA Hui, YANG Tiegang Journal of Henan Agricultural Sciences    2024, 53 (12): 75-85.   DOI: 10.15933/j.cnki.1004-3268.2024.12.008 Abstract （1522）      PDF （3510KB）（631）       Knowledge map    Save To investigate the genes associated with the growth‑promoting effects of Bacillus subtilis in Rehmannia glutinosa，whole genome sequencing was performed in this study and the genes associated with the growth‑promoting effects were discovered through functional annotation.The results showed that the genome size of Bacillus subtilis，an endophytic growth‑promoting bacterium of Rehmannia glutinosa，was 4 310 699 bp，with a GC content of 43.44%，and a total of 4 521 predicted coding genes.A total of 3 013，3 354，4 395，206，52，and 212 functional genes were annotated in the clusters of orthologous groups（COG） ，gene ontology（GO） ，kyoto encyclopedia of genes and genomes （KEGG） ，carbohydrate‑active enzymes（CAZyme），comprehensive antibiotic resistance database（CARD），and virulence factor database（VFDB），respectively.The annotation results indicated that there were 46 genes related to growth‑promoting function，with the majority of these genes involved in phosphorus solubilization，nitrogen fixation，and iron carrier processes.In the meantime，anti‑SMASH predicted that a total of 409 genes were enriched into 12 secondary metabolite synthesis gene clusters，with the majority encoding eight repressors，including fengycin，1‑carbapen‑2‑em‑3‑carboxylic acid，bacillaene，bacilysin，subtilosin A，surfactin，pulcherriminic acid，and bacillibactin.Among these，pulcherriminic acid and bacillibactin had been observed to facilitate the chelation of ferric ions in the surrounding environment. Reference | Related Articles | Metrics

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Identification of Citrus Diseases Based on Improved ShuffleNet V2 YU Yannan, MO Yongbin, YAN Jichi, XIONG Chunlin, DOU Shiqing, YANG Rongfeng Journal of Henan Agricultural Sciences    2024, 53 (1): 142-151.   DOI: 10.15933/j.cnki.1004-3268.2024.01.016 Abstract （1514）      PDF （3756KB）（324）       Knowledge map    Save Large convolutional neural networks are difficult to deploy in practical applications because of the complexity of models，while lightweight networks are often less accurate than the former because of the optimization of model structure. To solve these problems，ShuffleNet V2 was improved and a lightweight MAM‑ShuffleNet citrus disease recognition model was proposed. Firstly，the mixed attention module（MAM）was introduced in ShuffleNet V2 to improve the ability of the model to extract disease features. Secondly，Ghost module was used to optimize the convolutional layer in the network，which effectively reduced the number of network model parameters and calculation cost. Finally，the stacking times of ShuffleNet V2 unit in the network structure were adjusted to further simplify the network parameters. The results showed that the average recognition accuracy of MAM‑ShuffleNet model reached 97.7% in the self‑built citrus leaf data. Compared with the original ShuffleNet V2，the number of parameters was reduced by 45.7%，and the recognition accuracy was increased by 1.2 percentage points.The comprehensive performance was better than ResNet50 and DenseNet121 models. Reference | Related Articles | Metrics

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Screening and Breeding Utilization of Red‑Grain Wheat Germplasm Resistant to Pre‑harvest Sprouting FU Mengsi, ZUO Zhixuan, XU Pei, YUE Ling, HU Xigui, HU Tiezhu, LI Gan, RU Zhengang, ZHAO Laibin Journal of Henan Agricultural Sciences    2025, 54 (11): 30-39.   DOI: 10.15933/j.cnki.1004-3268.2025.11.004 Abstract （1451）      PDF （10832KB）（43）       Knowledge map    Save In order to screen new germplasm with resistance to pre‑harvest sprouting（PHS），discover new genes with resistance to PHS，and evaluate the breeding utilization of red‑grain wheat with resistance to PHS in the Huanghuai wheat‑growing region，field germination percentages of 98 winter wheat varieties（lines）in Huanghuai wheat‑growing region，33 wheat varieties（lines）in southwest wheat region，and 310 hybrid F1 generations with the southwestern wheat materials as the first‑generation hybrid parents and materials from the Huanghuai wheat region as the backcross parents were analyzed. Five materials were randomly selected for indoor germination percentage verification，and genes related to PHS resistance were detected in 12 materials（4 red‑grain materials with resistance to PHS and 8 white‑grain materials with susceptibleness to PHS）.The results showed that the field germination percentages of 98 wheat varieties（lines）in Huanghuai wheat‑growing region and 33 wheat varieties（lines）in southwest wheat region were grouped into four categories. Twenty‑five materials with germination percentage ranging from 0 to 11% constituted the class Ⅳ，which were resistant to PHS，and most of them were red‑grain materials（68%）. The indoor germination percentage results showed the same trend with the germination percentage result in field.Among the 195 F1 materials with red‑grain material as the first parents，114 F1 materials with germination percentage of 0—16% were clustered in class Ⅳ（resistant to PHS），accounting for 93%，which was much higher than that of the offspring with white‑grain material as the first parents（7%），indicating that red‑grain material contributed most of the PHS resistance of the hybrid offspring，and using red‑grain material could significantly improve the PHS resistance. Among the 195 F1 materials，111 F1 materials were white‑grain materials，and 33 were resistant to PHS，indicating that the grain color and PHS resistance could be regulated. Breeding practice proved that red‑grain germplasm could be used to breed new white‑grain material with resistance to PHS. TaVp‑1B and TaSdr‑B1 genes were not detected in white‑grain and red‑grain materials，and all materials had the same Tamyb10‑A，Tamyb10‑B，Dorm‑1 and TaVp‑1A genotypes，indicating that these genes may not be the source of resistance to PHS in red‑grain materials. The red‑grain material 21B2 contained the allele of PM19a with resistance to PHS，and the red‑grain materials Chinese Spring，21B2 and 21B42 with resistance to PHS had Tamyb10‑D gene，indicating that genes PM19a and TaMyb10‑D may promote the resistance of red‑grain germplasm. At the same time，a red‑grain germplasm with resistance to PHS without TaMyb10‑D gene was screened，and white‑grain materials with resistance to PHS were obtained from the hybrids，indicating that it could be used as germplasm with resistance to PHS for breeding in Huanghuai wheat‑growing region. Table and Figures | Reference | Related Articles | Metrics

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mRNA Vaccines：Research Progress and Applications in Animal Infectious Diseases ZHAI Chongkai, MAO Fuchao, TIAN Wenjing, WANG Conghui, WANG Yingxian, ZHANG Hewei Journal of Henan Agricultural Sciences    2024, 53 (5): 10-21.   DOI: 10.15933/j.cnki.1004-3268.2024.05.002 Abstract （1450）      PDF （3322KB）（1595）       Knowledge map    Save Compared to traditional approaches，RNA vaccines offer advantages such as high safety，good efficacy，and short development cycle，which makes them have unique advantages in the prevention and control of infectious diseases.Currently，mRNA vaccines have made great progress in the prevention and control of COVID‑19，influenza，Ebola，Zika and other human diseases，but the research on mRNA vaccines for animals is still not in‑depth.Therefore，the development of mRNA vaccines for animals to prevent and control infectious diseases in livestock，poultry，and wild animals has become one of the important research directions in the field of animal diseases and public health.This review comprehensively discusses the design，preparation，delivery system，application，advantages，challenges and prospects of mRNA vaccines against animal infectious diseases，to provide valuable insights for the development and research of future animal mRNA vaccines. Reference | Related Articles | Metrics

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Effect of Biochar on Rhizosphere Soil Fungal Community Structure of Flue‑cured Tobacco Varieties with Different Resistance to Black Shank Disease REN Zhichao, LI Xiang, LI Xianfeng, WU Lili, WANG Jing, PENG Zhiliang, LIU Guoshun, YIN Quanyu Journal of Henan Agricultural Sciences    2024, 53 (1): 105-115.   DOI: 10.15933/j.cnki.1004-3268.2024.01.012 Abstract （1424）      PDF （5609KB）（208）       Knowledge map    Save To explore the effect of biochar on the fungal community structure in rhizosphere soil of flue‑cured tobacco varieties with different resistance to black shank disease，and to provide theoretical basis for regulating tobacco microenvironment and comprehensively controlling black shank disease，pot experiments were conducted with different flue‑cured tobacco varieties Jingyehuang（highly susceptible variety），K326（moderately resistant variety）and NC82（highly resistant variety）as materials，and no tobacco cultivation was used as control（CK）.Two levels of biochar and no biochar were set up，with a total of eight treatments，and the effect of biochar on fungal community structure in rhizosphere soil of flue‑cured tobacco varieties with different resistance to black shank was explored by high‑throughput sequencing technique. The results showed that，after applying biochar，the incidence and disease index of black shank disease of flue‑cured tobacco varieties with different resistance to black shank disease were significantly reduced，and the control effects for highly susceptible variety Jingyehuang，moderately resistant variety K326 and highly resistant variety NC82 were 56.52%，31.00% and 27.00% respectively. Under the condition of no biochar application，the fungal diversity of rhizosphere soil of high‑resistance variety NC82 and high‑sensitivity variety Jingyehuang was relatively higher，while that of middle‑resistance variety K326 was relatively lower. After biochar application，the fungal diversity of middle‑resistance variety K326 increased significantly，while the fungal diversity of high‑sensitivity variety Jingyehuang and high‑resistance variety NC82 decreased in different degrees. LefSe analysis showed that the key microbial groups in rhizosphere soil of different resistant flue‑cured tobacco varieties were significantly different without biochar application，and the application of biochar changed the key microbial groups in rhizosphere soil，with obvious differences among varieties. PCoA analysis showed that under the condition of no biochar application，compared with the soil without tobacco planting，the sample points of three flue‑cured tobacco varieties were obviously separate，and the application of biochar changed the fungal community structure in rhizosphere soil of three flue‑cured tobacco varieties.To sum up，applying biochar can significantly reduce the occurrence of tobacco black shank disease.The rhizosphere soil fungal community structure of flue‑cured tobacco varieties with different resistance to black shank disease has different responses to biochar input. In production，variety factors should be considered when using biochar to improve soil and tobacco microecological environment. Reference | Related Articles | Metrics

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Research and Application of Chitosan Coatings in Fruits and Vegetables Storage and Preservation LIU Chenghong, ZHANG Shun, HUANG Wen, ZHANG Xiaoyan, YANG Hailong, FU Shiyu, ZHAN Lijuan Journal of Henan Agricultural Sciences    2025, 54 (7): 1-11.   DOI: 10.15933/j.cnki.1004-3268.2025.07.001 Abstract （1420）      PDF （1976KB）（2471）       Knowledge map    Save Fresh fruits and vegetables，due to their high water content and vigorous respiratory metabolism，are highly prone to quality decline and spoilage after harvest.As the world’s largest producer and consumer of fruits and vegetables，China has a persistently high post‑harvest loss rate of fruits and vegetables.The development of efficient and safe preservation technology has become an urgent need for the industry.Coating preservation technology is a new food preservation method，which can effectively maintain the quality of fresh fruits and vegetables and extend the shelf life of fruits and vegetables.Chitosan，as a natural biopolymer，is regarded as the most promising preservation coating material due to its wide source，degradability and biosafety，as well as excellent film forming property，antibacterial activity and biocompatibility.A systematic review is conducted on the basic characteristics of chitosan and the preparation methods of coatings.The research and application of chitosan coatings in the field of fruit and vegetable preservation are described in detail.The preservation effect of chitosan coatings on fruits and vegetables is discussed from the perspectives of appearance quality，nutrients，flavor substances and shelf life，and mechanism of preservation and the key factors affecting the preservation efficiency are summarized.Finally，the preservative chitosan coating technical bottlenecks and future development directions are pointed out，with the expectation of providing theoretical references for the in‑depth research and industrial application of this technology. Table and Figures | Reference | Related Articles | Metrics

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Effects of Phosphorus Reduction Combined with Humic Acid and Biochar Application on Phosphorus Supply in Red Soil and Phosphorus Utilization by Maize Liu Zhijun, Bao Dejue, Zhou Shiyong, Zhang Chenglong, Wu Dandan, Pu Zixian, Yin Lixiao, Zhou Feng Journal of Henan Agricultural Sciences    2026, 55 (9): 35-47.   DOI: 10.15933/j.cnki.1004-3268.2026.09.004 Abstract （1419）      PDF （15557KB）（19）       Knowledge map    Save To investigate the effects of humic acid and biochar addition on soil phosphorus supply and maize phosphorus uptake and utilization in red soil farmland under phosphorus reduction conditions，a randomized complete block design was adopted. Under 40% phosphorus reduction（P2，P2O5 90 kg/ha），four treatments were set up with different levels of humic acid（H1，10 000 kg/ha；H2，5 000 kg/ha）and biochar（B1，10 000 kg/ha；B2，5 000 kg/ha），using the local conventional phosphorus application rate（CK，P2O5 150 kg/ha） as the control.The effects of humic acid and biochar addition under phosphorus reduction on the physicochemical properties of red soil，maize phosphorus uptake，and yield were studied.The results showed that，compared with the CK treatment，the addition of humic acid and biochar could，to varying degrees，reduce soil bulk density in the 0—30 cm plough layer，increase soil total porosity and capillary water holding capacity，raise red soil pH and cation exchange capacity（CEC），improve the physicochemical properties of the plough layer soil，increase soil organic matter content，and significantly affect the activities of acid phosphatase（ACP）and alkaline phosphatase（ALP）.Among them，both P2H1 and P2B1 treatments increased soil total phosphorus and available phosphorus contents. Compared with CK，at the trumpet stage，total phosphorus content in P2H1 and P2B1 treatments increased significantly by 35.35% and 35.40%，respectively，and available phosphorus content increased by 13.92% and 35.69%，respectively；at the dough stage，total phosphorus content in P2H1 and P2B1 treatments increased by 6.38% and 48.94%，respectively，with the P2B1 treatment showing a significant difference compared with CK.Compared with CK，at the dough stage，root and stem biomass and root‑to‑shoot ratio of P2H1 and P2B1 treatments increased significantly by 24.61%，3.81%，24.65% and 16.65%，12.39%，9.75%，respectively；maize yield increased by 8.46% and 12.03%，respectively；and leaf phosphorus uptake increased significantly by 22.14% and 37.57%，respectively.Correlation analysis of maize yield and phosphorus uptake with biomass and soil factors revealed that alkaline phosphatase activity and organic matter content were extremely significantly positively correlated with total phosphorus and available phosphorus content；soil total porosity was significantly positively correlated with total phosphorus content and extremely significantly positively correlated with available phosphorus content；CEC and soil capillary water holding rate were significantly positively correlated with available phosphorus content；yield was significantly positively correlated with total phosphorus content and positively correlated with available phosphorus content； phosphorus uptake was positively correlated with total phosphorus content.Comprehensively，humic acid and biochar improve the phosphorus supply capacity of red soil by regulating soil physicochemical properties and phosphatase activities，ultimately promoting efficient phosphorus uptake and utilization by maize. Under 40% phosphorus reduction，the application of 10 000 kg/ha biochar can effectively improve the physicochemical properties of red soil，promote phosphorus supply and maize phosphorus uptake，and ensure maize growth，development and yield formation. Table and Figures | Reference | Related Articles | Metrics

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Identification of Lasioderma serricorne in Tobacco Leaf Raw Materials Based on Improved YOLOv3 Algorithm ZHANG Weizheng, CHEN Saiyueyang, WANG Yanling, TIE Jinxin, DING Jia, LI Meng, LI Canlin, SU Xiaoke, GAN Yong Journal of Henan Agricultural Sciences    2023, 52 (11): 157-166.   DOI: 10.15933/j.cnki.1004-3268.2023.11.017 Abstract （1416）      PDF （10647KB）（146）       Knowledge map    Save In order to solve the problem of low efficiency and inaccuracy in manual recognition of tobacco beetles（Lasioderma serricorne）in tobacco leaf raw materials，and achieve accurate and intelligent recognition of tobacco beetles，a tobacco beetle recognition model was proposed based on the improved YOLOv3 algorithm.Firstly，Random Mix data augmentation technology was used to expand the tobacco beetle dataset；By introducing the K⁃means++ algorithm to recluster anchor boxes，YOLOv3’s recognition ability for tobacco beetles was improved；SIoU Loss was used to improve the Loss function of YOLOv3 bounding box to improve the accuracy of model positioning and accelerate model convergence；Finally，a feature refinement module was added to filter conflict information and optimize the accuracy of the model in identifying small targets in the tobacco beetle dataset.The results showed that，the improved YOLOv3 network model achieved average detection accuracy，recall，F1 score，and mAP of 93.26%，88.25%，0.90，and 94.59% for tobacco beetles in the test set，respectively，increased by 12.21 percentage points，11.79 percentage points，0.12，and 12.40 percentage points compared to the existing YOLOv3 algorithm，providing support for the effective recognition of tobacco beetles. Reference | Related Articles | Metrics

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Advances in Biochar Production Process and Its Effects on Soil Properties Jin Long, Haider Sultan, Jiang Qiaohan, Zhang Yunbo Journal of Henan Agricultural Sciences    2026, 55 (9): 8-25.   DOI: 10.15933/j.cnki.1004-3268.2026.09.002 Abstract （1389）      PDF （3235KB）（35）       Knowledge map    Save Biochar exhibits multidimensional effects of soil conditioning，nutrient retention，and quality enhancement in soil improvement.This article systematically reviewed the regulatory laws of raw material types，pyrolysis temperature，heating rate，and residence time on the physicochemical properties of biochar and their impact mechanisms on soil properties.Existing studies had shown that raw materials determined the fundamental properties of biochar，with woody biochar having the highest yield and optimal pore structure，which was beneficial for water and fertilizer retention；livestock manure biochar was rich in nutrients and was a high‑quality slow‑release fertilizer carrier.The pyrolysis temperature was the core driving force for regulating the physicochemical characteristics of biochar.Low temperature pyrolysis was beneficial for retaining acidic functional groups，medium temperature pyrolysis could increase the specific surface area and cation exchange capacity of biochar，and high temperature pyrolysis could enhance the long‑term stability and alkaline neutralization effect of carbon.Meanwhile，slow heating mode and extended residence time could effectively promote the formation of micropores inside biochar.At the level of soil improvement，the compatibility between the preparation process and the physical and chemical properties of soil directly determined the effectiveness of biochar improvement.Among them，biochar prepared by slow heating at medium temperature had the most significant effect on reducing soil bulk density. Compared with manure based biochar，wood and shell biochar had a better effect on increasing the effective water content of sandy soil.Biochar regulated soil biological properties by providing microbial habitat and nutrient supply for soil microorganisms，and its impact on enzyme activity exhibited a feedback mechanism that varied depending on the enzyme. In the future，the focus should be on the construction of big data prediction models for process‑characteristics‑function，analysis of the long‑term evolution of soil‑crop systems and their micro ecological mechanisms，as well as the creation of customized biochar based new products for specific scenarios. Table and Figures | Reference | Related Articles | Metrics

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Effects of Addition Amounts，Treatment Durations and Pretreatment Durations of Different Chalcogens on Cadmium Absorption and Transport of Wheat Seedlings under Cadmium Stress ZHOU Jinghang, WANG Zenghao, ZHANG Lei, ZHANG Xia, LIU Jiajia, HU Tiezhu, ZHANG Dazhong Journal of Henan Agricultural Sciences    2026, 55 (6): 1-10.   DOI: 10.15933/j.cnki.1004-3268.2026.06.001 Abstract （1359）      PDF （2703KB）（102）       Knowledge map    Save To explore the effects of chalcogens（sulfur，selenium and tellurium） on cadmium（Cd）absorption and transport of wheat seedlings，the effects of different types［Na2SO4（sulfate，SVI），Na2SO3（sulfite，SIV），Na2SeO4（selenate，SeVI），Na2SeO3（selenite，SeIV），and Na2TeO3（thioselenite，TeIV）］，addition amounts（600，1 200，2 400 μmol/L for SVI and SIV，and 5，15，30 μmol/L for SeVI，SeIV，and TeIV），treatment durations（2，6，12，24，48，96，168 h）and pretreatment durations（0，24，72，168 h）of chalcogens on Cd absorption kinetics，content and translocation factor of wheat seedlings were studied.The results showed that compared with Cd treatment alone（control），SVI，SeVI and SeIV had a relatively smaller impact on the Cd absorption rate and the maximum absorption rate（Vmax）. However，SIV and TeIV had a greater impact on the Cd absorption rate and Vmax，among which the 1 200 μmol/L SIV and 15 μmol/L TeIV treatments had the higher Vmax，with 88.1% and 54.4% higher than the control，respectively.The 600 μmol/L SIV treatment had the highest Cd Michaelis constant（Km），which was 39.7% higher than the control；while Km of SeIV and TeIV treatments decreased. The addition of chalcogens could significantly reduce Cd contents of root and shoot.Among them，SeIV had the greatest impact on Cd content of root，Cd contents of roots of 5，15，and 30 μmol/L SeIV treatments decreased by 56.1%，61.2%，and 61.5% compared with the control，respectively. Compared with the control，the Cd translocation factor of 1 200 and 2 400 μmol/L SVI treatments significantly decreased by 2.4% and 14.0%，respectively.Chalcogens promoted Cd absorption of root when the treatment duration was ≤24 h，inhibited Cd absorption of root when the treatment duration was ≥48 h，and reduced Cd content of root by 34.9%（SVI）to 49.1%（SeIV）compared with the control at 168 hours. Chalcogens significantly decreased Cd content of shoot when the treatment duration was ≥96 h，the reducing effect of SeVI and TeIV with the treatment duration ≥48 h on the Cd content of shoot was more significant. Compared with the control，the 168 h treatment of SeVI and TeIV reduced Cd content of shoot by 48.4% and 50.7%，respectively.When the duration of chalcogen treatment was ≤96 h，the Cd translocation factor was significantly higher than or not significantly different from the control. When the duration of chalcogen treatment was 168 h，the Cd translocation factor of SeVI and TeIV treatments significantly decreased compared with the control.Chalcogen pretreatment could reduce Cd contents of root and shoot. With the pretreatment time extension，the Cd contents of root and shoot under SIV treatment，the Cd content of shoot and Cd translocation factor under SVI treatment，and Cd translocation factor under TeIV treatment all showed decrease trend.At 168 h of pretreatment，Cd translocation factors of SVI and TeIV decreased by of 33.9% and 7.1% compared with the control，respectively. In conclusion，the effects of chalcogens on the Cd absorption and transport are influenced by type，addition amount，treatment duration and pretreatment duration.The 1 200，2 400 μmol/L SVI and 15 μmol/L TeIV reduce Cd translocation factor through enhancing the Cd fixation in roots. Treatment with chalcogens for more than 48 h obviously decreases Cd content of root，and more than 96 h significantly decreases Cd content of shoot. Chalcogens pretreatment can reduce Cd contents of root and shoot，and 168 h of SVI pretreatment has the lowest Cd translocation factor，which is 33.9% lower than the control. Table and Figures | Reference | Related Articles | Metrics

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Effect of Nitrogen and Zinc Application on Accumulation and Distribution of Mineral Elements in Plant of Maize Varieties with Different Zinc Efficiency ZHANG Panpan, QIAO Jiangfang, LI Chuan, ZHANG Meiwei, MU Weilin, GUO Hanxiao, ZHOU Yanli, CHENG Zeqiang Journal of Henan Agricultural Sciences    2024, 53 (6): 1-10.   DOI: 10.15933/j.cnki.1004-3268.2024.06.001 Abstract （1356）      PDF （2797KB）（334）       Knowledge map    Save With Zhengdan 958(ZD958)with high zinc(Zn)efficiency and Longping 638(LP638)with Zn insensitivity as materials,the field plot experiment was conducted with three nitrogen(N)rates[90 kg/ha(LN),180 kg/ha(MN)and 225 kg/ha(HN)]and two foliar Zn treatments[no Zn(F0),spraying Zn of 1∶1 at jointing stage and flare opening stage(F1)]to analyze the effects of N and Zn application on content,accumulation and distribution of mineral elements in plant and grain yield of maize,so as to provide reference for N and Zn application in the maize production.The results showed that the highest copper(Cu)and Zn content in grain was found under MN treatment,with 1.82 mg/kg and 16.23 mg/kg,respectively.F1 treatment could increase the contents of N and Zn and Zn availability.The accumulation of N,phosphorus(P)and potassium(K)in plant was the highest in LP638 under MNF1 treatment;The highest accumulation of Cu and Zn was found in ZD958 under LNF1 treatment,while the highest accumulation of manganese(Mn)and iron(Fe)under HNF1 treatment.The highest proportion of N,P and Cu in grain was obtained in LP638 under HNF1 treatment;The higher proportion of Zn was found in ZD958 under HNF0 and LNF0 treatments.HN treatment had the highest grain yield with an average of 10.31 t/ha,followed by MN treatment,and LN treatment was the lowest.There was significantly positive correlation between P content and Mn content in grain of two varieties;K content was significantly positively correlated with Fe content and Zn content,but significantly negatively correlated with Cu content in LP638.To sum up,180 kg/ha N and spraying Zn of 1∶1 at jointing stage and flare opening stage can increase Zn availability and accumulation of mineral elements in maize grain，promote the synchronous improvement of yield and mineral nutrition,and can be promoted in maize field production. Reference | Related Articles | Metrics