The Complete Gene Sequencing of Bacilus subtilis Isolated from Rehmannia glutinosa and Study of Genes Related to Growth‑promoting Effects

doi:10.15933/j.cnki.1004-3268.2024.12.008

Abstract

Abstract: To investigate the genes associated with the growth‑promoting effects of Bacillus subtilis in Rehmannia glutinosa，whole genome sequencing was performed in this study and the genes associated with the growth‑promoting effects were discovered through functional annotation.The results showed that the genome size of Bacillus subtilis，an endophytic growth‑promoting bacterium of Rehmannia glutinosa，was 4 310 699 bp，with a GC content of 43.44%，and a total of 4 521 predicted coding genes.A total of 3 013，3 354，4 395，206，52，and 212 functional genes were annotated in the clusters of orthologous groups（COG），gene ontology（GO），kyoto encyclopedia of genes and genomes （KEGG），carbohydrate‑active enzymes（CAZyme），comprehensive antibiotic resistance database（CARD），and virulence factor database（VFDB），respectively.The annotation results indicated that there were 46 genes related to growth‑promoting function，with the majority of these genes involved in phosphorus solubilization，nitrogen fixation，and iron carrier processes.In the meantime，anti‑SMASH predicted that a total of 409 genes were enriched into 12 secondary metabolite synthesis gene clusters，with the majority encoding eight repressors，including fengycin，1‑carbapen‑2‑em‑3‑carboxylic acid，bacillaene，bacilysin，subtilosin A，surfactin，pulcherriminic acid，and bacillibactin.Among these，pulcherriminic acid and bacillibactin had been observed to facilitate the chelation of ferric ions in the surrounding environment.

Key words: Bacilus subtilis, Rehmannia glutinosa, Whole genome sequencing, Functional annotation, Endophytic bacteria, Growth?promoting gene

摘要： 为了探究地黄枯草芽孢杆菌（Bacilus subtilis）对地黄促生作用的相关基因，对其进行全基因组测序，并通过功能注释挖掘促生作用相关基因。结果表明，地黄内生促生菌枯草芽孢杆菌基因组大小为4 310 699 bp，GC含量为43.44%，共预测编码基因4 521个。GO、COG、KEGG、CAZy、CARD和VFDB数据库注释的基因数分别为3 013、3 354、4 395、206、52、212。与促生作用有关基因有46个，这些基因主要集中在溶磷、固氮、铁载体等方面。同时，antiSMASH预测到共409个基因富集到12个次级代谢产物合成基因簇，主要编码合成丰原素、3-羧酸碳青霉烯、类双硫醇、杆菌溶素、羊毛硫类细菌素、表面活性肽、美好菌素酸和儿茶酚型嗜铁素等8种抑菌物质，其中美好菌素酸和儿茶酚型嗜铁素还具有螯合周围环境铁离子的作用。

关键词: 枯草芽孢杆菌, 地黄, 全基因组测序, 功能注释, 内生菌, 促生基因

CLC Number:

S567

LA Guixiao, WANG Linlin, GUO Junqi, ZHAO Yulong, GUO Hongxia, JIA Hui, YANG Tiegang. The Complete Gene Sequencing of Bacilus subtilis Isolated from Rehmannia glutinosa and Study of Genes Related to Growth‑promoting Effects[J]. Journal of Henan Agricultural Sciences, 2024, 53(12): 75-85.

腊贵晓, 王林林, 郭军旗, 赵玉龙, 郭红霞, 贾慧, 杨铁钢. 地黄枯草芽孢杆菌全基因组测序及其促生作用相关基因研究[J]. 河南农业科学, 2024, 53(12): 75-85.

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