Identification and Indoor Fungicide Screening of Pathogen Causing Leaf Spot on Rehmannia glutinosa

doi:10.15933/j.cnki.1004-3268.2024.04.010

Abstract

Abstract: In order to identify the pathogen species and its biological characteristics of Rehmannia glutinosa leaf spot in Jizhou area of Tianjin City,and to screen effective pesticides for controlling the pathogen of R.glutinosa leaf spot，the methods of diseased leaves collection,strain isolation，microscopic observation,in vitro inoculation,ITS,EF1‑α,Tub multiple genes sequence alignment and evolutionary tree analysis were used to islolate,purify and identify the pathogen of R.glutinosa leaf spot by morphology,pathogenicity and molecular biology.The biological characteristics of the pathogen in three media and four carbon and nitrogen sources were studied.At the same time,the virulence of four fungicides was determined.The results showed that the pathogen A1 isolated from the leaf spot of R.glutinosa was Fusarium equiseti.The optimum medium,nitrogen source and carbon source were Czapek medium,glycine and sucrose,respectively.The results of laboratory efficacy test showed that 45% prochloraz aqueous emulsion had the best inhibitory effect on mycelial growth of pathogen,the concentration for 50% of maximal effect(EC₅₀)was 63.69 mg/L,and the inhibition rate of mycelial growth was up to 83.64%.75% chlorothalonil wettable powder had the worst inhibitory effect,with EC₅₀ of 355.06 mg/L,and the maximum inhibitory rate of mycelial growth was 64.63%.In conclusion,F.equiseti was the pathogen of R.glutinosa leaf spot in Jizhou district of Tianjin,and 45% prochloraz aqueous emulsion has the best controlling effect on the pathogen of R.glutinosa leaf spot.

Key words: Rehmannia glutinosa, Leaf spot, Fusarium equiseti, Biological characteristics, Screening of fungicides

摘要： 为明确天津市蓟州区地黄叶斑病病原种类及生物学特性，筛选防治地黄叶斑病病原菌的有效农药，通过采集病叶、菌株分离、显微观察、离体回接以及ITS、EF1-α、Tub多基因序列比对和进化树分析的方法，对地黄叶斑病致病菌进行分离纯化、形态学鉴定、致病性鉴定以及分子生物学鉴定，并对分离菌在3种培养基、4种碳氮源条件下的生物学特性进行研究，同时选取4种杀菌剂对其进行毒力测定。结果表明，从天津市蓟州区地黄叶斑病发病叶片部位分离出的病原菌A1 为木贼镰孢菌（Fusarium equiseti）。生物学特性研究表明，该菌最适培养基、氮源、碳源分别为Czapek培养基、甘氨酸、蔗糖。室内药效检测结果表明，45%咪鲜胺水乳剂对该病原菌的抑制效果最好，半效应质量浓度（EC₅₀）为63.69 mg/L，对病原菌菌丝生长的抑制率最高可达83.64%；75%百菌清可湿性粉剂的抑制效果最差，EC₅₀为 355.06 mg/L，对病原菌菌丝生长的抑制率最高为64.63%。综上，天津市蓟州区地黄叶斑病病原菌为木贼镰孢菌（F.equiseti），45%咪鲜胺水乳剂对地黄叶斑病病原菌的防治效果最好。

关键词: 地黄, 叶斑病, 木贼镰孢菌, 生物学特性, 药剂筛选

CLC Number:

S435.672

LI Haicheng, WANG Fanzhen, JIANG Yongcheng, SONG Xin, CHEN Zhaorong. Identification and Indoor Fungicide Screening of Pathogen Causing Leaf Spot on Rehmannia glutinosa[J]. Journal of Henan Agricultural Sciences, 2024, 53(4): 92-101.

李海骋, 王繁珍, 姜永成, 宋欣, 陈招荣. 地黄叶斑病病原鉴定及室内药效检测[J]. 河南农业科学, 2024, 53(4): 92-101.

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