Growth Response and Tolerance Evaluation of 32 Spinach Varieties to Cadmium Stress

doi:10.15933/j.cnki.1004-3268.2024.09.011

Journal of Henan Agricultural Sciences ›› 2024, Vol. 53 ›› Issue (9): 108-116.DOI: 10.15933/j.cnki.1004-3268.2024.09.011

• Horticulture • Previous Articles Next Articles

Growth Response and Tolerance Evaluation of 32 Spinach Varieties to Cadmium Stress

MIAO Mingjun^1,2,3，LI Jiajia¹，LEI Xiaokui^1,3，LIN Yang^1,3，LIAO Jichao¹，ZHONG Jian^1,3，LI Ju^1,2,3，LI Yuejian^1,2,3，CHANG Wei^2,3,4

（1.Horticulture Research Institute，Sichuan Academy of Agricultural Sciences/Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province，Chengdu 610066，China；2.Sichuan Vegetable Engineering Technology Research Center，Chengdu 610066，China；3.Horticultural Crops Biology and Germplasm Enhancement Key Laboratory in Southwest Regions of Ministry of Agriculture and Rural Affairs，Chengdu 610066，China；4.Sichuan Institute of Edible Fungi，Chengdu 610066，China）

Received:2024-04-18 Published:2024-09-15 Online:2024-11-04

32 个菠菜品种对镉胁迫的生长响应及耐镉性评价

苗明军^1,2,3，李佳佳¹，雷晓葵^1,3，林杨^1,3，廖继超¹，钟建^1,3，李菊^1,2,3，李跃建^1,2,3，常伟^2,3,4

（1.四川省农业科学院园艺研究所/蔬菜种质创新与品种改良四川省重点实验室，四川成都 610066；2.四川省蔬菜工程技术研究中心，四川成都 610066；3.农业农村部西南地区园艺作物生物学与种质创制重点实验室，四川成都 610066；4.四川省食用菌研究所，四川成都 610066）

通讯作者: 常伟（1974-），男，湖北钟祥人，研究员，硕士，主要从事蔬菜育种与栽培研究。E-mail：471243498@qq.com
作者简介:苗明军（1983-），男，河南漯河人，副研究员，博士，主要从事蔬菜育种与栽培生理研究。E-mail：1224566145@qq.com
基金资助:
国家现代农业产业技术体系四川大宗蔬菜创新团队资助项目（SCCXTD-2024-5）；阿坝州特色产业提质增效关键技术示范与推广项目（2023ZSSFXD13）；彭州市蔬菜绿色高效生产关键技术研究与示范项目（2023PZSC0306）；成都市蔬菜产业化育苗及绿色高效生产关键技术研究、应用与示范项目（2022-YF09-00051-SN）

Abstract

Abstract: Cadmium(Cd)is a highly toxic heavy metal element.To identify Cd‑tolerant spinach varieties and screen related identification indexes,and provide a theoretical basis for the selection and breeding of Cd‑tolerant varieties,in the present study,we conducted a hydroponic experiment using 32 spinach varieties in China.The spinach was exposed to Cd stress(Cd concentration of 50 μmol/L)for 20 days,while the other group of plants was treated as a control(Cd concentration of 0 μmol/L).After the plants reached maturity,we measured the agronomic traits and Cd content.The integrated Cd tolerance of each spinach variety was evaluated by principal component analysis,affiliation function analysis,and correlation analysis.The results showed that plant height,root length,underground fresh weight,aboveground fresh weight,aboveground Cd content,underground Cd content and translocation coefficient of the participating varieties under Cd stress conditions were significantly different from the control.Under Cd stress,the growth and biomass accumulation of spinach were inhibited,and the indicators of each trait decreased significantly.The order of average reduction of each agronomic index was aboveground fresh weight>plant height>underground fresh weight>root length.The principal component analysis of Cd stress tolerance index of the seven indexes was performed,and three principal components were selected，with a cumulative variance contribution of 81.552%;The composite evaluation value(D value)showed highly significant positive correlations(P<0.01)with plant height,underground fresh weight,aboveground fresh weight,aboveground Cd content,and translocation coefficient,which can be used as the preferred indexes for the evaluation of Cd tolerance in spinach.Based on the principal component analysis,we classified the 32 spinach varieties into three categories:Cd‑tolerant,moderate,and Cd‑sensitive varieties.Among the varieties tested,Jin’ge and Micro orchid exhibited strong Cd tolerance,while Desai 176 was found to be Cd‑sensitive.

Key words: Spinach, Cd tolerance, Cd uptake, Affiliation function method, Principal component analysis, Comprehensive evaluation

摘要： 镉（Cadmium，Cd）是一种剧毒重金属元素，鉴定耐Cd菠菜品种并筛选相关鉴定指标，为耐Cd菠菜品种的选育提供理论依据。选用国内32个菠菜品种，采用Hoagland水培溶液加入CdCl2·2.5H2O模拟大田试验，在Cd胁迫（Cd浓度50 μmol/L）和对照（Cd浓度0 μmol/L）处理20 d，待菠菜成熟期，测定其农艺性状和Cd含量。通过主成分分析、隶属函数分析、相关性分析评价各菠菜品种的综合耐Cd能力。结果表明，Cd胁迫条件下参试品种的株高、根长、地下部鲜质量、地上部鲜质量、地上部Cd含量、地下部Cd含量和转运系数与对照相比均存在显著差异；Cd胁迫下，菠菜的生长、生物量积累均受到抑制，各性状指标均明显下降，各农艺指标平均降幅排序依次为地上部鲜质量>株高>地下部鲜质量>根长。对7个指标的耐Cd系数进行主成分分析，选择了3个主成分，累计方差贡献率达81.552%；综合评价值（D值）与株高、地下部鲜质量、地上部鲜质量、地上部Cd含量、转运系数均呈极显著正相关关系（P<0.01），这些指标可以作为菠菜耐Cd能力评价的首选指标。根据主成分分析，将32份菠菜品种划分为耐Cd性强、中等和Cd敏感型3种类型，金格和微兰为耐Cd性强品种，德赛176为Cd敏感品种。

关键词: 菠菜, 耐Cd性, Cd吸收, 隶属函数法, 主成分分析, 综合评价

CLC Number:

S636.1

MIAO Mingjun, LI Jiajia, LEI Xiaokui, LIN Yang, LIAO Jichao, ZHONG Jian, LI Ju, LI Yuejian, CHANG Wei. Growth Response and Tolerance Evaluation of 32 Spinach Varieties to Cadmium Stress[J]. Journal of Henan Agricultural Sciences, 2024, 53(9): 108-116.

苗明军, 李佳佳, 雷晓葵, 林杨, 廖继超, 钟建, 李菊, 李跃建, 常伟. 32 个菠菜品种对镉胁迫的生长响应及耐镉性评价[J]. 河南农业科学, 2024, 53(9): 108-116.

References

［1］MISHRA S，BHARAGAVA R N，MORE N，et al.Heavy metal contamination：An alarming threat to environment and human health［M］//SOBTI R，ARORA N，KOTHARI R.Environmental biotechnology：For sustainable future. Singapore：Springer，2019：103‑125.

［2］SHI J D，ZHAO D，REN F T，et al.Spatiotemporal variation of soil heavy metals in China：The pollution

status and risk assessment［J］.Science of the Total Environment，2023，871：161768.

［3］REN S Y，SONG C O，YE S J，et al.The spatiotemporal variation in heavy metals in China´s farmland soil over the past 20 years：A meta‑analysis［J］.The science of the Total Environment，2022，806（Pt 2）：150322.

［4］彭秋，李桃，徐卫红，等.不同品种辣椒镉亚细胞分布和化学形态特征差异［J］.环境科学，2019，40（7）：3347‑3354.

Peng Q，Li T，Xu W H，et al.Differences in the cadmium‑enrichment capacity and subcellular distribution and chemical form of cadmium in different varieties of pepper［J］.Environmental Science，2019，40（7）：3347‑3354.

［5］罗洋，刘方，任军.重金属污染土壤的植物修复效果评价方法研究进展［J］.应用化工，2020，49（3）：755‑760.

LUO Y，LIU F，REN J.Research progress on evaluation methods of phytoremediation effectiveness of soil contaminated with heavy metal［J］.Applied Chemical Industry，2020，49（3）：755‑760.

［6］范利荣，晁赢，阎祥慧，等.镉胁迫对不同谷子品种种子萌发特性的影响［J］.草业科学，2023，40（9）：2330‑2339.

FAN L R，CHAO Y，YAN X H，et al.Effects of cadmium stress on seed germination characteristics of 58 millet accessions［J］.Pratacultural Science，2023，40（9）：2330‑2339.

［7］王洁，朱学栋，孙冲，等.生姜品种对镉胁迫的生理响应及耐镉性评价［J］.农业环境科学学报，2023，42（11）：2410‑2421.

WANG J，ZHU X D，SUN C，et al.Physiological response and cadmium tolerance evaluation of ginger cultivars to cadmium stress［J］.Journal of Agro‑Environment Science，2023，42（11）：2410‑2421.

［8］杜含梅，谭露，陈勃，等.苦荞苗期镉耐受性综合评价［J］.作物杂志，2024，40（2）：40‑53.

DU H M，TAN L，CHEN B，et al.Comprehensive evaluation of cadmium tolerance of tartary buckwheat at seedling stage［J］.Crops，2024，40（2）：40‑53.

［9］BERGQUIST S A M，GERTSSON U E，KNUTHSEN P，et al.Flavonoids in baby spinach（Spinacia oleracea L.）：Changes during plant growth and storage［J］.Journal of Agricultural and Food Chemistry，2005，53（24）：9459‑9464.

［10］BELIĆ M，ZDRAVKOVIĆ‑KORAĆ S，UZELAC B，et al.Variability in somatic embryo‑forming capacity of spinach［J］.Scientific Reports，2020，10（1）：19290.

［11］BASHIR S，ALI U，SHAABAN M，et al.Role of sepiolite for cadmium（Cd）polluted soil restoration and spinach growth in wastewater irrigated agricultural soil［J］.Journal of Environmental Management，2020，258：110020.

［12］李春萍，覃德华，文晓阳，等.镉胁迫对不同品种烟草生长发育和镉吸收规律的影响［J］.广东农业科学，2023，50（4）：22‑32.

LI C P，QIN D H，WEN X Y，et al.Effect of cadmium stress on the growth and cadmium absorption rules of

different tobacco varieties［J］.Guangdong Agricultural Sciences，2023，50（4）：22‑32.

［13］陈炬廷，毛云，杨佳豪，等.KOH改性烟秆生物炭对铅、镉复合污染土壤中烤烟铅、镉吸收的影响［J］.南方农业学报，2024，55（2）：460‑467.

CHEN J T，MAO Y，YANG J H，et al.Effects of KOH modified tobacco stalk biochar on lead and cadmium absorption in flue‑cured tobacco in lead and cadmium composite polluted soil［J］.Journal of Southern Agriculture，2024，55（2）：460‑467.

［14］贺远，伍宗毅，陈国瑀，等.不同pH和镉浓度对雪茄烟种子萌发和幼苗生长生理的影响［J］.南方农业学报，2023，54（8）：2236‑2244.

HE Y，WU Z Y，CHEN G Y，et al.Effects of different pH and cadmium concentrations on physiology of seed germination and seedling growth of cigar［J］.Journal of Southern Agriculture，2023，54（8）：2236‑2244.

［15］张志芳，豁泽春.镉、砷胁迫下接种丛枝菌根真菌对烤烟镉、砷累积及生理特性的影响［J］.河南农业科学，2022，51（2）：47‑56.
ZHANG Z F，HUO Z C.Effects of arbuscular mycorrhizal fungi inoculation on cadmium，arsenic accumulation and physiological characteristics of flue‑cured tobacco under cadmium and arsenic stress［J］.Journal of Henan Agricultural Sciences，2022，51 （2）：47‑56.

［16］何雪，陆引罡，张洁，等.镉胁迫下不同土壤类型对马铃薯生长及镉富集转运特性的影响［J］.地球与环境，2023，51（1）：87‑101.
HE X，LU Y G，ZHANG J，et al.Effects of soil types on potato growth and cadmium accumulation and transport characteristics under cadmium stress［J］.Earth and Environment，2023，51（1）：87‑101.

［17］柴冠群，周礼兴，王丽，等.镉砷污染耕地玉米重金属安全品种筛选［J］.河南农业科学，2022，51（10）：74‑85.
CHAI G Q，ZHOU L X，WANG L，et al.Screening of safe maize varieties of heavy metals in cadmium arsenic contaminated farmland［J］.Journal of Henan Agricultural Sciences，2022，51（10）：74‑85.

［18］王丽，蔡景行，罗沐欣键，等.镉胁迫下钝化剂对菠菜生理特征及镉累积的影响［J］.农业环境科学学报，2023，42（5）：1004‑1010.
WANG L，CAI J H，LUO MXJ，et al.Effects of passivating agents on physiological characteristics and cadmium accumulation in Spinacia oleracea L.under cadmium stress［J］. Journal of Agro‑Environment Science，2023，42（5）：1004‑1010.

［19］陈花，王建军，杨斌.镉胁迫对菠菜和油菜早期生长的影响［J］.山西农业科学，2016，44（9）：1308‑1311.
CHEN H，WANG J J，YANG B.Effects of Cd stress on the early growth of spinach and rape［J］.Journal of Shanxi Agricultural Sciences，2016，44（9）：1308‑1311.

［20］黄婷，周园园，龚奕杰，等.昆山地区樱桃番茄新品种筛选试验及综合评价［J］.中国种业，2023（11）：77‑84.
HUANG T，ZHOU Y Y，GONG Y J，et al.Screening test and comprehensive assessment on new cherry tomatoes in Kunshan area［J］. China Seed Industry，2023（11）：77‑84.

［21］陈天笑，白小刚，何茜，等.缺氮胁迫下不同黑木相思基因型综合评价［J］.中南林业科技大学学报，2023，43（10）：47‑59.
CHEN T X，BAI X G，HE Q，et al.Comprehensive evaluation in different genotypes of Acacia melanoxylon under nitrogen deficiency stress［J］.Journal of Central South University of Forestry &Technology，2023，43（10）：47‑59.

［22］LI J J，LIU X Y，YAO Q，et al.Tolerance and adaptation characteristics of sugar beet（Beta vulgaris L.） to low nitrogen supply［J］.Plant Signaling &Behavior，2023，18（1）：2159155.

［23］白金顺，王雪翠，王艳秋.箭筈豌豆种质资源萌发期抗旱指标筛选及抗旱性评价［J］.植物营养与肥料学报，2020，26（12）：2253‑2263.
BAI J S，WANG X C，WANG Y Q.Screening of drought‑resistance index and drought‑resistance evaluation of common vetch（Vicia sativa L.）germplasms at germination stage［J］.Journal of Plant Nutrition and Fertilizers，2020，26（12）：2253‑2263.

［24］ALI E，HUSSAIN N，SHAMSI I H，et al.Role of jasmonic acid in improving tolerance of rapeseed（Brassica napus L.）to Cd toxicity［J］.Journal of Zhejiang University Science B，2018，19（2）：130‑146.

［25］REPKINA N，MURZINA S A，VORONIN V P，et al.Does methyl jasmonate effectively protect plants under heavy metal contamination？ fatty acid content in wheat leaves exposed to cadmium with or without exogenous methyl jasmonate application［J］.Biomolecules，2023，13（4）：582.

［26］LI J J，LI W S，XU L Q，et al.Acclimation of sugarbeet in morphological，physiological and BvAMT1.2 expression under low and high nitrogen supply［J］.PLoS One，2022，17（11）：e0278327.

［27］邹金城，龚玉莲，陈丽红，等.根系分泌物对蕹菜Cd吸收积累的影响［J］.广东农业科学，2019，46（5）：60‑66.
ZOU J C，GONG Y L，CHEN L H，et al.Effects of root exudates on Cd absorption and accumulation in Ipomoea aquatica Forsk［J］.Guangdong Agricultural Sciences，2019，46（5）：60‑66.

［28］GONG Y L，YU J G，YANG Z Y，et al.Cadmium and lead accumulations by typical cultivars of water spinach under different soil conditions［J］.Fresenius Environmental Bulletin，2010，19：190‑197.

［29］KHAN K Y，ALI B，STOFFELLA P J，et al.Study amino acid contents，plant growth variables and cell ultrastructural changes induced by cadmium stress between two contrasting cadmium accumulating cultivars of Brassica rapa ssp.chinensis L.（pak choi）［J］. Ecotoxicology and Environmental Safety，2020，200：110748.

［30］ZHAO H Y，GUAN J L，LIANG Q，et al.Effects of cadmium stress on growth and physiological characteristics of sassafras seedlings［J］.Scientific Reports，2021，11（1）：9913.

［31］卢海，李增强，唐美琼，等.红麻DNA甲基化响应镉胁迫及甲基化差异基因的表达分析［J］.作物学报，2021，47（12）：2324‑2334.
LU H，LI Z Q，TANG M Q，et al.DNA methylation in response to cadmium stress and expression of different methylated genes in kenaf［J］.Acta Agronomica Sinica，2021，47（12）：2324‑2334.

［32］吴月莹，邓思情，刘松芹，等.镉胁迫对不同品种玉米幼苗生长及生理特性的影响［J］.湖南农业大学学报（自然科学版），2023，49（5）：509‑515.
WU Y Y，DENG S Q，LIU S Q，et al.Effects of cadmium stress on the growth and physiological characteristics of maize seedlings from different varieties［J］. Journal of Hunan Agricultural University（Natural Sciences），2023，49（5）：509‑515.

［33］汪京超，李楠楠，谢德体，等.砷在植物体内的吸收和代谢机制研究进展［J］.植物学报，2015，50（4）：516‑526.
WANG J C，LI N N，XIE D T，et al.Mechanisms of arsenic uptake and metabolism in plants［J］.Chinese Bulletin of Botany，2015，50（4）：516‑526.

[1]	ZHANG Bo, WANG Xinhui, KONG Bo, WANG Zhiguo, LI Ruili, LIANG Miao, ZHANG Junsong. Analysis of Separation Products from Alcohol‑Soluble Extract of Burley Tobacco by Different Apertures of Membrane and Cigarette#br# Flavoring Evaluation [J]. Journal of Henan Agricultural Sciences, 2024, 53(8): 165-172.
[2]	ZHANG Huifang, YAN Zhaoling, LIU Haijiao, DUAN Junzhi, CHEN Haiyan, GUO Yan, WANG Nan, YANG Cuiping, QI Hongzhi. Analysis and Evaluation of Comprehensive Traits of Wheat Varieties in Henan Province Consortium Experiment [J]. Journal of Henan Agricultural Sciences, 2024, 53(7): 35-43.
[3]	LIU Zhiyong, LUO Fufang, LU Wanhua, ZHU Bo, LI Hongxun, LIU Zhangyong, CHEN Jie, YI Lixia. Soil Quality Assessment of Tobacco Field Based on Minimum Data Set in Southwest Guizhou Autonomous Prefecture [J]. Journal of Henan Agricultural Sciences, 2024, 53(7): 100-108.
[4]	WANG Pengwei, YAN Xiaowen, LIU Zhenyu, SUN Li, LIU Zhenwei, LI Xinzheng. Comparison of Post‐harvest Quality Changes and Storage Toleranceof Baimi Series Pumpkin Varieties（Lines） [J]. Journal of Henan Agricultural Sciences, 2024, 53(3): 158-168.
[5]	QIAO Yuxuan, SHEN Xiaoxiao, JIAO Xuehui, ZHOU Xiaojuan, YUE Changping, SHI Xibing. Genetic Diversity Analysis of Ornamental Peach Germplasm Based on Phenotypic Traits [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 108-117.
[6]	WANG Xiaojie, LI Meixia, TAO Lei, ZHANG Bo, HE Xinru, MI Jia, DAI Guoli, XU Wendi. Study on the Genetic Diversity of Phenotypic and Quality Traits of 48 Germplasm Resources of Lycium ruthenicum Murr. [J]. Journal of Henan Agricultural Sciences, 2023, 52(9): 78-90.
[7]	DOU Dandan, SUN Jianjun, WANG Dexin, GUO Yuxi, GUO Xinhai, DING Chaoming. Effect of Sowing Date on Yield and Quality of Early and Middle Rice [J]. Journal of Henan Agricultural Sciences, 2023, 52(7): 12-23.
[8]	CHEN Han, HU Huiyun, CHENG Jianfeng, HUANG Yingjin, MAO Xiaorong, XU Xiaoming, ZENG Yanhua. Differences in Natural Enrichment Abilities of Elements in Root Tubers of Different Genotypes of Sweet Potato（Ipomoea batatas Lam.） [J]. Journal of Henan Agricultural Sciences, 2023, 52(7): 24-39.
[9]	WEI Yuan, LÜ Mengyang, MA Yate, LIU Jing, WANG Xuan, WANG Dongsheng. Comprehensive Evaluation of the Nutritional Quality of Chestnut Based on Three Methods [J]. Journal of Henan Agricultural Sciences, 2023, 52(6): 111-119.
[10]	ZHAO Haijun, SHI Jiaqing, WANG Bin, GUO Yiyang, HU Xiaoli, HAN Zanping. Comprehensive Evaluation of Grain and Its Quality Traits of 150 Maize Inbred Lines [J]. Journal of Henan Agricultural Sciences, 2023, 52(5): 33-39.
[11]	WU Guojiang, ZHOU Wei, YU Zhonghao, LI Yan, LÜ Jingbo, WANG Zhenguo, ZHOU Yaxing. Comprehensive Evaluation of 176 Waxy Sorghum Germplasm Resources Based on Principal Component，Grey Correlation and DTOPSIS Analyses [J]. Journal of Henan Agricultural Sciences, 2023, 52(5): 40-51.
[12]	CHEN Zedong, SHI Huanting, DONG Dianqi, WANG Yaxin, WANG Jinfeng, YU Bo, WANG Pengfei, KANG Guozhang. Comprehensive Evaluation of Low Phosphorus Tolerance of Different Wheat Varieties（Lines）and Screening of Representative Evaluation Indexes [J]. Journal of Henan Agricultural Sciences, 2023, 52(4): 21-30.
[13]	YU Gao, WANG Hai, MO Zhongmei, CHEN Fen. Rhizome Quality Characteristics of Polygonatum rhizoma in Different Habitats and Driving Factors in Rhizosphere Soil Environment [J]. Journal of Henan Agricultural Sciences, 2023, 52(12): 57-68.
[14]	MU Chunyu, ZHANG Rui, TANG Qingping, BU Zhu, CHANG Lingling, FU Shengyong, XIA Aiping, YANG Mingjun. Principal Component and Path Analysis of Body Size and Slaughter Performance of Different Days of Shiqi Pigeon [J]. Journal of Henan Agricultural Sciences, 2022, 51(9): 133-140.
[15]	ZHANG Fan, LI Yuan, CHEN Mengru, XING Yingying, WANG Xiukang. Comparison and Comprehensive Evaluation of Quality of 20 Potato Varieties [J]. Journal of Henan Agricultural Sciences, 2022, 51(8): 28-36.

Growth Response and Tolerance Evaluation of 32 Spinach Varieties to Cadmium Stress

32 个菠菜品种对镉胁迫的生长响应及耐镉性评价

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics