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    Journal of Henan Agricultural Sciences    2013, 42 (4): 179-183.  
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    Journal of Henan Agricultural Sciences    2015, 44 (2): 91-93,100.  
    Abstract23)      PDF (31024KB)(1177)       Save
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    Journal of Henan Agricultural Sciences    2011, 40 (2): 16-18.  
    Abstract228)      PDF (143KB)(855)       Save
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    Journal of Henan Agricultural Sciences    2019, 48 (8): 1-6.  
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    Analysis of Combining Ability and Heredity for Plant Yield Traits in Pepper
    HAN Ya’nan, CHANG Xiaoke, CHENG Zhifang, ZHANG Tao, YAO Qiuju
    Journal of Henan Agricultural Sciences    2021, 50 (7): 136-144.   DOI: 10.15933/j.cnki.1004-3268.2021.07.016
    Abstract288)      PDF (6392KB)(750)       Save
    In order to evaluate the combining ability and heredity for plant architecture and yield components,and improve the efficiency of selecting elite combinations of pepper(Capsicum annuum L.),five parental lines(C1—C5)with different characters were used to develop 20 hybrid crosses by Griffing complete diallel crossing design,and nine agronomic traits of 25 genetypes were determined to analyze heterosis,combining ability and heritability.The results showed that the heterosis indexes of yield per plant,fruit number per plant,plant width and fruit length were higher,with the averages of up to 145.98%,131.35% 122.51% and 121.20%,respectively. The coefficients of variation of yield per plant and fruit number per plant were 31.80% and 17.78%,respectively. Analysis of combination ability showed that C2 and C4 with high general combination ability(GCA)were the optimal lines in single fruit weight and single plant yield,and the crosses C4×C5,C1×C2,C2×C3 and C4×C5,C3×C2 were determined as the excellent combinations in weight and single plant yield respeitively based on special combination ability(SCA)and reciprocal(REC)effects. The high values of broad⁃sense heritability and narrow⁃sense heritability for yield per plant and fruit width indicated that these traits were mainly controlled by additive effects,and the environmental variances of stem diameter and flesh thickness were higher than their genetic variances. The coefficients between major traits and yield per plant suggested that the yield per plant was positively correlated with single fruit weight,fruit length and fruit number per plant. In conclusion,the parental lines C2,C4 and the crosses C3×C2,C4×C5 are suitable for cultivating highly⁃yielding varieties. Pepper breeding should be focused on single fruit weight,fruit length and fruit number per plant.
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    Journal of Henan Agricultural Sciences    2012, 41 (12): 20-23.  
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    Journal of Henan Agricultural Sciences    2012, 41 (2): 9-11,53.  
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    Journal of Henan Agricultural Sciences    2005, 34 (1): 9-12.   DOI: 10.3969/j.issn.1004-3268.2005.01.002
    Abstract57)      PDF (141KB)(680)       Save
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    Journal of Henan Agricultural Sciences    2010, 39 (4): 115-118.   DOI: 10.3969/j.issn.1004-3268.2010.04.032
    Abstract145)      PDF (211KB)(676)       Save
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    Expression and Purification of CRISPR/Cas13a and Establishment of Its Collateral RNase Activity Assay
    WANG Xun, ZHANG Yuhang, SUN Yaning, LI Qingmei, LI Ge, WANG Li, GUO Junqing, DENG Ruiguang, ZHANG Gaiping
    Journal of Henan Agricultural Sciences    2021, 50 (4): 147-153.   DOI: 10.15933/j.cnki.1004-3268.2021.04.019
    Abstract361)      PDF (4597KB)(645)       Save
    To estabish LwaCas13a molecular detection platform,LwaCas13a was expressed in E.coli and its collateral RNase activity was determined.The recombinant plasmid pET His6-TwinStrep-SUMOLwaCas13a was transformed into Rosetta(DE3) competent cells to induce expression.The results of SDSPAGE electrophoresis and Western blot analysis showed that the soluble LwaCas13a recombinant protein was successfully expressed. Optimization of inducing temperature and time showed that maximal production of LwaCas13a was obtained at 15 h after induction at 16℃ .The expressed LwaCas13a recombinant protein was purified by nickel column affinity chromatography to obtain a single target band with ideal purification effect.In addition,the collateral RNase assay was also established with in vitro synthesized CRISPR RNA and target RNA.The assay could be performed within 30 min at constant 37℃ ,with the detection limit of 31.2 nmol/L.To sum up,the LwaCas13a was successfully expressed in E.coli Rosetta (DE3).Purified LwaCas13a exhibited ideal collateral RNase activity,and the collateral RNase assay for LwaCas13a was optimized and established.

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    Journal of Henan Agricultural Sciences    2008, 37 (3): 95-96.   DOI: 10.3969/j.issn.1004-3268.2008.03.028
    Abstract66)      PDF (74KB)(636)       Save
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    Application Progress of CRISPR/Cas9 Technology in Crop Genetic Breeding
    JIAO Yaolei, WANG Chunsheng, QU Shuo, SUN Shanshan, ZHU Tingting, ZHAO He, WANG Piwu
    Journal of Henan Agricultural Sciences    2021, 50 (7): 1-7.   DOI: 10.15933/j.cnki.1004-3268.2021.07.001
    Abstract727)      PDF (6994KB)(589)       Save
    CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISRP‑associated nuclease 9)is a new type of genome⁃directed editing technology after ZFNs(zinc finger nucleases)and TALENs(transcription activator like effector nucleases).Compared with the previous two generations of technology,it has the characteristics of simplicity and high efficiency.CRISPR/Cas9 is not only a basic research tool,but also has become one of currently useful molecular breeding tools,and important progress has been made in crop genetic improvement. The structure,classification,action mechanism of the CRISPR/Cas9 system and its application progress in crop quality improvement,yield enhancement, resistance breeding and male sterile material selection were reviewed,its existing problems were discussed,and its application prospects were prospected.

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    Journal of Henan Agricultural Sciences    2014, 43 (5): 114-117.  
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    Journal of Henan Agricultural Sciences    2014, 43 (3): 47-52.  
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    Journal of Henan Agricultural Sciences    2013, 42 (1): 106-109.  
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    Arsenic Content and Spatial Distribution in Paddy Soil Profile of Typical Parent Material in Hunan Province
    SHI Qiang, WANG Cuihong, BU Siyi, ZENG Ying, LI Xiaojing, OUYANG Ningxiang
    Journal of Henan Agricultural Sciences    2021, 50 (7): 87-100.   DOI: 10.15933/j.cnki.1004-3268.2021.07.011
    Abstract307)      PDF (13373KB)(572)       Save
    In order to guide the prevention and control of arsenic pollution in farmland and improve the basic database of heavy metals in farmland,the changes and spatial distribution characteristics of arsenic content in paddy soil profile of typical parent material in Hunan Province were studied. Based on the soil classification,59 typical sections and 332 soil samples were excavated from the paddy soils of Hunan typical parent materials,namely,alluvial sandy soil,grey clayey soil,yellow clayey soil,reddish yellow clayey soil,purple clayey soil and granitic sandy soil. The As content,pH,particle classification composition,organic matter and free iron oxide were detected and analyzed,with a focus on the section characteristics and spatial distribution of As in paddy soil and their relationship with physical and chemical properties of soil.The results showed that the content of total arsenic in the whole soil profile was 2.03—31.16 mg/kg,with an average of 12.30 mg/kg,mostly in the range of 2.00—18.00 mg/kg. The contents of total arsenic in alluvial sandy soil,grey clayey soil,yellow clayey soil,reddish yellow clayey soil,purple clayey soil and granitic sandy soil were 16.78,15.01,13.86,12.24,8.44,7.40 mg/kg. The arerage contents of total arsenic in the cultivated layer and the bottom layer were 12.09 mg/kg and12.34 mg/kg,respectively.On the whole,the contents of total arsenic in soils distributed in Eastern Hunan and in a few soil samples in Southern Hunan and Western Hunan were higher.The average contents of available arsenic in paddy soil were 0.19 mg/kg in the whole layer,0.26 mg/kg in the surface layer and 0.17 mg/kg in the bottom layer,respectively.The content of available arsenic was the highest in grey clayey soil,lowest in granitic sandy soil,and similar in other paddy soils.The contents of organic matter and free iron oxide in paddy soil were the main soil factors to determine the total arsenic content.In summary,most of the paddy soils tested in Hunan Province based on soil classification were at safe arsenic levels.
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    Journal of Henan Agricultural Sciences    2011, 40 (12): 20-24.  
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    Journal of Henan Agricultural Sciences    2007, 36 (10): 8-10,15.   DOI: 10.3969/j.issn.1004-3268.2007.10.002
    Abstract52)      PDF (145KB)(562)       Save
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    Journal of Henan Agricultural Sciences    2009, 38 (8): 101-104.   DOI: 10.3969/j.issn.1004-3268.2009.08.027
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    Journal of Henan Agricultural Sciences    2013, 42 (12): 11-15.  
    Abstract52)      PDF (226KB)(560)       Save
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    Research Progress of CTX-M Type Extended-spectrum β-lactamases
    LIU Baoguang, LI Yucheng, WANG Baoying, BAI Ming , MIAO Mingsan, XU Erping
    Journal of Henan Agricultural Sciences    2019, 48 (12): 1-7.   DOI: 10.15933/j.cnki.1004-3268.2019.12.001
    Abstract250)      PDF (1136KB)(559)       Save
    The production of extended-spectrum β-lactamases(ESBLs) is the main mechanism of bacterial resistance to β-lactamide antibiotics. In recent years,with the widespread use of cephalosporins,the spread of CTX-M ESBLs has become strong,causing popularity and spread in many countries and regions.It has gradually become the main type of ESBLs epidemic in many countries,and poses a great threat to human and animal health. In view of this,the discovery,species,biochemical characteristics,epidemiology and therapeutic measures of this enzyme strain in recent years are reviewed in this paper.The gene mutation,transfer,translocation factors and genetic environment of gene transfer of this enzyme strain are summarized,so as to provide references for the control of multidrug-resistant enzyme-producing bacteria.
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    Journal of Henan Agricultural Sciences    2015, 44 (2): 94-97.  
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    Journal of Henan Agricultural Sciences    2011, 40 (3): 19-24.  
    Abstract37)      PDF (511KB)(548)       Save
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    Journal of Henan Agricultural Sciences    2005, 34 (5): 30-32+45.   DOI: 10.3969/j.issn.1004-3268.2005.05.010
    Abstract48)      PDF (140KB)(547)       Save
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    Journal of Henan Agricultural Sciences    2011, 40 (12): 113-119.  
    Abstract42)      PDF (288KB)(546)       Save
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    Journal of Henan Agricultural Sciences    2004, 33 (1): 33-36.   DOI: 10.3969/j.issn.1004-3268.2004.01.011
    Abstract56)      PDF (128KB)(544)       Save
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    Journal of Henan Agricultural Sciences    2011, 40 (3): 125-128.  
    Abstract92)      PDF (143KB)(541)       Save
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    Journal of Henan Agricultural Sciences    2011, 40 (9): 30-33.  
    Abstract231)      PDF (199KB)(526)       Save
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    Journal of Henan Agricultural Sciences    2010, 39 (2): 113-114.   DOI: 10.3969/j.issn.1004-3268.2010.02.033
    Abstract58)      PDF (86KB)(526)       Save
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    Journal of Henan Agricultural Sciences    2011, 40 (10): 1-5.  
    Abstract33)      PDF (194KB)(522)       Save
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    Journal of Henan Agricultural Sciences    2010, 39 (7): 74-76.   DOI: 10.3969/j.issn.1004-3268.2010.07.021
    Abstract86)      PDF (209KB)(516)       Save
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    Journal of Henan Agricultural Sciences    2005, 34 (4): 16-21.   DOI: 10.3969/j.issn.1004-3268.2005.04.004
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    Journal of Henan Agricultural Sciences    2013, 42 (5): 1-4.  
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    Journal of Henan Agricultural Sciences    2019, 48 (5): 1-9.  
    Abstract545)      PDF (1051KB)(506)       Save
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    Journal of Henan Agricultural Sciences    2006, 35 (4): 9-12.   DOI: 10.3969/j.issn.1004-3268.2006.04.002
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    Journal of Henan Agricultural Sciences    2013, 42 (12): 162-164.  
    Abstract28)      PDF (144KB)(496)       Save
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    Journal of Henan Agricultural Sciences    2010, 39 (6): 150-155.   DOI: 10.3969/j.issn.1004-3268.2010.06.039
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    Journal of Henan Agricultural Sciences    2013, 42 (8): 162-166.  
    Abstract31)      PDF (809KB)(484)       Save
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    Journal of Henan Agricultural Sciences    2019, 48 (7): 1-5.  
    Abstract1222)      PDF (599KB)(476)       Save
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    Journal of Henan Agricultural Sciences    2010, 39 (2): 110-112.   DOI: 10.3969/j.issn.1004-3268.2010.02.032
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