Relationships between Leaf Structure of Tea Plants and Resistance to Tea Stick Thrip and Tea Green Leafhopper

doi:10.15933/j.cnki.1004-3268.2022.06.010

Abstract

Abstract: In order to identify the key structural factors affecting the insect resistance of tea tree varieties and quickly identify resistant varieties，12 tea varieties with different resistance were selected as experimental materials. Leaf physical structures were measured by paraffin section and scanning electron microscope.A total of 12 leaf anatomical structure traits and 13 leaf micro⁃morphological structure traits were analyzed. Relationship between leaf physical structures and the resistance of tea varieties to tea stick thrip（Dendrothrips minowai Prieser）and tea green leafhopper（Empoasca onukii Matsuda）was definited with correlation analysis.The results showed that thickness of upper epidermis，thickness of palisade tissue，density of leaf pubescences in highly resistant varieties were higher than those in highly susceptible varieties to D.minowai.But stomatal opening rate was lower than that in highly susceptible varieties.The number of calcium oxalate crystal in Fudingdabaicha was the lowest，which was a highly susceptible varietiy to E.onukii. Correlation analysis showed that stomatal density，density of leaf pubescences，density of calcium oxalate crystal and adaxial epidermal waxy ornamentation were significantly negatively correlated with the pest number ratio of D.minowai.Stomatal aperture and stomatal opening rate were significantly positively correlated with the pest number ratio of D.minowai.Whereas the inner stomatal width showed extremely significant positive correlation.The density of palisade tissue was significantly negatively correlated with the pest number ratio of E.onukii.No significant correlations were found for the other variables.Generally，the relationship between leaf structures and the resistance to D.minowai was stronger than that to E. onukii. Several leaf physical structures were significantly correlated with the resistance of tea varieties to D. minowai，particularly the stomata and pubescence.

Key words: Tea plant, Leaf structure, Resistance, Dendrothrips minowai Prieser, Empoasca onukii Matsuda

摘要： 为明确影响茶树品种抗虫性的叶片关键结构性状，快速鉴定茶树抗性品种，以12个不同抗性茶树品种为材料，采用石蜡切片和扫描电镜对叶片物理结构进行测量，结合相关系数分析12个叶片解剖结构性状和13个叶片微形态结构性状与茶树对茶棍蓟马和茶小绿叶蝉抗性的关系。结果表明，高抗茶棍蓟马茶树品种叶片上表皮厚度、栅栏组织厚度、叶片茸毛密度显著大于高感品种，气孔开放率显著小于高感品种；高感茶小绿叶蝉茶树品种福鼎大白茶叶片中草酸钙晶体数最少。相关性分析显示，气孔密度、叶片茸毛密度、草酸钙晶体密度、叶表蜡质纹饰形状与茶棍蓟马虫量比值呈显著负相关，气孔开度、气孔开放率与茶棍蓟马虫量比值呈显著正相关，内气孔宽与茶棍蓟马虫量比值呈极显著正相关；栅栏组织密度与茶小绿叶蝉虫量比值呈显著负相关；其他性状无显著相关性。综合来看，茶树叶片结构与茶树对茶棍蓟马的抗性关联强于茶树对茶小绿叶蝉的抗性关联，叶片多个物理性状与茶树对茶棍蓟马抗性显著相关，气孔和茸毛是决定茶树品种抗茶棍蓟马与否的关键。

关键词: 茶树, 叶片结构, 抗性, 茶棍蓟马, 茶小绿叶蝉

CLC Number:

S435.711

MENG Zehong, YANG Chun, LI Shuai, CHEN Zhengwu, ZHOU Yufeng. Relationships between Leaf Structure of Tea Plants and Resistance to Tea Stick Thrip and Tea Green Leafhopper[J]. Journal of Henan Agricultural Sciences, 2022, 51(6): 94-102.

孟泽洪, 杨春, 李帅, 陈正武, 周玉锋. 茶树对茶棍蓟马和茶小绿叶蝉抗性与叶片结构的关系[J]. 河南农业科学, 2022, 51(6): 94-102.

Figures/Tables 4

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