Distribution of Rice Blast Resistance Genes Pita and Pikm in Japonica Rice Germplasm Resources and Their Relationship with Rice Blast Resistance

doi:10.15933/j.cnki.1004-3268.2025.02.002

Abstract

Abstract: The blast resistance of 93 japonica rice germplasms，including local germplasms and introduced germplasms in Ningxia Hui Autonomous Region，was identified. Molecular detection of two rice blast resistance genes，Pita and Pikm，was conducted to analyze their genotypes. Subsequently，correlation analysis was performed between the comprehensive index of resistance and the resistance genes Pita and Pikm.The objective of this study was to provide a theoretical basis for rice blast⁃resistant breeding in Ningxia Hui Autonomous Region.The results showed that among the 93 germplasms，highly susceptible，moderately susceptible，susceptible，moderately resistant and resistant germplasms accounted for 2.2%（2/93），32.2%（30/93），6.5%（6/93），55.9%（52/93） and 3.2%（3/93），respectively.A total of 55 germplasms had resistance to rice blast，with resistance rate of 59.1%.Molecular detection results showed that there were 42 homozygotes for Pita resistance gene，accounting for 45.2% of the total germplasms.For Pikm resistance gene，there were 39 homozygotes，accounting for 41.9% of the total germplasms.The gene combination Pita⁺/Pikm⁺exhibited the best performance in enhancing resistance to rice blast，with an average comprehensive index of rice blast resistance of 2.63，followed by Pita⁺/Pikm^-and Pita^-/Pikm⁺，with average comprehensive indices of rice blast resistance of 4.08 and 4.42，respectively.In contrast，the resistance of gene combination Pita^-/Pikm^-was the weakest，with a comprehensive index of rice blast resistance of 5.33.The correlation analysis result showed that Pita and Pikm genes were significantly positively correlated with rice blast resistance，and there was also a significantly positive correlation between Pita and Pikm genes，indicating the presence of a synergistic effect of resistance between Pita and Pikm genes.Thus，integrating Pita and Pikm genes in molecular⁃assisted breeding programs against rice blast can improve the resistance to rice blast in Ningxia Hui Autonomous Region.

Key words: Rice blast, Pita, Pikm, Genotype, Resistance analysis

摘要： 对93份宁夏回族自治区当地及引进的粳稻种质进行稻瘟病抗性鉴定，并进行Pita、Pikm 2个抗稻瘟病基因的分子检测，分析其基因型，最后进行稻瘟病抗性综合指数与抗性基因Pita、Pikm 的相关性分析，以期为宁夏回族自治区水稻抗稻瘟病育种提供理论依据。结果表明，93份粳稻种质中，高感、中感、感病、中抗、抗病种质分别占2.2%（2/93）、32.2%（30/93）、6.5%（6/93）、55.9%（52/93）、3.2%（3/93），具有稻瘟病抗性的粳稻种质共有55份，抗病率为59.1%。分子检测结果显示，Pita抗性基因纯合体有42份，占总种质的45.2%；Pikm抗性基因纯合体有39份，占41.9%。基因组合Pita⁺/Pikm⁺在提高稻瘟病抗性方面表现最优秀，其稻瘟病抗性综合指数为2.63，其次是Pita⁺/Pikm^-、Pita^-/Pikm⁺，稻瘟病抗性综合指数分别为4.08、4.42，而Pita^-/Pikm^-的抗性最差（稻瘟病抗性综合指数为5.33）。相关性分析结果表明，Pita和Pikm基因均与粳稻种质稻瘟病抗性呈显著正相关，且Pita与Pikm基因也呈显著正相关，表明Pita和Pikm基因之间存在抗性叠加效应。综上，在生产上将Pita基因和Pikm基因相结合，用于抗稻瘟病的分子辅助聚合育种，可提高宁夏回族自治区水稻稻瘟病抗性。

关键词: 稻瘟病, Pita, Pikm, 基因型, 抗性分析

CLC Number:

S511

LIU Haolin, SHEN Wenjuan, GAO Yu, QI Jingyi, LIU Hairui, GUAN Ruijie, MA Yu, ZHANG Tianchang, ZHANG Yinxia. Distribution of Rice Blast Resistance Genes Pita and Pikm in Japonica Rice Germplasm Resources and Their Relationship with Rice Blast Resistance[J]. Journal of Henan Agricultural Sciences, 2025, 54(2): 10-20.

刘浩琳, 沈文娟, 高禹, 齐婧怡, 刘海瑞, 关睿捷, 马钰, 张恬畅, 张银霞. 粳稻种质资源抗稻瘟病基因Pita和Pikm的分布及与稻瘟病抗性的关系[J]. 河南农业科学, 2025, 54(2): 10-20.

Figures/Tables 11

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