Abstract: To precisely determine the resistance levels of Vigna radiata to imazethapyr and imazapic，explore the underlying molecular mechanisms，and assess potential cross‐resistance and multi‐resistance to other herbicides，the resistance levels of 27 V.radiata varieties to imazethapyr and imazapic were measured using the agar solid medium method.Additionally，the sensitive and resistant varieties were selected to evaluate their cross‐resistance and multi‐resistance to other herbicides. Finally，the mechanism through which V. radiata developed resistance to imidazolinone herbicides was elucidated via acetolactate synthase gene amplification and sequence alignment analysis.The results indicated that the preliminary screening results of resistance of 27 V.radiata varieties to imazethapyr revealed that seven varieties were sensitive，while five varieties were resistant，and one variety was suspected resistant.Both imazethapyr and imazapic could inhibit the growth and development of V.radiata.The resistance results of 13 V.radiata varieties to imazethapyr showed that seven varieties were identified as sensitive，accounting for 53.85% of the total，while six varieties exhibited low‐level resistance，accounting for 46.15%.Specifically，Jilü H1004 was the most sensitive variety，with a GR50 value of 18.80 mg/kg.Baolü 2016 22‐2 demonstrated the highest resistance，having a resistance index（RI）of 3.63.The resistance results of 13 V.radiata varieties to imazapic showed that seven varieties（53.85%）showed high resistance and five（38.46%）displayed extremely high resistance.Pinlü 2019‐26‐8‐17 had the highest resistance level，with a RI value of 237.85.The findings from the ALS gene sequence analysis indicated
that there were no disparities in the amino acid sequences of the two ALS genes between the relatively resistant and relatively sensitive V.radiata varieties.This suggested that the variation in the resistance of V.radiata to imidazolinone herbicides was not attributable to mutations at the ALS gene site.The results of the cross‐resistance analysis revealed that the relatively resistant cultivar Pinlü 2019‐26‐8‐17 exhibited cross‐resistance to choransulam‐methyl and halosulfuron‐methyl，with the RI values of 500.71 and 170.47，respectively.The results of the multi‐resistance analysis indicated that this relatively resistant cultivar demonstrated multi‐resistance to fluroxypyr，with a RI value of 4.38.However，it did not show multi‐resistance to bentazone，bixlozone and lactofen.In conclusion，among the tested varieties，there were variations in the resistance levels of different V.radiata varieties to imidazolinone herbicides.Moreover，the relative resistant cultivar exhibited multi‐resistance to fluroxypyr.These findings provide a
theoretical reference for effectively controlling weeds in V.radiata fields.

Key words: Vigna radiata, Resistance to herbicides, Imazethapyr, Imazapic, Cross‐resistance, Multi‐resistance

摘要： 为明确不同绿豆品种对2种咪唑啉酮除草剂（咪唑乙烟酸和甲咪唑烟酸）的抗药性水平及抗性机制，以及对另外6种除草剂的交互抗性与多抗性情况，室内利用琼脂固体培养基法测定了27个供试绿豆品种对咪唑乙烟酸和甲咪唑烟酸的抗药性水平，并选出敏感和抗性品种评估其对其他除草剂的交互抗性及多抗性情况，最后通过乙酰乳酸合成酶基因（ALS）扩增和序列比对，分析了绿豆对咪唑啉酮除草剂产生抗性的机制。结果表明，27个绿豆品种对咪唑乙烟酸抗性的初筛结果显示，敏感品种共7种，抗性品种共5种，疑似抗性品种1种。咪唑乙烟酸和甲咪唑烟酸对绿豆的生长发育均具有抑制作用。对筛选出的13个绿豆品种进行咪唑乙烟酸抗性分析，敏感品种7种，占比为53.85%，低水平抗性品种6种，占比为46.15%。其中，冀绿H1004最为敏感，抑制中量（GR₅₀）为18.80 mg/kg；保绿2016 22-2的抗性最高，抗性指数（RI）为3.63。对筛选出的13个绿豆品种进行甲咪唑烟酸抗性分析，敏感品种为保绿2016 10-3，GR₅₀值为3.21 mg/kg，高抗和极高抗品种分别为7种（53.85%）和5种（38.46%），其中，品绿2019-26-8-17的抗性水平最高，RI 为237.85。ALS 基因序列分析结果表明，相对抗性和相对敏感绿豆品种的2个ALS 基因的氨基酸序列无差异，表明绿豆对咪唑啉酮类除草剂的抗性差异不是由ALS 基因位点突变引起的。交互抗性分析结果表明，抗性品种品绿2019-26-8-17对氯酯磺草胺和氯吡嘧磺隆表现出明显的交互抗性，RI分别为500.71和170.47；对氯氟吡氧乙酸表现出多抗性，RI为4.38，而对灭草松、二氯异噁草酮和乳氟禾草灵未表现出多抗性。综上，供试绿豆品种对不同咪唑啉酮除草剂的抗性水平存在差异，且对氯氟吡氧乙酸表现出多抗性，为绿豆田杂草防除提供了理论参考。

关键词: 绿豆, 抗除草剂, 咪唑乙烟酸, 甲咪唑烟酸, 交互抗性, 多抗性