Research Progress on Application of CRISPR/Cas9 Gene Editing System in Wheat Breeding

doi:10.15933/j.cnki.1004-3268.2025.10.001

Journal of Henan Agricultural Sciences ›› 2025, Vol. 54 ›› Issue (10): 1-11.DOI: 10.15933/j.cnki.1004-3268.2025.10.001

• Special Subject on Wheat Breeding • Previous Articles Next Articles

Research Progress on Application of CRISPR/Cas9 Gene Editing System in Wheat Breeding

CHEN Yanyan¹，ZHAO Mingzhong¹，LI Yan^1,2，HUA Xia¹，FANG Yuhui^1,2，GONG Chen¹，QI Xueli^1,2

（1.Henan Academy of Crops Molecular Breeding，Zhengzhou 450002，China；2.The Shennong Laboratory，Zhengzhou 450002，China）

Received:2025-08-07 Accepted:2025-09-30 Published:2025-10-15 Online:2025-10-16

CRISPR/Cas9 基因编辑系统在小麦育种中的应用研究进展

陈艳艳¹，赵明忠¹，李艳^1,2，华夏¹，方宇辉^1,2，巩晨¹，齐学礼^1,2

（1.河南省作物分子育种研究院，河南郑州 450002；2.神农种业实验室，河南郑州 450002）

通讯作者: 齐学礼（1982-），男，河北晋州人，研究员，博士，主要从事小麦遗传育种研究。E-mail：xueliqi888@163.com。巩晨同为通信作者
作者简介:陈艳艳（1992-），女，河南郑州人，研究实习员，硕士，主要从事小麦遗传育种研究。E-mail：13140089598@163.com。赵明忠为同等贡献作者
基金资助:
国家小麦产业技术体系项目（GARS-3-7）；河南省农业科学院自主创新项目（2024ZC020）；河南省农业科学院基础性科研工作项目

Abstract

Abstract: Wheat is a crucial cereal crop，and the CRISPR/Cas9 gene editing system provides a powerful tool for wheat breeding. The principle of the CRISPR/Cas9 gene editing system was elaborated，its applications in enhancing wheat yield related traits，improving grain quality，increasing stress resistance（biotic and abiotic stresses），and accelerating the breeding process were reviewed，and the current challenges and future prospects were explored，providing a theoretical foundation for molecular breeding of wheat.

Key words: Wheat, Breeding, Gene editing, CRISPR/Cas9, Yield, Quality, Stress resistance

摘要： 小麦是重要的粮食作物，CRISPR/Cas9基因编辑系统为小麦育种提供了有力的工具。系统阐述了CRISPR/Cas9基因编辑系统的原理及其在小麦产量性状改良、品质改良、抗逆性（生物胁迫和非生物胁迫）提升、育种进程加速中的应用研究进展，并探讨了面临的挑战和未来发展前景，为小麦分子育种提供理论依据。

关键词: 小麦, 育种, 基因编辑, CRISPR/Cas9, 产量, 品质, 抗逆性

CLC Number:

S512

CHEN Yanyan1, ZHAO Mingzhong1, LI Yan1, 2, HUA Xia1, FANG Yuhui1, 2, GONG Chen1, QI Xueli1, 2. Research Progress on Application of CRISPR/Cas9 Gene Editing System in Wheat Breeding[J]. Journal of Henan Agricultural Sciences, 2025, 54(10): 1-11.

陈艳艳, 赵明忠, 李艳, 华夏, 方宇辉, 巩晨, 齐学礼. CRISPR/Cas9 基因编辑系统在小麦育种中的应用研究进展[J]. 河南农业科学, 2025, 54(10): 1-11.

[1]	ZHANG Suyu, LIU Hongjie, REN Dechao, YANG Mingda, GE Jun, ZHAO Jingling, ZHU Peipei, ZHENG Dongfang. Effects of Exogenous Regulators on Superior and Inferior Grain Filling and Yield of Wheat [J]. Journal of Henan Agricultural Sciences, 2025, 54(9): 23-33.
[2]	YAO Huina, LI Junfei, NIU Yingying, FENG Shuang, JIANG Yaling, LI Ke, GAO Weiwei. Agronomic Traits and Quality Differences of Different Provenances of Corydalis yanhusuo Planted in Henan Province [J]. Journal of Henan Agricultural Sciences, 2025, 54(9): 84-90.
[3]	YAO Danjun, LIAO Heng, ZHANG Qin, KUANG Shengjian, LIANG Yanfei, ZHANG Aihua, QIN Song, ZHU Qing. Effects of Different Green Manure Varieties and Sowing Times on the Growth，Soil Enzyme Activity，and Microbial Diversity of Chili Pepper in Guizhou [J]. Journal of Henan Agricultural Sciences, 2025, 54(9): 91-102.
[4]	WANG Haodong, LU Yifang, YANG Shan, LIU Fengying, WANG Gang. Effect of the gacS Gene in Pseudomonas chlororaphis on the Biocontrol Efficacy against Wheat Take‑all Disease [J]. Journal of Henan Agricultural Sciences, 2025, 54(9): 112-118.
[5]	CHEN Zhen, GUAN Qinglin, LIU Huabing, JIANG Jian, LU Xinbo, DONG Shuya, WU Yunjie, WU Jian. Quality Analysis of Heated Cigarette Raw Materials in Different Producing Areas Based on Metabonomics [J]. Journal of Henan Agricultural Sciences, 2025, 54(9): 159-170.
[6]	YANG Xin, LI Yanhong, NIE Jun, XIE Yuming, SHI Liangliang, ZHENG Jinrong. Effect of Different LED Light Formulations in Seedlings Raising on the Growth and Yield of Cherry Tomato [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 140-150.
[7]	LIU Xinglin, LIU Yuan, YANG Fan, LIU Buchun, HAN Rui. Research on County‑Level Yield Simulation of Winter Wheat in Henan Province Based on Machine Learning Algorithms [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 167-180.
[8]	XU Yi, CHEN Xiaoyang, NIU Jingwei, LIU Qing, JIANG Jinpeng. Research Progress on the Quantity，Quality and Ecological Regulation Technology of Damaged Arable Land in Coal Mining Areas of the Huaihe River Basin [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 1-14.
[9]	MA Shangying, XIA Tingting, HAN Pengbin, ZHANG Mengjiao, MAO Yingjie, WANG Zhiqiang, XIN Zeyu, LIN Tongbao, LIAN Yanhao, REN Yongzhe. Effects of Application Rate and Method of Nitrogen on Winter Wheat Nitrogen Uptake and Utilization，Yield and Soil Nitrate Nitrogen Residue under Wide‑Narrow Row Planting [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 38-50.
[10]	WANG Shujun, XING Lu. Analysis of Foxtail Millet Regional Trials Based on BLUP and GGE Biplot [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 51-59.
[11]	QIAN Jiancai, ZHU Yufei, DAN Dongchun, HUANG Wuxing, ZHANG Li. Effects of Different Ridging Time on Growth Period and Quality of Flue‑cured Tobacco in Luoyang Tobacco Growing Area [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 60-68.
[12]	JIN Jianghua, XIA Bing, LI Xu, YU Dapeng, GUO Wei, CHEN Tao, WU Wenxin, LI Sijun, WANG Xinyue, HUANG Jie, DENG Xiaohua. Fuzzy Comprehensive Evaluation of Leaf Quality and Economic Traits of Paddy‑Stubble Tobacco with Root Zone Application of Microbial Fertilizer [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 82-91.
[13]	CHENG Zhouqi, LIU Yuwu, ZHUO Le, QIU Lin, YI Zhenxie. Effects of Nitrogen‑Silicon Combined Application on Rice Planthopper Occurrence and Rice Yield in Double‑Cropping Rice Field [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 92-101.
[14]	GAO Xiaofeng, ZHANG Qiuyue, ZHOU Xiaojing, ZUO Weifang, HUANG Rantao, GUO Shuangshuang, YUAN Chaozheng, ZHENG Mingyan. Effects of EMS Mutagenesis on Seed Germination and Seedling Growth of Pomegranate [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 133-139.
[15]	LIU Qiuyuan, GONG Jingyang, WEI Yunfei, LI Meng, JI Xin, LIU Juan, FENG Fan, WANG Fujuan, LEI Zhenshan, SONG Xiaohua. Effects of Plant Spacing and Seedling Number per Hill Configurations on Panicle Traits，Yield and Quality of Japonica Rice [J]. Journal of Henan Agricultural Sciences, 2025, 54(7): 21-29.

Research Progress on Application of CRISPR/Cas9 Gene Editing System in Wheat Breeding

CRISPR/Cas9 基因编辑系统在小麦育种中的应用研究进展

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 1

References

Related Articles 15

Recommended Articles

Metrics