Abstract: To investigate the relationship between the metabolic products of flue‑cured tobacco leaves from different producing areas and the sensory quality of heated cigarettes，the heated cigarette roasted tobacco samples from eight producing areas in the United States（T1），Zimbabwe（T2），Brazil（T3），Yunnan Province（T4），Sichuan Province（T5），Guizhou Province（T6），Henan Province（T7）and Hunan Province（T8）in China were used as the research objects. The conventional chemical components of tobacco leaves from each producing area were detected，and the characteristics of metabolites were analyzed by metabolomics. The key differential metabolites were screened by least squares discriminant analysis and KEGG enrichment analysis，and their relationship with sensory quality was analyzed.The results showed that there were significant differences in metabolic characteristics of tobacco leaves from different producing areas. Compared with T4，T5 and T8，T1 tobacco leaves showed downregulation of cyclic adenosine monophosphate and guanine in purine metabolism pathway，and the sensory quality of heated cigarettes was the best. Compared with T4，T5 and T7，5‑hydroxyindole‑3‑acetic acid in tryptophan metabolism pathway was up‑regulated and irritation was the least in T2 tobacco leaves.Compared with T5 and T6，T3 tobacco leaves were up‑regulated in arginine and proline metabolism pathways，such as γ‑aminobutyric acid. The ratio of two sugars was the highest，the ratio of sugar to alkali was the lowest，and the irritation of heated cigarettes was larger. Compared with T6 and T8，L‑aspartic acid was up‑regulated in the alanine，aspartate and glutamate metabolism pathway of T4 tobacco leaves，and the sugar‑alkali ratio was the highest.The smoke temperature and aroma scores of heated cigarettes were lower than those of T8. Compared with T6 and T8，2´，4´，6´，3，4‑pentahydroxychalcone and dihydroquercetin were down‑regulated in flavonoid biosynthesis pathway of T5 tobacco leaves，and the ratio of potassium to chlorine was high but the harmony was poor. Compared with T1，T2，T4，T5 and T6，many metabolites in β‑alanine metabolism pathway of T7 tobacco leaves were significantly up‑regulated.Compared with T2，T3，T4 and T6，lysine biosynthesis pathway in T7 tobacco leaves was also different.The ratio of potassium to chlorine was low，and the irritation and offensive odor of heated cigarettes were more. Correlation analysis showed that 5‑hydroxyindole‑3‑acetic acid and other compounds were significantly correlated with the sensory quality of heated cigarettes. In summary，T1 tobacco leaves are most suitable for heating cigarettes，followed by T8，and T7 has poor quality.

Key words: Heated cigarette, Producing area, Chemical composition, Differential metabolite, Sensory quality

摘要： 为研究不同产区烤烟烟叶的代谢产物与制成的加热卷烟感官品质的关系，以美国（T1）、津巴布韦（T2）、巴西（T3），中国云南省（T4）、四川省（T5）、贵州省（T6）、河南省（T7）、湖南省（T8）8个产区的加热卷烟烤后烟叶样品为研究对象，检测各产区烟叶的常规化学成分，采用代谢组学技术分析代谢产物特征，通过最小二乘判别分析和KEGG富集分析筛选关键差异代谢物，并解析其与感官品质的关系。结果表明，不同产区烟叶在代谢特征上存在显著差异。T1烟叶与T4、T5、T8相比在嘌呤代谢通路中环磷酸腺苷、鸟嘌呤等下调，加热卷烟感官质量最好；T2烟叶与T4、T5、T7相比，在色氨酸代谢通路中的5-羟基吲哚乙酸上调，刺激性最少；T3烟叶与T5、T6相比，在精氨酸和脯氨酸代谢通路中4-氨基丁酸上调，两糖比最高，糖碱比最低，加热卷烟刺激性较大；T4烟叶与T6、T8相比，在丙氨酸、天冬氨酸和谷氨酸代谢通路中L-天冬氨酸上调，糖碱比最高，加热卷烟烟气温度和香气得分低于T8；T5烟叶和T6、T8相比，在类黄酮生物合成通路中2´，4´，6´，3，4-五羟查尔酮、二氢槲皮素下调，钾氯比高但谐调差；T7烟叶与T1、T2、T4、T5、T6相比，在β-丙氨酸代谢通路中多种代谢物显著上调，与T2、T3、T4、T6相比，在赖氨酸生物合成通路中也存在差异，其钾氯比低，加热卷烟的刺激性和杂气较多。相关性分析表明，5-羟基吲哚乙酸等与加热卷烟感官质量显著相关。综上，T1烟叶最适用于加热卷烟，T8次之，T7品质较差。

关键词: 加热卷烟, 产区, 化学成分, 差异代谢物, 感官品质