Identification and Evaluation of Resistance to Stripe Rust of 480 Wheat Germplasms

doi:10.15933/j.cnki.1004-3268.2024.09.001

Abstract

Abstract: The main purpose of the present study is to understand the resistance to stripe rust and the utilization of stripe rust resistant genes in current bred wheat varieties,and find the varieties containing novel stripe rust resistant loci.Totally,480 domestic and foreign wheat germplasms were collected from various planting regions.The varieties were inoculated with mixed races of CYR32,CYR33 and CYR34 for testing the adult plant resistance.Simultaneously,the resistance to leaf rust and powdery mildew was investigated in infected field.The KASP markers closely linked or co‑segregated with stripe rust,leaf rust and powdery mildew genes,Yr18/Lr34/Sr57/Pm38(7DS),Yr29/Lr46/Pm39/Sr58(1BL)and Yr30/Lr27/Sr2/Sb3(3BS),were selected for identifying the previous reported pleiotropic resistant genes.The results showed that there were 35(accounting for 7.29%)germplasms showed immunization to CYR32,CYR33 and CYR34,while the numbers of high‑resistant and medium‑resistant germplasms were 69(14.38%)and 79(16.46%),respectively.And the other 297(61.88%)germplasms demonstrated medium‑ or high‑ susceptible to the inoculated races.There were 13,10 and 7 germplasms containing the Yr18,and Yr29 and Yr30 genes,respectively;only 3 germplasms contained two of the resistant genes;no germplasm was detected with all the three resistant genes.In conclusion,183(accounting for 38.12%)germplasms showed resistant to stripe rust at adult plant stage,and 27 germplasms were detected with the above resistant genes.Yr18 or Yr30 gene showed resistant to the current races of stripe rust,however,the resistance level of Yr29 gene was partly lost at adult plant stage.

Key words: Wheat, Stripe rust, Adult plant resistance, Resistance identification, Pleiotropic disease resistant gene, KASP detection

摘要： 为了解现有小麦品种（系）对条锈病的抗性水平和条锈病抗性基因的利用情况，寻找新的条锈病抗源，收集不同生态区小麦品种（系）480份，田间接种当前流行生理小种（CYR32、CYR33和CYR34）混合菌种进行成株期条锈病抗性鉴定，同时调查叶锈病和白粉病田间自然发病情况，并利用已知小麦多效抗病基因位点Yr18/Lr34/Sr57/Pm38（7DS）、Yr29/Lr46/Pm39/Sr58（1BL）和Yr30/Lr27/Sr2/Sb3（3BS）的KASP标记进行抗条锈病基因检测。结果表明，供试的小麦种质中，有35份种质接种后对条锈病表现免疫，占参试种质的7.29%；69份种质表现高抗，占14.38%；79份种质表现中抗，占16.46%；其他297份种质表现中感或高感，占61.88%；含有Yr18 基因抗性条带的种质有13份，含有Yr29 基因抗性条带的种质有10份，含有Yr30 基因抗性条带的种质有7份，同时携带2个抗性基因的种质有3份，没有同时携带3个抗性基因的种质。综上，480份小麦种质成株期条锈病抗性表现为抗病的有183份，占比38.12%，携带多效抗病基因的种质27份。携带Yr18 或Yr30 基因的种质对现有条锈病生理小种具有抗性，而Yr29 基因的抗性水平已部分丧失。

关键词: 小麦, 条锈病, 成株期抗性, 抗性鉴定, 多效抗病基因, KASP标记检测

CLC Number:

S512

DAI Ziju, LI Wenxu, YANG Huimin, ZHU Xiuhua, WANG Yahuan, XU Fuxin, LIU Dongyang, HOU Jinna, QIN Maomao, WU Zhengqing, ZHOU Zhengfu, LEI Zhensheng. Identification and Evaluation of Resistance to Stripe Rust of 480 Wheat Germplasms[J]. Journal of Henan Agricultural Sciences, 2024, 53(9): 1-15.

代资举, 李文旭, 杨会民, 朱秀华, 王亚欢, 徐福新, 柳东阳, 侯锦娜, 秦毛毛, 吴政卿, 周正富, 雷振生. 480 份小麦种质条锈病抗性鉴定与评价[J]. 河南农业科学, 2024, 53(9): 1-15.

References

［1］CHEN X M.Pathogens which threaten food security：Puccinia striiformis，the wheat stripe rust pathogen［J］.Food Security，2020，12（2）：239‑251.

［2］陈万权，康振生，马占鸿，等.中国小麦条锈病综合治理理论与实践［J］.中国农业科学，2013，46（20）：4254‑4262.

CHEN W Q，KANG Z S，MA Z H，et al.Integrated management of wheat stripe rust caused by Puccinia striiformis f.sp.tritici in China［J］.Scientia Agricultura Sinica，2013，46（20）：4254‑4262.
［3］康振生，王晓杰，赵杰，等.小麦条锈菌致病性及其变异研究进展［J］.中国农业科学，2015，48（17）：3439‑3453.

KANG Z S，WANG X J，ZHAO J，et al.Advances in research of pathogenicity and virulence variation of the wheat stripe rust fungus Puccinia striiformis f.sp.tritici［J］.Scientia Agricultura Sinica，2015，48 （17）：3439‑3453.

［4］贾秋珍，黄瑾，曹世勤，等.感染我国重要小麦抗源材料贵农22的条锈菌新菌系的发现及致病性初步分析［J］.甘肃农业科技，2012（1）：3‑5.

JIA Q Z，HUANG J，CAO S Q，et al.Discovery of new stripe rust strain infected to China’s major wheat resistant material Guinong 22 and its preliminary pathogenicity analysis［J］.Gansu Agricultural Science and Technology，2012（1）：3‑5.

［5］刘博，刘太国，章振羽，等.中国小麦条锈菌条中34号的发现及其致病特性［J］.植物病理学报，2017，47（5）：681‑687.

LIU B，LIU T G，ZHANG Z Y，et al.Discovery and pathogenicity of CYR34，a new race of Puccinia striiformis f.sp.triticiin in China［J］.Acta Phytopathologica Sinica，2017，47（5）：681‑687.

［6］魏国荣，韩德俊，赵杰，等.小麦成株期抗条锈病种质筛选与评价［J］.麦类作物学报，2011，31（2）：376‑381.

WEI G R，HAN D J，ZHAO J，et al.Identification and evaluation of adult plant resistance to stripe rust in wheat

germplasms［J］.Journal of Triticeae Crops，2011，31（2）：376‑381.

［7］LI J B，DUNDAS I，DONG C M，et al.Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat［J］.Theoretical and Applied Genetics，2020，133（4）：1095‑1107.

［8］JAMIL S，SHAHZAD R，AHMAD S，et al.Role of genetics，genomics，and breeding approaches to combat stripe rust of wheat［J］.Frontiers in Nutrition，2020，7：580715.

［9］MOORE J W，HERRERA‑FOESSEL S，LAN C X，et al.A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat［J］.Nature Genetics，2015，47（12）：1494‑1498.

［10］HERRERA‑FOESSEL S A，SINGH R P，LILLEMO M，et al.Lr67/Yr46 confers adult plant resistance to stem

rust and powdery mildew in wheat［J］.Theoretical and Applied Genetics，2014，127（4）：781‑789.

［11］刘金栋，杨恩年，肖永贵，等.兼抗型成株抗性小麦品系的培育、鉴定与分子检测［J］.作物学报，2015，41 （10）：1472‑1480.

LIU J D，YANG E N，XIAO Y G，et al.Development，field and molecular characterization of advanced lines with pleiotropic adult‑plant resistance in common wheat［J］.Acta Agronomica Sinica，2015，41（10）：1472‑1480.

［12］方桃红，张敏，马春花，等. 小麦抗条锈基因Yr52在品种改良中的应用［J］.中国农业科学，2022，55（11）：2077‑2091.

FANG T H，ZHANG M，MA C H，et al.Application of Yr52 gene in wheat improvement for stripe rust resistance［J］.Scientia Agricultura Sinica，2022，55 （11）：2077‑2091.

［13］徐默然，蔺瑞明，王凤涛，等.103份小麦品种（系）抗条锈性和遗传多样性评价及基因检测［J］.中国农业科学，2020，53（4）：748‑760.

XU M R，LIN R M，WANG F T，et al.Evaluation of resistance to stripe rust and genetic diversity and detection of resistance genes in 103 wheat cultivars（lines）［J］.Scientia Agricultura Sinica，2020，53（4）：748‑760.

［14］KRATTINGER S G，JORDAN D R，MACE E S，et al.Recent emergence of the wheat Lr34 multi‑pathogen resistance：Insights from haplotype analysis in wheat，rice，sorghum and Aegilops tauschii［J］.Theoretical and Applied Genetics，2013，126（3）：663‑672.

［15］LAGUDAH E S，KRATTINGER S G，HERRERAFOESSEL S，et al.Gene‑specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens［J］.Theoretical and Applied Genetics，2009，119（5）：889‑898.

［16］李玮，宋国琦，李吉虎，等.小麦四个多效抗病基因的分子检测［J］.麦类作物学报，2020，40（4）：395‑400.
LI W，SONG G Q，LI J H，et al.Molecular detection of four pleiotropic disease resistance genes in wheat［J］.Journal of Triticeae Crops，2020，40（4）：395‑400.

［17］KRATTINGER S G，LAGUDAH E S，WICKER T，et al.Lr34 multi‑pathogen resistance ABC transporter：Molecular analysis of homoeologous and orthologous genes in hexaploid wheat and other grass species［J］.The Plant Journal，2011，65（3）：392‑403.

［18］RISK J M，SELTER L L，CHAUHAN H，et al.The wheat Lr34 gene provides resistance against multiple fungal pathogens in barley［J］.Plant Biotechnology Journal，2013，11（7）：847‑854.

［19］KRATTINGER S G，SUCHER J，SELTER L L，et al.The wheat durable，multipathogen resistance gene Lr34 confers partial blast resistance in rice［J］.Plant Biotechnology Journal，2016，14（5）：1261‑1268.

［20］SCHNIPPENKOETTER W，LO C，LIU G Q，et al.The wheat Lr34 multipathogen resistance gene confers resistance to anthracnose and rust in sorghum［J］.Plant Biotechnology Journal，2017，15（11）：1387‑1396.

［21］SUCHER J，BONI R，YANG P，et al.The durable wheat disease resistance gene Lr34 confers common rust and northern corn leaf blight resistance in maize［J］.Plant Biotechnology Journal，2017，15（4）：489‑496.

［22］COBO N，WANJUGI H，LAGUDAH E，et al.A high‑resolution map of wheat QYr.ucw‑1BL，an adult plant stripe rust resistance locus in the same chromosomal region as Yr29［J］.Plant Genome，2019，12（1）：1‑15.

［23］MAGO R，TABE L，VAUTRIN S，et al.Major haplotype divergence including multiple germin‑like protein genes，at the wheat Sr2 adult plant stem rust resistance locus［J］.BMC Plant Biology，2014，14（5）：379.

［24］周新力，詹刚明，黄丽丽，等.80份国外春小麦种质资源抗条锈性评价［J］.中国农业科学，2015，48（8）：1518‑1526.
ZHOU X L，ZHAN G M，HUANG L L，et al.Evaluation of resistance to stripe rust in eighty abroad spring wheat germplasms［J］.Scientia Agricultura Sinica，2015，48（8）：1518‑1526.

［25］MAKHOUL M，RAMBLA C，VOSS‑FELS K P，et al.Overcoming polyploidy pitfalls：A user guide for effective SNP conversion into KASP markers in wheat ［J］.Theoretical and Applied Genetics，2020 133（8）：2413‑2430.

［26］高洁，宋国琦，李吉虎，等.小麦4个多效抗病基因分子标记的转化和再开发［J］.农业生物技术学报，2021，29（5）：847‑856.
GAO J，SONG G Q，LI J H，et al.Convertion and redevelopment of molecular markers of 4 pleiotropic disease resistance genes in wheat（Triticum aestivum）［J］.Journal of Agricultural Biotechnology，2021，29 （5）：847‑856.

［27］MOOSE S P，MUMM R H.Molecular plant breeding as the foundation for 21st century crop improvement［J］.Plant Physiology，2008，147（3）：969‑977.
［28］ALLEN G C，FLORES‑VERGARA M A，KRASYNANSKI S，et al.A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide［J］.Nature Protocols，2006，1（5）：2320‑2325.

［29］RASHEED A，WEN W E，GAO F M，et al.Development and validation of KASP assays for genes underpinning key economic traits in bread wheat［J］.Theoretical and Applied Genetics，2016，129（10）：1843‑1860.

［30］姚强.120个春小麦品种（系）成株期抗条锈性鉴定与评价［J］.西北农业学报，2014，23（7）：96‑101.
YAO Q.Identification and evaluation of adult‑plant resistance to stripe rust in 120 spring wheat varieties （lines）［J］.Acta Agriculturae Boreali‑Occidentalis Sinica，2014，23（7）：96‑101.

［31］董娜，陈向东，胡铁柱，等.39份外引小麦种质抗病基因的分子标记检测及其抗病性评价［J］.华北农学报，2018，33（6）：49‑55.
DONG N，CHEN X D，HU T Z，et al.Molecular detection and evaluation of disease resistance genes of 39 introduced wheat germplasms［J］.Acta Agriculturae Boreali‑Sinica，2018，33（6）：49‑55.

［32］王鑫，宋鹏博，王笑笑，等.305份国内外小麦种质条锈病与白粉病抗性鉴定与评价［J］.麦类作物学报，2021，41（6）：689‑698.
WANG X，SONG P B，WANG X X，et al.Identification and evaluation of resistance to stripe rust and powdery mildew of 305 domestic and foreign wheat germplasms［J］.Journal of Triticeae Crops，2021，41（6）：689‑698.

［33］张海鹏，叶雪玲，管方念，等.220份四川小麦条锈病抗性鉴定与评价［J］.四川农业大学学报，2023，41（6）：1020‑1031.
ZHANG H P，YE X L，GUAN F N，et al.Identification and evaluation of stripe rust resistance in 220 Sichuan wheat germplasms［J］.Journal of Sichuan Agricultural University，2023，41（6）：1020‑1031.

［34］周警卫，叶博伟，张朋飞，等.国内外153份小麦种质条锈病抗性鉴定与评价［J］.中国农业科学，2024，57 （1）：18‑33.
ZHOU J W，YE B W，ZHANG P F，et al.Identification and evaluation of stripe rust resistance in 153 wheat collections［J］.Scientia Agricultura Sinica，2024，57 （1）：18‑33.

［35］ZHANG P P，YAN X C，GEBREWAHID T W，et al.Genome‑wide association mapping of leaf rust and stripe rust resistance in wheat accessions using the 90K SNP array［J］.Theoretical and Applied Genetics，2021，134（4）：1233‑1251.

［36］HU J H，LI J T，WU P P，et al.Development of SNP，KASP，and SSR markers by BSR‑seq technology for saturation of genetic linkage map and efficient detection of wheat powdery mildew resistance gene Pm61［J］.International Journal of Molecular Sciences，2019，20（3）：750.

［37］延荣，耿妙苗，李晓静，等.河北省小麦品种和种质资源抗白粉病鉴定与抗病基因分子标记检测［J］.植物遗传资源学报，2020，21（3）：683‑705.
YAN R，GENG M M，LI X J，et al.Phenotyping and marker‑assisted gene identification of powdery mildew resistance in wheat commercial varieties and germplasm resources from Hebei Province［J］.Journal of Plant Genetic Resources，2020，21（3）：683‑705.

［38］李顺达，兰彩霞.小麦抗条锈病和白粉病的遗传及育种［J］.中国农业科学，2024，57（1）：1‑3.
LI S D，LAN C X.Genetic inheritance and breeding of stripe rust and powdery mildew resistance in wheat［J］.Scientia Agricultura Sinica，2024，57（1）：1‑3.

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