Effect of Halloysite Nanotubes on Growth of Wheat under Copper Stress

doi:10.15933/j.cnki.1004-3268.2024.07.004

Abstract

Abstract: Three copper（Cu）treatments［20 mg/L Cu²⁺（20Cu），50 mg/L Cu²⁺（50Cu）and 100 mg/L Cu²⁺（100Cu）］were set up under hydroponics to study the effect of exogenous addition of 50 mg/L halloysite nanotubes（HNTs）on wheat seedling growth，stomata size，chloroplast pigment content，oxidative damage of roots and Cu²⁺ content in plants under Cu stress，so as to provide theoretical basis for HNTs to alleviate the effects of heavy metal pollution on crop growth.The results showed that exogenous addition of HNTs significantly increased the plant height and root length of wheat seedlings under Cu stress，the plant height of 20Cu+HNTs，50Cu+HNTs and 100Cu+HNTs treatments increased by 13.10%，26.45% and 28.30%，and the root length increased by 62.87%，56.68% and 92.34%，respectively.Exogenous addition of HNTs significantly increased the dry and fresh weight of wheat seedlings，chlorophyll and carotenoid contents of wheat seedlings under Cu stress，as well as root activity，and reduced the oxidative damage degree of roots，among which chlorophyll a content increased by 34.34%，18.09% and 54.76%，chlorophyll b content increased by 44. 83%，25. 93% and 43. 75%，and root activity increased by 38.16%，339.48% and 453.56%，respectively.Compared with 100Cu treatment，the Cu²⁺ content in roots and leaves of wheat seedlings of 100Cu+HNTs treatment decreased by 19.18% and 37.02%，respectively.In conclusion，exogenous addition of HNTs can reduce Cu²⁺ content in wheat seedlings，alleviate the toxicity of heavy metal Cu to wheat seedlings，and promote their growth.

Key words: Wheat, Halloysite nanotubes, Copper stress, Growth and development, Mitigation

摘要： 在水培条件下设置3个铜（Cu）处理［20 mg/L Cu²⁺（20Cu）、50 mg/L Cu²⁺（50Cu）和100 mg/L Cu²⁺（100Cu）］，研究外源添加50 mg/L埃洛石纳米管（HNTs）对Cu胁迫下小麦幼苗生长、气孔大小、叶绿体色素含量、根系氧化损伤、植株内Cu²⁺含量的影响，以期为HNTs缓解重金属污染对作物生长的影响提供理论依据。结果表明，外源添加HNTs显著提高了Cu胁迫下小麦幼苗的株高、根长，20Cu+HNTs、50Cu+HNTs、100Cu+HNTs 处理株高分别提高了13.10%、26.45%、28.30%，根长分别提高了62.87%、56.68%、92.34%；外源添加HNTs显著提高了Cu胁迫下小麦幼苗干、鲜质量，叶绿素、类胡萝卜素含量，根系活力，降低了根系氧化损伤程度，其中，叶绿素a含量分别提高了34.34%、18.09%、54.76%，叶绿素b含量分别提高了44.83%、25.93%、43.75%，根系活力分别提高了38.16%、339.48%、453.56%；与100Cu 处理相比，100Cu+HNTs 处理小麦幼苗根系、叶片中Cu²⁺含量分别降低了19.18%、37.02%。综上，外源添加HNTs能够降低小麦幼苗中Cu2+含量，缓解重金属Cu对小麦幼苗的毒性，促进其生长。

关键词: 小麦, 埃洛石纳米管, 铜胁迫, 生长发育, 缓解

CLC Number:

S512

WANG Jingying, WANG Jiaxu, CHEN Lina, WANG Xiuping. Effect of Halloysite Nanotubes on Growth of Wheat under Copper Stress[J]. Journal of Henan Agricultural Sciences, 2024, 53(7): 28-34.

王敬营, 王佳旭, 陈丽娜, 王秀平. 埃洛石纳米管对铜胁迫下小麦幼苗生长的影响[J]. 河南农业科学, 2024, 53(7): 28-34.

References

［1］ALHAMMAD B A，SELEIMAN M F，HARRISON M T.Hydrogen peroxide mitigates Cu stress in wheat［J］.Agriculture，2023，13（4）：862.

［2］XU J H，HU C Y，WANG M L，et al.Changeable effects of coexisting heavy metals on transfer of cadmium from soils to wheat grains［J］.Journal of Hazardous Materials， 2022，423：127182.

［3］SINGH S，PARIHAR P，SINGH R，et al.Heavy metal tolerance in plants：Role of transcriptomics，proteomics， metabolomics，and ionomics［J］.Front Plant Sci，2016，6：1143.

［4］RIZVI A，ZAIDI A，AMEEN F，et al. Heavy metal induced stress on wheat：Phytotoxicity and microbiological management［J］.RSC Advances，2020， 10：38379-38403.

［5］BARUAH N，MONDAL S C，FAROOQ M，et al.Influence of heavy metals on seed germination and seedling growth of wheat，pea，and tomato［J］.Water， Air，& Soil Pollution，2019，230（12）：273.

［6］LIU P，LI L，IPPOLITO J A，et al.Heavy metal distribution in wheat plant components following foliar Cd application［J］.Chemosphere，2023，322：138177.

［7］NIYOIFASHA C J，BORENA B M，UKOB I T，et al.Alleviation of Hg-，Cr-，Cu-，and Zn- induced heavy metals stress by exogenous sodium nitroprusside in rice plants［J］.Plants，2023，12（6）：1299.

［8］HABIBI R， BAKHTIARI O.Preparation and characterization of modified halloysite nanotubes：Pebax nanocomposite membranes for CO2 /CH4 separation［J］.Chemical Engineering Research and Design，2021，174： 199-212.

［9］GANAPATHY D，SHANMUGAM R，PITCHIAH S，et al.Potential applications of halloysite nanotubes as drug carriers：A review［J］.Journal of Nanomaterials，2022， 2022：1068536.

［10］LIU M X，JIA Z X，JIA D M，et al.Recent advance in research on halloysite nanotubes-polymer nanocomposite［J］.Progress in Polymer Science，2014，39（8）：1498-1525.

［11］ZENG X Q，ZHONG B C，JIA Z X，et al.Halloysite nanotubes as nanocarriers for plant herbicide and its controlled release in biodegradable polymers composite film［J］.Applied Clay Science，2019，171：20-28.

[12］赵亚婔.埃洛石纳米管及其改性产品在废水处理中的应用研究［D］.郑州：郑州大学，2010. ZHAO Y F. Study the application of hollaysite nanotubes and modified hollaysite nanotubes in the wastewater treatment［D］.Zhengzhou：Zhengzhou University，2010.

［13］刘琳.海藻酸盐/埃洛石纳米管复合材料的制备及吸附性能的研究［D］.郑州：郑州大学，2012.

LIU L.Study on the preparation and adsorption properities of the alginate/halloysite nanotubes hybrid materials［D］.Zhengzhou：Zhengzhou University，2012.

［14］BELLANI L，GIORGETTI L，RIELA S，et al.Ecotoxicity of halloysite nanotube-supported palladium nanoparticles in Raphanus sativus L［J］.Environmental Toxicology and Chemistry，2016，35（10）：2503-2510.

［15］KURCZEWSKA J，CEGŁOWSKI M，MESSYASZ B，et al.Dendrimer-functionalized halloysite nanotubes for effective drug delivery［J］.Applied Clay Science，2018， 153：134-143.

［16］WAHID I，RANI P，KUMARI S，et al.Biosynthesized gold nanoparticles maintained nitrogen metabolism，nitric oxide synthesis，ions balance，and stabilizes the defense systems to improve salt stress tolerance in wheat［J］.Chemosphere，2022，287（Pt 2）：132142.

［17］IBRAHIM M，NAWAZ S，IQBAL K，et al.Plant-derived smoke solution alleviates cellular oxidative stress caused by arsenic and mercury by modulating the cellular antioxidative defense system in wheat［J］.Plants，2022， 11（10）：1379.

［18］SHAH F A，NI J，TANG C G，et al.Karrikinolide alleviates salt stress in wheat by regulating the redox and K+/Na+ homeostasis［J］.Plant Physiology and Biochemistry，2021，167：921-933.

［19］朱秀云，梁梦，马玉.根系活力的测定（TTC法）实验综述报告［J］.广东化工，2020，47（6）：211-212.

ZHU X Y，LIANG M，MA Y.A review report on the experiments for the determination of root activity by TTC method［J］.Guangdong Chemical Industry，2020， 47（6）：211-212.

［20］潘传荣，黄玉，古汶玉，等.大米中镉含量测定的湿式消解工艺优化［J］.粮食科技与经济，2021，46（4）：96-99. PAN C R，HUANG Y，GU W Y，et al.Rapid optimization of wet digestion method for determination of cadmium in rice［J］.Grain Science and Technology and Economy，2021，46（4）：96-99.

［21］吕笃康，巴音山，赵玉.铜、镉污染对小麦种子萌发及幼苗生长的影响［J］.种子，2012，31（ 9）：108-111.

LÜ D K，BA Y S，ZHAO Y.Effects of copper and cadmium pollution on wheat seed germination and seedling growth［J］.Seed，2012，31（ 9）：108-111.

［22］PRINZ SETTER O，SEGAL E.Halloysite nanotubes：The nano-bio interface［J］.Nanoscale，2020，12（46）：23444-23460.

［23］唐凝，王华瑞，裴冬丽.外源亚精胺对铜离子胁迫下小麦幼苗生长及抗氧化酶的影响［J］.东北农业科学， 2022，47（1）：22-25.

TANG N，WANG H R，PEI D L.Effects of exogenous spd on the growth and antioxidant enzyme system of wheat seedlings under Cu2+ stress［J］.Journal of Northeast Agricultural Sciences，2022，47（1）：22-25.

［24］RIZVI A，AHMED B，ZAIDI A，et al.Heavy metal mediated phytotoxic impact on winter wheat：Oxidative stress and microbial management of toxicity by Bacillus subtilis BM2［J］.RSC Advances，2019，9（11）： 6125-6142.

［25］SANJOSÉ I，NAVARRO-ROLDÁN F，INFANTE IZQUIERDO M D，et al.Accumulation and effect of heavy metals on the germination and growth of Salsola vermiculata L.seedlings［J］.Diversity，2021，13（11）： 539.

［26］JAŃCZAK-PIENIĄŻEK M，CICHOŃSKI J，MICHALIK P，et al.Effect of heavy metal stress on phenolic compounds accumulation in winter wheat plants［J］.Molecules，2022，28（1）：241.

［27］CAVALLARO G，LAZZARA G，ROZHINA E，et al.Organic-nanoclay composite materials as removal agents for environmental decontamination［J］.RSC Advances，2019，9：40553-40564.

［28］BIDDECI G，SPINELLI G，COLOMBA P，et al.Nanomaterials：A review about halloysite nanotubes， properties，and application in the biological field［J］.International Journal of Molecular Sciences，2022，23 （19）：11518.

［29］NAZIR A，RAFIQUE F，AHMED K，et al.Evaluation of heavy metals effects on morpho-anatomical alterations of wheat（Triticum aestivum L.）seedlings［J］.Microscopy Research and Technique，2021，84 （11）：2517-2529.

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