Abstract: Taking Jimai 22 and Bainong 307 as materials，the effects of plasma⁃activated water（PAW，with preparation time of 5.0，7.5 and 10 min，named as PAW⁃5，PAW⁃7.5 and PAW⁃10，respectively）and its combined action with low⁃temperature plasma（LTP，named as LTP+W⁃5，LTP+W⁃7.5，LTP+W⁃10，respectively）on wheat seed germination and seedling growth were studied，so as to provide atheoretical basis for the application of PAW and LTP in wheat seed vigor enhancement.The results showed that the germination rate and germination index of two wheat varieties reached the best at 9 s after LTP treatment，so LTP treatment time of 9 s was chosen for the study of the combined effect of PAW and LTP.The PAW treatment and the combined treatment of PAW and LTP increased the germination rate of wheat seeds，plant height，fresh and dry weight of seedlings，and significantly increased seed vigor index，and LTP+W⁃7.5 treatment was generally the best.The contact angle between seeds without LTP treatment and distilled water was the largest，indicating that the wettability was the weakest. In contrast，the contact angle between seeds without LTP treatment and PAW decreased，while the contact angle between seeds with LTP treatment and distilled water/PAW significantly decreased. Compared with treatment treated with distilled water，the content of reactive oxygen and nitrogen species（RONS）in wheat seedlings treated with PAW and PAW+LTP generally significantly increased，while the RONS content in wheat seedlings treated only with LTP was not significantly changed.In conclusion，PAW and PAW+LTP can effectively promote wheat seed germination and seedling growth，and LTP+W⁃7.5 treatment is the best.

Key words: Wheat, Seed germination, Seedling growth, Low?temperature plasma, Plasma?activated water, Vigor index, Wetting property, Reactive oxygen and nitrogen species

摘要： 以济麦22、百农307为材料，研究等离子体活化水（PAW，制备时间分别为5.0、7.5、10.0 min，分别命名为PAW-5、PAW-7.5、PAW-10）及其与低温等离子体（LTP）联合处理（LTP+W-5、LTP+W-7.5、LTP+W-10）对小麦种子萌发及幼苗生长的影响，以期为PAW、LTP在小麦种子活力提高方面的应用提供理论依据。结果表明，2个小麦品种种子均于LTP处理9 s时的发芽率及发芽指数达到最佳，因此选用LTP处理时间9 s进行PAW与LTP联合作用效果研究。PAW、PAW与LTP联合处理均能提高小麦种子的发芽率、株高、幼苗鲜质量和干质量，并显著提高种子的活力指数，总体以LTP+W-7.5处理效果最好；未经LTP处理的种子与蒸馏水之间的接触角最大，表明其润湿性能最弱，相较之下，未经LTP处理的种子与PAW之间的接触角有所下降，而经LTP处理后的种子与蒸馏水或PAW之间的接触角极显著下降；与蒸馏水处理相比，PAW及其与LTP联合处理的小麦幼苗中的活性氧氮（RONS）含量总体上显著增加，而仅LTP处理的小麦幼苗中RONS含量无显著变化。综上，PAW及其与LTP联合处理均有效地促进了小麦种子萌发及幼苗生长，其中LTP+W-7.5处理的促进效果最佳。

关键词: 小麦, 种子萌发, 幼苗生长, 低温等离子体, 等离子体活化水, 活力指数, 润湿性能, 活性氧氮