Effect of Biochar Composite Super Absorbent Polymers Combined with Arbuscular Mycorrhizal Fungi on Tobacco Growth and Soil Microenvironment under Drought Stress

doi:10.15933/j.cnki.1004-3268.2026.01.008

Abstract

Abstract: Through pot experiments，the effects of the dosage of biochar composite super absorbent polymers（SAP）（0，2，4，8 g/pot，denoted as S₀，S₁，S₂，S₃）and inoculation with arbuscular mycorrhizal fungi［Claroideoglomus etunicatum（CE），Glomus versiforme（GV），Funneliformes mossae（FM）and non inoculation control（CK）］on the growth，root configuration parameters，and soil microenvironment of Yuyan 5 tobacco under drought stress were investigated.The results showed that under drought stress，the SAP could promote the infection of arbuscular mycorrhizal fungi（AMF） on tobacco roots and had a positive effect on the mycorrhizal effect.The mycorrhizal infection rate was the highest（53.0%）and the number of vesicles was the highest（15.3 vesicles/cm）under the SAP dosage S2 treatment inoculated with FM.Under the same AMF inoculation treatment，with the increase of SAP dosage，tobacco growth indicators and soil physicochemical indicators（except for soil fungi，0.25—0.5mm，and <0.25 mm soil aggregate content）showed a trend of first increasing and then decreasing，and reached their peak under the S2 SAP dosage treatment. Under the treatment of S2 SAP，compared to the treatment without adding SAP or inoculating with AMF，the total length，total projected area，total volume，root tip number，and root vitality of tobacco roots treated with FM increased by 78.7%，130.1%，141.0%，44.7%，and 83.9%，respectively.The number of soil fungi decreased by 73.7%，and the content of bacteria and actinomycetes increased by 134. 8% and 190.7%，respectively.Under drought stress，inoculation with AMF treatment could increase the activities of urease，cellulase，acid protease，acid phosphatase，sucrase，and chitinase in tobacco root soil to varying degrees.Compared to the treatment without adding SAP or inoculating with AMF，the FM treatment under S2 dosage increased the activities of urease，cellulase，acid protease，acid phosphatase，sucrase and chitinase activity in tobacco soil by 120.6%，94.9%，75.0%，200.0%，55.9% and 75.0%，respectively.In addition，inoculation with AMF treatment could increase the total nitrogen，available phosphorus，available potassium，and organic matter content of tobacco rhizosphere soil to varying degrees，and increase the soil aggregate content of tobacco roots（>2 mm and 0.5—2 mm）.There was no significant change in the soil aggregate content of 0.25—0.5 mm，but it reduced the soil aggregate content of <0.25 mm.In summary，biochar composite SAP has a positive effect on plants，which can increase tobacco biomass，promote AMF infection，and enhance the positive effect of AMF on tobacco growth and drought resistance to a certain extent.Among them，the best effect on tobacco drought resistance is achieved when the SAP dosage is 4 g/pot（S₂）combined with FM.

Key words: Tobacco, Super absorbent polymer, Arbuscular mycorrhizal fungi, Root system configuration, Soil enzyme activities

摘要： 通过盆栽试验，探讨生物炭复合保水剂用量（0、2、4、8 g/盆，记为S₀、S₁、S₂、S₃）和接种丛枝菌根真菌［幼套近明球囊霉（Claroideoglomus etunicatum，CE）、变形球囊霉（Glomus versiforme，GV）、摩西斗管囊霉（Funneliformis mosseae，FM）和不接种对照（CK）］对干旱胁迫下豫烟5号烟草生长、根系构型参数以及土壤微环境的影响。结果表明，干旱胁迫下，生物炭复合保水剂能促进丛枝菌根真菌（Arbuscular mycorrhizal fungi，AMF）对烟草根系的侵染，对菌根效应的发挥具有积极作用，二者联合施用具有协同增效作用，保水剂用量S₂处理下接种FM处理的菌根侵染率最高（53.0%），泡囊数最多（15.3个/cm）。同一AMF接种处理下，随保水剂用量的增加，烟草生长指标和土壤理化指标（除土壤真菌和0.25~0.5 mm、<0.25 mm土壤团聚体含量外）整体上表现为先增后降趋势，且在保水剂用量S₂处理下达到峰值。保水剂用量S₂处理下，与S₀CK处理相比，FM处理的烟草根系总长度、总投影面积、总体积、根尖数和根系活力分别增加78.7%、130.1%、141.0%、44.7%和83.9%，土壤真菌数量下降73.7%，细菌和放线菌数量分别增加134.8%和190.7%。干旱胁迫下接种AMF处理整体上能不同程度提高烟草根系土壤脲酶、纤维素酶、酸性蛋白酶、酸性磷酸酶、蔗糖酶和几丁质酶活性，保水剂用量S2处理下，与S0CK处理相比，FM处理的烟草土壤脲酶、纤维素酶、酸性蛋白酶、酸性磷酸酶、蔗糖酶和几丁质酶活性分别增加120.6%、94.9%、75.0%、200.0%、55.9%和75.0%。此外，接种AMF处理能不同程度提高烟草根围土壤全氮、速效磷、速效钾和有机质含量，增加烟草根系>2 mm、0.5~2 mm的土壤团聚体含量，对0.25~0.5 mm土壤团聚体含量影响无明显规律，降低<0.25 mm的土壤团聚体含量。综上，生物炭复合保水剂对植物具有正效应，可增加烟草生物量，促进AMF侵染，并可在一定程度上增强AMF对烟草生长和抗旱性的正效应，其中以保水剂用量为4 g/盆（S₂）并配施FM对烟草抗旱性的效果最好。

关键词: 烟草, 保水剂, 丛枝菌根真菌, 根系构型, 土壤酶活性

CLC Number:

S511

GUO Xiaoqin, LI Qinkui, WU Guangpeng, ZHANG Xiaoquan. Effect of Biochar Composite Super Absorbent Polymers Combined with Arbuscular Mycorrhizal Fungi on Tobacco Growth and Soil Microenvironment under Drought Stress[J]. Journal of Henan Agricultural Sciences, 2026, 55(1): 88-100.

郭晓琴, 李钦奎, 武广鹏, 张小全. 干旱胁迫下生物炭复合保水剂配施丛枝菌根真菌对烟草生长及土壤微环境的影响[J]. 河南农业科学, 2026, 55(1): 88-100.

Figures/Tables 8

References

［1］彭超军，华夏，王松峰，等. 干旱胁迫对不同小麦品种灌浆期旗叶光合及生理特性的影响［J］.河南农业科学，2025，54（2）：40‐47.

PENG C J，HUA X，WANG S F，et al. Effect of drought stress on photosynthetic and physiological characteristics of flag leaves of different wheat varieties at filling stage［J］.Journal of Henan Agricultural Sciences，2025，54（2）：40‐47.

［2］HU Z R，LI Y Y，YANG J S，et al. The positive impact of the NtTAS14‑like1 gene on osmotic stress response in Nicotiana tabacum［J］. Plant Cell Reports，2023，43（1）：25.

［3］HU Z R，HE Z X，LI Y Y，et al. Transcriptomic and metabolic regulatory network characterization of drought responses in tobacco［J］. Frontiers in Plant Science，2023，13：1067076.

［4］王小展，杨春艳，史常青，等. 不同温度和冷冻/冻融循环对保水剂吸持水性能的影响［J］. 农业工程学报，2025，41（4）：59‐69.
WANG X Z，YANG C Y，SHI C Q，et al. Effects of different temperatures and freezing/freezing‐thawing cycles on the water absorption and holding properties of superabsorbent polymers［J］. Transactions of the Chinese Society of Agricultural Engineering，2025，41 （4）：59‐69.

［5］韩雅婷，郭天骄，贾国涛，等. 保水剂和抗蒸腾剂配施对土壤水分和云烟87化学成分、碳氮代谢的影响［J］. 河南农业科学，2023，52（11）：57‐65.
HAN Y T，GUO T J，JIA G T，et al. Effects of combined application of water‐retaining agent and antitranspirant on soil moisture，chemical composition and carbon and nitrogen metabolism of Yunyan 87［J］.Journal of Henan Agricultural Sciences，2023，52（11）：57‐65.

［6］GUO J Z，SHI W J，WEN L J，et al. Effects of a super‐absorbent polymer derived from poly‐γ‐glutamic acid on water infiltration，field water capacity，soil evaporation， and soil water‐stable aggregates［J］.Archives of Agronomy and Soil Science，2020，66（12）：1627‐1638.

［7］马宇，王硕立，赵曦，等. 保水剂对豫西南半湿润区雪茄烟田耕层土壤理化性质及烟株生长发育的影响［J］.河南农业科学，2024，53（2）：46‐55.
MA Y，WANG S L，ZHAO X，et al. Effects of water‐retaining agent on the physical and chemical properties of topsoil and the growth and development of tobacco plants in cigar tobacco field in the semi‐humid area of southwest Henan［J］. Journal of Henan Agricultural Sciences，2024，53（2）：46‐55.

［8］程红胜，沈玉君，孟海波，等. 生物炭基保水剂对土壤水分及油菜生长的影响［J］. 中国农业科技导报，2017，19（2）：86‐92.
CHENG H S，SHEN Y J，MENG H B，et al. Effects of biochar‐based super absorbent on soil moisture and rape growth［J］. Journal of Agricultural Science and Technology，2017，19（2）：86‐92.

［9］夏茂林，刘云飞，张承吉，等. 新型复合保水剂对半干旱区烤烟生长和生理特性的影响［J］. 中国烟草科学，2023，44（1）：32‐37.
XIA M L，LIU Y F，ZHANG C J，et al. Effects of new composite water retention agents on growth and physiological characteristics of flue‐cured tobacco in semi‐arid areas［J］. Chinese Tobacco Science，2023，44（1）：32‐37.

［10］韩金吉，沈小奥，杨帆，等. 丛枝菌根真菌（AMF）介导植物矿质元素吸收机制的研究进展［J］. 草地学报，2023，31（6）：1609‐1621.
HAN J J，SHEN X A，YANG F，et al. Research progress on the mechanism of arbuscular mycorrhizal fungi（AMF）mediated mineral elements uptake by plants［J］. Acta Agrestia Sinica，2023，31（6）：1609‐1621.

［11］TANG B，MAN J，LEHMANN A，et al. Arbuscular mycorrhizal fungi attenuate negative impact of drought on soil functions［J］. Global Change Biology，2024，30（7）：e17409.

［12］WEI D，TIAN B J，WU Q Z，et al. Water stress combining weather condition shapes wheat yield and inter‐annual yield variability：Field observations from a six‐year study［J］. European Journal of Agronomy，2024，159：127291.

［13］张斌，吕玉峰，李利，等. 丛枝菌根真菌接种与磷添加对干旱胁迫燕麦土壤微生物生物量及酶活性的影响［J］. 生态学杂志，2024，43（3）：644‐655.
ZHANG B，LÜ Y F，LI L，et al. Effects of AMF and phosphorus application on microbial biomass and enzyme activities in oat soil under drought stress［J］.Chinese Journal of Ecology，2024，43（3）：644‐655.

［14］宗建伟，邓海芳，蔡沅原，等. AM真菌对干旱胁迫下文冠果根系形态和叶片结构耦合的影响［J］.生物技术通报，2025，41（6）：167‐178.
ZONG J W，DENG H F，CAI Y Y，et al.Coupling effect of AM fungi on the root morphology and leaf structure of Xanthoceras sorbifolium Bunge under drought stress［J］.Biotechnology Bulletin，2025，41（6）：167‐178.

［15］孙文秀，邵晨阳，陈妍妍，等. 干旱胁迫下2种内生真菌对烟草生理生化指标及NAC基因表达的影响［J］. 华北农学报，2024，39（1）：113‐119.
SUN W X，SHAO C Y，CHEN Y Y，et al. Effects of two endophytic fungi on physiological and biochemical indexes and NAC gene expression in tobacco under drought stress［J］. Acta Agriculturae Boreali‐Sinica，2024，39（1）：113‐119.

［16］王思雨，魏涵，陈科宇，等. 丛枝菌根真菌（AMF）孢子、菌丝密度及侵染率定量测定方法［J］. 微生物组实验手册，2022，101：e2104253.
WANG S Y，WEI H，CHEN K Y，et al. Practical methods for arbuscular mycorrhizal fungal spore density，hyphal density and colonization rate of AMF［J］. Microbiome Protocols eBook， 2022， 101：e2104253.

［17］鲍士旦. 土壤农化分析［M］. 北京：中国农业出版社，1998.
BAO S D. Soil agricultural chemistry analysis［M］.Beijing：China Agriculture Press，1998.

［18］关松荫. 土壤酶及其研究法［M］. 北京：农业出版社，1986.
GUAN S Y. The study method of soil enzyme［M］.Beijing：Agriculture Press，1986.

［19］李振高，骆永明，滕应. 土壤与环境微生物研究法［M］. 北京：科学出版社，2008.
LI Z G，LUO Y M，TENG Y. Soil and environmental microbial research method［M］.Beijing：Science Press，2008.

［20］彭珏，陈家赢，王军光，等. 中国典型地带性土壤团聚体稳定性与孔隙特征的定量关系［J］. 农业工程学报，2022，38（18）：113‐121.
PENG J，CHEN J Y，WANG J G，et al. Linking aggregate stability to the characteristics of pore structure in different soil types along a climatic gradient in China［J］. Transactions of the Chinese Society of Agricultural Engineering，2022，38（18）：113‐121.

［21］LI T，ZHOU X N，WANG Y X，et al. AtCIPK20 regulates microtubule stability to mediate stomatal closure under drought stress in Arabidopsis［J］. Plant，Cell & Environment，2024，47（12）：5297‐5314.

［22］LI Y L，WANG X Y，CHEN X Y，et al. Functions of arbuscular mycorrhizal fungi in regulating endangered species Heptacodium miconioides growth and drought stress tolerance［J］.Plant Cell Reports，2023，42（12）：1967‐1986.

［23］王诗琪，杨柳青，廖飞勇，等. 干旱胁迫下基质和保水剂对3种景天属植物生长和生理的影响［J］.中南林业科技大学学报，2025，45（6）：55‐66.
WANG S Q，YANG L Q，LIAO F Y，et al. Effects of substrate and water retaining agent on the growth and physiology of three Sedum plants under drought stress［J］. Journal of Central South University of Forestry &Technology，2025，45（6）：55‐66.

［24］LIANG J，SUN Z Q，QU Z W，et al. Long‐term effect of an ectomycorrhizal inoculum and other treatments on survival and growth of Populus hopeiensis Hu et Chow［J］.Forest Ecology and Management，2010，259（12）：2223‐2232.

［25］于萌，张永帅，付伟，等. 保水剂和丛枝菌根真菌异形根孢囊霉对紫花苜蓿生长与抗旱性的影响［J］.菌物学报，2019，38（11）：1976‐1991.
YU M，ZHANG Y S，FU W，et al. Effects of arbuscular mycorrhizal fungi Rhizophagus irregularis and super absorbent polymers on growth and drought tolerance of Medicago sativa［J］. Mycosystema，2019，38（11）：1976‐1991.

［26］MANSOOR S，CHUNG Y S. Functional phenotyping：Understanding the dynamic response of plants to drought stress［J］. Current Plant Biology，2024，38：100331.

［27］于明华，郑婷，马烈，等. 干旱胁迫下丛枝菌根真菌对月季生理指标的影响［J］.分子植物育种，2024，22（16）：5415‐5423.
YU M H，ZHENG T，MA L，et al. Effects of arbuscular mycorrhizal fungi on physiological index of rose cultivars under drought stress［J］. Molecular Plant Breeding，2024，22（16）：5415‐5423.

［28］梁圣敏，张菲，吴强盛. 丛枝菌根真菌通过调节枳根系多胺提高抗旱性［J］. 园艺学报，2023，50（12）：2680‐2688.
LIANG S M，ZHANG F，WU Q S. Arbuscular mycorrhizal fungi improve drought tolerance of trifoliate orange seedlings by regulating root polyamines ［J］. Acta Horticulturae Sinica，2023，50（12）：2680‐2688.

［29］WANG Z H，ZHANG S Q，LIANG J W，et al. Rhizophagus irregularis regulates RiCPSI and RiCARI expression to influence plant drought tolerance［J］.Plant Physiology，2024，197（1）：kiae645.

［30］王紫瑄，解甜甜，王雅茹，等. 丛枝菌根真菌（AMF）对蒙古沙冬青幼苗的促生特性及作用机制［J］.干旱区研究，2023，40（1）：78‐89.
WANG Z X，XIE T T，WANG Y R，et al. Growth promotion and mechanism of arbuscular mycorrhizal fungi（AMF）on Ammopiptanthus mongolicus seedlings［J］. Arid Zone Research，2023，40（1）：78‐89.

［31］ZHANG W，XIA K L，FENG Z W，et al. Tomato plant growth promotion and drought tolerance conferred by three arbuscular mycorrhizal fungi is mediated by lipid metabolism［J］. Plant Physiology and Biochemistry，2024，208：108478.

［32］高齐，盛阳阳，赵志伟，等. 不同类型保水剂对旱作谷子水分利用的影响［J］.干旱区资源与环境，2023，37（9）：154‐163.
GAO Q，SHENG Y Y，ZHAO Z W，et al. Effects of different types of water retaining agents on water use of rainfed millet［J］. Journal of Arid Land Resources and Environment，2023，37（9）：154‐163.

［33］李华，陈亮，杜雷超，等. 保水剂施用深度和施用量对玉米光合特性、产量和水分利用效率的影响［J］.河南农业科学，2025，54（5）：10‐22.
LI H，CHEN L，DU L C，et al. Effects of different application depth and application amount of water retaining agent on photosynthetic characteristics，yield and water use efficiency of maize［J］. Journal of Henan Agricultural Sciences，2025，54（5）：10‐22.

［34］郭维，肖志鹏，向鹏华，等. 保水剂施用对植烟紫色土的改良效应及烤烟品质的影响［J］. 中国烟草科学，2023，44（1）：38‐43.
GUO W，XIAO Z P，XIANG P H，et al. Effects of a water‐retaining agent on tobacco field and the quality of flue‐cured tobacco leaves in a purple soil［J］.Chinese Tobacco Science，2023，44（1）：38‐43.

［35］张玉斌，景泉，崔金虎，等. 富钼专用保水剂对东北典型土壤保墒性能及大豆幼苗生长的影响［J］. 中国水土保持科学（中英文），2024，22（1）：63‐69.
ZHANG Y B，JING Q，CUI J H，et al. Effects of molybdenum‐rich super absorbent polymers on soil water retention and soybean seedling growth in typical soils of NorthEast China［J］. Science of Soil and Water
Conservation，2024，22（1）：63‐69.

［36］刘迎超，于涛，孙光伟，等. 保水剂施用对恩施烟区上部烟叶生长发育的影响［J］.中国烟草科学，2020，41（3）：53‐57.
LIU Y C，YU T，SUN G W，et al. Effects of super absorbent polymer on growth and development of upper tobacco leaves in the Enshi tobacco growing area［J］. Chinese Tobacco Science，2020，41（3）：53‐57.

［37］PENG Y M，XU H S，SHI J，et al. Soil microbial composition， diversity， and network stability in intercropping versus monoculture responded differently to drought［J］. Agriculture，Ecosystems & Environment，2024，365：108915.

［38］BI Y L，ZHANG K，XIAO L，et al.The legacy effects of mycorrhizal fungal inoculation alleviate soil nitrogen limitations by modulating the homeostasis of extracellular enzymes and fungal function in arid mining regions［J］.Soil Use and Management，2024，40 （4）：e13130.

［39］许庆龙，刘晓敏，徐小兵，等. 4种丛枝菌根真菌对南高丛蓝莓抗旱性的影响［J］.浙江大学学报（农业与生命科学版），2016，42（4）：427‐434.
XU Q L，LIU X M，XU X B，et al. Effects of four arbuscular mycorrhizal fungi on tolerance of Vaccinium corymbosum to drought stress［J］.Journal of Zhejiang University（Agriculture and Life Sciences），2016，42（4）：427‐434.

［40］张宇君，王普昶，尚以顺，等. 保水剂对盘江白刺花幼苗根系生长及根际土壤酶活性的影响［J］. 北方园艺，2020（16）：92‐97.
ZHANG Y J，WANG P C，SHANG Y S，et al.Effects of super absorbent polymers on root growth and enzyme activity in rhizosphere soil of Sophora davidii seedlings in Panjiang［J］. Northern Horticulture，2020 （16）：92‐97.

［41］毕银丽，张延旭，江彬，等. 水分胁迫下AM真菌与解磷细菌协同对玉米生长及土壤肥力的影响［J］. 煤炭学报，2019，44（12）：3655‐3661.
BI Y L，ZHANG Y X，JIANG B，et al. Effects of AM fungi and phosphate‐solubilizing bacteria inoculation on maize growth and soil fertility under water stress［J］.Journal of China Coal Society，2019，44（12）：3655‐3661.

［42］李越. 接种AM真菌对干旱胁迫燕麦土壤和植株氮、磷相关指标的影响［D］. 太谷：山西农业大学，2022.
LI Y. Effects of AM fungi inoculation on nitrogen and phosphorus related indexes of oat soil and plant under drought stress［D］. Taigu： Shanxi Agricultural University，2022.

［43］聂晖，李翀，张金池，等. 不同溶岩微生物菌剂与保水剂配比对土壤养分和酶活性的影响［J］. 水土保持通报，2022，42（4）：107‐115.
NIE H，LI C，ZHANG J C，et al. Effects of different ratios of mineral‐solubilizing microbes and water‐retaining agents on soil nutrients and enzyme activities［J］. Bulletin of Soil and Water Conservation，2022，42（4）：107‐115.

［44］严定伟，王海洋，王俊，等. 新型复合保水剂对植烟土壤性质及烤烟有机酸含量的影响［J］.山东农业科学，2024，56（11）：100‐105.
YAN D W，WANG H Y，WANG J，et al. Effects of new compound water‐retaining agent on tobacco‐growing soil properties and organic acid content of flue‐cured tobacco［J］. Shandong Agricultural Sciences，2024，56（11）：100‐105.

［45］王纪坤，安锋，周立军，等. 保水剂施用量对胶园土壤微生物和土壤酶活性及产量的影响［J］.西南农业学报，2023，36（7）：1424‐1431.
WANG J K，AN F，ZHOU L J，et al. Effects of super absorbent polymers dosages on soil microorganism，soil enzyme activities and yield in rubber plantations［J］.Southwest China Journal of Agricultural Sciences，2023，36（7）：1424‐1431.

［46］李希，贺纪正，郑袁明，等. 新型保水剂应用于土壤-小白菜系统的环境安全评价［J］. 环境科学，2014，35（2）：780‐785.
LI X，HE J Z，ZHENG Y M，et al. Environmental safety assessment on the new super absorbent polymers applied into a soil‐Chinese cabbage system［J］.Environmental Science，2014，35（2）：780‐785.

[1]	YANG Shaoxin, XU Delong, DAI Youzong, ZHENG Fang, WANG Shengxiao, YE Changwen, HE Yuan, YANG Liqiang, HAO Qidong, XU Meng, HUANG Kuo, HU Yanqi. Effect of Fermentation on Culturable Microbial Dominant Flora and Quality of Cigar Filler Tobacco Leaves [J]. Journal of Henan Agricultural Sciences, 2026, 55(1): 162-172.
[2]	LI Zhaoyang, WANG Ruigang, JIA Zhenyu, ZHANG Yuning, LI Hongchen, XUE Songge, CHEN Haiyang, LI Lihua, ZHANG Xiaoquan. Functional Analysis of NtWRKY65 Gene in Tobacco under Low Nitrogen Stress [J]. Journal of Henan Agricultural Sciences, 2025, 54(9): 53-60.
[3]	LIU Mengfen, MAI Xuan, LIU Mengmeng, XUE Fang, LI Dong, YE Changwen, ZHU Beibei, XU Pengfei, CHEN Guokang, HUANG Kuo. Pathogens Identification and Study on Regularity of Mildew in Cigar Tobacco Leaves from Sichuan [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 111-121.
[4]	ZHANG Changcheng, JIANG Jun, PAN Shouhui, XUE Yuan, ZHANG Quan, LI Zikuo, WANG Hancheng, SHI Caihua, FANG Shouguo, ZHANG Songbai, LI Xiquan. Molecular Identification and Complete Sequence Analysis of Chilli Veinal Mottle Virus GZ‑tobacco Isolate [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 102-110.
[5]	JIN Jianghua, XIA Bing, LI Xu, YU Dapeng, GUO Wei, CHEN Tao, WU Wenxin, LI Sijun, WANG Xinyue, HUANG Jie, DENG Xiaohua. Fuzzy Comprehensive Evaluation of Leaf Quality and Economic Traits of Paddy‑Stubble Tobacco with Root Zone Application of Microbial Fertilizer [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 82-91.
[6]	QIAN Jiancai, ZHU Yufei, DAN Dongchun, HUANG Wuxing, ZHANG Li. Effects of Different Ridging Time on Growth Period and Quality of Flue‑cured Tobacco in Luoyang Tobacco Growing Area [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 60-68.
[7]	WU Jinlu, HU Anfu, JIANG Jian, XIA Qian, WANG Jun, XIAO Weiqiang, ZHOU Guojun, WU Jian, XU Jian, FANG Mengxiang. Research Progress on Generation，Migration and Release of Moisture in Heated Tobacco Product [J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 15-25.
[8]	ZHAI Shunlong, ZHAO Yongwei, CHANG Jianbo, WANG Jian’an, HE Fan, LIU Lingling. Effects of Heat Preservation Treatment on Nitrogen Metabolism during Tobacco Curing in Bulk Curing Barn [J]. Journal of Henan Agricultural Sciences, 2025, 54(6): 175-184.
[9]	ZOU Quan, XING Weiming, WANG Ruoding, ZHOU Wenjun. Effects of Root Application of AMF and SL on Chlorophyll Fluorescence and Antioxidant System of Lolium perenne under Saline Alkali Stress [J]. Journal of Henan Agricultural Sciences, 2025, 54(6): 72-83.
[10]	LI Xipeng, KONG Jinling, HONG Hu, FU Yunpeng1, YAO Pengwei, FU Quanshan, HOU Zhenwu, FU Bo, LIU Youjie, WANG Jing. Effects of Different Adjuvants on Weed Control Efficiency of Herbicides，Growth and Economic Traits of Tobacco [J]. Journal of Henan Agricultural Sciences, 2025, 54(5): 78-86.
[11]	WANG Tingting, ZHAO Yuanyuan, XIA Yulan, WANG Dexun, LI Juan, SUN Junwei, SHI Hongzhi. Effects of Exogenous Spraying of Methyl Jasmonate on Maturation and Cured Quality of Late‐maturing Upper Tobacco Leaves [J]. Journal of Henan Agricultural Sciences, 2025, 54(5): 49-58.
[12]	GE Menglong, HE Bing, BAI Feng, HUANG Peiyuan, HE Mengying, LI Jianhua, LI Yuyang, SHEN Hongtao, GAO Jiakai, WANG Yanfang, LIU Ling. Effects of Flue‐cured Tobacco and Wheat Intercropping on Soil Microorganisms and Fertility in Xuchang Tobacco Growing Area [J]. Journal of Henan Agricultural Sciences, 2025, 54(4): 66-79.
[13]	WANG Mingxin, LIU Lei, DENG Zhaolong, WANG Haoyang, LIU Pengfei, JIN Weihuan, LI Junying, GUO Hongxiang. Characterisation of the NtHPL Genes and Their Expression Pattern under TMV Stress in Tobacco [J]. Journal of Henan Agricultural Sciences, 2025, 54(4): 47-56.
[14]	LUO Haitao, WANG Shuoying, QI Yating, LI Ruili, CHEN Dong, RAO Chaoqi, ZHANG Junsong, ZOU Enkai. Effect of Different Enzymes on the Quality Improvement Effect of Discarded Tobacco Extract [J]. Journal of Henan Agricultural Sciences, 2025, 54(3): 170-180.
[15]	LIANG Miao, LIU Yuxuan, ZHU Haibo, SU Man, ZHENG Yongjie, XIE Yifei, JIANG Xi, ZHANG Junsong, ZHU Yuanyang. Analysis of Thermogravimetric Characteristics and Stepwise Thermal Release Aroma Components of Different Types of Tobacco [J]. Journal of Henan Agricultural Sciences, 2025, 54(3): 157-169.