Characterisation of the NtHPL Genes and Their Expression Pattern under TMV Stress in Tobacco

doi:10.15933/j.cnki.1004-3268.2025.04.005

Abstract

Abstract: Hydroperoxide lyase（HPL）is a key enzyme in the catalytic fatty acid oxidation pathway and belongs to the cytochrome P450（CytP450）superfamily. HPL plays an essential role in plant responses to both biotic and abiotic stresses，contributing significantly to the regulation of stress tolerance.In this study，the NtHPL gene family in tobacco was identified using genomic data and comprehensively analyzed for their physicochemical properties，protein structures，conserved motifs，and response patterns to tobacco mosaic virus（TMV）.The results indicated that nine NtHPL family members were identified，distributed across eight chromosomes，and classified into three subclasses：B，C，and D.The amino acid lengths of these members ranged from 437 to 527 residues，with theoretical isoelectric points（pI）varying between 5.84 and 9.15.Subcellular localization predictions suggested that these proteins were primarily localized in chloroplasts and cytoplasm.Expression analysis revealed that upon TMV infection，five NtHPL genes were upregulated，one was downregulated，and three exhibited no significant differential expression in tobacco leaves. Overexpression of NtHPL1 enhanced tobacco resistance to TMV，as demonstrated by experimental evidence.These findings collectively suggest that NtHPL may be involved in the regulatory mechanisms underlying tobacco defense against TMV.

Key words: Tobacco, Hydroperoxide lyase, TMV, Cytochrome P450

摘要： 脂氢过氧化物裂解酶（Hydroperoxide lyase，HPL）为细胞色素P450（CytP450）蛋白质家族成员，是催化脂肪酸氧化途径中的一个关键酶。HPL参与植物的生物和非生物胁迫，在增强植物抵御外部胁迫调控中发挥重要作用。通过对基因组数据分析，鉴定烟草中NtHPL基因家族成员并分析其理化性质、蛋白质结构、保守基序及其对烟草花叶病毒（Tobacco mosaic virus，TMV）的响应特性。结果表明，共鉴定出NtHPL家族成员9个，分布于8条染色体，分属于B、C、D组。NtHPL基因家族成员的氨基酸数量为437~527个，理论等电点为5.84~9.15，预测定位在叶绿体和细胞质中。通过对NtHPL基因表达量分析发现，TMV侵染后，叶片中共有5个NtHPL基因上调，1个NtHPL基因下调，3个基因表达量没有差异。过表达NtHPL1发现，NtHPL1能提高烟草对TMV的抗性。根据以上结果，NtHPL可能参与烟草对TMV的抗性调控。

关键词: 烟草, 脂氢过氧化物裂解酶, 烟草花叶病毒, 细胞色素P450

CLC Number:

S572

WANG Mingxin, LIU Lei, DENG Zhaolong, WANG Haoyang, LIU Pengfei, JIN Weihuan, LI Junying, GUO Hongxiang. Characterisation of the NtHPL Genes and Their Expression Pattern under TMV Stress in Tobacco[J]. Journal of Henan Agricultural Sciences, 2025, 54(4): 47-56.

王明鑫, 刘磊, 邓兆龙, 王昊阳, 刘鹏飞, 金维环, 李俊营, 郭红祥. 烟草NtHPL基因特征及其在TMV胁迫下的表达分析[J]. 河南农业科学, 2025, 54(4): 47-56.

Figures/Tables 9

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