Study on Antifungal Activity and Nano‑selenization Application of Paenilbacillus polymyxa

doi:10.15933/j.cnki.1004-3268.2025.01.010

Abstract

Abstract: In order to screen the microorganisms with good control effect for the control of crop fungal diseases，the disease resistance and nano‑selenization of Paenobacillus polymyxa KZJ‑1 were studied.The results showed that KZJ‑1 bacteriological solution had good inhibition effect on Colletotrichum micotianaeThanatephorus cucumeris，Thielaviopsis basicola and Phytophthora parasitica，with inhibition rates of 49.78%，53.64%，46.42% and 58.85%，respectively.In addition，the strain KZJ‑1 could biosynthesize nano‑selenium，and the active solution of nano‑selenium with different concentrations had a good inhibitory effect on Phytophthora parasitica，and the inhibitory effect was enhanced with the increase of the concentration of sodium selenite added.At the same time，the pot experiment results showed that KZJ‑1 and its nano‑selenium active bacterial solution could effectively prevent and control tobacco black shank disease and promote the growth of tobacco plants.The disease index of tobacco black shank disease treated with nano‑selenium active bacterial solution was the lowest after 7 days of irrigation，and the control effect reached 90.23%，and the control effect decreased slightly to 83.32% after 14 days，both of which were significantly higher than that of the control.The growth rate of plant height，maximum leaf length and maximum leaf width were 16.21%，31.74% and 33.61%，respectively.It can be seen that the biosynthesis of nano‑selenium by strain KZJ‑1 enhanced the disease resistance and growth promotion ability of the original strain，and had certain practical application value in production.

Key words: Paenobacillus polymyxa, Tobacco disease, Fungi, Disease control, Nano?selenium, Growth promotion

摘要： 为筛选防效优良的微生物应用于作物真菌类病害防治，对前期筛选得到的多黏类芽孢杆菌KZJ-1进行了抗病及纳米硒化研究。结果显示，KZJ-1菌液对烟草炭疽病菌、靶斑病菌、根腐病菌、黑胫病菌均具有较好的抑制效果，抑制率分别为49.78%、53.64%、46.42%、58.85%。菌株KZJ-1可以生物合成纳米硒，不同浓度的纳米硒活性菌液对黑胫病菌均具有较好的抑制效果，随着加入的亚硒酸钠浓度增大，抑制效果增强。同时，盆栽试验结果显示，KZJ-1菌液及其纳米硒活性菌液均可有效防治烟草黑胫病并促进烟株生长，灌施纳米硒活性菌液7 d后烟草黑胫病病情指数最低，防效达90.23%，14 d后防效稍有下降，仍高达83.32%，均显著高于药剂对照；纳米硒活性菌液灌根的本氏烟生长发育最好，株高、最大叶长、最大叶宽的增长率分别为16.21%、31.74%、33.61%。可见，菌株KZJ-1生物合成纳米硒增强了原始菌株的抗病及促生能力，在生产上具有一定的实际应用价值。

关键词: 多黏类芽孢杆菌, 烟草病害, 真菌, 病害防治, 纳米硒, 促生

CLC Number:

S476
S435.72

YUAN Lianlian, LIU Tianbo, LI Xiaodong, DENG Zhengyu, TENG Kai, SHEN Lili, JIAO Yubing, WANG Yingwen, YANG Jinguang, LI Ying. Study on Antifungal Activity and Nano‑selenization Application of Paenilbacillus polymyxa[J]. Journal of Henan Agricultural Sciences, 2025, 54(1): 101-108.

袁莲莲, 刘天波, 李晓东, 邓征宇, 滕凯, 申莉莉, 焦裕冰, 王英文, 杨金广, 李莹. 多黏类芽孢杆菌抗真菌活性及其纳米硒化应用[J]. 河南农业科学, 2025, 54(1): 101-108.

Figures/Tables 10

References

［1］蔺忙碌.植物病害防治中的生物防治技术研究［J］.农业灾害研究，2023，13（10）：13‑15.

LIN M L.Research on biological control technology in plant disease control［J］.Journal of Agricultural Catastrophology，2023，13（10）：13‑15.

［2］李雨欣，戴欣宇，曹雪梅，等.生防菌在植物病害领域的研究进展［J］.湖南生态科学学报，2023，10（2）：109‑116.
LI Y X，DAI X Y，CAO X M，et al. Research progress of microbial antagonists in the field of plant diseases［J］.Journal of Hunan Ecological Science，2023，10（2）：109‑116.

［3］EGAMBERDIYEVA D. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils［J］. Applied Soil Ecology，2007，36（2/3）：184‑189.

［4］RYU C M，KIM J，CHOI O，et al. Improvement of biological control capacity of Paenibacillus polymyxa E681 by seed pelleting on sesame［J］.Biological Control，2006，39（3）：282‑289.

［5］杨少波，刘训理.多粘类芽孢杆菌及其产生的生物活性物质研究进展［J］.微生物学通报，2008，35（10）：1621‑1625.
YANG S B， LIU X L.Research advances in Paenibacillus polymyxa and their bioactive substances［J］.Microbiology，2008，35（10）：1621‑1625.

［6］ZENG H W，COMBS G F. Selenium as an anticancer nutrient：Roles in cell proliferation and tumor cell invasion［J］.The Journal of Nutritional Biochemistry，2008，19（1）：1‑7.

［7］BECK M A，LEVANDER O A，HANDY J. Selenium deficiency and viral infection［J］.The Journal of Nutrition，2003，133（5）：1463S‑1467S.

［8］郑世学，粟静，王瑞，等. 硒是双刃剑？——谈微生物中的硒代谢［J］.华中农业大学学报，2013，32（5）：1‑8.
ZHENG S X，SU J，WANG R，et al.Metabolism of selenium in microorganisms［J］.Journal of Huazhong Agricultural University，2013，32（5）：1‑8.

［9］王文艺，管青松，储茵，等.青阳县土壤硒空间分布特征及富硒区土壤肥力质量评价［J］.山西农业科学，2023，51（9）：1069‑1077.
WANG W Y，GUAN Q S，CHU Y，et al.Spatial distribution characteristics of soil selenium in Qingyang County and soil fertility quality evaluation of selenium‑rich areas［J］.Journal of Shanxi Agricultural Sciences，2023，51（9）：1069‑1077.

［10］张亚峰，马强，姚振，等. 西宁盆地天然富硒作物的筛选与评价［J］.山西农业科学，2023，51（7）：764‑770.
ZHANG Y F，MA Q，YAO Z，et al.Screening and evaluation of natural selenium‑rich crops in the Xining basin［J］.Journal of Shanxi Agricultural Sciences，2023，51（7）：764‑770.

［11］郭肖兰. 硒元素的生物强化途径研究进展［J］.山西农业科学，2023，51（3）：340‑346.
GUO X L.Research progress on biofortified pathways of selenium［J］.Journal of Shanxi Agricultural Sciences，2023，51（3）：340‑346.

［12］余婷婷，袁莲莲，李莹，等. 纳米硒化荧光假单胞菌KBD‑1菌株对烟草病毒病的抑制作用［J］.中国烟草科学，2021，42（5）：43‑49.
YU T T，YUAN L L，LI Y，et al. Inhibition of tobacco virus diseases by nano‑selenated Pseudomonas fluorescens KBD‑1［J］. Chinese Tobacco Science，2021，42（5）：43‑49.

［13］杨辉，王丽红，孔阳，等.微生物合成纳米硒的研究进展［J］.食品工业科技，2020，41（15）：339‑344.

YANG H，WANG L H，KONG Y，et al. Research progress in the synthesis of nano‑selenium by microorganism［J］.Science and Technology of Food Industry，2020，41（15）：339‑344.

［14］BORAH S N，GOSWAMI L，SEN S，et al.Selenite bioreduction and biosynthesis of selenium nanoparticles by Bacillus paramycoides SP3 isolated from coal mine overburden leachate［J］.Environmental Pollution，2021，285：117519.

［15］ABID S，KALIRAJ L，RAHIMI S，et al.Synthesis and characterization of glycol chitosan coated selenium nanoparticles acts synergistically to alleviate oxidative stress and increase ginsenoside content in Panax ginseng［J］.Carbohydrate Polymers， 2021， 267：118195.

［16］GEOFFRION L D，HESABIZADEH T，MEDINA‑CRUZ D，et al.Naked selenium nanoparticles for antibacterial and anticancer treatments［J］.ACS Omega，2020，5（6）：2660‑2669.

［17］黄轶驰，田红旗. 纳米硒制备方法、抗氧化作用机制及临床应用研究进展［J］.山东医药，2021，61（6）：103‑108.
HUANG Y C，TIAN H Q. Research progress on preparation methods， antioxidant mechanism and clinical application of nano‑selenium［J］.Shandong Medical Journal，2021，61（6）：103‑108.

［18］徐境懋，顾明华，韦燕燕，等. 纳米硒和亚硒酸盐对镉污染土壤中水稻镉积累的影响［J］.南方农业学报，2021，52（10）：2727‑2734.
XU J M，GU M H，WEI Y Y，et al.Effects of nano‑selenium and sodium selenite application on cadmium accumulation in rice planting in cadmium contaminated soil［J］.Journal of Southern Agriculture，2021，52（10）：2727‑2734.

［19］翟夜雨，黄五星，袁岐山，等. 植物镉毒害与硒对镉胁迫的缓解作用研究进展［J］.河南农业大学学报，2023，57（3）：372‑382.
ZHAI Y Y，HUANG W X，YUAN Q S，et al.Research progress on plant cadmium toxicity and the alleviation effect of selenium on cadmium stress［J］.Journal of Henan Agricultural University，2023，57（3）：372‑382.

［20］陈大欢.多粘类芽孢杆菌抗真菌活性及对水果采后病害的控制效果研究［D］.石家庄：河北科技大学，2015.
CHEN D H.Study on antifungal activity of Paecilomyces polymyxa and its control effect on postharvest diseases of fruits［D］.Shijiazhuang：Hebei University of Science and Technology，2015.

［21］王钰婷.纳米硒合成菌株的筛选及产硒机理和应用研究［D］.合肥：中国科学技术大学，2019.
WANG Y T. Screening of nano‑Se synthesizing strain and study on selenium production mechanism and application［D］.Hefei：University of Science and Technology of China，2019.

［22］王艺茹，潘培培，沈虎生，等.番茄疫霉根腐病菌拮抗细菌HP8-1的鉴定及生物防治潜力［J］.河南农业大学学报，2024，58（1）：78‑86.
WANG Y R，PAN P P，SHEN H S，et al.Identification and biocontrol potential of antagonist bacteria HP8‑1 against Phytophthora root rot of tomato［J］.Journal of Henan Agricultural University，2024，58（1）：78‑86.
［23］黄艺烁，谢学文，石延霞，等.多粘类芽胞杆菌ZF197对白菜茎基腐病防治效果［J］.园艺学报，2020，47（6）：1059‑1071.
HUANG Y S，XIE X W，SHI Y X，et al.Biocontrol effect of Paenibacillus polymyxa strain ZF197 against base stem rot of Chinese cabbage［J］.Acta Horticulturae Sinica，2020，47（6）：1059‑1071.

［24］陈雪丽，王光华，金剑，等.多粘类芽孢杆菌BRF-1和枯草芽孢杆菌BRF-2对黄瓜和番茄枯萎病的防治效果［J］.中国生态农业学报，2008，16（2）：446‑450.
CHEN X L，WANG G H，JIN J，et al.Biocontrol effect of Paenibacillus polymyxa BRF‑1 and Bacillus subtilis BRF‑2 on Fusarium wilt disease of cucumber and tomato［J］.Chinese Journal of Eco‑Agriculture，2008，16（2）：446‑450.
［25］TAHERI E，TARIGHI S，TAHERI P. Characterization of root endophytic Paenibacillus polymyxa isolates with biocontrol activity against Xanthomonas translucens and Fusarium graminearum［J］.Biological Control，2022，174：105031.
［26］XU S J，KIM B S. Evaluation of Paenibacillus polymyxa strain SC09‑21 for biocontrol of Phytophthora blight and growth stimulation in pepper plants［J］.Tropical Plant Pathology，2016，41（3）：162‑168.
［27］DINH Q T，CUI Z W，HUANG J，et al.Selenium distribution in the Chinese environment and its relationship with human health：A review［J］.Environment International，2018，112：294‑309.
［28］梁郅哲，司振兴，牛慧伟，等.硒对植物的毒害作用研究进展［J］.河南农业科学，2022，51（6）：13‑21.
LIANG Z Z，SI Z X，NIU H W，et al.Research progress on toxic effect of selenium on plants［J］.Journal of Henan Agricultural Sciences，2022，51（6）：13‑21.
［29］ZHANG J S，GAO X Y，ZHANG L D，et al.Biological effects of a nano red elemental selenium［J］.BioFactors，2001，15（1）：27‑38.
［30］李利军，马英辉，卢美欢.纳米硒合成细菌Lxz-41的鉴定、培养条件优化及其在富硒猕猴桃栽培中的应用［J］.食品工业科技，2019，40（21）：110‑117.
LI L J，MA Y H，LU M H. Identification，culture conditions optimization of nano‑selenium synthetic bacteria lxz‑41 and its application in Se‑enriched kiwifruit cultivation［J］.Science and Technology of Food Industry，2019，40（21）：110‑117.
［31］JOSHI S M， DE BRITTO S， JOGAIAH S.Myco‑engineered selenium nanoparticles elicit resistance against tomato late blight disease by regulating differential expression of cellular，biochemical and defense responsive genes［J］.Journal of Biotechnology，2021，325：196‑206.
［32］IKRAM M，RAJA N I，JAVED B，et al. Foliar applications of bio‑fabricated selenium nanoparticles to improve the growth of wheat plants under drought stress［J］.Green Processing and Synthesis，2020，9（1）：706‑714.

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