Abstract: Based on Lonicera japonica genome data，bioinformatics was utilized to identify members of the EIN3/EIL gene family in Lonicera japonica，and systematic analyses were conducted on their physicochemical properties，gene structure，gene expression patterns，and stress response.The results indicated that five LjEIL family members were identified at the whole‐genome level in Lonicera japonica，encoding 592 to 682 amino acids，all of which were acidic hydrophilic nuclear proteins. Chromosomal localization revealed that the LjEIL genes were distributed across four chromosomes，and phylogenetic analysis classified them into two subfamilies：Class Ⅰand Class Ⅲ.Gene structure analysis showed that all LjEIL genes contained the typical EIN3 domain，and their exon‐intron composition was relatively simple.61 cis‐acting elements were predicted in the promoter regions of the LjEIL genes，primarily light‐responsive elements and elements such as methyl jasmonate，gibberellin，anaerobic induction，and low‐temperature response were rich. Transcriptome data analysis revealed that the LjEIL genes exhibited typical tissue‐ and developmental stage‐specific expression patterns，with LjEIL3 showing the highest expression levels in mature leaves，shoot tips，and young bud stage. qRT‐PCR results indicated that drought stress could induce the expression of LjEIL genes，with LjEIL3 experiencing the greatest upregulation，and its expression level continued to increase as drought stress duration extended.Furthermore，Arabidopsis plants transformed with the LjEIL3 gene exhibited a significantly higher survival rate after 10 days of simulated drought treatment with 20% polyethylene glycol 6000 compared to the wild type，further confirming the positive regulatory role of LjEIL3 gene in drought stress.In summary，the LjEIL3 gene plays a functional role in regulating plant responses to drought stress，providing important genetic resources for stress‐tolerant molecular breeding in Lonicera japonica.

Key words: Lonicera japonica, EIN3/EIL gene family, Expression pattern, Drought stress

摘要： 基于金银花基因组数据，利用生物信息学对金银花EIN3/EIL基因家族成员进行了鉴定，并对其理化性质、基因结构、基因表达模式、逆境胁迫响应等进行了系统分析。结果表明，在金银花全基因组水平鉴定到5个LjEIL家族成员，编码592~682个氨基酸，均为酸性亲水核蛋白。染色体定位结果显示，LjEIL基因分布于4条染色体，通过系统发育分析将其划分为Class Ⅰ和Class Ⅲ 2个亚族。基因结构分析结果表明，LjEIL基因均含有典型的EIN3结构域，且外显子-内含子组成较为简单。LjEIL基因启动子区域预测出61个顺式作用元件，以光响应元件为主，并富含茉莉酸甲酯、赤霉素、厌氧诱导、低温响应等元件。转录组数据分析结果显示，LjEIL基因具有典型的组织与发育阶段特异性表达模式，其中LjEIL3在成熟叶、茎尖及幼芽期表达量最高。qRT-PCR结果表明，干旱胁迫均能诱导LjEIL基因表达，其中LjEIL3上调幅度最大，且随干旱胁迫时间的延长表达量持续升高。此外，转LjEIL3 基因拟南芥在20%聚乙二醇6000模拟干旱处理10 d后存活率显著高于野生型，进一步证实了LjEIL3基因在干旱胁迫中发挥正向调控的作用。综上，LjEIL3基因具有调控植物响应干旱胁迫的功能，可为金银花抗逆分子育种提供重要的基因资源。

关键词: 金银花, EIN3/EIL基因家族, 表达模式, 干旱胁迫