Abstract: To provide microbial resources for the research and development of functional microbial fertilizers and the improvement of saline‐alkaline soil，saline‐alkaline tolerant plant growth‐promoting rhizobacteria were isolated from the rhizosphere soil of Phragmites australis in the saline‐alkaline soil of Bachu County，Xinjiang Uygur Autonomous Region.Saline‐tolerant and alkaline‐tolerant microorganisms were enriched and cultivated using high‐saline and high‐alkali LB medium. Their taxonomic status was determined through morphological observation and 16S rRNA gene sequencing.The saline and alkaline tolerance and alkali reduction ability of the strains were measured using LB medium with different NaCl contents and pH values.The plant growth‐promoting related characteristics of the strains，including nitrogen fixation， organic phosphorus solubilization， inorganic phosphorus solubilization，1‐aminocyclopropane‐1‐carboxylate（ACC）deaminase production，siderophore production，and indole‐3‐acetic acid（IAA）production，were detected using different functional identification mediums and the Salkowski colorimetric method.The growth‐promoting ability of the strains were evaluated through plate and pot experiments. The results indicated that one strain of Aidingimonas was screened out and named Aidingimonas sp.Bachu42，with a patent deposit number of CGMCC No.26952.The strain Bachu42 could grow under the conditions of NaCl content ranging from 20 to 200 g/L and pH value ranging from 7.0 to 11.0.This strain had the functions of nitrogen fixation，organic phosphorus solubilization，inorganic phosphorus solubilization，and could produce siderophores，ACC deaminase and IAA（with the highest yield reaching 12.39 μg/mL）.Under the conditions of initial pH 8.0，9.0，and 10.0 of LB medium，the strain Bachu42 could reduce the pH value of the medium by 8.75%，12.78% and 20.10%，respectively.The growth promotion effect was most significant under the condition of pH 8.0+2.0 mmol/L NaHCO3 in the tomato plate growth‐promotion test.Compared with the control，the aerial fresh weight，underground fresh weight，total fresh weight，lateral root number and leaf number of tomato inoculated with Bachu42 increased by 81.26%，160.04%，96.37%，144.88% and 26.40%，respectively.In the pot experiment of maize，the strain Bachu42 had the best growth promotion effect under 15 g/kg saline‐alkaline stress.Compared with the control group，the growth rates of aerial fresh weight，underground fresh weight，total fresh weight and plant height were 95.77%，56.27%，76.99% and 38.16%，respectively，after inoculating the strain Bachu42.In conclusion，the strain Aidingimonas sp.Bachu42 isolated and screened in this study is a strain with saline‐tolerance，alkali reduction and growth‐promoting functions，and could be utilized as a microbial fertilizer strain resource for saline‐alkaline soil.

Key words: Plant growth‐promoting rhizobacteria（PGPR）, Saline‐alkaline soil, Aidingimonas, Growth‐promoting, Isolation and identification

摘要： 为给功能型微生物肥料研发和盐碱地改良提供菌种资源，从新疆维吾尔自治区巴楚县芦苇根际土壤中分离耐盐碱植物促生根际细菌（Plant growth‐promoting rhizobacteria，PGPR），采用高盐高碱LB培养基富集培养耐盐碱微生物，结合形态学观察和16S rRNA基因测序确定其分类地位，使用不同NaCl质量浓度和不同pH 值的LB 培养基测定菌株的盐碱耐受性和降碱能力，采用不同功能鉴定培养基及Salkowski比色法检测菌株固氮、解有机磷、溶无机磷、产1-氨基环丙烷-1-羧酸（ACC）脱氨酶、产铁载体和产吲哚-3-乙酸（IAA）等植物促生相关特性，并通过平皿和盆栽试验评估菌株促生能力。结果表明，筛选获得了1 株Aidingimonas属菌株，将其命名为Aidingimonas sp.Bachu42，专利保藏号为CGMCCNo.26952。菌株Bachu42可分别在NaCl质量浓度介于20~200 g/L和pH值介于7.0~11.0的条件下生长。该菌株具有固氮、解有机磷、溶无机磷的功能，且可以产铁载体、ACC脱氨酶和IAA（最高产量达12.39 μg/mL）。在初始pH值分别为8.0、9.0、10.0的LB培养基条件下，菌株Bachu42可使培养基的pH值分别降低8.75%、12.78%、20.10%。在不同盐碱胁迫条件下，菌株Bachu42对番茄和玉米均有促生效果。其中，在番茄平皿促生试验pH值为8.0+2.0 mmol/L NaHCO₃的条件下，促生效果最为显著，和对照相比，接种菌株Bachu42后番茄的地上鲜质量、地下鲜质量、总鲜质量、侧根数和叶片数分别提高了81.26%、160.04%、96.37%、144.88%、26.40%；玉米盆栽试验中，在15 g/kg的盐碱胁迫条件下，菌株Bachu42的促生效果最好，相比于对照，玉米的地上鲜质量、地下鲜质量、总鲜质量和株高增长率分别为95.77%、56.27%、76.99%、38.16%。综上，分离筛选的菌株Aidingimonas sp.Bachu42具有耐盐碱、降碱和促生功能，可作为盐碱地功能型微生物肥料菌种资源利用。

关键词: 植物促生根际细菌（PGPR）, 盐碱地, Aidingimonas, 促生, 分离鉴定