Effects of Chitosan Oligosaccharides with Different Molecular Weight on Growth and Physiological Characteristics of Maize Seedlings under Drought Stress

doi:10.15933/j.cnki.1004-3268.2024.12.002

Abstract

Abstract: Under drought stress，50 mg/L chitosan oligosaccharides with different molecular weight （1 197，1 428，1 990 u）were sprayed to explore the effects of chitosan oligosaccharide with different molecular weight on the growth and physiological characteristics of maize seedlings，so as to provide a theoretical basis for the application of chitosan oligosaccharide with different molecular weight in production under drought stress.The results showed that under normal water condition，spraying chitosan oligosaccharide could promote the growth of maize seedlings. Under drought stress condition，spraying chitosan oligosaccharide could alleviate the impact of drought stress on the growth of maize seedlings.Among them，the plant height of treatments with chitosan oligosaccharides with small，medium and large molecular weight increased by 8.55%，9.37% and 5.80%，the stem diameter increased by 10.99%，5.23% and 1.31%，the dry weight of the aboveground part increased by 28.27%，38.82% and 19.83%，the dry weight of the underground part increased by 44.44%，39.58% and 29.17%，the leaf area increased by 43.45%，65.79% and 24.51%，the number of root branches increased by 114.57%，104.84% and 102.33%，the chlorophyll content increased by 43.07%，49.42% and 36.49%，the net photosynthetic rate（Pn）increased by 12.70%，22.49% and 6.80%，the content of superoxide anion decreased by 25.88%，35.74% and 21.83%，the activity of ascorbate peroxidase（APX）increased by 18.77%，29.92% and 11.43%，and the activity of glutathion peroxidase（GPX）increased by 23.30%，11.17% and 16.51%，respectively.Overall，under drought stress condition，spraying chitosan oligosaccharide with small molecular weight had the best effect on promoting the growth of the underground part，while spraying chitosan oligosaccharide with medium molecular weight had the best effect on promoting the growth of the aboveground part.The comprehensive evaluation results showed that the treatment with chitosan oligosaccharide with low molecular weight had the highest comprehensive score，followed by the treatment with chitosan oligosaccharide with medium molecular weight.

Key words: Chitosan oligosaccharide, Molecular weight, Maize, Drought stress, Growth and development, Physiological characteristics

摘要： 在干旱胁迫下，喷施50 mg/L不同分子质量（1 197、1 428、1 990 u）的壳寡糖，探究不同分子质量壳寡糖对玉米幼苗生长和生理特性的影响，为干旱胁迫下不同分子质量壳寡糖在生产中的应用提供理论依据。结果表明，正常水分条件下，喷施壳寡糖能促进玉米幼苗生长。干旱胁迫条件下，喷施壳寡糖能缓解干旱胁迫对玉米幼苗生长的影响。其中，小、中、大分子质量壳寡糖处理株高分别提高8.55%、9.37%、5.80%，茎粗分别增加10.99%、5.23%、1.31%，地上部干质量分别增加28.27%、38.82%、19.83%，地下部干质量分别提高44.44%、39.58%、29.17%，叶面积分别增加43.45%、65.79%、24.51%，根分枝数分别增加114.57%、104.84%、102.33%，叶绿素含量分别增加43.07%、49.42%、36.49%，净光合速率（Pn）分别提高12.70%、22.49%、6.80%，超氧阴离子含量分别减少25.88%、35.74%、21.83%，抗坏血酸过氧化物酶（APX）活性分别增加18.77%、29.92%、11.43%，谷胱甘肽过氧化物酶（GPX）活性分别增加23.30%、11.17%、16.51%。总体来看，干旱胁迫条件下喷施小分子质量壳寡糖对地下部生长的促进效果最好，中分子质量壳寡糖对地上部生长的促进效果最好。综合评价结果表明，喷施小分子质量壳寡糖处理综合得分最高，中分子质量壳寡糖处理次之。

关键词: 壳寡糖, 分子质量, 玉米, 干旱胁迫, 生长发育, 生理特性

CLC Number:

S513

ZHANG Le, ZHANG Fuguo, JIN Yazhong, ZHANG Yitao, ZHANG Youli. Effects of Chitosan Oligosaccharides with Different Molecular Weight on Growth and Physiological Characteristics of Maize Seedlings under Drought Stress[J]. Journal of Henan Agricultural Sciences, 2024, 53(12): 10-22.

张乐, 张富国, 靳亚忠, 张亦涛, 张有利. 不同分子质量壳寡糖对干旱胁迫下玉米幼苗生长和生理特性的影响[J]. 河南农业科学, 2024, 53(12): 10-22.

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