Journal of Henan Agricultural Sciences ›› 2025, Vol. 54 ›› Issue (5): 59-67.DOI: 10.15933/j.cnki.1004-3268.2025.05.007

• Agricultural Resources and Environment • Previous Articles     Next Articles

Screening of Cd‐tolerant Fungi with High Cd Removal Ability and Their Effects on Wheat Growth and Cd Accumulation

LI Shihang1,GAO Wei1,LI Yangyang1,SUN Shilong1,BAN Jiahao1,NIE Zhaojun1,GENG Yuehua2,WANG Min2,ZHAO Peng1   

  1. (1.College of Resources and Environment,Henan Agricultural University,Zhengzhou 450046,China;2.College of Plant Protection,Henan Agricultural University,Zhengzhou 450046,China)
  • Received:2024-08-29 Published:2025-05-15 Online:2025-06-09

高去除性耐镉真菌的筛选及其对小麦幼苗生长和镉积累的影响

李世行1,高巍1,李洋洋1,孙世龙1,班佳浩1,聂兆君1,耿月华2,汪敏2,赵鹏1   

  1. (1.河南农业大学 资源与环境学院,河南 郑州 450046;2.河南农业大学 植物保护学院,河南 郑州 450046)
  • 通讯作者: 高巍(1983-),女,山西太原人,副教授,博士,主要从事植物营养生态研究。E-mail:gaowei@henau.edu.cn 汪敏(1974-),男,河南信阳人,副教授,博士,主要从事植物病害综合防控研究。E-mail:wangm05@sina.com
  • 作者简介:李世行(2003-),男,河南许昌人,在读硕士研究生,研究方向:农业资源与环境。E-mail:lishihang2023@163.com
  • 基金资助:
    农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室项目(2024-cdhjwrfk-09);河南省高等学校重点科研项目(22A210018);河南省自然科学基金项目(242300420158)

Abstract: This study screened native fungi with high cadmium tolerance and removal performance from soil and plants contaminated with heavy metals in wheat fields,and produced solid fermentation remediation agents. Then it conducted wheat safety testing in healthy soil and investigated the effects of remediation agents on wheat growth and cadmium absorption under in‐situ heavy metal pollution conditions.The results showed that four functional fungi(M6,M10,M7,M3)with tolerance of up to 600 μmol/L Cd2+concentration and high cadmium removal rate in liquid culture medium containing 50 mg/L Cd2+were isolated and screened,with removal rates of 99.7%,79.3%,64.1%,and 62.8%,respectively.They were identified as Fusarium sp.,Trichoderma harzianumAlternaria sp. ,and Chaetomium sp..In wheat safety testing,only M6 was found to inhibit the emergence rate and biomass of wheat. Under in‐situ heavy metal contaminated soil,the addition of M3,M7,and M10 significantly increased the fresh weight (22.0%—43.7%)and dry weight(15.7%—64.4%)of wheat,meanwhile significantly reducing the accumulation of Cd in the wheat root(37.2%—53.4%).Among them,M7 treatment had the best effect.In summary,Chaetomium sp.M7 could promote the growth of wheat seedlings under cadmium stress and reduce cadmium accumulation,which could be used for soil heavy metal pollution control.

Key words: Wheat, Fungi, Heavy metal, Cadmium, Microbial remediation

摘要: 从镉污染小麦田土壤和植株中筛选耐镉(Cd)、去除镉性能较优的本土真菌,并制作固体发酵物修复剂,在健康土壤中进行小麦安全性检测,探究原位重金属污染条件下修复剂对小麦生长和镉吸收的影响。结果表明,共分离筛选出4株耐受程度高达600 μmol/L Cd2+浓度、在含Cd2+质量浓度为50 mg/L的液体培养基中去镉率较高的功能真菌(M6、M10、M7、M3),去除率分别为99.7%、79.3%、64.1%、62.8%,经鉴定分别为镰刀菌属(Fusarium sp.)、哈茨木霉(Trichoderma harzianum)、毛壳属(Chaetomium sp.)和链格孢属(Alternaria sp.)。小麦安全性检测中,仅处理M6抑制小麦的出苗率和生物量。原位重金属污染土壤下,添加M3、M7和M10显著增加小麦的鲜质量(22.0%~43.7%)、干质量(15.7%~64.4%),显著降低小麦根系对Cd的积累(37.2%~53.4%),其中,M7处理效果最佳。综上,毛壳属M7可以促进小麦幼苗在镉胁迫下的生长并降低镉积累,具有用于土壤重金属污染治理的潜力。

关键词: 小麦, 真菌, 重金属, 镉, 微生物修复

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