Preliminary Study on Molecular Mechanism of Drought Tolerance of Maize Inbred Line Xin 4095 Based on miRNA‐mRNA Integration Analysis Technology

doi:10.15933/j.cnki.1004-3268.2026.01.004

Abstract

Abstract: To explore the molecular mechanism of maize response to drought stress，drought‐tolerant maize inbred line Xin 4095 was used as the experimental material. Normal watering group（control）and drought treatment group were set up. When drought‐induced leaf yellowing and mild curling were observed，photosynthetic parameters were measured.Meanwhile，high‐throughput sequencing technology was used for microRNA（miRNA）and mRNA sequencing to screen differentially expressed miRNAs and mRNAs，the differentially expressed miRNA‐target gene regulatory network was constructed，and GO and KEGG enrichment analysis of differentially expressed mRNAs and target genes of differentially expressed miRNAs were done.The results showed that after drought treatment，the transpiration rate，net photosynthetic rate and stomatal conductance of inbred line Xin 4095 significantly decreased，while the water use efficiency significantly increased.A total of 42 differentially expressed miRNAs were identified，among which 28 miRNAs were up‐regulated and 14 miRNAs were down‐regulated.A total of 2 299 differentially expressed mRNAs were obtained，including 1 294 mRNAs up‐regulated and 1 005 mRNAs down‐regulated. Under drought stress，the differentially expressed mRNAs were significantly enriched in 20 GO terms，mainly including response to heat，photosynthesis，photosystem Ⅰ，chloroplast photosystem Ⅱ ，chloroplast photosystem Ⅰ ，thylakoid，cytosolic large ribosomal subunit，structural constituent of ribosome，unfolded protein binding，ribosome assembly and translation，protein refolding，etc.Differentially expressed mRNAs were significantly enriched in 20 KEGG pathways，mainly including photosynthesis，ribosome，protein processing in endoplasmic reticulum and other pathways.A differentially expressed miRNA‐target gene regulatory network was constructed，which included a total of 31 differentially expressed miRNAs（20 miRNAs were up‐regulated and 11 miRNAs were down‐regulated）.The target genes of differentially expressed miRNAs were significantly enriched in oxidative stress response related items such as response to oxidative stress and hydrogen peroxide catabolic process，protein phosphorylation related items such as protein serine/threonine kinase activity，and chloroplast function related items such as chloroplast envelope；they were significantly enriched in KEGG pathways such as plant hormone signal transduction，MAPK signaling pathway‐plant，biosynthesis of secondary metabolites，henylpropanoid biosynthesis，glutathione metabolism，starch and sucrose metabolism，alpha‐linolenic acid metabolism，linoleic acid metabolism，ascorbate and aldarate metabolism and so on.

Key words: Maize, Drought stress, Drought tolerance, microRNA, mRNA, Transcriptome

摘要： 为探究玉米响应干旱胁迫的分子机制，以耐旱玉米自交系新4095为试验材料，设置正常浇水组（对照）和干旱处理组，待观察到干旱诱导叶片黄化和轻度卷曲时，测定光合指标，同时利用高通量测序技术进行microRNA（miRNA）和mRNA测序，筛选差异表达miRNA和mRNA，构建差异表达miRNA-靶标基因调控网络，并对差异表达mRNA和差异表达miRNA的靶标基因进行GO和KEGG富集分析。结果表明，干旱处理后，玉米自交系新4095的蒸腾速率、净光合速率和气孔导度均极显著降低，而水分利用效率显著提高。共鉴定出42个差异表达miRNA，其中，28个上调、14个下调；共获得2 299个差异表达mRNA，其中，1 294个上调、1 005个下调。干旱胁迫下差异表达mRNA显著富集于20个GO条目，主要包括热响应、光合作用、光系统Ⅰ、叶绿体光系统Ⅱ、叶绿体光系统Ⅰ、类囊体、胞质大亚基核糖体、核糖体结构成分、未折叠蛋白质结合、核糖体组装与翻译、蛋白质重折叠等条目；显著富集于20个KEGG通路，主要包括光合作用、核糖体、内质网蛋白质加工等通路。构建了差异表达miRNA-靶标基因调控网络，该调控网络中共包含31个差异表达miRNA（20个上调、11个下调）。差异表达miRNA的靶标基因显著富集于氧化胁迫响应、过氧化氢分解过程等氧化应激响应相关条目，蛋白质丝氨酸/苏氨酸激酶活性等蛋白质磷酸化相关条目，叶绿体被膜等叶绿体功能相关条目；显著富集于植物激素信号转导、植物MAPK信号通路、次生代谢物生物合成、苯丙烷类生物合成、谷胱甘肽代谢、淀粉和蔗糖代谢、α-亚麻酸代谢、亚油酸代谢、抗坏血酸和醛酸盐代谢等KEGG通路。

关键词: 玉米, 干旱胁迫, 耐旱性, microRNA, mRNA, 转录组

CLC Number:

S513

WEI Xiaoyi, LIU Zhicheng, ZHANG Zhanhui, SHI Dakun, LI Fangjie, HONG Defeng, SUN Pei, LI Zhi, WEI Feng. Preliminary Study on Molecular Mechanism of Drought Tolerance of Maize Inbred Line Xin 4095 Based on miRNA‐mRNA Integration Analysis Technology[J]. Journal of Henan Agricultural Sciences, 2026, 55(1): 40-51.

卫晓轶, 刘志成, 张战辉, 史大坤, 李方杰, 洪德峰, 孙佩, 李知, 魏锋. 基于miRNA-mRNA 整合分析技术的玉米自交系新4095 耐旱分子机制初探[J]. 河南农业科学, 2026, 55(1): 40-51.

Figures/Tables 10

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