Genome‐Wide Identification of IDL Gene Family in Maize and Its Expression Analysis during Root Development

doi:10.15933/j.cnki.1004-3268.2026.01.005

Abstract

Abstract: The IDL［IDA（inflorescence deficient in abscission）‐like］gene family members were identified in maize at the whole‐genome level，and their physicochemical properties，phylogenetic evolution，and cis‐elements in promoter region were analyzed.The expression patterns of ZmIDL genes in different regions（meristem zone，elongation zone，maturation zone） and tissues（stele，cortex） of the primary root，as well as under hormone and drought treatments were detected using quantitative real‐time PCR（qRT‐PCR），which would lay a foundation for revealing the role of IDL gene in maize root development.The results showed that seven ZmIDL genes（ZmIDL1—7）were identified，classified into three subgroups and distributed across six chromosomes without collinearity.Their promoter regions contained cis‐elements in response to hormones and abiotic stresses.Expression analysis revealed that ZmIDL genes were highly expressed in the root cortex，pericarp|aleurone and silk，and responded to multiple abiotic stresses including drought，low/high temperature，salt and UV.qRT‐PCR analysis revealed that ZmIDL1/3/4/6 expression levels were significantly higher in Zhengdan 958 root cortex than those in stele，while ZmIDL2 and ZmIDL7 expression levels were higher in stele；ZmIDL5 did not express.As the germination time extended，ZmIDL1 expression level gradually increased in maturation zone，meristem zone and elongation zone of primary root of Xianyu 335，while ZmIDL6 expression level increased only in the maturation zone of primary root of Xianyu 335，with no significant change in meristem zone and elongation zone of primary root of Zhengdan 958 and Xianyu 335.When auxin transport was blocked in the maturation zone of primary root，ZmIDL1/6 expression level significantly increased，but significantly decreased after transport recovery.Under early drought stress（16 h for Zhengdan 958，8 h for Xianyu 335），ZmIDL6 and ZmIDL1 expression levels increased in the maturation zone of primary root.These findings suggest that ZmIDL6 and ZmIDL1 may synergistically regulate maize root development and respond to drought stress.

Key words: Maize, IDL genes, Genome‐wide identification, Root development, Evolutionary analysis, Gene expression, Auxin

摘要： 在全基因组水平鉴定玉米IDL［IDA（Inflorescence deficient in abscission）‐like］基因家族成员，分析其理化性质、系统进化和启动子顺式作用元件，并通过实时荧光定量PCR（qRT-PCR）技术检测ZmIDL基因在玉米主根的不同部位（分生区、伸长区、成熟区）、不同组织（中柱、皮层）及激素、干旱处理下的表达模式，为揭示IDL 基因在玉米根系发育中的作用奠定基础。结果表明，鉴定出7 个ZmIDL 基因（ZmIDL1—7），分属于3个亚组，7个ZmIDL基因分布在6条染色体上，且彼此之间不存在共线性关系，其启动子区域存在激素、非生物胁迫响应顺式作用元件。表达模式数据分析结果表明，ZmIDL基因在根部皮层、种子糊粉层和花丝中高表达，并且响应干旱、低温、高温、盐、UV等多种非生物胁迫。qRT-PCR结果显示，郑单958根皮层中ZmIDL1/3/4/6基因的表达量极显著高于中柱，而ZmIDL2和ZmIDL7基因则在中柱中表达量更高，ZmIDL5基因没有表达；随着萌发时间延长，ZmIDL1基因表达量在先玉335主根成熟区、分生区和伸长区均逐渐提高，ZmIDL6基因表达量仅在先玉335主根成熟区逐渐提高，在先玉335和郑单958主根分生区和伸长区无明显变化。当主根成熟区生长素转运受阻时，ZmIDL1/6基因表达量显著升高，而转运恢复后其表达量显著降低。此外，ZmIDL6和ZmIDL1基因表达量在干旱胁迫初期玉米（郑单958 16 h、先玉335 8 h）主根成熟区提高。推测ZmIDL6 和ZmIDL1 基因可能协同调控玉米根系发育，并响应干旱胁迫。

关键词: 玉米, IDL基因, 全基因组鉴定, 根系发育, 进化分析, 基因表达, 生长素

CLC Number:

S513

LI Yanhua, LI Chunyang, WU Xiaolin, WANG Wei, LIU Hui. Genome‐Wide Identification of IDL Gene Family in Maize and Its Expression Analysis during Root Development[J]. Journal of Henan Agricultural Sciences, 2026, 55(1): 52-64.

李艳华, 李春阳, 吴晓林, 王伟, 刘辉. 玉米IDL 基因家族的全基因组鉴定及在根系发育中的表达分析[J]. 河南农业科学, 2026, 55(1): 52-64.

Figures/Tables 9

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