Creation and Phenotypic Analysis of TaGW2‑A1‑Overexpression Transgenic Wheat Germplasm

doi:10.15933/j.cnki.1004-3268.2025.01.001

Abstract

Abstract: The expression vector of TaGW2‑A1 was constructed，and transformed into wheat；T₂ homozygous transgenic lines were identified and screened；the expression of TaGW2‑A1 gene of the homozygous transgenic line was analyzed by real‑time fluorescent quantitative PCR and Western blot；subcellular localization analysis was performed by tobacco transient expression and cell component separation；the agronomic traits and quality traits of homozygous lines were analyzed，so as to lay foundation for further analysis of TaGW2 regulatory network，and provide gene resource for breeding new wheat varieties with high yield and quality.The results showed that the expression vector of TaGW2‑A1 gene was successfully constructed，positive transgenic plants were obtained，and homozygous transgenic lines were screened in T₂ generation.The transcription level of TaGW2‑A1 was significantly higher in leaves and grains of homozygous transgenic lines，but TaGW2‑A1 protein was detected only in grains.Subcellular localization results showed that TaGW2‑A1 protein mainly functioned in cytoplasm and nucleus. Phenotypic analysis results showed that TaGW2-A1 overexpression significantly reduced plant height，grain length，grain width，1 000‑grain weight and yield per plant，but had no significant effect on grain number per spike and grain protein content.

Key words: TaGW2-A1, Wheat, Thousand?grain weight, Grain width, Overexpression

摘要： 构建TaGW2-A1基因过表达载体并转化小麦，鉴定并筛选T2纯合株系，通过实时荧光定量PCR和Western blot试验分析TaGW2-A1基因及其编码蛋白质表达情况，结合烟草瞬时表达和细胞组分分离试验进行亚细胞定位分析，并对转基因纯合株系进行表型分析，为深入解析TaGW2调控网络奠定基础，并为小麦高产优质新品种的培育提供基因资源。结果表明，成功构建TaGW2-A1基因过表达载体，转化小麦后获得阳性转基因植株，并在T₂筛选获得纯合转基因株系。TaGW2-A1基因表达水平在转基因纯合株系叶片和籽粒中较高，但仅在籽粒中检测到TaGW2-A1蛋白。亚细胞定位结果表明，TaGW2-A1蛋白主要在细胞质和细胞核中行使功能。表型分析结果表明，TaGW2-A1基因过表达显著降低株高、粒长、粒宽、千粒质量和单株产量，而对穗粒数和籽粒蛋白质含量没有显著影响。

关键词: TaGW2-A1, 小麦, 千粒质量, 粒宽, 过表达

CLC Number:

S512

ZHANG Haoran, LIU Xiaoying, ZHOU Fenglong, XU Mingchen, GUO Zhengzheng, CHENG Jiayu, ZHANG Kunpu, WANG Daowen, SHI Cuilan. Creation and Phenotypic Analysis of TaGW2‑A1‑Overexpression Transgenic Wheat Germplasm[J]. Journal of Henan Agricultural Sciences, 2025, 54(1): 1-8.

张浩然, 刘晓莹, 周峰龙, 许明晨, 郭争争, 程佳雨, 张坤普, 王道文, 师翠兰. TaGW2-A1 过表达小麦种质的创制及表型分析[J]. 河南农业科学, 2025, 54(1): 1-8.

Figures/Tables 7

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