关键词: 高粱, 脂氧合酶基因家族, 基因结构, 进化关系, 表达模式

Abstract: Bioinformatics technology was used to identify lipoxygenase(LOX) family genes in sorghum,and their evolutionary relationship,gene structure,conservative motif and expression patterns in different tissues and under low temperature stress were analyzed,so as to lay a foundation for the functional study of sorghum LOX gene. The results showed that 11 sorghum LOX genes were identified,named SbLOX1—SbLOX11 according to their chromosomal positions,which could be divided into two subfamilies, and the gene structure of the same subgroup was basically similar.The gene expression profiles analysis of sorghum LOX family in various plant tissues revealed that expression patterns of different LOX genes varied. The expression levels of SbLOX10 and SbLOX9 were lower in most tissues,whereas the expression levels of SbLOX2—SbLOX5 and SbLOX8 were higher in almost all different tissues. The transcriptome data of LOX genes were analyzed under low temperature (4℃ ),the expression levels of SbLOX1,SbLOX3,SbLOX5 in 9-LOX subgroup and SbLOX4,SbLOX9 in 13-LOX subgroup of cold tolerant sorghum were upregulated,and which of cold sensitive sorghum were higher too, speculating that LOX gene had important role in response to low temperature stress.

Key words: Sorghum bicolor;Lipoxygenase(LOX)gene family;Gene structure;Evolutionary relationship, Expression pattern