欧洲葡萄VvCAX 基因家族的鉴定与表达分析

doi:10.15933/j.cnki.1004-3268.2023.11.013

摘要/Abstract

摘要： CAX（Ca²⁺/H⁺exchanger antiporter）是一类重要的阳离子转运蛋白，在植物生长发育和抵抗非生物胁迫中至关重要。通过生物信息学分析的方法，从欧洲葡萄中共鉴定出5个VvCAX基因，根据其在染色体上的位置依次命名为VvCAX1—VvCAX5，进行了包括理化性质、二级结构、三级结构、跨膜结构域、基因结构、保守基序、系统进化树等分析。结果表明，VvCAX基因家族成员均为疏水蛋白，主要定位于质膜和液泡，包含10~11个跨膜结构域和4个典型的功能域，α-螺旋和无规则卷曲在二级结构中占据主要位置，所有成员三级结构非常相似且均为单体。系统发育分析将VvCAX分为2个亚家族，同一亚家族的VvCAX结构相似，不同亚家族VvCAX的保守基序有差异，且7对VvCAX基因在进化过程中发生了基因的片段复制事件。VvCAX的互作蛋白主要与钙离子转运、钙离子通道和离子转运相关。上游调控因子和启动子顺式作用元件分析结果表明，VvCAX基因可能通过MYB、ERF等转录因子的调控参与激素和胁迫响应，这一推论经表达模式分析得到了验证。通过不同胁迫下的表达分析发现，所有VvCAX基因的表达水平均受ABA的影响，ⅠA亚家族的成员响应创伤、霜霉菌、干旱和低温，而ⅠB亚家族的成员主要与高温有关，ⅠA亚家族的VvCAX基因更有研究价值。

关键词: 欧洲葡萄, VvCAX基因家族, 基因结构, 胁迫响应, 表达分析

Abstract: CAX（Ca²⁺/H⁺exchanger antiporter）is an important cation transporter，which is essential for plant growth and development and resistance to abiotic stress.In our study，a total of five VvCAX genes were identified from Vitis vinifera by bioinformatics analysis，named VvCAX1—VvCAX5 in turn according to the position of each member on the chromosomes，and their physical and chemical properties，secondary structure，tertiary structure，transmembrane domain，gene structure，conserved motif，and phylogenetic tree were analyzed.The results showed that members of the VvCAX gene family were all hydrophobins，which were mainly localized to plasma membrane and vacuole，and contained 10—11 transmembrane domains and four typical functional domains.The secondary structure was dominated by α⁃helix and random coil，and the tertiary structure was very similar and monomer.Phylogenetic analysis showed that VvCAX was divided into two subfamilies，and the structure of VvCAX in the same subfamily was similar，while the conserved motifs of VvCAX in different subfamilies were not alike.Seven pairs of VvCAX genes underwent fragment replication events during the evolution of grapes.The interaction proteins of VvCAX were mainly related to calcium transport，calcium channel，and ion transport.The analysis of upstream regulatory factors and promoter cis⁃acting elements showed that the VvCAX genes might be involved in hormone and stress response by the regulation of transcription factors such as MYB and ERF，which was verified by expression pattern analysis.The expression levels of all VvCAX genes were affected by ABA.The members of the ⅠA subfamily responded to wound，downy mildew，drought，and low temperature，while the members of the ⅠB subfamily were mainly related to high temperature，so VvCAX genes of the ⅠA subfamily were more valuable for further research.

Key words: Vitis vinifera, VvCAX gene family, Gene structure, Stress response, Expression analysis

中图分类号:

S663.1

崔小月, 尚泓泉, 吕中伟, 娄玉穗, 张柯. 欧洲葡萄VvCAX 基因家族的鉴定与表达分析[J]. 河南农业科学, 2023, 52(11): 113-123.

CUI Xiaoyue, SHANG Hongquan, LÜ Zhongwei, LOU Yusui, ZHANG Ke. Identification，Characterization，and Expression Analysis of VvCAX Gene Family in Grapevine[J]. Journal of Henan Agricultural Sciences, 2023, 52(11): 113-123.

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