关键词: 菊花, SVP基因, 基因结构, 光周期处理, 温度处理, 表达模式, 花期调控

Abstract: As an important flowering inhibitor,SHORT VEGETATIVE PHASE(SVP) gene is affected by multiple environmental factors, and participates in multiple flowering regulation pathways.In order to clarify the function of CmSVP gene in the regulation of Chrysanthemum flowering,CmSVP gene of the dayneutral Chrysanthemum morifolium Jinbudiao was cloned by PCR,and the gene structure was analyzed.The expression patterns of CmSVP in different tissues in different periods,under different photoperiods and at different temperatures were detected by qRT-PCR.The results showed that the total length of CmSVP gDNA was 4 997 bp,which consisted of 8 exons and 7 introns.The expression level of CmSVP in the vegetative period was higher than that in the reproductive growth period.The expression abundance was highest at the shoot tip,and that of the inflorescence was the lowest.So it was speculated that CmSVP might inhibit flower formation.The CmSVP gene showed sensitivity to photoperiod and temperature,especially the dual effects of long day and low temperature could significantly inhibit its expression.These results indicated that the CmSVP was similar in structure to the SVP homologous genes of other species,and was mainly expressed in the vegetative period.It was speculated to inhibit flowering transition through temperature-sensitive pathways and photoperiod pathways.

Key words: Chrysanthemum morifolium, SVP gene, Gene structure, Photoperiod treatment, Temperature treatment, Expression pattern, Flowering regulation