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Study on the Methods for Overcoming Interspecific Hybridization Barriers in Tulipa
- ZHANG Yanqiu, XING Guimei, CUI Yuehan, YANG Di, QU Lianwei
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2021, 50(8):
124-132.
DOI: 10.15933/j.cnki.1004-3268.2021.08.015
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In order to overcome the interspecific hybridization barriers in Tulipa,and improve the seed setting rate,the interspecific hybridization was performed between the Tulipa wild species Tulipa altaica and three Tulipa cultivars Eskimo Chief,World’s Favorite and Strong Gold.The effects of different pollination treatments and different plant growth regulators on cross‑compatibility were studied.The results showed that the seed setting rate of interspecific hybridization in Tulipa was greatly influenced by different cross combinations and treatments. Under normal pollination condition,the ovary of the two
crossing combinations with World’s Favorite and Strong Gold as female parents could not swell,while the ovary swelling rate was 20.00% with Eskimo Chief as female parent,but no seeds were obtained.Bud pollination was not obvious to overcome the barrier of interspecific hybridization,and the ovary of three hybrid combinations could not swell and no seed was obtained. Delaying pollination,scratching stigma pollination,plant growth regulator treatments etc. had positive effects on overcoming the interspecific hybridization barriers. When diploid cultivar Eskimo Chief was used as female parent,26,19 hybrid seeds were obtained by delaying pollination and scratching stigma pollination treatments respectively,with the seed setting rate of 13.33%,6.67%,and the embryo rate of 42.31%,21.05% respectively.When the triploid cultivars World’s Favorite and Strong Gold were used as female parents,no seed was obtained despite a certain proportion of enlargement in all the treated ovaries,indicating that the interspecific hybridization between different ploidy had more serious hybridization barrier.