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Journal of Henan Agricultural Sciences    2013, 42 (4): 179-183.   Abstract （143）      PDF （815KB）（1726）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (2): 16-18.   Abstract （410）      PDF （143KB）（1451）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2015, 44 (2): 91-93,100.   Abstract （36）      PDF （31024KB）（1248）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (12): 20-24.   Abstract （187）      PDF （223KB）（1245）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2012, 41 (2): 9-11,53.   Abstract （112）      PDF （278KB）（1223）       Knowledge map    Save Related Articles | Metrics

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Research Progress on Cold Resistance of Wheat FANG Yuhui, HAN Liupeng, HUA Xia, ZHAO Mingzhong, GUO Rui, QI Xueli, HU Lin Journal of Henan Agricultural Sciences    2022, 51 (4): 1-10.   DOI: 10.15933/j.cnki.1004-3268.2022.04.001 Abstract （763）      PDF （1275KB）（1218）       Knowledge map    Save The freeze injury is a frequent natural disaster in wheat production，which is one of the important environmental factors restricting wheat yield and quality. In this paper，the type of wheat freeze injury，the physiological mechanism（cell membrane system，osmotic adjustment substances，antioxidant system，photosynthetic characteristics and endogenous hormones）and molecular mechanisms（cold resistant genes，cold resistant omics） of wheat cold resistance were summarized. The problems and solutions in the study of wheat cold resistance were also discussed，which provided an important reference for the research of cold resistance mechanism and breeding of wheat. Reference | Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2010, 39 (4): 115-118.   DOI: 10.3969/j.issn.1004-3268.2010.04.032 Abstract （437）      PDF （211KB）（1189）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2019, 48 (8): 1-6.   Abstract （1608）      PDF （843KB）（1187）       Knowledge map    Save Reference | Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2005, 34 (1): 9-12.   DOI: 10.3969/j.issn.1004-3268.2005.01.002 Abstract （473）      PDF （141KB）（1040）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2012, 41 (8): 1-5.   Abstract （98）      PDF （206KB）（1023）       Knowledge map    Save Related Articles | Metrics

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Expression and Purification of CRISPR/Cas13a and Establishment of Its Collateral RNase Activity Assay WANG Xun, ZHANG Yuhang, SUN Yaning, LI Qingmei, LI Ge, WANG Li, GUO Junqing, DENG Ruiguang, ZHANG Gaiping Journal of Henan Agricultural Sciences    2021, 50 (4): 147-153.   DOI: 10.15933/j.cnki.1004-3268.2021.04.019 Abstract （496）      PDF （4597KB）（964）       Knowledge map    Save To estabish LwaCas13a molecular detection platform,LwaCas13a was expressed in E.coli and its collateral RNase activity was determined.The recombinant plasmid pET His6-TwinStrep-SUMOLwaCas13a was transformed into Rosetta(DE3) competent cells to induce expression.The results of SDSPAGE electrophoresis and Western blot analysis showed that the soluble LwaCas13a recombinant protein was successfully expressed. Optimization of inducing temperature and time showed that maximal production of LwaCas13a was obtained at 15 h after induction at 16℃ .The expressed LwaCas13a recombinant protein was purified by nickel column affinity chromatography to obtain a single target band with ideal purification effect.In addition,the collateral RNase assay was also established with in vitro synthesized CRISPR RNA and target RNA.The assay could be performed within 30 min at constant 37℃ ,with the detection limit of 31.2 nmol/L.To sum up,the LwaCas13a was successfully expressed in E.coli Rosetta (DE3).Purified LwaCas13a exhibited ideal collateral RNase activity,and the collateral RNase assay for LwaCas13a was optimized and established. Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2012, 41 (12): 20-23.   Abstract （97）      PDF （401KB）（958）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2013, 42 (12): 11-15.   Abstract （163）      PDF （226KB）（949）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (11): 17-21.   Abstract （161）      PDF （229KB）（941）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2006, 35 (4): 9-12.   DOI: 10.3969/j.issn.1004-3268.2006.04.002 Abstract （440）      PDF （145KB）（929）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2013, 42 (1): 106-109.   Abstract （130）      PDF （176KB）（906）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2013, 42 (7): 1-5.   Abstract （111）      PDF （311KB）（887）       Knowledge map    Save Related Articles | Metrics

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Research Progress on Plant Phenomics in the Context of Smart Agriculture YANG Wenqing, LIU Tianxia, TANG Xingping, XU Guofu, MA Zhe, YANG Hekai, WU Wendou Journal of Henan Agricultural Sciences    2022, 51 (7): 1-12.   DOI: 10.15933/j.cnki.1004-3268.2022.07.001 Abstract （692）      PDF （1262KB）（885）       Knowledge map    Save China’s agriculture is in the key stage of changing from traditional agriculture to modern agriculture.Smart agriculture is an important embodiment of modern agricultural development and an inevitable trend of agricultural development in the future.Smart agriculture aims to deeply combine modern information technologies such as internet of things，artificial intelligence and big data with traditional agriculture to make agricultural production intelligent，green，standardized and digital.Plant phenomics is a science to study the characteristics of plant phenotypes，which is one of the key technologies for the development of smart agriculture.By collecting phenotypic data at all levels of cells，organs，tissues，plants and populations，plant phenomics extracts important trait information with high repeatability and high reliability from massive data，so as to provide data support and method support for gene mining，crop breeding and accurate management of agricultural production process.This paper reviews the development status of plant phenomics under the background of smart agriculture from the aspects of phenotypic data collection and analysis and the construction of plant phenotypic analysis platform at home and abroad，summarizes the application of plant phenomics research in smart agricultural production，and finally looks forward to the future development trend of plant phenomics. Reference | Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (12): 30-34.   Abstract （108）      PDF （224KB）（867）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (12): 113-119.   Abstract （163）      PDF （288KB）（852）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2014, 43 (5): 114-117.   Abstract （224）      PDF （198KB）（852）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2005, 34 (5): 30-32+45.   DOI: 10.3969/j.issn.1004-3268.2005.05.010 Abstract （108）      PDF （140KB）（849）       Knowledge map    Save Related Articles | Metrics

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Review on Activation and Regulation of CBF to Mediate Low-temperature Response in Plants ZHAO Junli, CHEN Wen, SHI Min Journal of Henan Agricultural Sciences    2020, 49 (4): 1-6.   DOI: 10.15933/j.cnki.1004-3268.2020.04.001 Abstract （337）      PDF （1131KB）（840）       Knowledge map    Save Low temperature not only limits the growth and development of plants,but also limits the distribution of plants.To reduce the damage induced by low-temperature stress,plants evolve many mechanisms to resist cold stress through changing gene expression level and cellular environmental homeostasis.CBF(C-repeat binding transcription factor) plays critical roles in response to low temperature stress,and improves cold tolerance by altering the expression of cold regulated genes(COR).The functions of CBF and downstream genes regulated by CBF,the upstream genes that regulated CBF gene,and the interaction between CBF and hormones were reviewed,and the application prospect of CBF in agricultural production was raised.This review can lay the foundation for future study of crop cold tolerance mechanism,and provide theoretical reference for breeding of cold tolerant crops by gene engineering. Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (3): 125-128.   Abstract （184）      PDF （143KB）（836）       Knowledge map    Save Related Articles | Metrics

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Progress on Nutritional Value of Schizochytrium limacinum and Its Application in Animal Production ZHANG Xinming, ZHAO Lu, HU Xiaowen Journal of Henan Agricultural Sciences    2021, 50 (3): 1-10.   DOI: 10.15933/j.cnki.1004-3268.2021.03.001 Abstract （387）      PDF （1261KB）（817）       Knowledge map    Save Schizochytrium limacinum is rich in nutritional value,especially essential fatty acids such as DHA required for animal growth,so it has good application prospects in animal production.The basic nutritional value and biologically active substances of Schizochytrium limacinum were reviewed,and the influence of environmental factors on the production of Schizochytrium limacinum DHA was analyzed.The application of Schizochytrium limacinum in improving the growth performance and survival rate of animals,increasing the nutritional value of products,and promoting the fat metabolism and improving non-specific immunity of the body was reviewed.The application prospects of Schizochytrium limacinum in animal feed and additives were prospected, aiming to provide reference for the development and application of Schizochytrium limacinum. Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2014, 43 (2): 1-5.   Abstract （140）      PDF （244KB）（814）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (3): 19-24.   Abstract （103）      PDF （511KB）（813）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2010, 39 (7): 74-76.   DOI: 10.3969/j.issn.1004-3268.2010.07.021 Abstract （208）      PDF （209KB）（800）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2012, 41 (2): 26-32.   Abstract （200）      PDF （316KB）（799）       Knowledge map    Save Related Articles | Metrics

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Analysis of Combining Ability and Heredity for Plant Yield Traits in Pepper HAN Ya’nan, CHANG Xiaoke, CHENG Zhifang, ZHANG Tao, YAO Qiuju Journal of Henan Agricultural Sciences    2021, 50 (7): 136-144.   DOI: 10.15933/j.cnki.1004-3268.2021.07.016 Abstract （339）      PDF （6392KB）（798）       Knowledge map    Save In order to evaluate the combining ability and heredity for plant architecture and yield components，and improve the efficiency of selecting elite combinations of pepper（Capsicum annuum L.）,five parental lines（C1—C5）with different characters were used to develop 20 hybrid crosses by Griffing complete diallel crossing design，and nine agronomic traits of 25 genetypes were determined to analyze heterosis，combining ability and heritability．The results showed that the heterosis indexes of yield per plant，fruit number per plant，plant width and fruit length were higher，with the averages of up to 145.98%，131.35% 122.51% and 121.20%，respectively. The coefficients of variation of yield per plant and fruit number per plant were 31.80% and 17.78%，respectively. Analysis of combination ability showed that C2 and C4 with high general combination ability（GCA）were the optimal lines in single fruit weight and single plant yield，and the crosses C4×C5，C1×C2，C2×C3 and C4×C5，C3×C2 were determined as the excellent combinations in weight and single plant yield respeitively based on special combination ability（SCA）and reciprocal（REC）effects. The high values of broad⁃sense heritability and narrow⁃sense heritability for yield per plant and fruit width indicated that these traits were mainly controlled by additive effects，and the environmental variances of stem diameter and flesh thickness were higher than their genetic variances. The coefficients between major traits and yield per plant suggested that the yield per plant was positively correlated with single fruit weight，fruit length and fruit number per plant. In conclusion，the parental lines C2,C4 and the crosses C3×C2，C4×C5 are suitable for cultivating highly⁃yielding varieties. Pepper breeding should be focused on single fruit weight，fruit length and fruit number per plant. Table and Figures | Reference | Related Articles | Metrics

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Research Progress of CTX-M Type Extended-spectrum β-lactamases LIU Baoguang, LI Yucheng, WANG Baoying, BAI Ming , MIAO Mingsan, XU Erping Journal of Henan Agricultural Sciences    2019, 48 (12): 1-7.   DOI: 10.15933/j.cnki.1004-3268.2019.12.001 Abstract （352）      PDF （1136KB）（798）       Knowledge map    Save The production of extended-spectrum β-lactamases(ESBLs) is the main mechanism of bacterial resistance to β-lactamide antibiotics. In recent years,with the widespread use of cephalosporins,the spread of CTX-M ESBLs has become strong,causing popularity and spread in many countries and regions.It has gradually become the main type of ESBLs epidemic in many countries,and poses a great threat to human and animal health. In view of this,the discovery,species,biochemical characteristics,epidemiology and therapeutic measures of this enzyme strain in recent years are reviewed in this paper.The gene mutation,transfer,translocation factors and genetic environment of gene transfer of this enzyme strain are summarized,so as to provide references for the control of multidrug-resistant enzyme-producing bacteria. Reference | Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2010, 39 (4): 119-125.   DOI: 10.3969/j.issn.1004-3268.2010.04.033 Abstract （121）      PDF （221KB）（795）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2014, 43 (3): 47-52.   Abstract （141）      PDF （933KB）（795）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (9): 30-33.   Abstract （255）      PDF （199KB）（791）       Knowledge map    Save Related Articles | Metrics

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Genetic Structure Analysis of Parental Inbred Lines of Main Maize Hybrid Varieties in China LI Jingjing, ZHANG Wenyang, WANG Lifeng, WANG Hao, LI Huiyong Journal of Henan Agricultural Sciences    2021, 50 (1): 27-34.   DOI: 10.15933/j.cnki.1004-3268.2021.01.004 Abstract （254）      PDF （2995KB）（791）       Knowledge map    Save Genetic structure of parental inbred lines of the common maize varieties was analyzed using 56K maize gene chip,and heterosis modes of parental inbred lines of the main maize varieties were analyzed,so as to provide guidance and reference for maize breeding.The results showed that a total of 35 261 high-quality single nucleotide polymorphism(SNP) markers were obtained from 56 000 SNPs,the average of minimum allele frequency (MAF) was 0.28,the MAF of 12.56% of the SNP markers were 0.45—0.50,the polymorphism level was high.Polymorphic information content(PIC) was 0.10—0.50,with an average of 0.37.The results of cluster analysis showed that,five groups were divided,of which,group Ⅰ was mainly BSSS germplasm,which was represented by PH6WC;group Ⅱ mainly contained PH4CV,male parents of hybrid DK517 and DH605 and so on; group Ⅲ contained Reid, P group germplasm and European male parents;group Ⅳ contained domestic lines,such as Zong 3 and Zong 31;group Ⅴ contained classic SPT germplasm,Suwan and CIMMYT tropical germplasm.At the same time,the parental SNP differences of 16 maize hybrids were analyzed,and a total of 7 615 polymorphic sites and 7 592 conserved sites were selected. Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2013, 42 (1): 1-4.   Abstract （63）      PDF （200KB）（780）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2010, 39 (6): 141-143.   DOI: 10.3969/j.issn.1004-3268.2010.06.036 Abstract （180）      PDF （127KB）（779）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2010, 39 (6): 150-155.   DOI: 10.3969/j.issn.1004-3268.2010.06.039 Abstract （142）      PDF （192KB）（779）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2004, 33 (1): 33-36.   DOI: 10.3969/j.issn.1004-3268.2004.01.011 Abstract （164）      PDF （128KB）（778）       Knowledge map    Save Related Articles | Metrics

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Research Progress on Preparation and Application of Straw Wood Vinegar ZHAO Shuheng, LI Zhuojie, LIU Xiaofeng, HU Jianjun, DING Yanbo Journal of Henan Agricultural Sciences    2022, 51 (1): 1-10.   DOI: 10.15933/j.cnki.1004-3268.2022.01.001 Abstract （539）      PDF （1429KB）（769）       Knowledge map    Save Wood vinegar，as a valuable acidic by‐product in the process of biomass pyrolysis for carbon production，is rich in various organic compounds such as acids，alcohols，phenols，aldehydes，esters，ethers and a small amount of metal elements，which has a high application value.In the raw material of wood vinegar，because of low lignin content and poor quality in straw，the development and application of straw wood vinegar are slow.However，the study of straw wood vinegar is of great significance to the utilization of agricultural waste and has a broad development prospect.In order to provide references for the further development and efficient utilization of straw wood vinegar，the crude preparation and refining technology of straw wood vinegar were introduced，the composition and physicochemical properties of straw wood vinegar were summarized，and the research and application progresses of straw wood vinegar in agriculture，medicine and health and other fields were reviewed. Table and Figures | Reference | Related Articles | Metrics