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Journal of Henan Agricultural Sciences    2013, 42 (4): 179-183.   Abstract （168）      PDF （815KB）（2165）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (2): 16-18.   Abstract （452）      PDF （143KB）（1777）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (12): 20-24.   Abstract （209）      PDF （223KB）（1563）       Knowledge map    Save Related Articles | Metrics

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Research Progress on Cold Resistance of Wheat FANG Yuhui, HAN Liupeng, HUA Xia, ZHAO Mingzhong, GUO Rui, QI Xueli, HU Lin Journal of Henan Agricultural Sciences    2022, 51 (4): 1-10.   DOI: 10.15933/j.cnki.1004-3268.2022.04.001 Abstract （981）      PDF （1275KB）（1424）       Knowledge map    Save The freeze injury is a frequent natural disaster in wheat production，which is one of the important environmental factors restricting wheat yield and quality. In this paper，the type of wheat freeze injury，the physiological mechanism（cell membrane system，osmotic adjustment substances，antioxidant system，photosynthetic characteristics and endogenous hormones）and molecular mechanisms（cold resistant genes，cold resistant omics） of wheat cold resistance were summarized. The problems and solutions in the study of wheat cold resistance were also discussed，which provided an important reference for the research of cold resistance mechanism and breeding of wheat. Reference | Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2005, 34 (7): 48-53.   DOI: 10.3969/j.issn.1004-3268.2005.07.017 Abstract （71）      PDF （200KB）（1359）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2010, 39 (4): 115-118.   DOI: 10.3969/j.issn.1004-3268.2010.04.032 Abstract （505）      PDF （211KB）（1346）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2012, 41 (2): 9-11,53.   Abstract （141）      PDF （278KB）（1314）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2013, 42 (1): 106-109.   Abstract （144）      PDF （176KB）（1291）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2015, 44 (2): 91-93,100.   Abstract （42）      PDF （31024KB）（1256）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2019, 48 (8): 1-6.   Abstract （1786）      PDF （843KB）（1250）       Knowledge map    Save Reference | Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2012, 41 (8): 1-5.   Abstract （113）      PDF （206KB）（1241）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2005, 34 (1): 9-12.   DOI: 10.3969/j.issn.1004-3268.2005.01.002 Abstract （715）      PDF （141KB）（1239）       Knowledge map    Save Related Articles | Metrics

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Research Progress on Plant Phenomics in the Context of Smart Agriculture YANG Wenqing, LIU Tianxia, TANG Xingping, XU Guofu, MA Zhe, YANG Hekai, WU Wendou Journal of Henan Agricultural Sciences    2022, 51 (7): 1-12.   DOI: 10.15933/j.cnki.1004-3268.2022.07.001 Abstract （753）      PDF （1262KB）（1238）       Knowledge map    Save China’s agriculture is in the key stage of changing from traditional agriculture to modern agriculture.Smart agriculture is an important embodiment of modern agricultural development and an inevitable trend of agricultural development in the future.Smart agriculture aims to deeply combine modern information technologies such as internet of things，artificial intelligence and big data with traditional agriculture to make agricultural production intelligent，green，standardized and digital.Plant phenomics is a science to study the characteristics of plant phenotypes，which is one of the key technologies for the development of smart agriculture.By collecting phenotypic data at all levels of cells，organs，tissues，plants and populations，plant phenomics extracts important trait information with high repeatability and high reliability from massive data，so as to provide data support and method support for gene mining，crop breeding and accurate management of agricultural production process.This paper reviews the development status of plant phenomics under the background of smart agriculture from the aspects of phenotypic data collection and analysis and the construction of plant phenotypic analysis platform at home and abroad，summarizes the application of plant phenomics research in smart agricultural production，and finally looks forward to the future development trend of plant phenomics. Reference | Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2013, 42 (12): 11-15.   Abstract （208）      PDF （226KB）（1221）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2013, 42 (7): 1-5.   Abstract （151）      PDF （311KB）（1211）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2005, 34 (5): 30-32+45.   DOI: 10.3969/j.issn.1004-3268.2005.05.010 Abstract （157）      PDF （140KB）（1197）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (12): 30-34.   Abstract （126）      PDF （224KB）（1179）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2005, 34 (1): 31-34.   DOI: 10.3969/j.issn.1004-3268.2005.01.009 Abstract （168）      PDF （148KB）（1172）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2014, 43 (9): 110-115.   Abstract （41）      PDF （738KB）（1170）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2010, 39 (6): 150-155.   DOI: 10.3969/j.issn.1004-3268.2010.06.039 Abstract （154）      PDF （192KB）（1132）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (11): 17-21.   Abstract （208）      PDF （229KB）（1096）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2008, 37 (10): 110-112,115.   DOI: 10.3969/j.issn.1004-3268.2008.10.032 Abstract （24）      PDF （246KB）（1077）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2006, 35 (4): 9-12.   DOI: 10.3969/j.issn.1004-3268.2006.04.002 Abstract （554）      PDF （145KB）（1071）       Knowledge map    Save Related Articles | Metrics

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Expression and Purification of CRISPR/Cas13a and Establishment of Its Collateral RNase Activity Assay WANG Xun, ZHANG Yuhang, SUN Yaning, LI Qingmei, LI Ge, WANG Li, GUO Junqing, DENG Ruiguang, ZHANG Gaiping Journal of Henan Agricultural Sciences    2021, 50 (4): 147-153.   DOI: 10.15933/j.cnki.1004-3268.2021.04.019 Abstract （558）      PDF （4597KB）（1055）       Knowledge map    Save To estabish LwaCas13a molecular detection platform,LwaCas13a was expressed in E.coli and its collateral RNase activity was determined.The recombinant plasmid pET His6-TwinStrep-SUMOLwaCas13a was transformed into Rosetta(DE3) competent cells to induce expression.The results of SDSPAGE electrophoresis and Western blot analysis showed that the soluble LwaCas13a recombinant protein was successfully expressed. Optimization of inducing temperature and time showed that maximal production of LwaCas13a was obtained at 15 h after induction at 16℃ .The expressed LwaCas13a recombinant protein was purified by nickel column affinity chromatography to obtain a single target band with ideal purification effect.In addition,the collateral RNase assay was also established with in vitro synthesized CRISPR RNA and target RNA.The assay could be performed within 30 min at constant 37℃ ,with the detection limit of 31.2 nmol/L.To sum up,the LwaCas13a was successfully expressed in E.coli Rosetta (DE3).Purified LwaCas13a exhibited ideal collateral RNase activity,and the collateral RNase assay for LwaCas13a was optimized and established. Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (12): 113-119.   Abstract （207）      PDF （288KB）（1046）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2010, 39 (4): 119-125.   DOI: 10.3969/j.issn.1004-3268.2010.04.033 Abstract （148）      PDF （221KB）（1043）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences

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Journal of Henan Agricultural Sciences    2012, 41 (12): 20-23.   Abstract （116）      PDF （401KB）（1028）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2009, 38 (8): 101-104.   DOI: 10.3969/j.issn.1004-3268.2009.08.027 Abstract （50）      PDF （147KB）（1011）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2014, 43 (5): 114-117.   Abstract （251）      PDF （198KB）（998）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2005, 34 (2): 42-46.   DOI: 10.3969/j.issn.1004-3268.2005.02.013 Abstract （40）      PDF （169KB）（988）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2013, 42 (3): 112-114.   Abstract （89）      PDF （734KB）（978）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2005, 34 (5): 88-92.   DOI: 10.3969/j.issn.1004-3268.2005.05.029 Abstract （106）      PDF （167KB）（974）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2004, 33 (11): 70-72.   DOI: 10.3969/j.issn.1004-3268.2004.11.022 Abstract （91）      PDF （109KB）（970）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2012, 41 (2): 26-32.   Abstract （250）      PDF （316KB）（963）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2011, 40 (3): 125-128.   Abstract （248）      PDF （143KB）（960）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2007, 36 (2): 20-23.   DOI: 10.3969/j.issn.1004-3268.2007.02.005 Abstract （339）      PDF （156KB）（959）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2012, 41 (1): 11-15.   Abstract （154）      PDF （222KB）（948）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2014, 43 (5): 6-12.   Abstract （185）      PDF （288KB）（943）       Knowledge map    Save Related Articles | Metrics

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Journal of Henan Agricultural Sciences    2014, 43 (2): 1-5.   Abstract （190）      PDF （244KB）（940）       Knowledge map    Save Related Articles | Metrics