Effects of Plasma Activated Water on Oat Seed Germination and Seedling Growth

doi:10.15933/j.cnki.1004-3268.2026.02.004

Abstract

Abstract: Using distilled water without plasma treatment as the control（CK），atmospheric pressure argon cold plasma at 2 and 3 kV was used to activate distilled water for 5，10，and 15 min，resulting in six types of plasma‑activated water（PAW）. These types of PAW were then used to irrigate oat seeds to investigate the effects of PAW on oat seed germination and seedling growth. A comprehensive evaluation of different PAW treatments was conducted using the membership function method and principal component analysis，so as to provide a theoretical basis for the application of PAW in enhancing the growth potential of oat seeds. The results showed that the NO₂⁻ content of F（PAW produced by 3 kV atmospheric pressure argon cold plasma treatment for 15 min） treatment was the highest. Compared with CK，all PAW treatments generally improved the germination vigor，germination rate，germination index，and vigor index of oat seeds，with E（PAW produced by 3 kV atmospheric pressure argon cold plasma treatment for 10 min）showing the greatest increase. E and F treatments also improved seedling plant height，root length，number of rootlets，fresh weight，dry weight，and contents of chlorophyll，carotenoid，and soluble protein，with E treatment being more effective. Comprehensive evaluation indicated that E treatment had the best promoting effect on oat seed germination and seedling growth，followed by F treatment.In conclusion，PAW produced by 3 kV atmospheric pressure argon cold plasma treatment for 10 min has the best promoting effect on oat seed germination and seedling growth.

Key words: Oat, Plasma activated water, Seed germination, Seedling growth, Principal component analysis

摘要： 以未经过等离子体处理的蒸馏水为对照（CK），分别采用2 kV和3 kV的大气压氩气冷等离子体对蒸馏水进行5、10、15 min的活化处理，制备得到6种等离子体活化水（PAW），并用于培养燕麦种子，探究PAW对燕麦种子萌发及幼苗生长的影响，并采用隶属函数法及主成分分析法对不同PAW处理进行综合评价，以期为PAW在燕麦种子生长潜力提升方面的应用提供理论依据。结果表明，PAW中NO₂^-含量以F（3 kV大气压氩气冷等离子体处理15 min制备的PAW）处理最高。与CK相比，所有PAW处理燕麦种子的发芽势、发芽率、发芽指数和活力指数总体上均提高，以E（3 kV的大气压氩气冷等离子体处理10 min制备的PAW）处理提高幅度最大；E和F处理幼苗的株高、根长、根须数、鲜质量、干质量及叶绿素、类胡萝卜素、可溶性蛋白含量均提高，以E处理效果更好。综合评价结果表明，E处理对燕麦种子萌发和幼苗生长的促进效果最好，其次是F处理。综上，3 kV的大气压氩气冷等离子体处理10 min制备的PAW对燕麦种子萌发和幼苗生长的促进效果最佳。

关键词: 燕麦, 等离子体活化水, 种子萌发, 幼苗生长, 主成分分析

CLC Number:

S512.6

WU Furong, BAO Jin, LÜ Xiaogui. Effects of Plasma Activated Water on Oat Seed Germination and Seedling Growth[J]. Journal of Henan Agricultural Sciences, 2026, 55(2): 29-36.

吴富荣, 包锦, 吕晓桂. 等离子体活化水对燕麦种子萌发及幼苗生长的影响[J]. 河南农业科学, 2026, 55(2): 29-36.

Figures/Tables 10

References

［1］任长忠，闫金婷，董锐，等. 燕麦营养成分、功能特性及其产品的研究进展［J］. 食品工业科技，2022，43（12）：438‑446.
REN C Z，YAN J T，DONG R，et al. Research progress on oat nutrients，functional properties and related products［J］.Science and Technology of Food Industry，2022，43（12）：438‑446.
［2］陈强，熊先勤，尚以顺，等. 贵州高寒牧区引进苏特燕麦种植技术要点及现状分析［J］. 现代畜牧科技，2023（10）：63‑66.
CHEN Q，XIONG X Q，SHANG Y S，et al. Analysis on the key points and current situation of introducing shooter oat planting technology in alpine pastoral area of Guizhou［J］. Modern Animal Husbandry Science & Technology，2023（10）：63‑66.
［3］BIBI R，ELAHI N N，DANISH S，et al. Enhancing germination and growth of canola（Brassica napus L.）through hydropriming and NaCl priming［J］. Scientific Reports，2024，14：14026.
［4］LUJAN ROCHA R，KHALIL Y，MAITY A，et al.Mechanical scarification technique breaks seed coat‑mediated dormancy in wild oat（Avena fatua）［J］.Weed Technology，2022，36（1）：152‑159.
［5］卜咏梅，包锦，吕晓桂. 冷等离子体处理对燕麦种子发芽及幼苗抗旱性的影响［J］.农业工程学报，2024，40（22）：243‑251.
BU Y M，BAO J，LÜ X G. Effects of cold plasma treatment on oat seeds germination and seeding drought resistance［J］.Transactions of the Chinese Society of Agricultural Engineering，2024，40（22）：243‑251.
［6］熊作明，杨佳欢，张克亮. 紫叶加拿大紫荆种子休眠与萌发特性研究［J］. 安徽农业科学，2024，52（14）：73‑75.
XIONG Z M，YANG J H，ZHANG K L. Research on dormancy and germination characteristics of Cercis canadensis‘forest pansy’seeds［J］. Journal of Anhui Agricultural Sciences，2024，52（14）：73‑75.
［7］季晓莲，马港振，戴钊，等. 红豆杉种子休眠原因及其解除技术研究［J］. 西北林学院学报，2024，39（4）：112‑119.
JI X L，MA G Z，DAI Z，et al. Studies on dormancy mechanism and dormancy release of Taxus wallichiana var.chinensis［J］.Journal of Northwest Forestry University，2024，39（4）：112‑119.
［8］唐林，王松，郭柯宇，等. 低温等离子体活化水在食品杀菌保鲜中的应用［J］. 中国食品学报，2021，21（12）：347‑357.
TANG L，WANG S，GUO K Y，et al. The application of low‑temperature plasma activated water in food sterilization and preservation［J］. Journal of Chinese Institute of Food Science and Technology，2021，21（12）：347‑357.
［9］GURAGAIN R P，PRADHAN S P，BANIYA H B，et al.Impact of plasma‑activated water（PAW）on seed germination of soybean［J］. Journal of Chemistry，2021，2021（1）：7517052.
［10］GIERCZIK K，VUKUŠIĆ T，KOVÁCS L，et al.Plasma‑activated water to improve the stress tolerance of barley［J］. Plasma Processes and Polymers，2020，17（3）：1900123.
［11］RASHID M，RASHID M M，REZA M A，et al.Combined effects of air plasma seed treatment and foliar application of plasma activated water on enhanced paddy plant growth and yield［J］. Plasma Chemistry and Plasma Processing，2021，41（4）：1081‑1099.
［12］齐先科，李淼，李彩虹，等. PAW及其与LTP的联合作用对小麦种子萌发及幼苗生长的影响［J］. 河南农业科学，2024，53（4）：20‑29.
QI X K，LI M，LI C H，et al. Effects of PAW and its combined action with LTP on seed germination and seedling growth of wheat［J］.Journal of Henan Agricultural Sciences，2024，53（4）：20‑29.
［13］SIVACHANDIRAN L，KHACEF A. Enhanced seed germination and plant growth by atmospheric pressure cold air plasma：Combined effect of seed and water treatment［J］. RSC Advances，2017，7（4）：1822‑1832.
［14］PRIATAMA R A，SONG I，LEE Y K. Enhancing oat growth using plasma‑activated water treatment［J］.Journal of Asian Agriculture and Biotechnology，2023，39（2）：13‑26.
［15］蔡庆生. 植物生理学实验［M］. 北京：中国农业大学出版社，2013.
CAI Q S. Plant physiology experiments［M］. Beijing：China Agricultural University Press，2013.
［16］王孝平，邢树礼. 考马斯亮蓝法测定蛋白含量的研究［J］. 天津化工，2009，23（3）：40‑42.
WANG X P，XING S L. Determination of protein quantitation using the method of coomassie brilliant blue［J］. Tianjin Chemical Industry，2009，23（3）：40‑42.
［17］THAN H A Q，PHAM T H，NGUYEN D K V，et al.Non‑thermal plasma activated water for increasing germination and plant growth of Lactuca sativa L［J］.Plasma Chemistry and Plasma Processing，2022，42（1）：73‑89.
［18］LINDSAY A，BYRNS B，KING W，et al. Fertilization of radishes，tomatoes，and marigolds using a large‑volume atmospheric glow discharge［J］. Plasma Chemistry and Plasma Processing，2014，34（6）：1271‑1290.
［19］刘定新，何桐桐，张浩. 等离子体对人体组织的渗透作用：研究现状与前沿问题［J］. 高电压技术，2019，45（7）：2329‑2342.
LIU D X，HE T T，ZHANG H. Penetration effect of gas plasmas on human tissues：State‑of‑the‑art and current issues［J］. High Voltage Engineering，2019，45（7）：2329‑2342.
［20］吴穷，王豪，张丽阳，等. 等离子体产物溶液中扩散与大肠杆菌杀灭［J］. 高电压技术，2021，47（3）：826‑831.
WU Q，WANG H，ZHANG L Y，et al. Plasma reactive species diffusion and the inactivation of bacteria［J］.High Voltage Engineering，2021，47（3）：826‑831.
［21］ESUA O J，CHENG J H，SUN D W. Functionalization of water as a nonthermal approach for ensuring safety and quality of meat and seafood products［J］. Critical Reviews in Food Science and Nutrition，2021，61（3）：431‑449.
［22］MILDAZIENE V，IVANKOV A，SERA B，et al.Biochemical and physiological plant processes affected by seed treatment with non‑thermal plasma［J］. Plants，2022，11（7）：856.
［23］BORMASHENKO E，GRYNYOV R，BORMASHENKO Y，et al. Cold radiofrequency plasma treatment modifies wettability and germination speed of plant seeds［J］. Scientific Reports，2012，2：741.
［24］TACHIBANA K，NAKAMURA T. Comparative study of discharge schemes for production rates and ratios of reactive oxygen and nitrogen species in plasma activated water［J］. J Phys D Appl Phys，2019，52（38）：385202.
［25］王虹，阎君，赵志勇，等. 低温等离子体活化水对菠菜种子萌发及幼苗生长的影响［J］. 上海蔬菜，2023（3）：93‑96.
WANG H，YAN J，ZHAO Z Y，et al. Effects of low temperature plasma activated water on seed germination and seedling growth of spinach［J］.Shanghai Vegetables，2023（3）：93‑96.
［26］WOJTYLA Ł，LECHOWSKA K，KUBALA S，et al.Different modes of hydrogen peroxide action during seed germination［J］. Frontiers in Plant Science，2016，7：66.
［27］KUČEROVÁ K，HENSELOVÁ M，SLOVÁKOVÁ Ľ，et al. Effects of plasma activated water on wheat：Germination， growth parameters， photosynthetic pigments，soluble protein content，and antioxidant enzymes activity［J］.Plasma Processes and Polymers，2019，16（3）：1800131.

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