Effects of Extracellular Polysaccharides on Seed Germination，Seedling Growth and Physiological Characteristics of Schizonepeta tenuifolia under Salt Stress

doi:10.15933/j.cnki.1004-3268.2024.10.011

Abstract

Abstract: In order to improve the planting efficiency of Schizonepeta tenuifolia in saline soil,the effects of extracellular polysaccharides from Lactobacillus plantarum on seed germination and seedling growth under salt stress were explored，and the specific mechanism was analyzed from the physiological characteristics of seedling roots.Six treatments were set up:blank control group(CK1),salt stress control group(CK2),50 mg/kg extracellular polysaccharide group(T1),100 mg/kg extracellular polysaccharide group(T2),200 mg/kg extracellular polysaccharide group(T3),and 400 mg/kg extracellular polysaccharide group(T4).The results showed that extracellular polysaccharides significantly promoted the seed germination and seedling growth of Schizonepeta tenuifolia.Among them,the effect of adding 200 mg/kg extracellular polysaccharide(T3)was the most significant.Compared with CK2,the seed germination rate，germination potential,germination index and vigor index were significantly increased by 76.13%,144.16%,137.45% and 265.28%,respectively.The plant height,stem diameter,nderground dry weight and aboveground dry weight of seedlings were significantly increased by 60.49%,14.73%,40.08% and 85.39%,respectively.The contents of proline,soluble protein and soluble sugar were increased by 21.02%，84.56% and 40.02%,respectively.The content of Na+ was decreased by 54.63%,the K+ content was increased by 95. 24%，and the Na+/K+ value decreased by 76.76%.The root electrolyte leakage rate and malondialdehyde（MDA） content decreased by 44.54% and 43.24%,respectively,and the activities of superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD)increased by 30.39%,34.59% and 24.31%,respectively.In summary,the extracellular polysaccharides of Lactobacillus plantarum can improve the salt resistance of seedlings through root osmotic regulation and activation of antioxidant enzyme defense system,thereby promoting seed germination and seedling growth of Schizonepeta tenuifolia.

Key words: Schizonepeta tenuifolia, Lactobacillus plantarum, Extracellular polysaccharide, Seed germination, Seedling growth, Physiological characteristic

摘要： 为提高盐渍地中荆芥的种植效益，探究植物乳杆菌（Lactobacillus plantarum）胞外多糖对盐胁迫下荆芥种子萌发、幼苗生长的影响，同时从幼苗根系生理特性上分析其具体作用机制，设置6个处理［空白对照组（CK1）、盐胁迫对照组（CK2）、50 mg/kg胞外多糖组（T1）、100 mg/kg胞外多糖组（T2）、200 mg/kg胞外多糖组（T3）、400 mg/kg胞外多糖组（T4）］进行试验。结果表明，胞外多糖对荆芥种子萌发和幼苗生长具有显著的促进作用。其中，添加200 mg/kg胞外多糖（T3）效果最显著，与CK2相比，其种子发芽率、发芽势、发芽指数和活力指数分别显著增加76.13%、144.16%、137.45%、265.28%，幼苗株高、茎粗、地下干质量和地上干质量分别显著升高60.49%、14.73%、40.08%、85.39%，脯氨酸、可溶性蛋白、可溶性糖含量分别提高21.02%、84.56%、40.02%，Na+含量降低54.63%，K+含量升高95.24%，Na+/K+值降低76.76%，根系电解质渗出率、丙二醛（MDA）含量分别降低44.54%、43.24%，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）活性分别显著提高30.39%、34.59%、24.31%。综上所述，植物乳杆菌胞外多糖可以通过根系渗透调节和激活抗氧化酶防御系统来提高幼苗的抗盐性，进而促进荆芥种子萌发和幼苗生长。

关键词: 荆芥, 植物乳杆菌, 胞外多糖, 种子萌发, 幼苗生长, 生理特性

CLC Number:

TANG Qian, WANG Jiali, LI Wenqiang. Effects of Extracellular Polysaccharides on Seed Germination，Seedling Growth and Physiological Characteristics of Schizonepeta tenuifolia under Salt Stress[J]. Journal of Henan Agricultural Sciences, 2024, 53(10): 96-105.

唐茜, 王佳丽, 李文强. 胞外多糖对盐胁迫下荆芥种子萌发、幼苗生长及生理特性的影响[J]. 河南农业科学, 2024, 53(10): 96-105.

References

［1］马凯，饶良懿.我国土壤盐碱化问题研究脉络和热点分析［J］.中国农业大学学报，2023，28（11）：90⁃102.
MA K，RAO L Y.Research lineage and hot spot analysis of soil salinization in China［J］.Journal of China Agricultural University，2023，28（11）：90⁃102.
［2］潘晶，王磊，黄翠华，等.盐胁迫对干旱区植物能量代谢过程的影响综述［J］.中国沙漠，2024，44（1）：111⁃118.
PAN J，WANG L，HUANG C H，et al.Review on effects of salt stress on plant energy metabolism processes in arid regions［J］.Journal of Desert Research，2024，44（1）：111⁃118.
［3］张晨，戴良香，张冠初，等.旱盐复合胁迫对花生荚果发育特性、产量和品质的影响［J］.花生学报，2023，52（1）：72⁃79.
ZHANG C，DAI L X，ZHANG G C，et al.Effects of drought⁃salt combined stress on pod development，yield and quality of peanut［J］.Journal of Peanut Science，2023，52（1）：72⁃79.
［4］盛玉章.水盐胁迫及施肥对甘草和秦艽品质的影响［D］.杨凌：西北农林科技大学，2022.
SHENG Y Z.Effects of water and salt stress and fertilization on the quality of Glycyrrhiza uralensis Fisch and Gentiana macrophylla Maxim.［D］.Yangling：Northwest A & F University，2022.
［5］KANG S M，SHAFFIQUE S，INJAMUM⁃UL⁃HOQUE M，et al.Foliar treatment with melatonin modulates photosynthetic and antioxidant responses in Silybum marianum L.under salt stress［J］.Scientia Horticulturae，2024，325：112664.
［6］JIADKONG K，FAUZIA A N，YAMAGUCHI N，et al.Exogenous riboflavin（vitamin B2）application enhances salinity tolerance through the activation of its biosynthesis in rice seedlings under salinity stress［J］.Plant Science，2024，339：111929.
［7］曹依静，逯昀，赵莉霞，等.腐殖酸对盐胁迫下山定子砧苹果嫁接苗的缓解效应［J］.江苏农业科学，2023，51（8）：124⁃131.
CAO Y J，LU Y，ZHAO L X，et al.Alleviative effect of humic acid on grafted seedlings of Malus mandshurica under salt stress［J］.Jiangsu Agricultural Sciences，2023，51（8）：124⁃131.
［8］张克鑫.嗜根寡养单胞菌JC1及其胞外多糖对Pb2+胁迫下苜蓿生长的影响［D］.兰州：兰州理工大学，2023.
ZHANG K X.Effects of Stenotrophomonas rhizophilus JC1 and its extracellular polysaccharide on alfalfa growth under Pb2+ stress［D］.Lanzhou：Lanzhou University of Technology，2023.
［9］LIU X T，YAO T，CHAI J L，et al.Adsorption of sodium ions by exopolysaccharides from Pseudomonas simiae MHR6 and its improvement of Na⁺/K⁺ homeostasis in maize under salt stress［J］.Journal of Agricultural and Food Chemistry，2023，71（50）：19949⁃19957.
［10］HIDRI R，METOUI⁃BEN MAHMOUD O，ZORRIG W，et al.The halotolerant rizhobacterium Glutamicibacter sp.alleviates salt impact on Phragmites australis by producing exopolysaccharides and limiting plant sodium uptake［J］.Plant Direct，2023，7（10）：e535.
［11］FATIMA T，ARORA N K. Structural，compositional characterization of novel exopolysaccharide from halotolerant strain of Pseudomonas entomophila PE3 and their role in growth promotion of sunflower under saline condition［J］.Environmental and Experimental Botany，2023，213：105418.
［12］王斌斌，吴宝美.假肠膜明串珠菌PC胞外多糖对逆境胁迫下水稻种子萌发的影响［J］.江苏农业科学，2022，50（24）：59⁃64.
WANG B B，WU B M.Effect of exopolysaccharide from Leuconostoc pseudomesenteroides PC on seed germination of rice under different abiotic stresses［J］.Jiangsu Agricultural Sciences，2022，50（24）：59⁃64.
［13］张文平，杨臻，吴佩佳，等.乳酸菌胞外多糖对逆境胁迫下水稻种子萌发及幼苗生长的影响［J］.核农学报，2019，33（1）：138⁃147.
ZHANG W P，YANG Z，WU P J，et al.Effect of exopolysaccharides of lactic acid bacteria on seed germination and seedling growth of rice under different stress［J］.Journal of Nuclear Agricultural Sciences，2019，33（1）：138⁃147.
［14］梁锡稼.荆芥的栽培技术及药用价值［J］.河南农业，2022（7）：17.
LIANG X J.Cultivation techniques and medicinal value of Schizonepeta tenuifolia［J］.Agriculture of Henan，2022（7）：17.
［15］周莹，赵永娟，黄丽瑾，等.荆芥幼苗对盐胁迫的生理响应［J］.核农学报，2019，33（1）：166⁃175.
ZHOU Y，ZHAO Y J，HUANG L J，et al.Physiological responses of Schizonepeta tenuifolia Briq.seedlings to salt stress［J］.Journal of Nuclear Agricultural Sciences，2019，33（1）：166⁃175.
［16］张胜珍，马艳芝.氯化钙对盐胁迫下荆芥种子萌发及幼苗生理特性的影响［J］.中国农业科技导报，2021，23（7）：65⁃71.
ZHANG S Z，MA Y Z.Effects of CaCl2 on seed germination and seedling physiological characteristics of Schizonepeta tenuifolia Briq.under salt stress［J］.Journal of Agricultural Science and Technology，2021，23（7）：65⁃71.

［17］鲍士旦. 土壤农化分析［M］.3版. 北京：中国农业出版社，2000.

BAO S D.Soil agricultural analysis［M］.3rd ed.Beijing：China Agricultural Press，2000.

［18］陈建勋，王晓峰.植物生理学实验指导［M］.2版.广州：华南理工大学出版社，2006.
CHEN J X，WANG X F.Plant physiology experiment guide［M］.2nd ed.Guangzhou：South China University of Technology Press，2006.

［19］李合生，王学奎.现代植物生理学［M］.4版. 北京：高等教育出版社，2019.
LI H S，WANG X K. Modern plant physiology［M］.4th ed.Beijing：Higher Education Press，2019.

［20］王晓龙，闫利军.盐胁迫对3种披碱草种子萌发的影响［J］.草学，2023（6）：12⁃17.
WANG X L，YAN L J.Effects of salt stress on seed germination of three Elymus［J］.Journal of Grassland and Forage Science，2023（6）：12⁃17.

［21］朱广龙，武启迪，钱寅森，等.盐分胁迫对高粱生长与生理特征的影响及耐盐调控机理研究进展［J］.江苏农业科学，2023，51（14）：49⁃57.
ZHU G L，WU Q D，QIAN Y S，et al.Research progress on effects of salt stress on growth and physiological characteristics of Sorghum and regulation mechanism of salt tolerance［J］.Jiangsu Agricultural Sciences，2023，51（14）：49⁃57.

［22］张瑞娥，王娟，王智颖，等.NaCl胁迫对荆芥种子萌发及幼苗抗氧化酶活性的影响［J］.阜阳师范学院学报（自然科学版），2015，32（3）：59⁃64.
ZHANG R E，WANG J，WANG Z Y，et al.Effect of NaCl stress on germination and seedling’s antioxidant enzymes activity of Nepeta cataria L.［J］.Journal of Fuyang Teachers College（Natural Science），2015，32 （3）：59⁃64.

［23］刘凯月，郭玉洁，刘冬云.干旱和盐胁迫对3种地被植物种子萌发特性的影响［J］.林业与生态科学，2018，33（1）：88⁃92.
LIU K Y，GUO Y J，LIU D Y.Influences to the germination characteristics of three ground seeds under different stresses of drought and salt［J］.Forestry and Ecological Sciences，2018，33（1）：88⁃92.

［24］MORCILLO R J L，MANZANERA M.The effects of plant⁃associated bacterial exopolysaccharides on plant abiotic stress tolerance［J］.Metabolites，2021，11（6）：337.

［25］SUN K，LI Z，LIAN M Y，et al.Characterization of a novel exopolysaccharide from Acinetobacter rhizosphaerae with ability to enhance the salt stress resistance of rice seedlings［J］.International Journal of Biological Macromolecules，2024，256：128438.

［26］SUN L，CHENG L，MA Y H，et al.Exopolysaccharides from Pantoea alhagi NX⁃11 specifically improve its root colonization and rice salt resistance［J］.International Journal of Biological Macromolecules，2022，209：396⁃404.

［27］任新宇.植物乳杆菌胞外多糖对酸-镉复合胁迫下水稻幼苗生长及土壤环境的影响［D］.南昌：江西农业大学，2022.
REN X Y.Effects of extracellular polysaccharide from Lactobacillus plantarum on the growth of rice seedlings and soil environment under acid⁃cadmium combined stress［D］.Nanchang：Jiangxi Agricultural University，2022.

［28］JAVED S A，JAFFAR M T，SHAHZAD S M，et al.Optimization of nitrogen regulates the ionic homeostasis，potassium efficiency，and proline content to improve the growth，yield，and quality of maize under salinity stress［J］.Environmental and Experimental Botany，2024，226：105836.

［29］周莹.盐胁迫对荆芥生长生理及次生代谢的影响［D］.南京：南京农业大学，2019.
ZHOU Y.Effects of salt stress on growth physiology and secondary metabolism of Schizonepeta tenuifolia［D］.Nanjing：Nanjing Agricultural University，2019.

［30］周莹，黄丽瑾，赵永娟，等.花期荆芥在盐胁迫下离子吸收、分布和挥发油成分的变化［J］.中国中药杂志，2018，43（22）：4410⁃4418.
ZHOU Y，HUANG L J，ZHAO Y J，et al.Changes of ion absorption，distribution and essential oil components of flowering Schizonepeta tenuifolia under salt stress［J］.China Journal of Chinese Materia Medica，2018，43（22）：4410⁃4418.

［31］ARROUSSI H E，BENHIMA R，ELBAOUCHI A，et al.Dunaliella salina exopolysaccharides：A promising biostimulant for salt stress tolerance in tomato（Solanum lycopersicum）［J］.Journal of Applied Phycology，2018，30（5）：2929⁃2941.

［32］焦明翠，蔡立格，魏健，等.盐胁迫的生理危害与植物的适应机制研究进展［J］.长春师范大学学报，2023，42（6）：125⁃132.
JIAO M C，CAI L G，WEI J，et al.Research progress on the physiological harms of salt stress and the adaptation mechanism of plants［J］.Journal of Changchun Normal University，2023，42（6）：125⁃132.

［33］魏宏宇，李怡，彭帅英，等.胞外多糖促进胁迫条件下农作物生长的研究与展望［J］.江苏农业学报，2022，38（4）：1123⁃1134.
WEI H Y，LI Y，PENG S Y，et al.Promoting crop growth under stress conditions by exopolysaccharides：Review and perspective［J］.Jiangsu Journal of Agricultural Sciences，2022，38（4）：1123⁃1134.

［34］LIU X T，CHAI J L，ZHANG Y C，et al.Halotolerant rhizobacteria mitigate the effects of salinity stress on maize growth by secreting exopolysaccharides［J］.Environmental and Experimental Botany，2022，204：105098.

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