Abstract: The effects of plant growth‑promoting bacteria containing ACC deaminase on seed germination of Psammosilene tunicoides were studied，at the same time，the most suitable growth‑promoting strain was screened out to provide theoretical basis for the development and utilization of microbial fertilizer and high efficient cultivation of P.tunicoides.Four PGPB strains containing ACC deaminase and their mixed strains were used to impregnate P.tunicoides seeds and accelerate their germination.The germination rate，germination energy，germination index and daily average germination rate and so on were measured with sterile water as control.The results showed that，different PGPB strains containing ACC deaminase showed different effects on seed germination of P.tunicoides，among which F195 strain had the best germination effect. Compared with CK，seed germination rate，germination energy，germination index，daily average germination rate and vigor index increased by 32%，49%，223%，32% and 358%，respectively.The germination days of the first seed and peak days were advanced by 3 days and 2 days respectively，the minimum of average germination days was 8.30 d.The percentage of water absorption of seeds increased by 80%. Root length，germ length，seedling fresh weight and seedling dry weight increased by 63%，63%，76% and 131%，respectively.Z24 and Mix strains treatments had better germination effect，which were significantly different from CK（P<0.05）.The results of principal component analysis showed that the germ length，root length，germination rate，daily average germination rate and vigor index were higher in component 1，which could be used as important indexes to evaluate the germination and growth of P.tunicoides seeds under different PGPB strains.Cluster analysis showed that F195 strain had best germination effect，followed by Z24，Mix and Z39 strains.In conclusion，F195 strain has good growth‑promoting characteristics during seed germination.

Key words: Psammosilene tunicoides, ACC deaminase, Plant growth-promoting bacteria, Seed germination

摘要： 探究含ACC脱氨酶的植物促生菌（Plant growth‑promoting bacteria，PGPB）对金铁锁种子萌发的影响，同时筛选适宜的促生菌株，为微生物菌肥的开发利用及金铁锁的高效栽培提供理论依据。以金铁锁种子为试验材料，采用4株含ACC脱氨酶的PGPB菌株及其混合菌株对其进行浸种催芽试验，以无菌水为对照（CK），测定其发芽率、发芽势、发芽指数及日平均发芽率等指标。结果表明，含ACC脱氨酶的不同PGPB菌株对金铁锁种子萌发表现出不同效果。其中，F195菌株处理效果最佳，与CK相比，种子发芽率、发芽势、发芽指数、日平均发芽率和活力指数分别提高了32%、49%、223%、32%、358%；第1粒种子萌发天数、萌发高峰期天数分别提前了3、2 d，平均发芽天数最短为8.30 d；种子的吸水百分率增加80%；根系长度、胚芽长度、幼苗鲜质量和幼苗干质量分别增加了63%、63%、76%、131%。Z24和Mix菌株处理萌发效果次之，与CK相比均达到显著差异（P<0.05）水平。主成分分析结果表明，胚芽长度、根系长度、发芽率、日平均发芽率和活力指数在主成分1中载荷较高，可作为评价不同PGPB菌株对金铁锁种子萌发生长影响的重要指标。聚类分析结果表明，促进萌发效果较好的菌株为F195，其次为Z24、Mix和Z39菌株。综上，F195菌株在金铁锁种子萌发生长过程中具有良好的促生特性。

关键词: 金铁锁, ACC脱氨酶, 植物促生菌, 种子萌发