Abstract: Momordica charantia is a crop with significant economic and medicinal value. However，research on the distribution and stress resistance characteristics of the GATA gene family in Momordica charantia is still limited. This study identified and functionally analyzed the GATA gene family（McaGATA）in Momordica charantia through bioinformatics analysis. Results indicated that a total of 22McaGATA genes were identified，distributed across 14 chromosomes，and divided into 4 subfamilies based on phylogenetic analysis.The analysis of the physicochemical properties of McaGATA proteins showed that there were differences in the number of amino acids，molecular mass，isoelectric point，etc.，but they were all hydrophilic nuclear proteins.Cis⁃acting element analysis revealed that the promoter region of the McaGATA genes were rich in light response，hormone response，and stress response elements.qRT⁃PCR analysis indicated that McaGATA genes presented tissue⁃specific expression patterns in different tissues，and genes such as McaGATA9，McaGATA10，McaGATA15，McaGATA17 and McaGATA18 were induced by salt stress，showing significantly upregulated expression under low⁃concentration salt stress conditions.Furthermore，the yeast model was used to verify that McaGATA4，McaGATA15，McaGATA17 and McaGATA18 genes could significantly enhance the salt tolerance of yeast cells under low⁃concentration salt stress，while McaGATA18 showed the most prominent salt tolerance effect under high salt stress.

Key words: Momordica charantia, GATA gene family, Salt stress, Yeast

摘要： 金铃子是一种具有重要经济价值和药用价值的作物，有关金铃子GATA基因家族分布及其抗逆特性的研究还较为有限。通过生物信息学分析对金铃子GATA基因（McaGATA）家族进行了鉴定与功能分析。结果表明，共鉴定出22个McaGATAs 基因，分布于14条染色体上，且根据系统发育分析可将其分为4个亚家族。McaGATAs蛋白理化性质分析显示，其氨基酸数目、分子质量、等电点等存在差异，但均为亲水性的核蛋白。顺式作用元件分析显示，McaGATAs基因启动子区域富含光响应、激素响应及胁迫响应等元件。qRT-PCR分析表明，McaGATAs基因在不同组织中呈现出典型的组织表达特异性，并且McaGATA9、McaGATA10、McaGATA15、McaGATA17 和McaGATA18 等基因受到盐胁迫的诱导，在低浓度盐胁迫下表达量显著上调。此外，利用酵母模型验证了McaGATA4、McaGATA15、McaGATA17和McaGATA18基因在低浓度盐胁迫下能显著增强酵母细胞的耐盐性，但在高浓度盐胁迫下，McaGATA18基因的耐盐效果最为突出。

关键词: 金铃子, GATA基因家族, 盐胁迫, 酵母