关键词: 旱地土壤；微生物； , DNA提取, 提取方法改良

Abstract: In order to explore a rapid, simple, economical and efficient DNA extraction method of microorganisms in dryland soil,based on the extraction method of DNA from paddy soil by Qin et al,an improved experiment was carried out:(1)placing 0.3 g dry soil in 300 μL aseptic water for overnight at -80℃ ;(2)determining ratio of CTAB to SDS with the total volume of 500 μL,and exploring the effect of adding glass bead or not,finally the microbial DNA extraction effect of modified method was studied compared with the reagent box method.The results showed that when placing 0.3 g dry soil in 300 μL aseptic water for overnight at -80℃ ,and adjusting the volume ratio of CTAB to SDS to be 100∶400,the highest content of microbial DNA extracted from red soil in dryland by the improved method was 6.29 μg/g,which was lower than the reagent box method(7.46 μg/g),the purity was better than the reagent box method,and the PCR amplification effect was good.Compared with the reagent box method,the improved method was more economical and efficient.Therefore,the modified method was suitable for the extraction of microbial DNA from red soil in dryland.

Key words: Dryland soil, Microbe, DNA extraction, Improvement of extraction method