猪流行性腹泻病毒基因组和诊断方法研究进展

doi:10.15933/j.cnki.1004-3268.2024.10.002

摘要/Abstract

摘要： 猪流行性腹泻病毒（Porcine epidemic diarrhea virus，PEDV）引起的猪流行性腹泻（Porcine epidemic diarrhea，PED），是一种急性、高度接触性、高发病率和高致死率的猪肠道传染病，给全球养猪业造成了巨大的经济损失。深入了解PEDV的基因组结构和功能是研制高效安全疫苗的关键。通过解析PEDV的基因组结构、编码的蛋白质以及其与宿主细胞的相互作用，研究人员可以设计出更具针对性的疫苗候选物。诊断技术的进步对于PED的早期诊断及免疫后应答水平监测和评估至关重要。因此，综述了PEDV的基因组结构与功能（包括S、E、M、N、辅助蛋白和非结构蛋白）、血清学以及分子诊断方法［如酶联免疫吸附试验（ELISA）、聚合酶链式反应（PCR）、环介导等温扩增（LAMP）、病毒中和（VN）和间接免疫荧光测定（IFA）、免疫层析试验（ICA）、荧光微球免疫测定（FMIA）、序列测定和聚类规则间隔短回文重复序列（CRISPR）-CRISPR相关蛋白（Cas）］的最新进展，旨在为建立快速有效的PEDV检测方法提供思路，并为临床上有效防控PED提供参考。

关键词: 猪流行性腹泻病毒, 猪流行性腹泻, 基因组, 诊断

Abstract: Porcine epidemic diarrhea(PED)caused by porcine epidemic diarrhea virus(PEDV)is an acute,highly contagious,enteric disease of pigs with high morbidity and mortality,thereby bringing huge economic losses to the pig industry all over the world.A better understanding of PEDV’s genome and function is key to developing an effective and safe vaccine. By understanding PEDV’s genomic structure,the proteins it encodes,and its interactions with host cells,researchers can design more targeted vaccine candidates.Advances in PED diagnostic technology are essential for early diagnosis and monitoring and evaluation of post⁃immune response.In this review,we summarized the latest progress of PEDV genome structure and function including S,E,M,N,accessory proteins and non⁃structural proteins,serology and molecular diagnostic methods,such as enzyme⁃linked immunosorbent assay,polymerase chain reaction,ring⁃mediated isothermal amplification,viral neutralization and indirect immunofluorescence assays,immunochromatographic tests,fluorescent microsphere immunoassays,sequence assays and clustered regularly inter spaced short palindromic repeats(CRISPR)⁃Cas,in order to provide ideas for establishing rapid and effective PEDV detection methods,and provide reference for effective prevention and control of PED in practice.

Key words: PEDV, PED, Genome, Diagnosis

中图分类号:

S858.28

张玉杨. 猪流行性腹泻病毒基因组和诊断方法研究进展[J]. 河南农业科学, 2024, 53(10): 12-20.

ZHANG Yuyang. Research Progress in Genome and Diagnostic Methods of Porcine Epidemic Diarrhea Virus[J]. Journal of Henan Agricultural Sciences, 2024, 53(10): 12-20.

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