马铃薯块茎蛾取食胁迫下马铃薯根际土壤微生物代谢功能多样性变化

doi:10.15933/j.cnki.1004-3268.2023.11.012

摘要/Abstract

摘要： 为研究昆虫取食胁迫对植物根际土壤微生物群落功能多样性的影响，采用Biolog ECO技术研究了马铃薯块茎蛾取食胁迫对云南省2个马铃薯主栽品种（合作88、丽薯6号）根际土壤微生物群落多样性以及土壤碳源利用的影响，以未受虫害胁迫为空白对照。结果显示，马铃薯块茎蛾取食胁迫提高了衡量根系微生物代谢活性的平均颜色变化率（AWCD，96 h），即取食胁迫处理下2个马铃薯品种根际土壤微生物对31种碳源利用的AWCD值均提高，且在取食胁迫第7天达到最大；同时，取食胁迫处理下2个马铃薯品种根际微生物的多样性Shannon和Simpson指数均高于对照，且在取食胁迫第7 天达到最高；对其碳源利用的分析表明，在取食胁迫下，2个马铃薯品种的根际微生物对6类碳源的利用率不同，但2个品种胁迫处理均提高了根际微生物对各类碳源的利用；另外，主成分分析表明，取食胁迫处理改变了2个马铃薯品种根系微生物的群落结构。综合以上结果表明，马铃薯块茎蛾取食胁迫能提高马铃薯根际土壤微生物群落的功能多样性。

关键词: 马铃薯, 马铃薯块茎蛾, 根际微生物, 代谢功能, 取食胁迫

Abstract: In order to study the effects of insect feeding stress on the functional diversity of plant rhizosphere soil microbial community，the effects of potato tuber moth，Phthorimaea operculella Zeller feeding stress on the rhizosphere soil microbial community diversity and soil carbon source utilization of two major potato varieties（Hezuo 88 and Lishu No. 6）in Yunnan Province were tested by Biolog ECO technology，with no pest feeding stress as control.The results showed that potato tuber moth feeding stress increased the average color change rate（AWCD）（96 h），which measures the metabolic activity of root microorganisms，and the AWCD values of using 31 carbon sources by rhizosphere soil microorganisms in two feeding stressed potato varieties all increased，and reached the maximum on the 7th day of feeding stress.At the same time，the diversity indices of Shannon and Simpson of the two feeding stress treatments were higher than those of the control groups，and reached the maximum on the 7th day after feeding stress.The analysis of carbon source utilization showed that under feeding stress，the utilization of various carbon sources was improved by the rhizosphere microorganisms in both two stressed groups although their utilization rates were different.In addition，principal component analysis showed that，the feeding stress changed the community structure of root microorganisms of the two varieties.The above results showed that feeding stress of potato tuber moth increased the functional diversity of potato rhizosphere soil microbial community.

Key words: Potato, Phthorimaea operculella Zeller, Rhizosphere soil microorganisms, Metabolic function, Feeding stress

中图分类号:

S435.32

唐芬芬, 杨伟克, 谢昆, 张祖芸, 李娜. 马铃薯块茎蛾取食胁迫下马铃薯根际土壤微生物代谢功能多样性变化[J]. 河南农业科学, 2023, 52(11): 104-112.

TANG Fenfen, YANG Weike, XIE Kun, ZHANG Zuyun, LI Na. Changes of Metabolic Functional Diversity of Potato Rhizosphere Soil Microorganisms under Feeding Stress of Potato Tuber Moth，Phthorimaea operculella Zeller[J]. Journal of Henan Agricultural Sciences, 2023, 52(11): 104-112.

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