河南农业科学 ›› 2021, Vol. 50 ›› Issue (5): 137-141.DOI: 10.15933/j.cnki.1004-3268.2021.05.019

• • 上一篇    下一篇

硒都黑猪OLR1基因多态性及其与产仔数的相关性分析

乔木,周佳伟,吴俊静,武华玉,梅书棋,彭先文   

  1. (湖北省农业科学院 畜牧兽医研究所/动物胚胎工程及分子育种湖北省重点实验室,湖北 武汉 430064)
  • 收稿日期:2020-11-12 出版日期:2021-05-15 发布日期:2021-05-15
  • 通讯作者: 彭先文(1974-),男,湖北恩施人,研究员,博士,主要从事猪遗传育种研究。E-mail:pxwpal@163.com
  • 作者简介:乔木(1982-),女,辽宁建平人,副研究员,博士,主要从事猪遗传育种研究。E-mail:mqbetter@163.com
  • 基金资助:
    湖北省农业科学院人才项目(Q2018017);湖北省农业科技创新中心资助项目(2019-620-000-001-18);湖北省自然科学基金项目(2018CFA014);湖北省重大创新专项(2019ABA084);湖北省农业科技创新行动项目(2018skjcx05)

Polymorphism of OLR1 Gene and Its Association with Litter Size in Xidu Black Pigs

QIAO Mu,ZHOU Jiawei,WU Junjing,WU Huayu,MEI Shuqi,PENG Xianwen   

  1. (Institute of Animal and Veterinary Science,Hubei Academy of Agricultural sciences/Huibei Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Wuhan 430064,China)
  • Received:2020-11-12 Published:2021-05-15 Online:2021-05-15

摘要: 为研究OLR1基因遗传多态性对硒都黑猪产仔数的影响,利用PCR扩增和直接测序法检测OLR1基因在硒都黑猪中的单核苷酸多态性(SNP),并与母猪第一胎的产仔数性状进行相关性分析。结果显示,在基因的g.61808357处发现3种类型的碱基突变A>C、A>T和C>T,存在AA、AC、CC、AT和TC 5种基因型,AC、AA和CC基因型个体的总产仔数和产活仔数均极显著(P<0.01)高于TC和AT基因型个体。综上,OLR1基因g.61808357处SNP位点可作为影响硒都黑猪产仔数性状的分子标记。

关键词: 硒都黑猪, OLR1基因, 多态性, 产仔数, 关联分析, 分子标记

Abstract: In order to explore the effect of OLR1 gene genetic polymorphism on litter size of Xidu black pigs,the single nucleotide polymorphism (SNP) of OLR1 gene in Xidu black pigs was detected by PCR amplification and direct sequencing,and the association analysis with litter size of first parity sows was carried out.The results showed that there were three mutations (A>C,A>T and C>T) at g.61808357,and there were five genotypes AA,AC,CC,AT and TC.The total number born and number born alive of individuals with AC,AA and CC genotypes were extremely significantly higher than those with TC and AT genotypes (P<0.01).In conclusion,the SNP at g.61808357 of OLR1 gene can be used as a molecular marker affecting litter size trait of Xidu black pigs.

Key words: Xidu black pig, OLR1 gene, Polymorphism, Litter size, Association analysis;Molecular marker

中图分类号: