关键词: 绵羊, GDF9 基因, 多态性, 产羔数, 生物信息学分析

Abstract: In order to explore the genetic effect of GDF9 gene coding region polymorphism and reproductive performance in sheep，five sheep populations of Dorper sheep，hybrid of Tan and Han sheep，hybrid F1 generation，hybrid F2 generation and cross H1 generation were used to detect four missense mutations in GDF9 gene by Sequenom Mass ARRAY® SNP technique，and their association with litter size was analyzed. The results showed that the genotypes of rs425223128，rs597009987 and rs415699275 loci were all homozygous，and there were CC，TC and TT genotypes at rs160076408 locus in five populations. The association analysis between different genotypes of rs160076408 locus and litter size of five populations showed that there was a certain correlation between rs160076408 locus and litter size in hybrid F1 generation population，in which the litter size of TC genotype was significantly higher than that of CC genotype（P<0.05）. It could be seen that the rs160076408 locus was suitable for the breeding of multi⁃lamb trait in the hybrid F1 generation of sheep. Bioinformatics analysis results showed that the theoretical isoelectric point of sheep GDF9 protein was 9.08，the instability coefficient was 54.43，the fat coefficient was 77.31，and the total average hydrophilicity was -0.404. The secondary structure of GDF9 protein changed before and after rs160076408 locus mutation. Protein interaction network analysis showed that GDF9 protein interacted with proteins related to animal reproduction.

Key words: Sheep, GDF9 gene, Polymorphism, Lambing number, Bioinformatics