关键词: 烤烟, 次生代谢, 有机酸, 生物碱, 多酚, 甾醇, 河南烟区

Abstract: In order to explore the differences in the content of main secondary metabolites of flue-cured tobacco leaves in different producing areas in Henan,the contents of organic acids,alkaloids,polyphenols and sterols in the tobacco leaves of the C3F and B2F grades of the main flue-cured tobacco varieties in seven different producing areas in Henan were determined by GC/MS-SIM,GC-MS,HPLC and UPLCMS,and the test results were analyzed. Through descriptive statistical analysis,malic acid,citric acid and oxalic acid were the main components of flue-cured tobacco organic acids; nicotine, nornicotine,pseudoequiine and neonicotine were the main components of flue-cured tobacco alkaloids;chlorogenic acid and rutin were the main components of flue-cured tobacco polyphenols;stigmasterol and β-sitosterol were the main components of flue-cured tobacco sterols. The organic acid content of flue-cured tobacco leaves in Henan tobacco area was 120.01—216.01 mg/ g,with an average of 151.77 mg/ g;the alkaloid content was 18.85—29.97 mg/ g, with an average of 23.13 mg/g; the content of polyphenols was 16.85—27.92 mg/ g,and the average was 21.74 mg/ g;the sterol content was 2.11—2.87 mg/ g,and the average was 2.54 mg/g.There were differences in the total amount of secondary metabolites in tobacco leaves from different producing areas,which showed Fangcheng>Luoning>Biyang>Lushi>Jiaxian>Xiangxian> Linying.Fangcheng flue-cured tobacco had the highest organic acid and alkaloid content;Xiangxian and Jiaxian flue-cured tobacco had the highest polyphenol content;Luoning flue-cured tobacco had the highest sterol content.

Key words: Flue-cured tobacco, Secondary metabolism, Organic acid, Alkaloid, Polyphenol, Sterol;Henan tobacco district