河南农业科学 ›› 2021, Vol. 50 ›› Issue (3): 25-32.DOI: 10.15933/j.cnki.1004-3268.2021.03.004

• 作物栽培·遗传育种 • 上一篇    下一篇

籼稻品种Y两优886高氮素利用效率机制研究

祁雪姣,谢乾坤,谢雨欣,梁晓娅,李俊周,孙虎威   

  1. (河南农业大学 河南粮食作物协同创新中心/河南省水稻生物学重点实验室,河南 郑州 450046)
  • 收稿日期:2020-10-13 出版日期:2021-03-15 发布日期:2021-03-15
  • 通讯作者: 孙虎威(1986-),男,河南郑州人,副教授,博士,主要从事植物营养学生理及分子方向。 E-mail:sunhuwei19431@163.com
  • 作者简介:祁雪姣(1998-),女,河南安阳人,在读硕士研究生,研究方向:水稻氮素高效利用。E-mail:qixuejiao1218@163.com
  • 基金资助:
    国家自然科学基金项目(31601821)

Mechanism of High Nitrogen Utilization Efficiency of Indica Rice Yliangyou 886

QI Xuejiao,XIE Qiankun,XIE Yuxin,LIANG Xiaoya,LI Junzhou,SUN Huwei   

  1. (Collaborative Innovation Center of Henan Grain Crops/Key Laboratory of Rice Biology in Henan Province,Henan Agricultural University,Zhengzhou 450046,China)
  • Received:2020-10-13 Published:2021-03-15 Online:2021-03-15

摘要: 为了研究籼稻Y两优886高氮素利用效率的机制,采用水培和土培试验,在2个氮浓度(正常供氮和低氮)下测定粳稻品种日本晴和籼稻品种Y两优886的生物量、总氮含量、根系发育情况、根系一氧化氮水平以及氮转运基因的表达情况。结果表明,在正常供氮和低氮条件下,Y两优886的生物量、总氮含量、根系一氧化氮水平均显著高于日本晴;但侧根密度没有发生显著变化。实时荧光定量PCR结果表明,与日本晴相比,在正常供氮和低氮条件下,Y两优886根系中多个氮转运基因表达量均显著上调。综上,Y两优886根系具有较强的合成一氧化氮的能力,从而促进氮转运相关基因的表达,进而提高对氮的利用效率。

关键词: 籼稻, 氮素利用效率, 根系, 氮, 一氧化氮

Abstract: To determine the mechanism of high nitrogen utilization efficiency of indica rice Yliangyou 886,the biomass, total nitrogen ( N) content, root development, nitric oxide(NO) levels of root and expression levels of N transport genes in root were analyzed in Nipponbare (japonica) and Y liangyou 886(indica) under two N concentrations(normal N concentration and low N concentration) by hydroponics and soil culture experiments.The results showed that the biomass,total N content and nitric oxide level of root were increased significantly in Yliangyou 886 compared with those in Nipponbare under the two N concentrations.However,the lateral root density of the two cultivars did not change significantly under two N concentrations. qRT-PCR results showed that the expression levels of several N transport genes in the roots were significantly up-regulated in Yliangyou 886 compared with Nipponbare under two N concentrations.To sum up,root of Yliangyou 886 has a strong ability to synthesize nitric oxide,thereby promoting the expression of several N transport genes and improving the N use efficiency.

Key words: Indica rice, Nitrogen use efficiency, Root, Nitrogen, Nitric oxide

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