硫化氢对大豆铝胁迫耐受性的增强作用

doi:10.15933/j.cnki.1004-3268.2019.10.010

摘要/Abstract

摘要： 铝毒害是酸性土壤地区制约农作物生产的主要逆境因子。为明确作物抵抗铝毒害的机制，以大豆为试验材料，研究了新型植物气体信号分子硫化氢（H₂S）在铝胁迫耐受性中的调节作用。结果显示，铝胁迫处理诱导大豆根尖H₂S的产生。添加外源H₂S供体处理显著缓解了铝胁迫诱导的根伸长受抑，以及根尖铝含量、根尖细胞死亡率、根尖丙二醛和活性氧（H₂O₂和O₂·）含量升高，而添加H₂S合成抑制剂处理则产生了相反的效应，加重了铝胁迫对大豆的伤害；进一步研究显示，铝胁迫下添加H₂S供体处理增强了根尖抗氧化酶（APX、CAT、POD、SOD）的活性，而添加H₂S合成抑制剂处理则抑制了抗氧化酶活性的增强。表明铝胁迫下H₂S通过提高根尖抗氧化能力来清除活性氧的过量积累，缓解铝胁迫对根伸长的抑制。

关键词: 大豆, 硫化氢, 耐铝性, 活性氧, 抗氧化酶

Abstract: Aluminium (Al) toxicity is a major constraint on agricultural production in acidic soils.To elucidate how crops cope with Al toxicity，the role of hydrogen sulphide (H₂S) ,a novel gas signaling molecule in plants,was investigated in soybean under Al stress.Ｒesults showed that Al treatment induced H₂S production in root tips.Furthermore,application of an H₂S donor significantly alleviated Al-induced inhibition of root elongation,and decreased Al accumulation,cell deathrate,malondialdehyde and reactive oxygen species ( H₂O₂and O₂) contents in root tips,whereas the treatment with an H₂S inhibitor elicited the opposite effects.Further investigation showed that H2 S donor increased the activities of antioxidant enzymes (APX,CAT,POD and SOD),while the H₂S inhibitor treatment inhibited the antioxidant enzymes activities in root tips under Al stress.These results indicate that H₂S alleviates Al induced root elongation inhibition through enhancement of antioxidant defenses in soybean,subsequently reducing reactive oxygen species accumulation.

Key words: Soybean, Hydrogen sulphide, Aluminum tolerance, Ｒeactive oxygen species, Antioxidant en zymes

中图分类号:

S565.1

刘文文, 纪芳, 王华华. 硫化氢对大豆铝胁迫耐受性的增强作用[J]. 河南农业科学, 2019, 48(10): 64-69.

LIU Wenwen, JI Fang, WANG Huahua. The Ｒole of Hydrogen Sulphide in Improving Aluminium Tolerance of Soybean[J]. Journal of Henan Agricultural Sciences, 2019, 48(10): 64-69.

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