河南农业科学 ›› 2020, Vol. 49 ›› Issue (1): 61-67.DOI: 10.15933/j.cnki.1004-3268.2020.01.008

• 作物栽培·遗传育种 • 上一篇    下一篇

大豆耐低磷性状鉴定及优异种质筛选

武海燕,李喜焕,李文龙,孔佑宾,杜汇,张彩英   

  1. (河北农业大学/教育部华北作物种质资源研究与利用重点实验室,河北 保定 071001)
  • 收稿日期:2019-07-28 出版日期:2020-01-15 发布日期:2020-01-15
  • 通讯作者: 杜 汇(1982-),女,河北新乐人,副教授,博士,主要从事大豆遗传育种研究。E-mail:duhui668@126.com 张彩英(1960-),女,河北深泽人,研究员,博士,主要从事大豆遗传育种与转基因研究。E-mail:zhangcaiying@hebau.edu.cn
  • 作者简介:武海燕(1986-),女,河北邯郸人,在读硕士研究生,研究方向:大豆分子育种。E-mail:wuhaiyan_hyw@163.com
  • 基金资助:
    河北农业大学引进留学博士专项(ZD201602);现代农业科技奖励性后补助资金项目(17927670H);河北省科技计划项目(16227516D-1)

Identification of Low Phosphorus Tolerant Traits and Selection of Elite Genotypes in Soybean

WU Haiyan,LI Xihuan,LI Wenlong,KONG Youbin,DU Hui,ZHANG Caiying   

  1. (Hebei Agricultural University/North China Key Laboratory of Crop Germplasm Resources,Education Ministry of China,Baoding 071001,China)
  • Received:2019-07-28 Published:2020-01-15 Online:2020-01-15

摘要: 为发掘耐低磷大豆新种质,培育耐低磷新品种,以前期构建的含有247个株系的大豆耐低磷遗传群体为材料,在低磷和适磷处理下分别测定各株系的株高、根长、根鲜质量、根干质量、地上部鲜质量、地上部干质量、根冠比、根系磷含量和根系磷利用率等9个耐低磷相关性状,并评价供试株系的耐低磷特性,筛选耐低磷优异种质。结果表明,9个参试性状中,除株高外,其余8个性状在不同磷处理间、供试材料间均存在显著或极显著差异,说明低磷处理有效且各株系对低磷逆境的适应性不同。主成分分析将上述8个存在显著差异的单一性状转化为2个综合指标,根据隶属函数和株系耐低磷综合评价值,结合聚类分析将供试247个株系分为耐低磷、中间型和不耐低磷3种类型,明确了各株系的耐低磷特性,并筛选出ZN-251、ZN-299、ZN-229等14个耐低磷优异种质。

关键词: 大豆, 耐低磷遗传群体, 低磷胁迫, 主成分分析, 聚类分析, 优异种质

Abstract: In order to explore new germplasms and develop new varieties of soybean with low phosphorus(P) tolerance,a low phosphorus tolerant genetic population with 247 lines was used in this study.Nine traits related to low P tolerance including plant height,root length,fresh weight of root,dry weight of root,fresh weight of shoot,dry weight of shoot,root/ shoot ratio,phosphorus content and utilization efficiency of root were studied under different P treatment conditions. The characteristics of the tested lines resistance to low phosphorus were evaluated and elite germplasms were screened. The results showed that among the nine tested traits,except for plant height,there were(extremely) significant differences between different P treatments and between different materials,indicating that the low phosphorus treatment was effective and the adaptabilities of lines were different.Then these eight characteristics were transformed into two comprehensive indexes through the principal component analysis method,and the comprehensive evaluation values(D value) of 274 lines were calculated.Finally,the 247 lines were divided into 3 groups,low P tolerant-,intermediate- and sensitive-group. In addition,14 elite germplasms including ZN-251,ZN-299 and ZN-229 were selected for their high tolerance to low P stress.

Key words: Soybean, genetic population tolerant to low phosphorus, Low phosphorus stress, Principal component analysis, Cluster analysis, Elite germplasms

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