河南农业科学 ›› 2023, Vol. 52 ›› Issue (4): 21-30.DOI: 10.15933/j.cnki.1004-3268.2023.04.003

• 作物栽培·遗传育种 • 上一篇    下一篇

不同小麦品种(系)耐低磷能力综合评价与代表性评价指标筛选

陈泽东1,师焕婷1,董殿琪1,王亚鑫1,王金凤1,虞波1,王鹏飞1,康国章1,2   

  1. (1.河南农业大学国家小麦工程技术研究中心,河南 郑州 450046;2.神农种业实验室,河南 郑州 450002)
  • 收稿日期:2023-02-04 出版日期:2023-04-15 发布日期:2023-05-15
  • 通讯作者: 康国章(1971-),男,河南伊川人,研究员,博士,主要从事小麦遗传育种研究。E-mail:guozhangkang@henau.edu.cn。王鹏飞同为通信作者
  • 作者简介:陈泽东(1996-),男,河南信阳人,在读硕士研究生,研究方向:小麦磷高效分子育种。E-mail:zedongchen2020@163.com
  • 基金资助:
    神农种业实验室项目(SN01-2022-01);河南省重点研发与推广专项(科技攻关项目)(222102110039)

Comprehensive Evaluation of Low Phosphorus Tolerance of Different Wheat Varieties(Lines)and Screening of Representative Evaluation Indexes

CHEN Zedong1,SHI Huanting1,DONG Dianqi1,WANG Yaxin1,WANG Jinfeng1,YU Bo1,WANG Pengfei1,KANG Guozhang1,2   

  1. (1.National Engineering Research Centre for Wheat,Henan Agricultural University,Zhengzhou 450046,China;2.The Shennong Laboratory,Zhengzhou 450002,China)
  • Received:2023-02-04 Published:2023-04-15 Online:2023-05-15

摘要: 以11个黄淮麦区小麦品种(系)为材料,通过水培和池栽土培试验,对水培小麦苗期的株高、单株总根长、生物量、各组织磷含量等15个性状指标以及池栽小麦成熟期的株高、地上部分干质量、单株籽粒产量等9个性状指标的耐低磷系数进行分析,利用主成分分析和隶属函数法等综合评价不同小麦品种(系)的耐低磷能力并进行聚类分析,利用灰色关联度分析各性状指标的耐低磷系数与耐低磷综合评价值间的关联度以筛选耐低磷代表性评价指标,为小麦耐低磷研究及磷高效小麦新品种的选育提供筛选依据和种质资源。结果表明,苗期小麦部分指标的耐低磷系数间存在相关关系,说明评价小麦耐低磷的指标发生信息重叠;主成分分析将苗期的15个指标转化为4个独立的综合指标;对综合指标进行隶属函数值计算,进而得到耐低磷综合评价值,发现不同品种(系)苗期的耐低磷能力表现为新麦67>矮抗58>百农607>豫农908>济麦22>周麦18>鑫华麦818>天麦189>丰德存麦5号>安农188>安农203。同样地,主成分分析将小麦成熟期的9个指标转化为3个独立的综合指标,并得到成熟期的耐低磷综合评价值,发现不同品种(系)成熟期的耐低磷能力表现为新麦67>济麦22>安农203>鑫华麦818>矮抗58>安农188>丰德存麦5号>豫农908>周麦18>百农607>天麦189。结合各品种(系)在苗期和成熟期的综合表现,通过聚类分析将11个小麦品种(系)划分为3类,其中新麦67、济麦22和矮抗58为耐低磷型品种(系),豫农908、鑫华麦818、百农607和周麦18为较耐低磷型品种,丰德存麦5号、安农188、安农203和天麦189为低磷敏感型品种(系)。通过灰色关联分析,发现苗期小麦根磷含量、根磷积累量、单株总根长和成熟期磷收获指数、秸秆干质量、地上部分干质量这6个指标的耐低磷系数与小麦的耐低磷综合评价值联系密切,可将上述指标作为小麦耐低磷能力评价的关键指标。

关键词: 小麦, 耐低磷系数, 指标筛选, 隶属函数法, 综合评价

Abstract: Eleven wheat varieties(lines)from the Huang⁃Huai wheat region were treated with normal and phosphorus deficiency in hydroponic and pond soil culture experiments.The low phosphorus tolerance coefficients of 15 indicators(plant height,total root length per plant,biomass and phosphorus concentration in various tissues,etc.)at seedling stage and 9 indicators(plant height,aboveground dry weight,grain yield per plant,etc.)at maturity stage were analyzed. Further,the principal component analysis and membership function method were used to comprehensively evaluate the low phosphorus tolerance of wheat varieties(lines),and cluster analysis was conducted;the gray correlation degree analysis was used to analyze the correlation strength between the low phosphorus tolerance coefficient and the comprehensive evaluation value of low phosphorus tolerance for screening representative evaluation indicators for low phosphorus tolerance,so as to provide screening basis and germplasm resources for the study of wheat tolerance to low phosphorus and the breeding of wheat varieties with high phosphorus efficiency.The results showed that there was correlation among the low phosphorus tolerance coefficients of indicators at seedling stage,indicating that there was information overlap in the indicators used to evaluate wheat low phosphorus tolerance.Principal component analysis transformed 15 indicators at seedling stage into 4 independent comprehensive indicators.By calculating the membership function value of the comprehensive indicators,the comprehensive evaluation value of low phosphoru tolerance was obtained.The low phosphorus tolerance order of varieties(lines)at seedling stage was Xinmai 67>Aikang 58>Bainong 607>Yunong 908>Jimai 22>Zhoumai 18>Xinhuamai 818>Tianmai 189>Fengdecunmai 5>Annong 188>Annong 203. Similarly,principal component analysis transformed 9 indicators of wheat at maturity stage into 3 independent comprehensive indicators,and obtained the comprehensive evaluation value of low phosphorus tolerance at maturity stage.The low phosphorus tolerance order of wheat varieties(lines)at maturity was Xinmai 67>Jimai 22>Annong 203>Xinhuamai 818>Aikang 58>Annong 188>Fengde cunmai 5>Yunong 908>Zhoumai 18>Bainong 607>Tianmai 189.Combined the comprehensive performance of each variety(line)at seedling stage and maturity stage,by cluster analysis,the 11 wheat varieties(lines)were divided into three types:low phosphorus tolerant(Xinmai 67,Jimai 22 and Aikang 58),slightly low phosphorus tolerant(Yunong 908,Xinhuamai 818,Bainong 607 and Zhoumai 18)and low phosphorus sensitive(Fengdecunmai 5,Annong 188,Annong 203 and Tianmai 189).Through gray correlation analysis,the low phosphorus tolerance coefficient of phosphorus concentration and accumulation in root,total root length per plant at seedling stage,and the phosphorus harvest index,dry weight of straw and dry aboveground weight at maturity stage were closely related to the comprehensive evaluation value of low phosphorus tolerance,indicating that these six indicators could be regarded as the representative evaluation indexes of wheat low phosphorus tolerance.

Key words: Triticum aestivum L., Low phosphorus tolerance coefficient, Indicators screening, Membership function method, Comprehensive evaluation

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