关键词: 石榴, ZF-HD, 生物信息学, 启动子分析, 基因复制事件

Abstract: The gene family of ZF⁃HD was identified through a genome⁃wide study of Punica granatum to gain a deeper understanding of its function. Based on the systematic bioinformatics analyses，fifteen PgZF-HD genes were identified，the number of encoded amino acids ranged from 98 to 365，PgZF⁃HD03 and PgZF⁃HD04 were acidic proteins，and other family members were basic proteins，all family members were unstable hydrophilic proteins，more than 70% PgZF⁃HD proteins were located in the nuclear.In the secondary structure，the proportion of beta⁃turn was the lowest，and that of random coil was the highest.
Fifteen PgZF‑HD genes were classified into five categories by phylogenetic tree analysis，namely ZF⁃HDⅠ，ZF⁃HD Ⅱ ，ZF⁃HD Ⅲ ，ZF⁃HD Ⅴ and MIF. The ZF⁃HD Ⅲ category contained the most members of PgZF‑HD，no PgZF‑HD gene was classified in the ZF⁃HDⅣ category. It was found that both motif2 and motif4 were highly conserved and contained multiple highly conserved active sites.The largest amount of PgZFmotif was found in ZF⁃HDⅡ，and motif8 was identified only in ZF⁃HDⅠ.Most PgZF‑HD genes had only one exon，the gene structure was simple，and the same class of genes had similar conservative motif and gene structure.Chromosome mapping and gene duplication analysis showed that 15 PgZF‑HD genes were inhomogeneously distributed on eight chromosomes，three gene pairs were identified to have large fragment duplication events and no tandem duplication events occurred.Promoter analysis showed that the PgZF‑HD genes played an important role in plant hormone response，growth and development，and stress response.

Key words: Punica granatum, ZF?HD, Bioinformatics, Promoter analysis, Gene duplication event