关键词: 烟草, 脂氢过氧化物裂解酶（HPL）, 基因克隆, 生物信息学, 抗旱

Abstract: In order to explore the function of lipid hydroperoxidelyase(HPL)in tobacco,NtHPL gene was cloned from leaves,and the transgenic tobacco plants with overexpression of NtHPL were obtained to research the stress resistance function of NtHPL. NtHPL gene contained an intron and two exons,and the full-length of its cDNA was 1 494 bp.The C-terminal of NtHPL protein included the conservative sequence of the cytochrome P450 protein family,and N-terminal contained a chloroplast transit peptide.The expression of NtHPL could be found in tobacco roots,stems and leaves,and its expression was the highest in leaves. Mechanical damage,methyl jasmonate(MeJA),salicylic acid(SA),ethylene,abscisic acid(ABA) and drought stress could increase the expression level of NtHPL in leaves. Under drought stress,proline content was significantly higher in the transgenic tobacco plants with overexpression of NtHPL than that in the wild type K326,and malondialdehyde(MDA)content,peroxidase(POD) and superoxide dismutase(SOD) activities were lower than those in the wild type K326.These results showed that the NtHPL could improve the drought-resistant ability of tobacco plants,and it could be used for the breeding of tobacco drought-resistant varieties.

Key words: Tobacco, Hydroperoxidelyase (HPL), Gene cloning, Bioinformatics, Drought resistance