关键词: 白菜, NPF基因家族, 生物信息学, 理化性质, 保守基序

Abstract: The NPF gene family is a nitrate transporter ubiquitous in plants，and is also responsible for transporting glucosinolates，peptides，potassium salts，and plant hormones. In order to clarify the function and evolutionary relationship of the NPF gene family of Chinese cabbage，bioinformatics methods were used to identify the members of the NPF gene family of Chinese cabbage，and the physical and chemical properties，secondary structure，subcellular location，conserved motif distribution and evolutionary relationship with Arabidopsis were analyzed for the NPF gene family proteins.The gene structure and chromosome location of the NPF gene family members of Chinese cabbage were also analyzed.The results showed that there were 72 members in the NPF gene family of Chinese cabbage，the number of encoded amino acids ranged from 497 to 1 117，the molecular weight ranged from 54. 49 to 123. 21 ku，the isoelectric point ranged from 5.72 to 9.47，and most of them were stable basic proteins. The secondary structure was dominated by alpha helix，followed by random coils. Subcellular localization prediction showed that they were all on the plasma membrane. The phylogenetic tree divided Chinese cabbage NPF gene family proteins into 8 sub⁃families，which were highly conserved. Most of the NPF gene family members in Chinese cabbage contained 4 CDS sequences.The 72 NPF gene family members were unevenly distributed on 10 chromosomes of Chinese cabbage.

Key words: Chinese cabbage, NPF gene family, Bioinformatics, Physical and chemical properties, Conservative motif