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    Screening of Kidney Bean Varieties for Safe Production in Cadmium Polluted Farmland of Northwest Guizhou
    CHAI Guanqun, ZHOU Lixing, QIN Song, FAN Chengwu, LIU Guihua, WANG Li, JIANG Ya
    Journal of Henan Agricultural Sciences    2023, 52 (6): 51-60.   DOI: 10.15933/j.cnki.1004-3268.2023.06.006
    Abstract (1950)      PDF (1385KB)(772)       Save
    In order to explore the differences in the absorption and enrichment of cadmium(Cd) by different kidney bean varieties and the effects of varieties and environment on them,and to screen out the Cd low⁃enriched kidney bean varieties suitable for planting in northwestern Guizhou,13 local kidney bean varieties were used as materials,and in the Cd⁃contaminated farmland of L site and Y site in northwestern Guizhou,a field plot experiment was conducted.The results showed that the variety,the environment and interaction between variety and environment had extremely significant effects on yield and Cd content of kidney bean,respectively. There was significant difference in Cd content of different kidney bean varieties in the same place. The maximum value of Cd(in Hongpiqiuzijiadou) and the minimum value of Cd(in Bangda No.2) could be 10 times different in the same place.The bioconcentration factor(BCFCd)of Cd in soils for different kidney bean varieties in L site and Y site were 0.06%—0.0% and 0.13%—0.93%,respectively.The Cd content of 13 tested kidney bean varieties showed the safe level.The yield of Bangda No.2 was the highest among the 13 varieties,reaching 18 513.6 and 19 287.9 kg/ha in L and Y sites,respectively.In summary,13 kidney bean varieties can be used as recommended varieties for Cd⁃contaminated areas in northwestern Guizhou,and Bangda No.2 was preferred because of its high yield and low Cd content.

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    Growth Response and Tolerance Evaluation of 32 Spinach Varieties to Cadmium Stress
    MIAO Mingjun, LI Jiajia, LEI Xiaokui, LIN Yang, LIAO Jichao, ZHONG Jian, LI Ju, LI Yuejian, CHANG Wei
    Journal of Henan Agricultural Sciences    2024, 53 (9): 108-116.   DOI: 10.15933/j.cnki.1004-3268.2024.09.011
    Abstract (1890)      PDF (2257KB)(342)       Save
    Cadmium(Cd)is a highly toxic heavy metal element.To identify Cd‑tolerant spinach varieties and screen related identification indexes,and provide a theoretical basis for the selection and breeding of Cd‑tolerant varieties,in the present study,we conducted a hydroponic experiment using 32 spinach varieties in China.The spinach was exposed to Cd stress(Cd concentration of 50 μmol/L)for 20 days,while the other group of plants was treated as a control(Cd concentration of 0 μmol/L).After the plants reached maturity,we measured the agronomic traits and Cd content.The integrated Cd tolerance of each spinach variety was evaluated by principal component analysis,affiliation function analysis,and correlation analysis.The results showed that plant height,root length,underground fresh weight,aboveground fresh weight,aboveground Cd content,underground Cd content and translocation coefficient of the participating varieties under Cd stress conditions were significantly different from the control.Under Cd stress,the growth and biomass accumulation of spinach were inhibited,and the indicators of each trait decreased significantly.The order of average reduction of each agronomic index was aboveground fresh weight>plant height>underground fresh weight>root length.The principal component analysis of Cd stress tolerance index of the seven indexes was performed,and three principal components were selected,with a cumulative variance contribution of 81.552%;The composite evaluation value(D value)showed highly significant positive correlations(P<0.01)with plant height,underground fresh weight,aboveground fresh weight,aboveground Cd content,and translocation coefficient,which can be used as the preferred indexes for the evaluation of Cd tolerance in spinach.Based on the principal component analysis,we classified the 32 spinach varieties into three categories:Cd‑tolerant,moderate,and Cd‑sensitive varieties.Among the varieties tested,Jin’ge and Micro orchid exhibited strong Cd tolerance,while Desai 176 was found to be Cd‑sensitive.

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    Effects of Simultaneous Biochar and Nitrogen Fertilizer Application on Uptake and Accumulation of Cd by Pepper in Lime Soil
    CHAI Guanqun, LIU Guihua, LUO Muxinjian, WANG Li, QIN Song, FAN Chengwu
    Journal of Henan Agricultural Sciences    2022, 51 (9): 68-78.   DOI: 10.15933/j.cnki.1004-3268.2022.09.007
    Abstract (1770)      PDF (1564KB)(1973)       Save
    In order to explore the effect of combined application of biochar and nitrogen fertilizer on the uptake and accumulation of cadmium(Cd)by pepper in calcareous soil,and to provide scientific basis for the development of special carbon based fertilizer for pepper,pot experiment was conducted to study the effects of four amounts of biochar(180,270,360,450 mg/kg,expressed as B1,B1.5,B2,B2.5)combined with 180 mg/kg nitrogen fertilizer of three forms(amide nitrogen,ammonium nitrogen,nitrate nitrogen,expressed as UN,AN and NN)on pepper fruit yield,rhizosphere soil pH and available Cd content,as well as pepper’s absorption and accumulation of Cd.The results showed that nitrogen fertilizer form had a very significant effect on pH value and available Cd content of rhizosphere soil,the pH value of rhizosphere soil treated with UNB2 was the highest and the available Cd content was the lowest.The dry weight of fruit,leaf,stem and root in NNB2.5 treatment was the highest,and the dry weight of fruit was 37. 68 g/pot.The fruit dry weight of UNB2 treatment was 34.65 g/pot,which was not significantly different from that of NNB2.5 treatment.The content of Cd in fruit,stem and leaf of UNB2 treatment was the lowest,and the content of Cd in fruit was 0.032 mg/kg,which was lower than the limit standard(<0.05 mg/kg)in National Food Safety Standard for Contaminants in Foods(GB 2762—2017).The transfer coefficient of Cd from different parts to fruit was TFfruit/stem>TFfruit/leaf>TFfruit/root.The correlation analysis of Cd content in fruit showed that Cd content in pepper fruit mainly came from the redistribution of shoot vegetative organs (stem and leaf).The cumulative distribution of Cd in root of pepper was the largest.In conclusion,UNB2 was preferentially recommended as the formula of special carbon⁃based fertilizer for pepper to achieve the safe production of pepper in calcareous soil with Cd.

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    Effects of Silicon and Selenium on Photosynthesis and Antioxidant Enzyme System of Cucumber Seedlings under Cadmium Stress
    YU Minmin, FAN Wenhua, LIU Fenwu, TIAN Ludan, WANG Gailing, MENG Qinghui
    Journal of Henan Agricultural Sciences    2023, 52 (1): 116-124.   DOI: 10.15933/j.cnki.1004-3268.2023.01.012
    Abstract (1692)      PDF (1494KB)(784)       Save
    To explore the effects of silicon(Si)and selenium(Se)on photosynthesis and antioxidant enzyme system of cucumber seedlings under cadmium(Cd) stress in calcareous soil,and provide a theoretical basis for the application of Si and Se in alleviating Cd toxicity,pot experiment was conducted to study the effects of single or simultaneous application of Si(300 mg/kg)and Se(2,4 mg/kg)on cucumber seedling growth,chlorophyll content,photosynthetic parameters and antioxidant enzyme activity under Cd(5 mg/kg)stress. The results showed that Cd stress had obvious toxic effects on plant growth,leaf photosynthesis and antioxidant enzyme activities in cucumber seedlings. Compared with blank control,the chlorophyll content,net photosynthetic rate(Pn),transpiration rate(Tr) and stomatal conductance(Gs)of cucumber leaves under Cd treatment were decreased significantly,the intercellular carbon dioxide concentration(Ci)increased significantly,the activities of peroxidase(POD),superoxide dismutase(SOD) and catalase(CAT) decreased significantly,while the content of malongizldehyde(MDA)increased significantly. Cd toxicity was alleviated under the application of 300 mg/kg Si or 2,4 mg/kg Se,which could promote the growth of cucumber,increase the chlorophyll content and Pn,Tr,Gs and POD,SOD,CAT activities of cucumber leaves,and reduce the Ci and MDA content. The effect of simultaneous application of Si and Se was more obvious than that of single application of Si or Se,and the application of 300 mg/kg Si and 2 mg/kg Se had the best effect. Compared with single application of Cd,Pn,Tr and Gs of cucumber leaves significantly increased by 18.53%,42.67% and 87.50%,respectively,Ci significantly decreased by 35.61%,POD,SOD and CAT activities significantly increased by 87.65%,32.40% and 23.58%,respectively,and MDA content significantly decreased by 42. 68%.The results show that applying Si and Se in calcareous soil can alleviate the toxicity of Cd to cucumber,enhance the photosynthesis of cucumber leaves and improve the activity of antioxidant enzyme system,and the simultaneous application of Si and Se has a certain synergistic effect.

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    Effects of Exogenous Nitric Oxide on Seed Germination,Seedling Growth and Antioxidant Characteristics of Lettuce under Cadmium Stress
    XIONG Mingguo
    Journal of Henan Agricultural Sciences    2021, 50 (10): 109-115.   DOI: 10.15933/j.cnki.1004-3268.2021.10.014
    Abstract (1671)      PDF (2769KB)(380)       Save
    In order to explore the mitigation effects of exogenous NO on seed germination and seedling growth of lettuce under cadmium(Cd)stress,the effects of exogenous sodium nitroprusside(SNP,NO donor)with different concentrations(100,150,200,250 μmol/L)on seed germination,seedling growth and antioxidant properties of lettuce under 100 μmol/L Cd stress were studied. The results showed that exogenous NO had no significant effect on lettuce seed germination rate and germination potential under Cd stress,but generally significantly improved lettuce seedling growth,chlorophyll content and activities of peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD),and significantly decreased malondialdehyde(MDA)content. The treatment with 250 μmol/L SNP had the best effect under Cd stress,and POD,CAT,SOD activities and chlorophyll content significantly increased by 85.00%,81.20%,63.27% and 155.63% respectively. Overall,exogenous NO could mitigate the damage of lettuce caused by Cd,and 250 μmol/L SNP had the best effect.
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    Effects of Foliar Spraying of Na2SiO3 on the Yield,Quality and Cd Uptake of Pod Pepper
    CHAI Guanqun, SUI Yanfeng, YANG Fan, QIN Song, FAN Chengwu
    Journal of Henan Agricultural Sciences    2023, 52 (7): 109-116.   DOI: 10.15933/j.cnki.1004-3268.2023.07.011
    Abstract (1473)      PDF (1319KB)(237)       Save
    The effects of foliar spraying of 0,3,5 mmol/L(CK,Si3,Si5)Na2SiO3 solution on the yield,quality and Cd absorption and transport capacity of Capsicum annuum L.under 0.3 mg/L Cd stress were investigated by hydroponic experiment,in order to provide scientific basis for the development of foliar inhibitors.The results showed that with the increase of the dosage of Na2SiO3,the biomass of all parts of pod pepper cultivar‘Qing Hong Yuan Shuai’increased. Compared with CK,Si5treatment significantly increased the biomass of all parts,the fresh and dry weight of fruits increased by 16.29% and 15.26%,respectively,and the dry weight of whole plant increased by 21.35%.With the increase of the dosage of Na2SiO3,the contents of soluble sugar,soluble protein,amino acid,vitamin C(Vc)and capsaicin showed increasing trend,and Si5 treatment significantly improved the quality.The order of Cd content in different parts of each treatment was as follows:leaves on main stems>roots>leaves on fork stems>fruits>main stems>fork stems. Compared with CK,foliar spraying of Na2SiO3 solution promoted the decrease of Cd content in all parts of pepper,and the decrease extents of Cd content in fruits with Si3 and Si5 were 11.30%,28.69%,respectively.The difference of Cd accumulation in the whole plant of each treatment was not significant. With the increase of Na2SiO3 concentration,the Cd accumulation in fruits decreased significantly,and the Cd accumulation in stems,leaves and roots increased significantly.Compared with CK,the Cd accumulation in fruits of Si5 treatment decreased by 19.43%. Compared with CK,foliar application of Na2SiO3 solution significantly reduced the transfer efficiency of Cd from roots,fork stems,main stems and leaves of main stems,stem and leaf to fruits(TFfruit/root,TFfruit/fork stem,TFfruit/main stem,TFfruit/leaf of main stem,TFfruit/stem and leaf).Spraying Na2SiO3solution on the leaves increased the accumulation ability of Cd in the stems,leaves and roots of pod pepper cultivar‘Qing Hong Yuan Shuai’,and limited the transport of Cd from vegetative organs(stems,leaves and roots)to fruits.

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    Effects of Phytohormones and Chelating Agents on the Development and Remediation Effect of Portulaca oleracea L.under Cadmium Stress
    GUO Menglu, LI Jianqi, LU Yu
    Journal of Henan Agricultural Sciences    2023, 52 (9): 102-109.   DOI: 10.15933/j.cnki.1004-3268.2023.09.010
    Abstract (1115)      PDF (1458KB)(324)       Save
    To study the effects of phytohormones and chelating agent EDTA combined treatment on the development and repair effect of Portulaca oleracea L.under cadmium(Cd)stress,three phytohormone and EDTA combined treatments were set under cadmium stress(FI:20 mg/L Cd+1.5 mg/L EDTA+100 mg/L IAA;FG:20 mg/L Cd+1.5 mg/L EDTA+500 mg/L GA;FS:20 mg/L Cd+1.5 mg/L EDTA+100 mg/L SA),with distilled water as blank control(CK),and 20 mg/L Cd treatment as Cd stress control(Cd),and seeds of Portulaca oleracea L.were soaked in different liquids.The seed germination potential,germination rate,seedling height,taproot length,fresh weight,as well as the accumulation and transport coefficient of Cd in various parts of the seedlings were measured.The results showed that compared with Cd stress control,the seed germination potential and germination rate of the three composite treatments were significantly increased.Among them,the FG treatment had the largest increase in germination potential and germination rate,with an increases of 19.24% and 25.31%,respectively.The seedling height,taproot length,and fresh weight all significantly increased,among them the FG treatment had the largest increase in seedling height and fresh weight,with increases of 47.95% and 35.91% compared with Cd stress control,respectively.The accumulation and translocation factor of Cd in the roots and shoots of Portulaca oleracea L.seedlings significantly increased.Among them,the accumulation and translocation factor of Cd in the shoots and roots of Portulaca oleracea L.seedlings treated with FG were the highest,2.31,2.94,and 2.33 times of those treated with Cd stress control,respectively.In summary,the combined treatment of phytohormones and EDTA can increase the germination potential and percentage of Portulaca oleracea L.seeds under Cd stress,increase the seedling height,taproot length,fresh weight,and increase the accumulation and translocation factor of Cd.Among them,the combined treatment of GA and EDTA has the best effect.

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    Effects of Activated Phosphate Rock Powder on Transformation of Cadmium Fraction in Soil and Cadmium Uptake by Pepper
    CHAI Guanqun, YANG Jiaojiao, WANG Li, LIU Guihua, LUO Muxinjian, QIN Song, FAN Chengwu
    Journal of Henan Agricultural Sciences    2023, 52 (5): 91-98.   DOI: 10.15933/j.cnki.1004-3268.2023.05.011
    Abstract (1008)      PDF (1345KB)(211)       Save
    In order to explore the effect of activated phosphate rock powder on the transformation of cadmium(Cd)fraction in Cd‐safely‐utilized lime soil and the absorption of Cd by pepper,and to provide scientific basis for the development and utilization of phosphate rock powder,the Cd‐safely‐utilized lime soil and pepper variety‘Qing Hong Yuan Shuai’were selected as test materials. The effects of no phosphate rock powder(CK),0.5% phosphate rock powder(PR‐0.5%),0.5% oxalic acid activated phosphate rock powder(CPR‐0.5%)and 0.5% citric acid activated phosphate rock powder(NPR‐0. 5%)on soil Cd fraction,pepper biomass and fruit Cd content were studied by indoor culture experiment and pot experiment.The results showed that compared with the control(CK),the dry weight of pepper fruits treated with unactivated and activated phosphate rock powder increased significantly by 13.99%—41.79%,and the dry weight of pepper fruits treated with NPR‐0.5% was the largest.With the prolongation of culture time,the content of available Cd in soil decreased. Compared with 0 d,after 90 d of soil culture,the decrease of available Cd in CK,PR‐0.5%,CPR‐0.5% and NPR‐0.5% was 26.67%,26.67%,31.11% and 35.56%,respectively.The application of unactivated and activated phosphate rock powder promoted the transformation of activated Cd fraction in soil to inert fraction,and the content of activated Cd fraction in NPR‐0.5% treatment was the lowest. Compared with CK,the proportion of activated Cd fraction in NPR‐0.5% treatment decreased by 16.45 percentage points.Compared with CK,the Cd content of pepper fruits treated with unactivated and activated phosphate rock powder decreased by 14.04%—31.58%,and the Cd content of pepper fruits treated with NPR‐0.5% decreased the most.In summary,NPR‐0.5% treatment promoted the transformation of activated Cd fraction in soil to inert fraction and inhibited the absorption of Cd by pepper.

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