Effects of Red and Blue LED Light Cycles on thePhysiological⁃biochemistry and Nutrient Content of Dendrobium officinale Test⁃Tube Seedlings

doi:10.15933/j.cnki.1004-3268.2026.07.008

Abstract

Abstract: To investigate the effects of different LED red（R）and blue（B）light cycles on the growth and development，photosynthesis，and nutrient accumulation of Dendrobium officinale tissue culture seedlings，tissue culture seedlings of D.officinale were used as experimental material.Based on the previously screened optimal LED red and blue combination light（R∶B=5∶5），five different light cycle treatments were set up［ white light control group（CK），treatment group A（3 h/d），treatment group B（6 h/d），treatment group C（9 h/d） and treatment group D（12 h/d）］.After 120 days of illumination，growth parameters（plant height，number of leaves，leaf length，stem diameter，fresh weight，dry weight），antioxidant parameters［superoxide dismutase（SOD） activity，malondialdehyde（MDA）content，ascorbate peroxidase（APX）activity，peroxidase（POD） activity，catalase（CAT）activity］，photosynthetic pigment parameters（chlorophyll a content，chlorophyll b content，chlorophyll a+b content，chlorophyll a/b，and carotenoid content），chlorophyll fluorescence parameters［PSⅡ maximum photochemical efficiency（Fv/Fm），PSⅡ potential activity（Fv/Fo），actual quantum efficiency（Φ_PSⅡ），electron transport rate（ETR Ⅱ），photochemical quenching coefficient（qP），non⁃photochemical quenching coefficient（qN）］ and nutrient content（amino acids，alkaloids，polysaccharides，flavonoids） were determined.The results showed that the plant height，stem diameter，fresh weight and dry weight of the seedlings reached the peak in treatment D，while the leaf length and number of leaves reached the peak in treatment C. APX，CAT and POD activities peaked in treatment B，SOD activity peaked in treatment C，and the MDA content was the lowest in treatment C. Overall，the nutrient content，photosynthetic pigment indicators and chlorophyll fluorescence parameters of the seedlings were the best in treatment B.There were significant or extremely significant correlations between growth indicators，antioxidant indicators，nutrient indicators and chlorophyll fluorescence parameters and photosynthetic pigment indicators.The principal component analysis results showed that the comprehensive performance of Dendrobium officinale in treatment B was the best in terms of growth indicators，antioxidant indicators，photosynthetic indicators and nutrient accumulation，with a comprehensive score of 3.35.In conclusion，when the red and blue light ratio is 5∶5，LED light exposure for 6 h has the best promoting effect on the growth and development and nutrient accumulation of Dendrobium officinale.

Key words: Dendrobium officinale, LED photoperiod, Physiology and biochemistry, Nutrient, Chlorophyll fluorescence

摘要： 为探究不同LED红（R）蓝（B）光周期对铁皮石斛试管苗生长发育、光合作用及营养成分积累的影响，以铁皮石斛试管苗为材料，以前期筛选出的最优LED红蓝组合光（R∶B=5∶5）为基础，设置5种不同的光周期处理［白光对照组（CK）、处理组 A（3 h/d）、处理组B（6 h/d）、处理组C（9 h/d）和处理组D（12 h/d）］，光照120 d后测定生长指标（株高、叶片数、叶长、茎粗、鲜质量、干质量）、抗氧化指标［超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、抗坏血酸过氧化物酶（APX）活性、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性］、光合色素指标（叶绿素a含量、叶绿素b含量、叶绿素a+b含量和叶绿素a/b及类胡萝卜素含量）、叶绿素荧光指标［PSⅡ最大光化学效率（Fv/Fm）、PSⅡ潜在活性（Fv/Fo）、实际光量子效率（Φ_PSⅡ）、电子传递速率（ETRⅡ）、光化学淬灭系数（qP）、非光化学淬灭系数（qN）］及营养成分（氨基酸、生物碱、多糖、类黄酮）含量。结果表明，试管苗株高、茎粗、鲜质量和干质量在D处理达到峰值，叶长和叶片数在C处理达到峰值。APX、CAT和POD活性在B处理达到峰值，SOD活性在C处理时达到峰值，MDA含量在C处理时最低。试管苗营养物质和光合色素指标及叶绿素荧光参数等指标整体上以B处理最优。生长指标、抗氧化指标、营养指标与叶绿素荧光参数和光合色素指标间存在显著或极显著相关关系。主成分分析结果显示，B处理铁皮石斛在生长指标、抗氧化指标、光合指标及营养物质成分积累等方面综合表现最优，综合得分达3.35。综上，采用红蓝光比为5∶5、LED光照射6 h/d，促进铁皮石斛生长发育及营养成分积累的效果最好。

关键词: 铁皮石斛, LED光周期, 生理生化, 营养物质, 叶绿素荧光

CLC Number:

S567.239

Zheng Lifang, Wang Jiangning, Meng Xinya, Fu Qiunuan, Wang Yuxin, Lu Jiajing. Effects of Red and Blue LED Light Cycles on thePhysiological⁃biochemistry and Nutrient Content of Dendrobium officinale Test⁃Tube Seedlings[J]. Journal of Henan Agricultural Sciences, 2026, 55(7): 70-81.

郑礼方, 王江宁, 孟新亚, 符秋暖, 王宇鑫, 陆嘉婧. LED红蓝光周期对铁皮石斛试管苗生理生化及营养物质含量的影响[J]. 河南农业科学, 2026, 55(7): 70-81.

Figures/Tables 9

References

［1］许亚楠，闫家榕，孙鑫，等. 红光和远红光在调控植物生长发育及应答非生物胁迫中的作用［J］. 植物学报，2023，58（4）：622⁃637.
Xu Y N，Yan J R，Sun X，et al. Red and far⁃red light regulation of plant growth，development，and abiotic stress responses［J］. Bulletin of Botany，2023，58（4）：622⁃637.

［2］Zhang N，Liu Y J，Lu Y L，et al. Transcriptomic and metabolomic analyses reveal the molecular mechanisms by which long⁃day photoperiods promote flowering in Gossypium hirsutum L.［J］. Frontiers in Plant Science，2025，16：1657595.

［3］Chen S M，Shan B，Zheng F H，et al. Influence of photoperiod on endogenous phytohormone levels and cadmium⁃related gene expression in Sedum alfredii［J］.Plant Signaling & Behavior，2025，20（1）：2544317.

［4］谢析颖. 霍山石斛生物钟相关基因克隆及其与培养物糖含量分析［D］. 福州：福建农林大学，2017.

Xie X Y. Cloning of circadian clock⁃related genes in Dendrobium huoshanense and their relations with polysaccharides content［D］. Fuzhou：Fujian Agriculture and Forestry University，2017.

［5］石勇志. 不同光质及光周期对花椰菜幼苗生长的影响［D］. 泰安：山东农业大学，2022.

Shi Y Z. Effects of different light quality and photoperiod on growth of cauliflower seedlings［D］.Taian：Shandong Agricultural University，2022.

［6］张月昌. 不同光质及光周期对芫荽生长的影响［D］. 泰安：山东农业大学，2023.

Zhang Y C. Effects of different light quality and photoperiod on growth of coriander［D］. Taian：Shandong Agricultural University，2023.

［7］陈冰星，王晓倩，刘涛，等. 不同光质光周期对樱桃萝卜生长发育及营养品质的影响［J］. 西北植物学报，2020，40（1）：77⁃86.

Chen B X，Wang X Q，Liu T，et al. Effect of different light quality and photoperiods on growth development and nutritional quality of cherry radish［J］. Acta Botanica Boreali⁃Occidentalia Sinica，2020，40（1）：77⁃86.

［8］赵慧，李利平，王璐璐，等. 不同光质和光周期对樱桃番茄幼苗生长及果实品质的影响［J］. 江苏农业学报，2025，41（8）：1592⁃1605.

Zhao H，Li L P，Wang L L，et al. Effects of different light qualities and photoperiods on the growth of cherry tomato seedlings and fruit quality［J］. Jiangsu Journal of Agricultural Sciences，2025，41（8）：1592⁃1605.

［9］谢慈江，何福英，刘莉，等. 光质和光周期对山白兰苗木生长、生理的影响［J］. 广西植物，2023，43（12）：2362⁃2373.

Xie C J，He F Y，Liu L，et al. Effects of light quality and photoperiod on growth and physiology of Michelia baillonii seedlings［J］. Guihaia，2023，43（12）：2362⁃2373.

［10］吴芳兰，李书玲，杨梅，等. LED光质及光周期对香子含笑幼苗生长和光合特性的影响［J］. 广西植物，2022，42（12）：2167⁃2177.

Wu F L，Li S L，Yang M，et al. Effects of LED light qualities and photoperiods on growth and photosynthetic characteristics of Michelia gioii［J］.Guihaia，2022，42（12）：2167⁃2177.

［11］Xu L，Zeng X X，Liu Y N，et al. Effect of Dendrobium officinale polysaccharides on central nervous system disease：based on gut microbiota［J］. International Journal of Biological Macromolecules，2023，240：124440.

［12］王治丹，代云飞，罗尚娟，等. 铁皮石斛化学成分及药理作用的研究进展［J］. 华西药学杂志，2022，37（4）：472⁃476.
Wang Z D，Dai Y F，Luo S J，et al. Research progress on chemical constituents and pharmacological effects of Dendrobium officinale［J］. West China Journal of Pharmaceutical Sciences，2022，37（4）：472⁃476.

［13］Jiao C Y，Wei M K，Fan H H，et al. Transcriptomic analysis of genes related to alkaloid biosynthesis and the regulation mechanism under precursor and methyl jasmonate treatment in Dendrobium officinale［J］.Frontiers in Plant Science，2022，13：941231.

［14］林丹霓，黄君婷，钟玉鸣，等. 兜唇石斛发酵多肽抗氧化活性与氨基酸结构的关系［J］. 现代食品科技，2023，39（10）：139⁃150.
Lin D N，Huang J T，Zhong Y M，et al. Relationship between the oxidative activity and amino acid structure of fermented polypeptides from Dendrobium aphyllum［J］. Modern Food Science & Technology，2023，39 （10）：139⁃150.

［15］Fan C Y，MANIVANNAN A，Wei H. Light quality⁃mediated influence of morphogenesis in micropropagated horticultural crops：a comprehensive overview［J］. BioMed Research International，2022，2022（1）：4615079.

［16］Morańska E，Simlat M，Warchoł M，et al. Phenolic acids and Amaryllidaceae alkaloids profiles in Leucojum aestivum L. in vitro plants grown under different light conditions［J］. Molecules，2023，28（4）：1525.

［17］于鹏澎，宋金修，蔡玮，等. 夜间LED补光光照度和补光时间对番茄种苗质量的影响［J］. 江苏农业学报，2023，39（9）：1917⁃1926.
Yu P P，Song J X，Cai W，et al. Effects of LED supplementary light intensity and supplementary light time at night on seedling quality of tomato in the controlled environment［J］. Jiangsu Journal of Agricultural Sciences，2023，39（9）：1917⁃1926.

［18］林琼瑜，郑礼方，孟新亚，等. LED光质对铁皮石斛试管苗生理生化及营养物质含量的影响［J］. 河南农业科学，2025，54（2）：66⁃76.
Lin Q Y，Zheng L F，Meng X Y，et al. Effects of LED light quality on physiology，biochemistry and nutrient content of Dendrobium officinale test⁃tube seedlings［J］. Journal of Henan Agricultural Sciences，2025，54（2）：66⁃76.

［19］李合生. 植物生理生化实验原理和技术［M］. 北京：高等教育出版社，2000：36⁃43.
Li H S. Principles and techniques of plant physiological and biochemical experiments［M］.Beijing：Higher Education Press，2000：36⁃43.

［20］Nakano Y，Asada K. Hydrogen peroxide is scavenged by ascorbate⁃specific peroxidase in spinach chloroplasts［J］. Plant and Cell Physiology，1981，22（5）：867⁃880.

［21］Aebi H. Catalase in vitro［J］ Methods in Enzymology，1984，5（1）：121⁃126.

［22］陈建勋，王晓峰. 植物生理学实验指导［M］. 2版. 广州：华南理工大学出版社，2006：56⁃78.
Chen J X，Wang X F. Guidance for plant physiology experiments［M］. 2nd ed. Guangzhou：South China University of Technology Press，2006：56⁃78.

［23］王学奎. 植物生理生化实验原理和技术［M］. 2版. 北京：高等教育出版社，2006：89⁃93.
Wang X K. Principles and techniques of plant physiological and biochemical experiments［M］. 2nd ed.Beijing：Education Press，2006：89⁃93.

［24］Lichtenthaler H K，Buschmann C. Chlorophylls and carotenoids： measurement and characterization by UV⁃VIS spectroscopy［J］. Current Protocols in Food Analytical Chemistry，2001，1（1）：F4. 3. 1⁃F4. 3. 8.

［25］陈永快，王涛，兰婕，等. 植物工厂内LED光调控在作物栽培中的研究进展［J］. 江苏农业科学，2020，48（23）：40⁃46.
Chen Y K，Wang T，Lan J，et al. Research progress of LED light regulation in plant factories in crop cultivation［J］. Jiangsu Agricultural Sciences，2020，48 （23）：40⁃46.

［26］殷丛培，石昭瑾，田成，等. 红蓝光对植物光合碳同化和生长发育的影响研究进展［J］. 应用生态学报，2025，36（7）：2246⁃2256.
Yin C P，Shi Z J，Tian C，et al. Effects of red and blue light on photosynthetic carbon assimilation and growth⁃development in plants：a review［J］. Chinese Journal of Applied Ecology，2025，36（7）：2246⁃2256.

［27］陈文昊，徐志刚，刘晓英，等. LED光源对不同品种生菜生长和品质的影响［J］. 西北植物学报，2011，31（7）：1434⁃1440.
Chen W H，Xu Z G，Liu X Y，et al. Effect of LED light source on the growth and quality of different lettuce varieties［J］. Acta Botanica Boreali⁃Occidentalia Sinica，2011，31（7）：1434⁃1440.

［28］毛金柱，邱权，张芳，等. LED光源下不同光照时间对生菜生长的影响［J］. 农机化研究，2014，36（3）：141⁃145.
Mao J Z，Qiu Q，Zhang F，et al. Effects of different light durations on the growth of lettuce（Lactuca sativa L.）in LED light source［J］. Journal of Agricultural Mechanization Research，2014，36（3）：141⁃145.

［29］何蔚. 不同光周期和光质配比对番茄和辣椒苗生长的影响［D］. 杨凌：西北农林科技大学，2017.
He W. Effects of different photoperiod and light quality ratio on the growth of tomato and pepper seedlings［D］. Yangling：Northwest A & F University，2017.

［30］王智勇，唐贝贝，王久兴. 不同光照时间对叶用莴苣产量及品质的影响［J］. 农业工程技术，2021，41（25）：66⁃69.
Wang Z Y，Tang B B，Wang J X. Effects of different illumination time on yield and quality of leaf lettuce［J］.Agricultural Engineering Technology，2021，41（25）：66⁃69.

［31］Ahmadi T，Shabani L，Sabzalian M R. LED light mediates phenolic accumulation and enhances antioxidant activity in Melissa officinalis L. under drought stress condition［J］. Protoplasma，2020，257 （4）：1231⁃1242.

［32］Mushtaq N U， Saleem S，Rasool A，et al. Antioxidant defense in plants［M/OL］. Springer Cham，2022.https：//link. springer. com/chapter/10. 1007/978-981-16-7981⁃0_2.

［33］Li S C. Novel insight into functions of ascorbate peroxidase in higher plants：more than a simple antioxidant enzyme［J］. Redox Biology，2023，64：102789.

［34］赵明伟，吕新，张泽，等. 不同红蓝LED光照时间对冰菜生长和品质的影响［J］. 新疆农业科学，2021，58（1）：80⁃91.
Zhao M W，Lü X，Zhang Z，et al. Effects of different red and blue LED lighting time on the growth and quality of ice plan［t J］. Xinjiang Agricultural Sciences，2021，58（1）：80⁃91.

［35］张伟，孟祥庆，苏晓荟，等. 光调控对药用植物次生代谢成分合成的影响［J］. 药学实践与服务，2024，42（2）：50⁃59.
Zhang W，Meng X Q，Su X H，et al. Effects of light regulation on the synthesis of secondary metabolites in medicinal plants［J］. Journal of Pharmaceutical and Service，2024，42（2）：50⁃59.

［36］林小苹，赖钟雄. 不同光质对铁皮石斛原球茎增殖及有效成分含量的影响［J］. 热带作物学报，2015，36（10）：1796⁃1801.
Lin X P，Lai Z X. Effect of light quality on the proliferation of protocorm and active ingredient contents of Dendrobium officinale［J］. Chinese Journal of Tropical Crops，2015，36（10）：1796⁃1801.

［37］彭金彬. 光质对金钗石斛生长及品质的影响［J］. 福建林业科技，2015，42（4）：67⁃71.
Peng J B. Effects of different light quality on the growth and effective components of Dendrobium nobile［J］. Journal of Fujian Forestry Science and Technology，2015，42（4）：67⁃71.

［38］梁祎，郝文琴，石玉，等. 不同光周期下叶面喷施纳米硒对生菜生长和品质的影响［J］. 中国生态农业学报（中英文），2022，30（1）：82⁃91.
Liang Y，Hao W Q，Shi Y，et al. Effects of nano⁃Se foliar spraying and photoperiod on lettuce growth and quality［J］. Chinese Journal of Eco⁃Agriculture，2022，30（1）：82⁃91.

［39］蔡葛平. 光周期、土壤水分及外源激素对黄芩中黄酮类成分累积的影响及其分子机制［D］. 上海：复旦大学，2008.
Cai G P. Impacts and mechanisms of photoperiod，soil water availability and external hormones on the accumulation of flavonoids in Scutellaria baicalensis Georgi［D］. Shanghai：Fudan University，2008.

［40］Li X B，Yang K G，Ma J，et al. Transcriptome analysis reveals different red and blue light duration promote growth and main medicinal ingredients of Dendrobium nobile Lindl［J］. Industrial Crops and Products，2023，204：117347.

［41］邢阿宝，崔海峰，俞晓平，等. 光质及光周期对植物生长发育的影响［J］. 北方园艺，2018（3）：163⁃172.
Xing A B，Cui H F，Yu X P，et al. Effects of different lights qualities and photoperiods on plant growth and development［J］. Northern Horticulture，2018（3）：163⁃172.
［42］杨梦平. 不同光质LED光源对马铃薯组培苗光合色素含量的影响［J］. 黑龙江农业科学，2022（6）：27⁃30.

Yang M P. Effects of LED light source with different light quality on photosynthetic pigment content of potato tissue culture seedlings［J］. Heilongjiang Agricultural Sciences，2022（6）：27⁃30.

［43］刘杰，胡笑涛，王文娥，等. 光强和光周期对水培生菜光合及叶绿素荧光特性的影响［J］. 西南农业学报，2019，32（8）：1784⁃1790.
Liu J，Hu X T，Wang W E，et al. Effects of light intensity and photoperiod on photosynthetic characteristics and chlorophyll fluorescence of hydroponic lettuce［J］. Southwest China Journal of Agricultural Sciences，2019，32（8）：1784⁃1790.

［44］杨浞露，王芳. 光周期对马铃薯产量与光合作用的影响［J］. 江苏农业科学，2021，49（10）：64⁃71.
Yang Z L，Wang F. Impacts of photoperiod on potato yield and photosynthesis［J］. Jiangsu Agricultural Sciences，2021，49（10）：64⁃71.

［45］鲍顺淑，贺冬仙，郭顺星. 可控环境下光照时间对铁皮石斛组培苗生长发育的影响［J］. 中国农业科技导报，2007，9（6）：90⁃94.
Bao S S，He D X，Guo S X. Suitable photoperiod of Dendrobium officinale in vitro under controlled environment［J］. Journal of Agricultural Science and Technology，2007，9（6）：90⁃94.

［46］李紫琴，王家强. 浅谈叶绿素荧光技术在作物长势监测中的应用［J］. 农业与技术，2023，43（5）：7⁃9.
Li Z Q，Wang J Q. Application of chlorophyll fluorescence technology in crop growth monitoring［J］.Agriculture and Technology，2023，43（5）：7⁃9.

［47］邹泉，邢伟明，王若玎，等. 根施丛枝菌根真菌和独脚金内酯对盐碱胁迫下多年生黑麦草叶绿素荧光及抗氧化系统的影响［J］. 河南农业科学，2025，54（6）：72⁃83.
Zou Q，Xing W M，Wang R D，et al. Effects of root application of AMF and SL on chlorophyll fluorescence and antioxidant system of Lolium perenne under saline alkali stress［J］. Journal of Henan Agricultural Sciences，2025，54（6）： 72⁃83.

［48］梁亚男，邬梦晞，肖雍琴，等. 植物根际促生菌和褪黑素对大花金鸡菊抗氧化特性和砷吸收的影响［J］. 河南农业科学，2025，54（3）：87⁃98.
Liang Y N，Wu M X，Xiao Y Q，et al. Effect of PGPR and melatonin on the antioxidant properties and arsenic absorption of Coreopsis grandiflora［J］. Journal of Henan Agricultural Sciences，2025，54（3）： 87⁃98.

［49］余德炤，江晓东，杨莹颖，等. 基于RGB与高光谱图像的小麦低温胁迫评估模型［J］. 南方农业学报，2025，56（1）：97⁃110.
Yu D Z，Jiang X D，Yang Y Y，et al. Assessment model for low temperature stress in wheat based on RGB and hyperspectral images［J］. Journal of Southern Agriculture，2025，56（1）： 97⁃110.

［50］李圆圆，张文静，李楠. 砷胁迫下接种PGPR对水生植物砷累积及生理特性的影响［J］. 河南农业科学，2024，53（6）：67⁃78.
Li Y Y，Zhang W J，Li N. Effects of PGPR on arsenicaccumulation and physiological characteristics of aquatic plants under arsenic stress［J］. Journal of Henan Agricultural Sciences，2024，53（6）： 67⁃78.

［51］齐学礼，王永霞，陈鹏，等. 不同胁迫下郑麦0943的耐低氮高温特性研究［J］. 河南农业大学学报，2024，58（2）：187⁃194.
Qi X L，Wang Y X，Chen P，et al. Study on physiological characters of a wheat variety named Zhengmai 0943 to low nitrogen and high temperature stresses［J］. Journal of Henan Agricultural University，2024，58（2）：187⁃194.

［52］郭嘉，熊定伟，杨丽娟，等. 高温胁迫下2，4-表油菜素内酯和丛枝菌根真菌对四季杜鹃光合特性及激素代谢的影响［J］. 河南农业科学，2025，54（10）：110⁃120.
Guo J，Xiong D W，Yang L J，et al. Effects of 2，4⁃epibrassinolide and arbuscular mycorrhizal fungi on photosynthetic characteristics and hormone metabolism of Rhododendron hybridum under high temperature stress［J］. Journal of Henan Agricultural Sciences，2025，54（10）： 110⁃120.

［53］陈孝丑，杨芮，户帅雅，等. 红蓝复合光对‘阳光红心’红掌生长和叶绿素荧光反应的影响［J］. 热带作物学报，2018，39（2）：224⁃230.
Chen X C，Yang R，Hu S Y，et al. Effects of blue and red light⁃emitting diodeon t he growth and chlorophyll fluorescence reaction of Anthurium andraeanum ‘Yongonred’［J］. Chinese Journal of Tropical Crops，2018，39（2）：224⁃230.

［54］樊小雪，杨亚娜，徐刚. 不同光质处理对樱桃番茄生长发育和叶绿素荧光的影响［J］. 福建农业学报，2019，34（9）：1026⁃1031.
Fan X X，Yang Y N，Xu G. Effects of LED light on growth and chlorophyll fluorescence of cherry tomatoes［J］. Fujian Journal of Agricultural Sciences，2019，34（9）：1026⁃1031.
［55］Wang Y，Tong Y F，Chu H L，et al. Effects of different light qualities on seedling growth and chlorophyll fluorescence parameters of Dendrobium officinale［J］.Biologia，2017，72（7）：735⁃744.
［56］张舵，刘有春，魏鑫，等. pH值对蓝莓生长及生理影响的机制解析［J］. 江苏农业科学，2025，53（3）：148⁃156.

Zhang D，Liu Y C，Wei X，et al. Mechanism analysis of effects of pH value on growth and physiology of blueberry［J］. Jiangsu Agricultural Sciences，2025，53（3）：148⁃156.
［57］杨振超，何蔚，牟孙涛，等. 不同光周期和红蓝光质配比对辣椒幼苗生长发育的影响［J］. 农业工程学报，2017，33（17）：173⁃180.

Yang Z C，He W，Mu S T，et al. Plant growth and development of pepper seedlings under different photoperiods and photon flux ratios of red and blue LEDs［J］. Transactions of the Chinese Society of Agricultural Engineering，2017，33（17）：173⁃180.
［58］罗媛，叶林，郭文忠，等. 红蓝光补光对设施番茄生长发育及荧光特性的影响［J］. 中国农业气象，2024，45（5）：506⁃516.
Luo Y，Ye L，Guo W Z，et al. Influence by supplementary red and blue light on the growth and development of greenhouse tomatoes and fluorescence characteristics［J］. Chinese Journal of Agrometeorology，2024，45（5）：506⁃516.

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