Effects of LED Light Quality on Physiology，Biochemistry and Nutrient Content of Dendrobium officinale Test⁃tube Seedlings

doi:10.15933/j.cnki.1004-3268.2025.02.008

Abstract

Abstract: To investigate the impact of different LED light spectrums on the growth，photosynthesis，and nutrient accumulation of Dendrobium officinale test⁃tube seedlings，using D.officinale test⁃tube seedlings as the experimental material，six different light quality treatments were established［100% red light（R），100% blue light（B），80% red light+20% blue light（8R∶2B），70% red light+30% blue light（7R∶3B），and 50% red light+50% blue light（5R∶5B），white fluorescent light used as a control group（CK）］.The growth indexes（plant height，leaf number，maximum leaf length，stem diameter，fresh weight，dry weight），antioxidant indexes［superoxide dismutase（SOD） activity，malondialdehyde（MDA） content，ascorbate peroxidase（APX）activity，peroxidase（POD）activity，catalase（CAT）activity）］，photosynthetic indexes（chlorophyll a content，chlorophyll b content，chlorophyll a+b content，chlorophyll a/b ratio，carotenoids content），chlorophyll fluorescence indexes（Fv/Fm，Φ_PSⅡ，ETRⅡ，qN，qP，Fv/Fo）and nutrient contents（amino acids，alkaloids，polysaccharides）were measured after 90 days of treatment. The results showed that the 8R∶2B treatment was beneficial to the elongation of plant height，leaf growth and the increase of stem diameter of D.officinale test⁃tube seedlings.The number of leaves and qN were highest in blue light treatment with 7.846，0.861.The antioxidant index was the best in 5R∶5B treatment as a whole，and the dry weight，fresh weight，nutrient content，chlorophyll content and chlorophyll fluorescence of test⁃tube seedlings were also the best in the 5R∶5B treatment.there were significant correlations among growth index，antioxidant enzyme index，nutrient index，chlorophyll fluorescence indexes and chlorophyll content.The results of principal component analysis showed that the physiological growth and nutrient accumulation of D.officinale test⁃tube seedlings with 5R∶5B treatment were the best，and the comprehensive score was 3.12.In conclusion，when the D.officinale test⁃tube seedlings are in the LED light quality environment of 5R∶5B treatment，it is most conducive to their growth and development and nutrient accumulation.

Key words: Dendrobium officinale, LED, Light quality, Physiology biochemistry, Nutrient, Chlorophyll fluorescence

摘要： 为探究不同LED光质对铁皮石斛试管苗生长发育、光合作用及营养成分积累的影响，以铁皮石斛试管苗为材料，设置6种不同的光质处理［100%红光（R）、100%蓝光（B）、80%红光+20%蓝光（8R∶2B）、70%红光+30%蓝光（7R∶3B）、50%红光+50%蓝光（5R∶5B），以白色荧光灯为对照（CK）］，在处理90 d后测定生长指标（株高、叶片数、叶长、茎粗、鲜质量、干质量）、抗氧化指标［超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、抗坏血酸过氧化物酶（APX）活性、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性］、光合指标（叶绿素a含量、叶绿素b含量、叶绿素a+b含量和叶绿素a/b比值及类胡萝卜素含量）、叶绿素荧光指标（Fv/Fm、ΦPSⅡ、ETRⅡ、qN、qP、Fv/Fo）及营养成分（氨基酸、生物碱、多糖）含量。结果表明，8R∶2B处理有利于铁皮石斛试管苗株高伸长、叶片生长及茎粗增加。叶片数、qN均以B处理为最高，分别达7.846、0.861。抗氧化指标整体上以5R∶5B处理最优，试管苗干质量、鲜质量、营养物质和叶绿素含量及叶绿素荧光等指标亦整体上以5R∶5B处理最优。生长指标、抗氧化酶指标、营养指标、叶绿素荧光参数、叶绿素含量间存在显著相关关系。主成分分析结果表明，5R∶5B处理铁皮石斛试管苗生理生长及营养物质积累最优，综合得分达3.12。综上，铁皮石斛试管苗处于5R∶5B LED光质环境时，最利于其生长发育及营养成分积累。

关键词: 铁皮石斛, LED, 光质, 生理生化, 营养物质, 叶绿素荧光

CLC Number:

S567.239

LIN Qiongyu, ZHENG Lifang, MENG Xinya, WANG Jiangning, FU Qiunuan, SONG Xiqiang. Effects of LED Light Quality on Physiology，Biochemistry and Nutrient Content of Dendrobium officinale Test⁃tube Seedlings[J]. Journal of Henan Agricultural Sciences, 2025, 54(2): 66-76.

林琼瑜, 郑礼方, 孟新亚, 王江宁, 符秋暖, 宋希强. LED 光质对铁皮石斛试管苗生理生化及营养物质含量的影响[J]. 河南农业科学, 2025, 54(2): 66-76.

Figures/Tables 9

References

［1］SOLTANI S，AROUIEE H，SALEHI R，et al.Morphological，phytochemical，and photosynthetic performance of grafted tomato seedlings in response to different LED light qualities under protected cultivation［J］.Horticulturae，2023，9（4）：471.

［2］BOTTIGLIONE B，VILLANI A，MASTROPASQUA L，et al.Blue and red LED lights differently affect growth responses and biochemical parameters in lentil（Lens culinaris）［J］.Biology，2023，13（1）：12.

［3］LI Q Z，XU J X，YANG L Y，et al.LED light quality affect growth，alkaloids contents，and expressions of Amaryllidaceae alkaloids biosynthetic pathway genes in Lycoris longituba［J］.Journal of Plant Growth Regulation，2022，41（1）：257⁃270.

［4］LI X B，YANG K G，MA J，et al.Transcriptome analysis reveals different red and blue light duration promote

growth and main medicinal ingredients of Dendrobium nobile Lindl［J］.Industrial Crops and Products，2023，204：117347.

［5］LOBIUC A，VASILACHE V，PINTILIE O，et al.Blue and red LED illumination improves growth and bioactive compounds contents in acyanic and cyanic Ocimum basilicum L. microgreens［J］.Molecules，2017，22（12）：2111.

［6］RAHMAN M M，VASILIEV M，ALAMEH K. LED illumination spectrum manipulation for increasing the yield of sweet basil（Ocimum basilicum L.）［J］. Plants，2021，10（2）：344.

［7］HE J，QIN L，CHONG E L C，et al. Plant growth and photosynthetic characteristics of Mesembryanthemum crystallinum grown aeroponically under different blue⁃and red⁃LEDs［J］. Frontiers in Plant Science，2017，8：361.

［8］ZHANG T，SHI Y Y，PIAO F Z，et al.Effects of different LED sources on the growth and nitrogen metabolism of lettuce［J］. Plant Cell，Tissue and Organ Culture（PCTOC），2018，134（2）：231⁃240.

［9］李青竹，蔡友铭，杨贞，等.不同LED光质对石蒜幼苗生长、生理和生物碱积累的影响［J］.应用与环境生物学报，2019，25（6）：1414⁃1419.

LI Q Z，CAI Y M，YANG Z，et al. Effects of the quality of LED light on the growth，physiological characteristics，and the accumulation of alkaloids in Lycoris radiata［J］.Chinese Journal of Applied and Environmental Biology，2019，25（6）：1414⁃1419.

［10］GAO S，LIU X N，LIU Y，et al.Comparison of the effects of LED light quality combination on growth and nutrient accumulation in green onion（Allium fistulosum L.）［J］.Protoplasma，2021，258（4）：753⁃763.

［11］LIU N，JI F，XU L J，et al. Effects of LED light quality on the growth of pepper seedling in plant factory［J］.International Journal of Agricultural and Biological Engineering，2019，12（5）：44⁃50.

［12］XU L，ZENG X X，LIU Y N，et al. Effect of Dendrobium officinale polysaccharides on central nervous system

disease：Based on gut microbiota［J］.International Journal of Biological Macromolecules， 2023， 240：124440.

［13］周桂芬，庞敏霞，陈素红，等. 铁皮石斛茎、叶多糖含量及多糖部位柱前衍生化-高效液相色谱指纹图谱比较研究［J］. 中国中药杂志，2014，39（5）：795⁃802.

ZHOU G F，PANG M X，CHEN S H，et al. Comparison on polysaccharide content and PMP⁃HPLC fingerprints

of polysaccharide in stems and leaves of Dendrobium officinale［J］.Zhongguo Zhong Yao Za Zhi，2014，39 （5）：795⁃802.

［14］ JIAO C Y，WEI M K，FAN H H，et al.Transcriptomic analysis of genes related to alkaloid biosynthesis and

the regulation mechanism under precursor and methyl jasmonate treatment in Dendrobium officinale［J］.Frontiers in Plant Science，2022，13：941231.

［15］林丹霓，黄君婷，钟玉鸣，等. 兜唇石斛发酵多肽抗氧化活性与氨基酸结构的关系［J］.现代食品科技，2023，39（10）：139⁃150.

LIN D N，HUANG J T，ZHONG Y M，et al.Relationship between the oxidative activity and amino acid structure of fermented polypeptides from Dendrobium aphyllum［J］.Modern Food Science and Technology，2023，39（10）：139⁃150.

［16］FAN C Y， MANIVANNAN A， WEI H. Light quality⁃mediated influence of morphogenesis in micropropagated horticultural crops：A comprehensive overview［J］.BioMed Research International，2022，2022（1）：4615079.

［17］MORAŃSKA E，SIMLAT M，WARCHOŁ M，et al.Phenolic acids and Amaryllidaceae alkaloids profiles in Leucojum aestivum L.in vitro plants grown under different light conditions［J］. Molecules，2023，28（4）：1525.

［18］于鹏澎，宋金修，蔡玮，等. 夜间LED补光光照度和补光时间对番茄种苗质量的影响［J］. 江苏农业学报，2023，39（9）：1917⁃1926.

YU P P，SONG J X，CAI W，et al. Effects of LED supplementary light intensity and supplementary light time at night on seedling quality of tomato in the controlled environment ［J］. Jiangsu Journal of Agricultural Sciences，2023，39（9）：1917⁃1926.

［19］李合生. 植物生理生化实验原理和技术［M］. 北京：高等教育出版社，2000：36⁃43.

LI H S. Principle and technology of plant physiological and biochemical experiment［M］.Beijing：HigherEducation Press，2000：36⁃43.

［20］NAKANO Y，ASADA K. Hydrogen peroxide is scavenged by ascorbate⁃specific peroxidase in spinach chloroplasts［J］. Plant and Cell Physiology，1981，22 （5）：867⁃880.

［21］AEBI H. Catalase in vitro［J］. Methods in Enzymology，1984，5（1）：121⁃126.

［22］陈建勋，王晓峰. 植物生理学实验指导［M］. 2版. 广州：华南理工大学出版社，2006：56⁃78.

CHENG J X，WANG X F. Plant physiology experiment instruction［M］.2nd ed. Guangzhou：South China University of Technology Press，2006：56⁃78.

［23］王学奎. 植物生理生化实验原理和技术［M］.2版. 北京：高等教育出版社，2006：89⁃93.
WANG X K. Plant physiological and biochemical test principle and technology［M］.2nd ed. Beijing：Education Press，2006：89⁃93.

［24］LICHTENTHALER H K，BUSCHMANN C. Chlorophylls and carotenoids：Measurement and characterization by UV⁃VIS spectroscopy［J］. Current Protocols in Food Analytical Chemistry，2001，1（1）：1⁃8.

［25］KUREPIN L V，PHARIS R P. Light signaling and the phytohormonal regulation of shoot growth［J］.Plant Science，2014，229：280⁃289.

［26］HE R，GAO M F，SHI R，et al. The combination of selenium and LED light quality affects growth and nutritional properties of broccoli sprouts［J］. Molecules，2020，25（20）：4788.

［27］王丽伟，李岩，辛国凤，等. 不同比例红蓝光对番茄幼苗生长和光合作用的影响［J］.应用生态学报，2017，28（5）：1595⁃1602.
WANG L W，LI Y，XIN G F，et al .Effects of different proportions of red and blue light on the growth and photosynthesis of tomato seedlings［J］. Chinese Journal of Applied Ecology，2017，28（5）：1595⁃1602.

［28］ASLAMI Z，GHEHSAREH M G，MAHDAVI S M E，et al. Regeneration of African violet in response to light quality［J］. Horticulturae，2024，10（1）：78.

［29］WANG S L，LIU X T，LIU X N，et al. The red/blue light ratios from light⁃emitting diodes affect growth and flower quality of Hippeastrum hybridum‘Red Lion’［J］. Frontiers in Plant Science，2022，13：1048770.

［30］马文蝶，蒲珊，刘云洁，等. 光质和光强调控对芦笋幼苗形态建成和营养积累的影响［J］.浙江农业科学，2023，64（5）：1210⁃1214.
MA W D，PU S，LIU Y J，et al. Effects of light quality and light intensity regulation on morphogenesis and nutrient accumulation of Asparagus seedlings［J］.Journal of Zhejiang Agricultural Sciences，2023，64 （5）：1210⁃1214.

［31］HUNG C D，HONG C H，KIM S K，et al. LED light for in vitro and ex vitro efficient growth of economically important highbush blueberry（Vaccinium corymbosum L.）［J］. Acta Physiologiae Plantarum，2016，38（6）：152.

［32］GONG H，HU X R，ZHANG J，et al. Effect of red and blue light supplementation on the efficacy of Noccaea caerulescens in decontaminating metals and alleviating leaching risk［J］.Environmental Geochemistry and Health，2024，46（2）：48.

［33］AHMADI T，SHABANI L，SABZALIAN M R. LED light mediates phenolic accumulation and enhances antioxidant activity in Melissa officinalis L.under drought stress condition［J］.Protoplasma，2020，257 （4）：1231⁃1242.

［34］LIN K H，HUANG M Y，HUANG W D，et al.The effects of red，blue，and white light⁃emitting diodes on the growth， development， and edible quality of hydroponically grown lettuce（Lactuca sativa L.var.capitata）［J］.Scientia Horticulturae，2013，150：86⁃91.

［35］LI S C. Novel insight into functions of ascorbate peroxidase in higher plants：More than a simple antioxidant enzyme［J］. Redox Biology，2023，64：102789.

［36］胡举伟，郑磊，孟鑫，等. 不同红蓝光配比对樱桃幼苗形态发育、抗坏血酸和活性氧代谢的影响［J］.中国果树，2024（4）：55⁃60.
HU J W，ZHENG L，MENG X，et al. Effects of different proportions of red and blue light on morphological development and metabolism of ascorbic acid and reactive oxygen species in cherry seedlings［J］. China Fruits，2024（4）：55⁃60.

［37］CHEN H，LI Q P，ZENG Y L，et al. Effect of different shading materials on grain yield and quality of rice［J］.Scientific Reports，2019，9（1）：9992.

［38］王露璐，马飞，和苗苗.光氮互作对植物生长发育及氮素代谢影响的研究进展［J］.杭州师范大学学报（自然科学版），2024，23（1）：63⁃74.
WANG L L，MA F，HE M M. Review on effects of light and nitrogen interaction on plant growth and nitrogen metabolism［J］. Journal of Hangzhou Normal University（Natural Science Edition），2024，23（1）：63⁃74.

［39］杨鹏. 比较转录组分析揭示蓝光和红光对毛竹的调控作用［D］. 福州：福建农林大学，2022.
YANG P. Comparative transcriptome analysis reveals the regulatory effects of blue light and red light on Phyllostachys pubescens［D］.Fuzhou： Fujian Agriculture and Forestry University，2022.

［40］魏相飞. 石斛生长补光灯具设计：85632475. 6 ［P］. 2002⁃02⁃03.
WEI X F，Dendrobium growth fill light lamp design：85632475. 6［P］. 2020⁃02⁃03.

［41］闻永慧，孟英，李慧敏，等.LED不同光质对白及组培苗生长及可溶性糖含量的影响［J］.北方园艺，2014（15）：58⁃62.
WEN Y H，MENG Y，LI H M，et al. Effect of LED different light qualities on growth and the content of soluble sugar of bletiua Striata tissue culture seedlings［J］. Northern Horticulture，2014（15）：58⁃62.

［42］刘迪林，符恭震，王丰，等. 水稻快速育种技术的研究进展［J］. 广东农业科学，2023，50（12）：1⁃11.
LIU D L，FU G Z，WANG F，et al. Research progress of speed breeding technology in rice（Oryza sativa L.）［J］.Guangdong Agricultural Sciences，2023，50（12）：1⁃11.

［43］苗妍秀. 黄瓜幼苗对红蓝光质响应的生理机制［D］.北京：中国农业大学，2015.
MIAO Y X. Physiological mechanism of response of cucumber seedlings to red and blue light［D］. Beijing：China Agricultural University，2015.

［44］兰明忠. LED光质对竹柏幼苗生长和光合作用的影响［J］.亚热带农业研究，2019，15（3）：184⁃188.
LAN M Z.Effect of LED lights quality on the growth and photosynthesis of Nageia nagi seedlings［J］.Subtropical Agriculture Research，2019，15（3）：184⁃188.

［45］刘文科，杨其长，邱志平，等. LED光质对豌豆苗生长、光合色素和营养品质的影响［J］.中国农业气象，2012，33（4）：500⁃504.
LIU W K，YANG Q C，QIU Z P，et al. Effects of LED light quality on growth，photosynthetic pigments and nutritional quality of pea seedlings［J］. Chinese Journal of Agrometeorology，2012，33（4）：500⁃504.

［46］CHEN Q Q，ZHANG C Y，CHEN Y K，et al.Transcriptomic analysis for diurnal temperature differences reveals gene⁃regulation⁃network response to accumulation of bioactive ingredients of protocorm⁃like bodies in Dendrobium officinale［J］. Plants，2024，13 （6）：874.

［47］张松彦，迭鹏翔，宋梦婷，等. 烟草过表达刺槐RpACBP3对光合生理的影响［J］.园艺学报，2024，51 （9）：2155⁃2167.
ZHANG S Y，DIE P X，SONG M T，et al.Effect of overexpression of Robinia pseudoacacia RpACBP3 gene on photosynthetic physiological characteristics of Nicotiana tabacum［J］.Acta Horticulturae Sinica，2024，51（9）：2155⁃2167.

［48］MIAO Y X，WANG X Z，GAO L H，et al. Blue light is more essential than red light for maintaining the activities of photosystem Ⅱ and Ⅰ and photosynthetic electron transport capacity in cucumber leaves［J］.Journal of Integrative Agriculture，2016，15（1）：87⁃100.

［49］WANG Y，TONG Y F，CHU H L，et al. Effects of different light qualities on seedling growth and chlorophyll fluorescence parameters of Dendrobium officinale［J］. Biologia，2017，72（7）：735⁃744.

［50］张舵，刘有春，魏鑫，等. pH值对蓝莓生长及生理影响的机制解析［J］.江苏农业科学，2024，52（14）：1⁃9.
ZHANG D，LIU Y C，WEI X，et al.Mechanism analysis of the effect of pH value on the growth and physiology of blueberry［J］.Jiangsu Agricultural Sciences，2024，52（14）：1⁃9.

［51］魏帛轩，吴艾轩，徐瑞蔓，等. 不同光质补光处理对人参光合作用和产量的影响［J］.延边大学农学学报，2021，43（1）：7⁃17.
WEI B X，WU A X，XU R M，et al. Effects of different light quality supplemental light treatments on photosynthesis and yield of ginseng［J］.Agricultural Science Journal of Yanbian University，2021，43（1）：7⁃17.

［52］罗媛，叶林，郭文忠，等. 红蓝光补光对设施番茄生长发育及荧光特性的影响［J］.中国农业气象，2024，45（5）：506⁃516.
LUO Y，YE L，GUO W Z，et al. Influence by supplementary red and blue light on the growth and development of greenhouse tomatoes and fluorescence characteristics［J］.Chinese Journal of Agrometeorology，2024，45（5）：506⁃516.

［53］OUZOUNIS T，FRETTÉ X，OTTOSEN C O，et al.Spectral effects of LEDs on chlorophyll fluorescence and pigmentation in Phalaenopsis ‘Vivien’ and ‘Purple Star’［J］.Physiologia Plantarum，2015，154 （2）：314⁃327.

［54］GUO Y X，ZHONG Y F，MO L W，et al. Different combinations of red and blue LED light affect the growth，physiology metabolism and photosynthesis of in vitro⁃cultured Dendrobium nobile ‘Zixia’［J］.Horticulture，Environment，and Biotechnology，2023，64 （3）：393⁃407.

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