Screening，Identification，and in Vitro Antagonistic Mechanism Analysis of Strains against Phytophthora nicotianae

doi:10.15933/j.cnki.1004-3268.2026.05.009

Abstract

Abstract: To screen strains with antagonistic activity against Phytophthora nicotianae and to develop microbial resources for the biological control of tobacco black shank，antagonistic strains were isolated using dilution plating and dual‐culture assays. Dominant antagonistic strains were identified based on morphological characteristics，16S rRNA gene，and the sequences of 31 housekeeping genes.Their antimicrobial capacity was further evaluated via broad‐spectrum inhibition assays and determination of antibacterial activity in sterile fermentation filtrate.The control efficacy of sterile fermentation filtrates against P.nicotianae was assessed by measuring lesion length on detached leaves.Whole‐genome sequencing of the antagonistic strain was performed using next‐generation sequencing technology，and the genomic data were analyzed with KEGG database and antiSMASH software.Active antimicrobial compounds were identified by ultra‐performance liquid chromatography coupled with high‐resolution mass spectrometry（UPLC‐MS）. Results indicated that a strain of Bacillus cereus YC5 with antagonistic activity against P.nicotianae was successfully isolated and screened.Dual‐culture assays demonstrated that strain YC5 exhibited a 74.22% inhibition rate against P.nicotianae，along with antifungal activity against multiple plant pathogenic fungi including the causal agents of tobacco Fusarium wilt and wheat crown rot.In vitro leaf tests confirmed that the sterile fermentation filtrate of YC5 significantly suppressed lesion expansion，demonstrating remarkable antifungal efficacy.Genomic analysis revealed that YC5 possessed a genome size of 5 859 938 bp with 34.779% GC content.Among the 5 824 predicted genes，3 813 were annotated in the KEGG database.antiSMASH predicted 14 secondary metabolite biosynthetic gene clusters，and UPLC‐MS identified various upregulated antimicrobial compounds.These findings demonstrated that YC5 exerted its biocontrol activity primarily through the production of diverse antimicrobial substances，which effectively suppressed the growth of P.nicotianae and other phytopathogens，thereby providing protective effects for plants.In summary，strain YC5 has the ability to synthesize multiple antibacterial compounds，and has antagonistic effects on Phytophthora nicotianae and various plant pathogenic fungi.

Key words: Tobacco black shank, Phytophthora nicotianae, Antagonistic strain, Biological control, Bacillus cereus, Genome sequencing, Metabolnomics

摘要： 为筛选具有烟草疫霉拮抗活性的菌株，开发烟草黑胫病生防菌菌种资源，采用稀释涂布法、对峙培养法筛选拮抗菌株，结合形态学、16S rRNA基因和31个看家基因序列鉴定优势拮抗菌株，通过广谱抑菌鉴定、无菌发酵滤液抑菌活性测定探究拮抗菌株的抑菌能力，通过离体叶片病斑长度研究拮抗菌株无菌发酵滤液对烟草黑胫病的防治效果，借助二代测序技术对拮抗菌株进行基因组测序，结合KEGG、antiSMASH对拮抗菌株的基因组数据进行分析，利用超高效液相色谱-质谱联用技术（UPLC-MS）鉴定有效抗菌成分。结果表明，分离筛选出一株具有烟草疫霉拮抗活性的蜡样芽孢杆菌（Bacillus cereus）YC5。平板对峙发现，YC5对烟草疫霉的抑制率达到74.22%，且对烟草枯萎病病原菌、小麦茎基腐病病原菌等多种植物病原真菌具有抑菌能力。离体叶片防效结果表明，YC5无菌发酵滤液能显著抑制病斑长度，表现出优异的抗真菌活性。基因组数据分析结果表明，YC5基因组长度为5 859 938 bp，平均GC含量为34.779%，得到5 824个预测基因，其中有3 813个基因被注释到KEGG数据库，antiSMASH预测到14个次级代谢产物合成基因簇，在发酵液上调表达物中筛选鉴定出多种抗菌物质，表明YC5可通过分泌抗菌物质抑制烟草疫霉及多种病原菌生长，从而为植物提供保护。综上，菌株YC5具有合成多种抗菌化合物的能力，对烟草疫霉及多种植物病原真菌具有拮抗作用。

关键词: 烟草黑胫病, 烟草疫霉, 拮抗菌株, 生物防治, 蜡样芽胞杆菌, 基因组测序, 代谢组学

CLC Number:

S435.72
S476

ZHANG Zhigao, WANG Chuang, YIN Dong, HUANG Xumin, ZHANG Huifen, XU Xinghui, HE Wenqing, ZHANG Hong. Screening，Identification，and in Vitro Antagonistic Mechanism Analysis of Strains against Phytophthora nicotianae[J]. Journal of Henan Agricultural Sciences, 2026, 55(5): 83-95.

张志高, 王闯, 尹冬, 黄旭民, 章慧芬, 许兴辉, 何文卿, 张红. 烟草疫霉拮抗菌株的筛选、鉴定及体外拮抗机制分析[J]. 河南农业科学, 2026, 55(5): 83-95.

Figures/Tables 12

References

［1］陈希，胡丰晓. 两株抑制烟草疫霉的海洋源木霉的分离、鉴定及发酵条件优化［J］. 微生物学通报，2025，52（3）：1180‐1192.

CHEN X，HU F X. Isolation，identification，and fermentation condition optimization of two marine Trichoderma strains antagonizing Phytophthora parasitica［J］. Microbiology China，2025，52（3）：1180‐1192.

［2］付克剑，崔永和，黄智华，等. 抗烟草黑胫病菌生防细菌筛选、鉴定及体外拮抗机理初探［J］. 南方农业学报，2023，54（8）：2245‐2256.

FU K J，CUI Y H，HUANG Z H，et al. Screening，identification and antagonistic mechanism in vitro of biocontrol bacteria against tobacco black shank pathogen［J］. Journal of Southern Agriculture，2023，54（8）：2245‐2256.

［3］赵亚南，黄大野，杨丹，等. 烟草黑胫病研究进展［J］. 湖北农业科学，2022，61（S1）：25‐28.

ZHAO Y N，HUANG D Y，YANG D，et al. Research progress of tobacco black shank disease［J］. Hubei Agricultural Sciences，2022，61（S1）：25‐28.

［4］钏有聪，张立猛，焦永鸽，等. 大蒜与烤烟轮作对烟草黑胫病的防治效果及作用机理初探［J］. 中国烟草学报，2016，22（5）：55‐62.

CHUAN Y C，ZHANG L M，JIAO Y G，et al. Control effects of tobacco and garlic rotation on tobacco black shank and a preliminary study on the inhibition mechanism［J］. Acta Tabacaria Sinica，2016，22（5）：55‐62.

［5］孟祥佳，刘洋，黎妍妍，等. 烟草-荞麦轮作对烟草黑胫病防治及土壤微生态的调控作用［J］. 南方农业学报，2022，53（6）：1525‐1535.

MENG X J，LIU Y，LI Y Y，et al. Effects of tobacco‐buckwheat rotation on regulation of tobacco black shank prevention and control and soil microenvironment［J］. Journal of Southern Agriculture，2022，53（6）：1525‐1535.

［6］曾维爱，周志成，谭琳，等. 湖南烟草病虫害绿色防控技术研究现状与应用对策［J］. 中国烟草学报，2019，25（2）：69‐73.

ZENG W A，ZHOU Z C，TAN L，et al. Research status and application of green prevention and control technology against tobacco diseases and pests in Hunan［J］. Acta Tabacaria Sinica，2019，25（2）：69‐73.

［7］王亚月，贾方方，常栋，等. 烟草黑胫病拮抗菌的筛选鉴定及抑菌活性分析［J］. 烟草科技，2025，58（1）：52‐60.

WANG Y Y，JIA F F，CHANG D，et al. Screening，identification and bacteriostatic activity of a bacterial strain against tobacco black shank［J］. Tobacco Science & Technology，2025，58（1）：52‐60.

［8］殷全玉，匡志豪，王景，等. 黑胫病不同抗性烤烟品种对哈茨木霉的生理响应［J］. 河南农业科学，2022，51（9）：88‐98.

YIN Q Y，KUANG Z H，WANG J，et al. Physiological responses of different black shank‐resistance flue‐cured tobacco varieties to Trichoderma harzianum［J］. Journal of Henan Agricultural Sciences，2022，51（9）：88‐98.

［9］王亚月，贾方方，李俊营，等. 烟草黑胫病拮抗菌的筛选鉴定与防病促生作用研究［J］. 中国烟草科学，2022，43（6）：60‐67.

WANG Y Y，JIA F F，LI J Y，et al. Screening，identification of a bacterial strain against tobacco black shank and its growth‐promoting effects［J］. Chinese Tobacco Science，2022，43（6）：60‐67.

［10］HAN T，YOU C，ZHANG L，et al. Biocontrol potential of antagonist Bacillus subtilis Tpb55 against tobacco black shank［J］. BioControl，2016，61（2）：195‐205.

［11］董国菊，马冠华，肖崇刚. 荧光假单胞菌拮抗菌株对烟草疫霉的抑菌机制及控病效果［J］. 植物保护学报，2012，39（2）：115‐120.

DONG G J，MA G H，XIAO C G. Antagonistic mechanism of Pseudomonas fluorescens strains against Phytophthora nicotianae and biocontrol effect on tobacco black shank［J］. Journal of Plant Protection，2012，39（2）：115‐120.

［12］梅斯钇，滕凯，肖志鹏，等. 烟草疫霉拮抗细菌M10-4 的筛选及其生防和促生效果［J］. 江苏农业科学，2024，52（24）：133‐140.

MEI S Y，TENG K，XIAO Z P，et al. Screening of antagonistic bacteria M10‐4 against Phytophthora nicotianae and its biocontrol and growth‐promoting effects［J］. Jiangsu Agricultural Sciences，2024，52 （24）：133‐140.

［13］刘天波，赵誉强，滕凯，等. 烟草疫霉拮抗细菌B44R-1 的筛选及其生防效果［J］. 中国生物防治学报，2022，38（6）：1558‐1565.

LIU T B，ZHAO Y Q，TENG K，et al. Screening and biocontrol effect of antagonistic bacterium B44R‐1 against Phytophthora parasitica［J］. Chinese Journal of Biological Control，2022，38（6）：1558‐1565.

［14］东秀珠，蔡妙英. 常见细菌系统鉴定手册［M］. 北京：科学出版社，2001.

DONG X Z，CAI M Y. Manual for identification of common bacterial systems［M］. Beijing：Science Press，2001.

［15］夏北成，ZHOU J Z，TIEDJE J M. 分子生物学方法在微生物生态学中的应用［J］. 中山大学学报（自然科学版），1998（2）：98‐102.

XIA B C，ZHOU J Z，TIEDJE J M. Application of molecular methods in microbial ecology［J］. Acta Scientiarum Naturalium Universitatis Sunyatseni，1998（2）：98‐102.

［16］李慧，郑肖兰，侯会霞，等. 铜绿假单胞菌PaHNHK01 的鉴定及其对剑麻烟草疫霉的生防效果研究［J］. 热带作物学报，2024，45（10）：2171‐2182.

LI H，ZHENG X L，HOU H X，et al. Identification and biocontrol of Pseudomonas aeruginosa PaHNHK01 against sisal zebra disease［J］. Chinese Journal of Tropical Crops，2024，45（10）：2171‐2182.

［17］XU R，SONG S Q，WU G C，et al. Biocontrol ability of Arcopilus aureus YZXR against Fusarium fujikuroi causing leaf spot on Polygonatum odoratum［J］.Phytopathology Research，2024，6（1）：68.

［18］朱畇昊，彭淑萍，赵乐，等. 地黄促生内生尖孢镰刀菌GG22基因组结构分析［J］. 江苏农业科学，2021，49（19）：72‐77.

ZHU Y H，PENG S P，ZHAO（L /Y），et al. Genomic structure analysis of plant growth‐promoting endophytic Fusarium oxysporum GG22 in Rehmannia glutinosa［J］. Jiangsu Agricultural Sciences，2021，49 （19）：72‐77.

［19］BLIN K，SHAW S，KLOOSTERMAN A M，et al.antiSMASH 6. 0：Improving cluster detection and comparison capabilities［J］. Nucleic Acids Research，2021，49（W1）：W29‐W35.

［20］MOON K，HWANG S，LEE H J，et al. Identification of the antibacterial action mechanism of diterpenoids through transcriptome profiling ［J］. Frontiers in Microbiology，2022，13：945023.

［21］BAUER R，DICKS L M T. Mode of action of lipid Ⅱ‐targeting lantibiotics［J］. International Journal of Food Microbiology，2005，101（2）：201‐216.

［22］MECCATTI V M，SANTOS L F，DE CARVALHO L S，et al. Antifungal action of herbal plants’ glycolic extracts against Candida species［J］. Molecules，2023，28（6）：2857.

［23］ZHANG C S，FENG C，ZHENG Y F，et al. Root exudates metabolic profiling suggests distinct defense mechanisms between resistant and susceptible tobacco cultivars against black shank disease［J］. Frontiers in Plant Science，2020，11：559775.

［24］LIU S C，LI Z Y，ZHANG L Y，et al. Antibacterial and quorum‐sensing inhibitory activities of Alpinia galanga（L.）Willd essential oil against Aeromonas dhakensis and Shewanella seohaensis［J］. South African Journal of Botany，2025，182：112‐119.

［25］朱玉涛，薛冰怡，王豫蓝，等. 生防菌防治棉花黄萎病的研究进展［J］. 山西农业科学，2026，54（1）：29‐38.

ZHU Y T，XUE B Y，WANG Y L，et al. Research progress on biocontrol bacteria for cotton Verticillium wilt［J］. Journal of Shanxi Agricultural Sciences，2026，54（1）：29‐38.

［26］杨康，张可，吴阔，等. 3种植物精油主要成分及其对烟草疫霉的抑菌活性［J］. 南方农业学报，2025，56（10）：3208‐3219.

YANG K，ZHANG K，WU K，et al. Main components of three plant essential oils and their antimicrobial activity on Phytophthora nicotianae［J］.Journal of Southern Agriculture，2025，56（10）：3208‐3219.

［27］高强，王丽丽，张渐隆，等. 高地芽孢杆菌CY1的分离鉴定及其对烟草黑胫病的防治作用［J］. 浙江农业学报，2025，37（2）：405‐416.

GAO Q，WANG L L，ZHANG J L，et al. Screening and identification of Bacillus altitudinis strain CY1 and its control effects against tobacco black shank［J］. Acta Agriculturae Zhejiangensis，2025，37（2）：405‐416.

［28］李海利，徐引弟，王治方，等. 胶冻样芽孢杆菌对革兰氏阴性菌的抑菌活性及其抗菌次级代谢产物分析［J］. 河南农业科学，2024，53（10）：159‐169.

LI H L，XU Y D，WANG Z F，et al. Analysis of antibacterial activity and secondary metabolites of Paenibacillus kribbensis against gram‐negative bacteria ［J］. Journal of Henan Agricultural Sciences，2024，53 （10）：159‐169.

［29］邓聪，马璐，汪青松，等. 芽孢杆菌对盐碱胁迫下玉米种子萌发及生理生化特性的影响［J］. 河南农业科学，2025，54（3）：20‐30.
DENG C，MA L，WANG Q S，et al. Effect of Bacillus on seed germination and physiological and biochemical
characteristics of maize under salt‐alkali stress［J］.Journal of Henan Agricultural Sciences，2025，54（3）：20‐30.

［30］邢孔政，施春兰，杨德伟，等. 1株贝莱斯芽孢杆菌的生物学特性测定及发酵条件优化［J］. 南方农业学报，2025，56（11）：3419‐3431.
XING K Z，SHI C L，YANG D W，et al.Biological characteristics determination and fermentation condition optimization of a strain of Bacillus velezensis［J］. Journal of Southern Agriculture，2025，56（11）：3419‐3431.

［31］张林林，谢太震，马肖静，等. 生物炭与多黏类芽孢杆菌配施对辣椒根际土壤微生态的影响［J］. 河南农业大学学报，2026，60（1）：96‐109.
ZHANG L L，XIE T Z，MA X J，et al.The effect of combined application of biochar and Paenibacillus polymyxa on the microecology of pepper rhizosphere soil［J］. Journal of Henan Agricultural University，2026，60（1）：96‐109.

［32］梁振普，贾俊卿，王秋云，等.柽柳根际喜盐芽孢杆菌Bachu 85的分离及耐盐促生功能鉴定［J］. 河南农业大学学报，2025，59（3）：444‐454.
LIANG Z P，JIA J Q，WANG Q Y，et al. Isolation and identification of salt‐tolerant growth promoting ability of Halobacillus Bachu 85 from rhizosphere of Tamarix chinensis［J］. Journal of Henan Agricultural University，2025，59（3）：444‐454.

［33］SILO‐SUH L A，LETHBRIDGE B J，RAFFEL S J，et al. Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85［J］. Applied and Environmental Microbiology，1994，60（6）：2023‐2030.

［34］LIU Z Y，ZHAO Y T，HUANG C Q，et al. Recent advances in silent gene cluster activation in Streptomyces［J］.Frontiers in Bioengineering and Biotechnology，2021，9：632230.

［35］白天天，万颖，张延龙，等. 屎肠球菌F11. 1G比较基因组、代谢组学分析及代谢产物挖掘［J］. 微生物学通报，2025，52（6）：2769‐2788.
BAI T T，WAN Y，ZHANG Y L，et al. Comparative genomics analysis， metabolomics analysis，and metabolite mining of Enterococcus faecium F11.1G［J］.Microbiology China，2025，52（6）：2769‐2788.

［36］WANG J，ZHANG L，TENG K L，et al. Cerecidins，novel lantibiotics from Bacillus cereus with potent antimicrobial activity［J］. Applied and Environmental Microbiology，2014，80（8）：2633‐2643.

［37］FARZAND A，MOOSA A，ZUBAIR M，et al. Suppression of Sclerotinia sclerotiorum by the induction of systemic resistance and regulation of antioxidant pathways in tomato using fengycin produced by Bacillus amyloliquefaciens FZB42［J］. Biomolecules，2019，9（10）：613.

［38］PHELAN R W，BARRET M，COTTER P D，et al.Subtilomycin：A new lantibiotic from Bacillus subtilis strain MMA7 isolated from the marine sponge Haliclona simulans［J］. Marine Drugs，2013，11（6）：1878‐1898.

［39］DENG Y，CHEN H Q，LI C Z，et al. Endophyte Bacillus subtilis evade plant defense by producing lantibiotic subtilomycin to mask self‐produced flagellin［J］. Communications Biology，2019，2：368.

［40］MANCK L E，PARK J，TULLY B J，et al. Petrobactin，a siderophore produced by Alteromonas，mediates community iron acquisition in the global ocean［J］.The ISME Journal，2022，16（2）：358‐369.

［41］DIMOPOULOU A，THEOLOGIDIS I，BENAKI D，et al.Direct antibiotic activity of bacillibactin broadens the biocontrol range of Bacillus amyloliquefaciens MBI600［J］. mSphere，2021，6（4）：e00376‐e00321.

［42］李海利，张婉琪，徐引弟，等. 莫海威芽孢杆菌对革兰氏阳性菌的抑制活性及其抗菌次级代谢产物分析［J］. 河南农业科学，2024，53（12）：149‐158.
LI H L，ZHANG W Q，XU Y D，et al. Analysis of inhibitory activity of Bacillus mojavensis against Grampositive bacteria and its antibacterial secondary metabolites ［J］. Journal of Henan Agricultural Sciences，2024，53（12）：149‐158.

［43］KULKOVA I，DOBRZYŃSKI J，KOWALCZYK P，et al.Plant growth promotion using Bacillus cereus［J］.International Journal of Molecular Sciences，2023，24 （11）：9759.

［44］SCHLÜNZEN F，PYETAN E，FUCINI P，et al. Inhibition of peptide bond formation by pleuromutilins：The structure of the 50S ribosomal subunit from Deinococcus radiodurans in complex with tiamulin［J］. Molecular Microbiology，2004，54（5）：1287‐1294.

［45］SHIGETOMI K，SHOJI K，MITSUHASHI S，et al. The antibacterial properties of 6‐tuliposide B.Synthesis of 6‐tuliposide B analogues and structure‐activity relationship［J］. Phytochemistry，2010，71（2/3）：312‐324.

［46］CHEN Y H，LU M H，GUO D S，et al. Antifungal effect of magnolol and honokiol from Magnolia officinalis on Alternaria alternata causing tobacco brown spot［J］.Molecules，2019，24（11）：2140.

［47］BLAŽENOVIĆ I，KIND T，SA M R，et al. Structure annotation of all mass spectra in untargeted metabolomics［J］. Analytical Chemistry，2019，91（3）：2155‐2162.

［48］MACHADO H，TUTTLE R N，JENSEN P R.Omics‐based natural product discovery and the lexicon of genome mining［J］. Current Opinion in Microbiology，2017，39：136‐142.

［49］VINAIXA M，SCHYMANSKI E L，NEUMANN S，et al.Mass spectral databases for LC/MS‐ and GC/MS‐based metabolomics：State of the field and future prospects［J］. TrAC Trends in Analytical Chemistry，2016，78：23‐35.

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