Metabolite Differences and Anthocyanin Quantitative Analysis of Black Wolfberry from Different Regions

doi:10.15933/j.cnki.1004-3268.2026.01.007

Abstract

Abstract: In order to clarify the differences in metabolite composition and anthocyanin content among black wolfberries（Lycium ruthenicum）from different origins，so as to provide a basis for quality evaluation and origin identification of black wolfberries，based on ultra‐high‐performance liquid chromatography‐tandem mass spectrometry（UHPLC‐MS/MS） technology combined with multivariate statistical analysis，the metabolic differences of black wolfberries from three origins，namely，Qaidam Basin in Qinghai Province（QHL），Linzhi City in Tibet Autonomous Region（XZL），and Shenmu City in Shaanxi Province（SXL），were systematically compared，and a quantitative analysis method of anthocyanin was established for determination and comparison. The results showed that a total of 21 categories and 629 metabolites were identified in the fruits of black wolfberries from the three origins，with flavonoids，amino acids and their derivatives，and organic acids and their derivatives accounting for a relatively higher proportion.Principal component analysis（PCA）and orthogonal partial least squares discriminant analysis（OPLS‐DA）results indicated significant differences in the metabolism of black wolfberries from the three origins.The screening results of differential metabolites showed that there were 494 differential metabolites between SXL and QHL black wolfberries，111 between XZL and QHL black wolfberries，and 478 between XZL and SXL.The quantitative analysis results of anthocyanins showed that，calculated as delphinidin‐3‐O‐glucoside（DPG）equivalents，the total anthocyanin content of QHL black wolfberries was the highest，at 49.71 mg/g；XZL was second，at 37.58 mg/g；and SXL was the lowest，at 32.23 mg/g.The KEGG enrichment analysis results indicated that the differential metabolites were mainly enriched in pathways related to tryptophan metabolism，amino acid biosynthesis，and flavonoid biosynthesis.In summary，there were significant differences in the metabolites of black wolfberry fruits from Qinghai，Tibet，and Shaanxi，with the highest anthocyanin content in Qinghai origin，and these differences were mainly enriched in pathways related to amino acid and flavonoid biosynthesis.

Key words: Black wolfberry, Metabolite, Multivariate statistical analysis, Anthocyanin, Quantitative analysis

摘要： 为明确不同产地黑枸杞在代谢物组成及花青素含量上的差异，以便为黑枸杞品质评价和产地鉴别提供依据，基于超高效液相色谱-串联质谱（UHPLC-MS/MS）技术，结合多元统计分析，系统比较青海省柴达木盆地（QHL）、西藏自治区林芝市（XZL）和陕西省神木市（SXL）3个产地黑枸杞的代谢物差异，并建立花青素定量分析方法进行测定比较。结果表明，3个产地黑枸杞果实中共鉴定出21类629种代谢产物，其中黄酮类、氨基酸及其衍生物和有机酸及其衍生物占比较高。主成分分析（PCA）和正交偏最小二乘法-判别分析（OPLS-DA）结果显示，3个产地黑枸杞代谢谱存在明显差异。差异代谢物筛选结果表明，SXL与QHL黑枸杞之间存在494种差异代谢物，XZL与QHL黑枸杞之间存在111种差异代谢物，XZL与SXL之间存在478种差异代谢物。花青素定量分析结果表明，以飞燕草素-3-O-葡萄糖苷（DPG）当量计，QHL黑枸杞总花青素含量最高，为49.71 mg/g；XZL次之，为37.58 mg/g；SXL最低，为32.23 mg/g。KEGG富集分析结果表明，差异代谢物主要富集于色氨酸代谢、氨基酸生物合成和黄酮类生物合成通路。综上，青海、西藏、陕西三地黑枸杞果实代谢物存在明显差异，其中青海产地的花青素含量最高，这些差异主要富集于氨基酸和黄酮类生物合成通路。

关键词: 黑枸杞, 代谢物质, 多元统计分析, 花青素, 定量分析

CLC Number:

S567
Q946.92

WU Jingjie, LIU Hongxia. Metabolite Differences and Anthocyanin Quantitative Analysis of Black Wolfberry from Different Regions[J]. Journal of Henan Agricultural Sciences, 2026, 55(1): 76-87.

武婧洁, 刘红霞. 不同产地黑枸杞代谢物差异及花青素定量分析[J]. 河南农业科学, 2026, 55(1): 76-87.

Figures/Tables 6

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